myeloproliferative leukemia virus oncogene (MPL) - coding DNA reference sequence

(used for mutation description)

(last modified May 1, 2014)


This file was created to facilitate the description of sequence variants in the MPL gene based on a coding DNA reference sequence following the HGVS recommendations.

The sequence was taken from NC_000001.10, covering MPL transcript NM_005373.2.


Please note that introns are available by clicking on the exon numbers above the sequence.
 (upstream sequence)
                     .         .         .         .                g.1045
                cctgaagggaggatgggctaaggcaggcacacagtggcggagaag       c.-1

          .         .         .         .         .         .       g.1105
 ATGCCCTCCTGGGCCCTCTTCATGGTCACCTCCTGCCTCCTCCTGGCCCCTCAAAACCTG       c.60
 M  P  S  W  A  L  F  M  V  T  S  C  L  L  L  A  P  Q  N  L         p.20

          .          | 02        .         .         .         .    g.1336
 GCCCAAGTCAGCAGCCAAG | ATGTCTCCTTGCTGGCATCAGACTCAGAGCCCCTGAAGTGT    c.120
 A  Q  V  S  S  Q  D |   V  S  L  L  A  S  D  S  E  P  L  K  C      p.40

          .         .         .         .         .         .       g.1396
 TTCTCCCGAACATTTGAGGACCTCACTTGCTTCTGGGATGAGGAAGAGGCAGCGCCCAGT       c.180
 F  S  R  T  F  E  D  L  T  C  F  W  D  E  E  E  A  A  P  S         p.60

          .         .         .   | 03     .         .         .    g.1766
 GGGACATACCAGCTGCTGTATGCCTACCCGCG | GGAGAAGCCCCGTGCTTGCCCCCTGAGT    c.240
 G  T  Y  Q  L  L  Y  A  Y  P  R  |  E  K  P  R  A  C  P  L  S      p.80

          .         .         .         .         .         .       g.1826
 TCCCAGAGCATGCCCCACTTTGGAACCCGATACGTGTGCCAGTTTCCAGACCAGGAGGAA       c.300
 S  Q  S  M  P  H  F  G  T  R  Y  V  C  Q  F  P  D  Q  E  E         p.100

          .         .         .         .         .         .       g.1886
 GTGCGTCTCTTCTTTCCGCTGCACCTCTGGGTGAAGAATGTGTTCCTAAACCAGACTCGG       c.360
 V  R  L  F  F  P  L  H  L  W  V  K  N  V  F  L  N  Q  T  R         p.120

          .         .         .  | 04      .         .         .    g.2496
 ACTCAGCGAGTCCTCTTTGTGGACAGTGTAG | GCCTGCCGGCTCCCCCCAGTATCATCAAG    c.420
 T  Q  R  V  L  F  V  D  S  V  G |   L  P  A  P  P  S  I  I  K      p.140

          .         .         .         .         .         .       g.2556
 GCCATGGGTGGGAGCCAGCCAGGGGAACTTCAGATCAGCTGGGAGGAGCCAGCTCCAGAA       c.480
 A  M  G  G  S  Q  P  G  E  L  Q  I  S  W  E  E  P  A  P  E         p.160

          .         .         .         .         .         .       g.2616
 ATCAGTGATTTCCTGAGGTACGAACTCCGCTATGGCCCCAGAGATCCCAAGAACTCCACT       c.540
 I  S  D  F  L  R  Y  E  L  R  Y  G  P  R  D  P  K  N  S  T         p.180

          .         .         .         .         .         .       g.2676
 GGTCCCACGGTCATACAGCTGATTGCCACAGAAACCTGCTGCCCTGCTCTGCAGAGGCCT       c.600
 G  P  T  V  I  Q  L  I  A  T  E  T  C  C  P  A  L  Q  R  P         p.200

          .         .         .         .         .         .       g.2736
 CACTCAGCCTCTGCTCTGGACCAGTCTCCATGTGCTCAGCCCACAATGCCCTGGCAAGAT       c.660
 H  S  A  S  A  L  D  Q  S  P  C  A  Q  P  T  M  P  W  Q  D         p.220

          .         .         . | 05       .         .         .    g.3190
 GGACCAAAGCAGACCTCCCCAAGTAGAGAA | GCTTCAGCTCTGACAGCAGAGGGTGGAAGC    c.720
 G  P  K  Q  T  S  P  S  R  E   | A  S  A  L  T  A  E  G  G  S      p.240

          .         .         .         .         .         .       g.3250
 TGCCTCATCTCAGGACTCCAGCCTGGCAACTCCTACTGGCTGCAGCTGCGCAGCGAACCT       c.780
 C  L  I  S  G  L  Q  P  G  N  S  Y  W  L  Q  L  R  S  E  P         p.260

          .         .         .         .         .         .       g.3310
 GATGGGATCTCCCTCGGTGGCTCCTGGGGATCCTGGTCCCTCCCTGTGACTGTGGACCTG       c.840
 D  G  I  S  L  G  G  S  W  G  S  W  S  L  P  V  T  V  D  L         p.280

          .    | 06    .         .         .         .         .    g.3630
 CCTGGAGATGCAG | TGGCACTTGGACTGCAATGCTTTACCTTGGACCTGAAGAATGTTACC    c.900
 P  G  D  A  V |   A  L  G  L  Q  C  F  T  L  D  L  K  N  V  T      p.300

          .         .         .         .         .         .       g.3690
 TGTCAATGGCAGCAACAGGACCATGCTAGCTCCCAAGGCTTCTTCTACCACAGCAGGGCA       c.960
 C  Q  W  Q  Q  Q  D  H  A  S  S  Q  G  F  F  Y  H  S  R  A         p.320

          .         . | 07       .         .         .         .    g.9681
 CGGTGCTGCCCCAGAGACAG | GTACCCCATCTGGGAGAACTGCGAAGAGGAAGAGAAAACA    c.1020
 R  C  C  P  R  D  R  |  Y  P  I  W  E  N  C  E  E  E  E  K  T      p.340

          .         .         .         .         .         .       g.9741
 AATCCAGGACTACAGACCCCACAGTTCTCTCGCTGCCACTTCAAGTCACGAAATGACAGC       c.1080
 N  P  G  L  Q  T  P  Q  F  S  R  C  H  F  K  S  R  N  D  S         p.360

          .         .         .         .         .         .       g.9801
 ATTATTCACATCCTTGTGGAGGTGACCACAGCCCCGGGTACTGTTCACAGCTACCTGGGC       c.1140
 I  I  H  I  L  V  E  V  T  T  A  P  G  T  V  H  S  Y  L  G         p.380

          .         .      | 08  .         .         .         .    g.10023
 TCCCCTTTCTGGATCCACCAGGCTG | TGCGCCTCCCCACCCCAAACTTGCACTGGAGGGAG    c.1200
 S  P  F  W  I  H  Q  A  V |   R  L  P  T  P  N  L  H  W  R  E      p.400

          .         .         .         .         .         .       g.10083
 ATCTCCAGTGGGCATCTGGAATTGGAGTGGCAGCACCCATCGTCCTGGGCAGCCCAAGAG       c.1260
 I  S  S  G  H  L  E  L  E  W  Q  H  P  S  S  W  A  A  Q  E         p.420

          .         .         .         .         | 09         .    g.12051
 ACCTGTTATCAACTCCGATACACAGGAGAAGGCCATCAGGACTGGAAG | GTGCTGGAGCCG    c.1320
 T  C  Y  Q  L  R  Y  T  G  E  G  H  Q  D  W  K   | V  L  E  P      p.440

          .         .         .         .         .         .       g.12111
 CCTCTCGGGGCCCGAGGAGGGACCCTGGAGCTGCGCCCGCGATCTCGCTACCGTTTACAG       c.1380
 P  L  G  A  R  G  G  T  L  E  L  R  P  R  S  R  Y  R  L  Q         p.460

          .         .         .         .         .         .       g.12171
 CTGCGCGCCAGGCTCAACGGCCCCACCTACCAAGGTCCCTGGAGCTCGTGGTCGGACCCA       c.1440
 L  R  A  R  L  N  G  P  T  Y  Q  G  P  W  S  S  W  S  D  P         p.480

          .         .         | 10         .         .         .    g.12491
 ACTAGGGTGGAGACCGCCACCGAGACCG | CCTGGATCTCCTTGGTGACCGCTCTGCATCTA    c.1500
 T  R  V  E  T  A  T  E  T  A |   W  I  S  L  V  T  A  L  H  L      p.500

          .         .         .         .         .         .       g.12551
 GTGCTGGGCCTCAGCGCCGTCCTGGGCCTGCTGCTGCTGAGGTGGCAGTTTCCTGCACAC       c.1560
 V  L  G  L  S  A  V  L  G  L  L  L  L  R  W  Q  F  P  A  H         p.520

       | 11  .         .         .         .         .         .    g.15467
 TACAG | GAGACTGAGGCATGCCCTGTGGCCCTCACTTCCAGACCTGCACCGGGTCCTAGGC    c.1620
 Y  R  |  R  L  R  H  A  L  W  P  S  L  P  D  L  H  R  V  L  G      p.540

          .         .         .    | 12    .         .         .    g.15741
 CAGTACCTTAGGGACACTGCAGCCCTGAGCCCG | CCCAAGGCCACAGTCTCAGATACCTGT    c.1680
 Q  Y  L  R  D  T  A  A  L  S  P   | P  K  A  T  V  S  D  T  C      p.560

          .         .         .         .         .         .       g.15801
 GAAGAAGTGGAACCCAGCCTCCTTGAAATCCTCCCCAAGTCCTCAGAGAGGACTCCTTTG       c.1740
 E  E  V  E  P  S  L  L  E  I  L  P  K  S  S  E  R  T  P  L         p.580

          .         .         .         .         .         .       g.15861
 CCCCTGTGTTCCTCCCAGGCCCAGATGGACTACCGAAGATTGCAGCCTTCTTGCCTGGGG       c.1800
 P  L  C  S  S  Q  A  Q  M  D  Y  R  R  L  Q  P  S  C  L  G         p.600

          .         .         .         .         .         .       g.15921
 ACCATGCCCCTGTCTGTGTGCCCACCCATGGCTGAGTCAGGGTCCTGCTGTACCACCCAC       c.1860
 T  M  P  L  S  V  C  P  P  M  A  E  S  G  S  C  C  T  T  H         p.620

          .         .         .         .                           g.15969
 ATTGCCAACCATTCCTACCTACCACTAAGCTATTGGCAGCAGCCTTGA                   c.1908
 I  A  N  H  S  Y  L  P  L  S  Y  W  Q  Q  P  X                     p.635

          .         .         .         .         .         .       g.16029
 ggacaggctcctcactcccagttccctggacagagctaaactctcgagacttctctgtga       c.*60

          .         .         .         .         .         .       g.16089
 acttccctaccctacccccacaacacaagcaccccagacctcacctccatccccctctgt       c.*120

          .         .         .         .         .         .       g.16149
 ctgccctcacaattaggcttcattgcactgatcttactctactgctgctgacataaaacc       c.*180

          .         .         .         .         .         .       g.16209
 aggaccctttctccacaggcaggctcatttcactaagctcctcctttactttctctctcc       c.*240

          .         .         .         .         .         .       g.16269
 tctttgatgtcaaacgccttgaaaacaagcctccacttccccacacttcccatttactct       c.*300

          .         .         .         .         .         .       g.16329
 tgagactacttcaattagttcccctactacactttgctagtgaaactgcccaggcaaagt       c.*360

          .         .         .         .         .         .       g.16389
 gcacctcaaatcttctaattccaagatccaataggatctcgttaatcatcagttcctttg       c.*420

          .         .         .         .         .         .       g.16449
 atctcgctgtaagatttgtcaaggctgactactcacttctcctttaaattctttcctacc       c.*480

          .         .         .         .         .         .       g.16509
 ttggtcctgcctctttgagtatattagtaggttttttttatttgtttgagacagggtctc       c.*540

          .         .         .         .         .         .       g.16569
 actctgtcacccaggctgcagtgcaatggcgcgatctcagctcactgcaacctccacctc       c.*600

          .         .         .         .         .         .       g.16629
 cgggttcaagcgattcttgtgcctcggcctccctagtagctgggattacaggcgcacacc       c.*660

          .         .         .         .         .         .       g.16689
 accacacacagctaattttttttttttttttttttttttttttttagacggagccttgct       c.*720

          .         .         .         .         .         .       g.16749
 ctgttgccagactggagtgcagtggcacgatctcggctcactgcaacctctgcctcccgg       c.*780

          .         .         .         .         .         .       g.16809
 gttcaagccattctgcctcagcctcccaagtagctgggagtacaggcgtctgccaccatg       c.*840

          .         .         .         .         .         .       g.16869
 cctaatttttttctatttttaggagagaccggttttcaccacgttggccaggatggtctc       c.*900

          .         .         .         .         .         .       g.16929
 gatatcctgatctcgtgatccgcctgcctctgcctcccaaagtgctgggattacaggtgt       c.*960

          .         .         .         .         .         .       g.16989
 gacccactgcgcacagccccagctaattttcatatttttagtagagacagggttttgcca       c.*1020

          .         .         .         .         .         .       g.17049
 tgttgcccaggctggtcttgaactcctaacctcgggtgatccacccaccttggcctccca       c.*1080

          .         .         .         .         .         .       g.17109
 aagtgttaggattacaggcatgagccactgcgcccggctgagtgtactagtagttaagag       c.*1140

          .         .         .         .         .         .       g.17169
 aataaactagatctagaatcagagctggattcaattcctgtccttcacatttactagctg       c.*1200

          .         .         .         .         .         .       g.17229
 tgcaaccttgggcacataacttaatgtctttgagccttagttttttcatctgtaaaacag       c.*1260

          .         .         .         .         .         .       g.17289
 ggataataacagcaccccatagagttgtgacgaggattgagataatctaagtaaagcaca       c.*1320

          .         .         .         .         .         .       g.17349
 gtccctaggacatagtaaatgattcatatatccgaactactgttataattattccttctt       c.*1380

          .         .         .         .         .         .       g.17409
 actctcctcttctagcatttcttccaattattacagtccttcaagattccatttcttaac       c.*1440

          .         .         .         .         .         .       g.17469
 agtctccaatcccatctattctctgcctttactatatgttgaccattccaaagttcttat       c.*1500

          .         .         .         .         .         .       g.17529
 ctctagctcagacatctactacagcactgtgatgctttatgcaactaactgtttacatat       c.*1560

          .         .         .         .         .         .       g.17589
 ctgtcccctgctactagattgtgagctccttgagggaaaggaacatgatttatttgtcct       c.*1620

          .         .         .         .         .         .       g.17649
 tttcccccagcacctagagtagtgcttggtgcatgatagtaggccttcaataaatttttt       c.*1680

          .                                                         g.17661
 ctaaatgaatga                                                       c.*1692

 (downstream sequence)
Legend:
Nucleotide numbering (following the rules of the HGVS for a 'Coding DNA Reference Sequence') is indicated at the right of the sequence, counting the A of the ATG translation initiating Methionine as 1. Every 10th nucleotide is indicated by a "." above the sequence. The Myeloproliferative leukemia virus oncogene protein sequence is shown below the coding DNA sequence, with numbering indicated at the right starting with 1 for the translation initiating Methionine. Every 10th amino acid is shown in bold. The position of introns is indicated by a vertical line, splitting the two exons. The start of the first exon (transcription initiation site) is indicated by a '\', the end of the last exon (poly-A addition site) by a '/'. The exon number is indicated above the first nucleotide(s) of the exon. To aid the description of frame shift mutations, all stop codons in the +1 frame are shown in bold while all stop codons in the +2 frame are underlined.

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