Confocal Imaging of Donor 16171.B06 Containing an ABCA3 Homozygous Missense Mutation for SFTPC, KI67, and ACTA2

Confocal Imaging of Donor 16171.B06 Containing an ABCA3 Homozygous Missense Mutation for SFTPC, KI67, and ACTA2

Immunofluorescence- SFTPC, Ki-67and ACTA2

 

Purpose:  Immunofluorescence on slides of 5µm paraffin sections of human lung containing ABCA3 homozygous missense mutations  SFTPC, Ki-67and ACTA2 with antigen retrieval.

Day 1

  1. Paraffin sections are placed at 60oC for 2 hours to overnight to melt paraffin.
  2. Para