{"id":17488,"date":"2017-08-14T17:01:54","date_gmt":"2017-08-14T17:01:54","guid":{"rendered":"https:\/\/research.cchmc.org\/lungimage\/?p=17488"},"modified":"2018-10-18T12:07:19","modified_gmt":"2018-10-18T12:07:19","slug":"3-year-human-lung-ager-pecam-1and-nkx2-1-confocal-imaging-2","status":"publish","type":"post","link":"https:\/\/research.cchmc.org\/lungimage\/?p=17488","title":{"rendered":"3 Year Human Lung AGER, PECAM-1,and NKX2.1 Confocal Imaging"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"17488\" class=\"elementor elementor-17488 elementor-bc-flex-widget\" data-elementor-post-type=\"post\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-489d668b elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"489d668b\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-38f81475\" data-id=\"38f81475\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-633f998c elementor-widget elementor-widget-theme-post-title elementor-page-title elementor-widget-heading\" data-id=\"633f998c\" data-element_type=\"widget\" data-widget_type=\"theme-post-title.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h1 class=\"elementor-heading-title elementor-size-large\">3 Year Human Lung AGER, PECAM-1,and NKX2.1 Confocal Imaging<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-51691b5f elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"51691b5f\" data-element_type=\"section\">\n\t\t\t\t\t\t<div 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class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2017\/08\/2017-06-05_LMH-17-D039-LLL-6A3.16_AGER_PECAM1_NKX2.1-01.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2017\/08\/2017-06-05_LMH-17-D039-LLL-6A3.16_AGER_PECAM1_NKX2.1-01.jpg\" alt=\"Confocal Imaging Showing Protein Expression of AGER, PECAM1, and NKX2.1 Genes\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">Confocal Imaging Showing Protein Expression of AGER, PECAM1, and NKX2.1 Genes<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2017\/08\/2017-06-05_LMH-17-D039-LLL-6A3.16_AGER_PECAM1_NKX2.1-02.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2017\/08\/2017-06-05_LMH-17-D039-LLL-6A3.16_AGER_PECAM1_NKX2.1-02.jpg\" alt=\"Confocal Imaging Showing Protein Expression of AGER, PECAM1, and NKX2.1 Genes\" data-description=\"60X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">Confocal Imaging Showing Protein Expression of AGER, PECAM1, and NKX2.1 Genes<\/div><div class=\"html5gallery-description\">60X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2017\/08\/2017-06-05_LMH-17-D039-LLL-6A3.16_AGER_PECAM1_NKX2.1-03.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2017\/08\/2017-06-05_LMH-17-D039-LLL-6A3.16_AGER_PECAM1_NKX2.1-03.jpg\" alt=\"Confocal Imaging Showing Protein Expression of AGER, PECAM1, and NKX2.1 Genes\" data-description=\"60X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">Confocal Imaging Showing Protein Expression of AGER, PECAM1, and NKX2.1 Genes<\/div><div class=\"html5gallery-description\">60X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2017\/08\/2017-06-05_LMH-17-D039-LLL-6A3.16_AGER_PECAM1_NKX2.1-07.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2017\/08\/2017-06-05_LMH-17-D039-LLL-6A3.16_AGER_PECAM1_NKX2.1-07.jpg\" alt=\"Confocal Imaging Showing Protein Expression of AGER, PECAM1, and NKX2.1 Genes\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">Confocal Imaging Showing Protein Expression of AGER, PECAM1, and NKX2.1 Genes<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2017\/08\/2017-06-05_LMH-17-D039-LLL-6A3.16_AGER_PECAM1_NKX2.1-06.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2017\/08\/2017-06-05_LMH-17-D039-LLL-6A3.16_AGER_PECAM1_NKX2.1-06.jpg\" alt=\"Confocal Imaging Showing Protein Expression of AGER, PECAM1, and NKX2.1 Genes\" data-description=\"60X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">Confocal Imaging Showing Protein Expression of AGER, PECAM1, and NKX2.1 Genes<\/div><div class=\"html5gallery-description\">60X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2017\/08\/2017-06-01_LMH-17-D032-LLL-8A2.11_AGER_PECAM1_NKX2.1-06.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2017\/08\/2017-06-01_LMH-17-D032-LLL-8A2.11_AGER_PECAM1_NKX2.1-06.jpg\" alt=\"Confocal Imaging Showing Protein Expression of AGER, PECAM1, and NKX2.1 Genes\" data-description=\"60X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">Confocal Imaging Showing Protein Expression of AGER, PECAM1, and NKX2.1 Genes<\/div><div class=\"html5gallery-description\">60X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2017\/08\/2017-06-05_LMH-17-D039-LLL-6A3.16_AGER_PECAM1_NKX2.1-07.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2017\/08\/2017-06-05_LMH-17-D039-LLL-6A3.16_AGER_PECAM1_NKX2.1-07.jpg\" alt=\"Confocal Imaging Showing Protein Expression of AGER, PECAM1, and NKX2.1 Genes\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">Confocal Imaging Showing Protein Expression of AGER, PECAM1, and NKX2.1 Genes<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2017\/08\/2017-06-05_LMH-17-D039-LLL-6A3.16_AGER_PECAM1_NKX2.1-08.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2017\/08\/2017-06-05_LMH-17-D039-LLL-6A3.16_AGER_PECAM1_NKX2.1-08.jpg\" alt=\"Confocal Imaging Showing Protein Expression of AGER, PECAM1, and NKX2.1 Genes\" data-description=\"60X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">Confocal Imaging Showing Protein Expression of AGER, PECAM1, and NKX2.1 Genes<\/div><div class=\"html5gallery-description\">60X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2017\/08\/2017-06-05_LMH-17-D039-LLL-6A3.16_AGER_PECAM1_NKX2.1-09.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2017\/08\/2017-06-05_LMH-17-D039-LLL-6A3.16_AGER_PECAM1_NKX2.1-09.jpg\" alt=\"Confocal Imaging Showing Protein Expression of AGER, PECAM1, and NKX2.1 Genes\" data-description=\"60X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">Confocal Imaging Showing Protein Expression of AGER, PECAM1, and NKX2.1 Genes<\/div><div class=\"html5gallery-description\">60X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2017\/08\/2017-06-05_LMH-17-D039-LLL-6A3.16_AGER_PECAM1_NKX2.1-10.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2017\/08\/2017-06-05_LMH-17-D039-LLL-6A3.16_AGER_PECAM1_NKX2.1-10.jpg\" alt=\"Confocal Imaging Showing Protein Expression of AGER, PECAM1, and NKX2.1 Genes\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">Confocal Imaging Showing Protein Expression of AGER, PECAM1, and NKX2.1 Genes<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2017\/08\/2017-06-05_LMH-17-D039-LLL-6A3.16_AGER_PECAM1_NKX2.1-11.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2017\/08\/2017-06-05_LMH-17-D039-LLL-6A3.16_AGER_PECAM1_NKX2.1-11.jpg\" alt=\"Confocal Imaging Showing Protein Expression of AGER, PECAM1, and NKX2.1 Genes\" data-description=\"60X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">Confocal Imaging Showing Protein Expression of AGER, PECAM1, and NKX2.1 Genes<\/div><div class=\"html5gallery-description\">60X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2017\/08\/2017-06-05_LMH-17-D039-LLL-6A3.16_AGER_PECAM1_NKX2.1-12.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2017\/08\/2017-06-05_LMH-17-D039-LLL-6A3.16_AGER_PECAM1_NKX2.1-12.jpg\" alt=\"Confocal Imaging Showing Protein Expression of AGER, PECAM1, and NKX2.1 Genes\" data-description=\"60X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">Confocal Imaging Showing Protein Expression of AGER, PECAM1, and NKX2.1 Genes<\/div><div class=\"html5gallery-description\">60X<\/div><\/div><\/div><\/div><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-2469c7b5 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"2469c7b5\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-70e1fc7d\" data-id=\"70e1fc7d\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-53d42fe2 elementor-widget elementor-widget-template\" data-id=\"53d42fe2\" data-element_type=\"widget\" data-widget_type=\"template.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-template\">\n\t\t\t\t\t<div data-elementor-type=\"page\" data-elementor-id=\"27211\" class=\"elementor elementor-27211 elementor-bc-flex-widget\" data-elementor-post-type=\"elementor_library\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-4650a05 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"4650a05\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-951322a\" data-id=\"951322a\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-4f951ba\" data-id=\"4f951ba\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-0022067\" data-id=\"0022067\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-7e910b4 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"7e910b4\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-690e5fff\" data-id=\"690e5fff\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-49350831 elementor-widget elementor-widget-text-editor\" data-id=\"49350831\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>\u00a0\u00a0Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from\u00a0the University of Rochester Medical Center<\/p><p><a href=\"https:\/\/www.urmc.rochester.edu\/labs\/Pryhuber-Lab\/\" target=\"_blank\" rel=\"noopener noreferrer\"><img class=\"lazyload\" decoding=\"async\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27200%27%20height%3D%2748%27%20viewBox%3D%270%200%20200%2048%27%3E%3Crect%20width%3D%27200%27%20height%3D%2748%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2015\/07\/Pryhuber_Lab.jpg\" alt=\"Pryhuber_Lab\" width=\"200\" height=\"48\" \/><\/a><\/p><p style=\"text-align: center;\"><span style=\"font-size: 14pt;\"><strong>Immunofluorescence-\u00a0<\/strong><a href=\"https:\/\/www.rndsystems.com\/products\/human-rage-antibody_af1145\"><strong>AGER<\/strong><\/a><strong>,\u00a0<\/strong><a href=\"https:\/\/www.rndsystems.com\/products\/human-cd31-pecam-1-antibody-9g11_bba7\"><strong>PECAM1<\/strong><\/a><strong>, NKX2.1<\/strong><\/span><\/p><p><strong>\u00a0<\/strong><\/p><p><strong>Purpose:\u00a0\u00a0<\/strong>Stain slides of 7\u00b5m frozen sections of postnatal human lung from HTC for\u00a0<a href=\"https:\/\/www.rndsystems.com\/products\/human-rage-antibody_af1145\"><strong>AGER<\/strong><\/a><strong>,\u00a0<\/strong><a href=\"https:\/\/www.rndsystems.com\/products\/human-cd31-pecam-1-antibody-9g11_bba7\"><strong>PECAM1<\/strong><\/a><strong>, NKX2.1\u00a0<\/strong>with antigen retrieval.<\/p><p><strong>Day 1<\/strong><\/p><ol><li>For Frozen tissue, rinse 2X in PBS, then 5 min in 4% PFA\/PBS, then rinse 1X in PBS.<\/li><li>Briefly equilibrate slides in Antigen Retrieval Buffer.<\/li><li>Antigen retrieval, pH 6.0 (times will vary according to microwave).<\/li><li>10 mM sodium citrate, pH 6.0, and heat in a microwave at 96<sup>o<\/sup>C.\u00a0*We usually do a series of three runs (6-7 minutes each run) to equal the time\/temp because of evaporation (refill coplin jars with dH<sub>2<\/sub>O).<\/li><li>Microwave according to instructions on microwave.<\/li><li>Cool on countertop, 15 min.<\/li><li>Rinse with dH<sub>2<\/sub>O<\/li><li>1X PBS, 5 min.<\/li><li>Block in 4% Donkey serum\/PBS-T, 2 hours at RT.<\/li><li>For goat anti-<a href=\"https:\/\/www.rndsystems.com\/products\/human-rage-antibody_af1145\"><strong>AGER<\/strong><\/a>\u00a0(<a href=\"https:\/\/www.rndsystems.com\/products\/human-rage-antibody_af1145\"><strong>AF1145<\/strong><\/a>, R&amp;D Systems) dilute 1:100, For mouse anti-<a href=\"https:\/\/www.rndsystems.com\/products\/human-cd31-pecam-1-antibody-9g11_bba7\"><strong>PECAM1<\/strong><\/a>\u00a0(<a href=\"https:\/\/www.rndsystems.com\/products\/human-cd31-pecam-1-antibody-9g11_bba7\"><strong>CD31<\/strong><\/a>\u00a0,\u00a0<a href=\"https:\/\/www.rndsystems.com\/products\/human-cd31-pecam-1-antibody-9g11_bba7\"><strong>BBA7<\/strong><\/a>, R&amp;D Systems) dilute 1:100, For Rabbit anti-<strong>NKX2.1<\/strong>\u00a0(RB TTF-1 1231, Lot# 0912A) dilute 1:500 in blocking buffer. Spin down in \u00b5fuge for 10 min, apply to tissue and incubated O\/N @ 4<sup>o<\/sup>C.<\/li><\/ol><p><strong>Day 2<\/strong><\/p><ol><li>Rinse slides in PBS-T 3X, 5 min.<\/li><\/ol><ol start=\"2\"><li>Apply secondary antibody , Donkey Alexa Fluor 488 anti-goat IgG (<a href=\"http:\/\/www.lifetechnologies.com\/order\/catalog\/product\/A11055?ICID=search-a11057\"><strong>A11055<\/strong><\/a>, Lot# 1687906, anti-<a href=\"https:\/\/www.rndsystems.com\/products\/human-rage-antibody_af1145\"><strong>AGER<\/strong><\/a>\u00a0) at 1:200, Donkey Alexa Fluor 568 anti-mouse IgG (<a href=\"https:\/\/www.thermofisher.com\/antibody\/product\/Donkey-anti-Mouse-IgG-H-L-Secondary-Antibody-Polyclonal\/A-10037\"><strong>A10037<\/strong><\/a>, lot# 1752099, anti-<a href=\"https:\/\/www.rndsystems.com\/products\/human-cd31-pecam-1-antibody-9g11_bba7\"><strong>PECAM1<\/strong><\/a>) at 1:200, Donkey Alexa Fluor 647 anti-rabbit IgG (<a href=\"https:\/\/www.thermofisher.com\/antibody\/product\/Donkey-anti-Rabbit-IgG-H-L-Secondary-Antibody-Polyclonal\/A-31573\"><strong>A31573<\/strong><\/a>, anti-<strong>1<\/strong>) at 1:200 in blocking buffer. Spin down in \u00b5fuge for 10 min, apply to tissue and incubated at room temperature for 1 hour.<\/li><\/ol><ol><li>Rinse in PBS-T 3X, 5 min.<\/li><li>Dilute DAPI 1:2000 and apply to slides for 10 min.<\/li><li>Wash in PBS-T 3X, 5 min.<\/li><li>Rinse slides in 0.1M PB or TB, 3X, 5 min.<\/li><li>Add 1 drop of Prolong Gold anti-fade mounting medium (P36930).<\/li><li>Coverslip with Gold Seal Coverslip (Cat# 3422 Electron Microscopy Sciences, 22 X 22 mm).<\/li><li>Allow Prolong Gold to cure overnight at room temp in light sealed box.<\/li><li>Store slides in a light-sealed box at 4<sup>o<\/sup>C.<\/li><\/ol><p><strong>\u00a0<\/strong><\/p><p><strong style=\"letter-spacing: 0px;\">Tissue Used:\u00a0<br \/><\/strong><strong style=\"letter-spacing: 0px;\">LMH-D039-LLL-6A3.16<br \/><\/strong><strong style=\"letter-spacing: 0px;\">Gender: Female<br \/><\/strong><strong style=\"letter-spacing: 0px;\">Age: 39 Months<br \/><\/strong><strong style=\"letter-spacing: 0px;\">Gestational Age at Birth:\u00a0 23 weeks<\/strong><\/p><p><strong>\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/strong><\/p><p><strong style=\"letter-spacing: 0px;\">Appendix<br \/><\/strong><strong style=\"letter-spacing: 0px;\">Antigen Retrieval Solution<\/strong><\/p><p>18ml Solution A- 0.1M Citric Acid (Sigma, C1909) pH 2.5<\/p><p>82ml Reagent B- 0.1M Sodium Citrate (Sigma, S4641 ) pH 8.2<\/p><p>1L dH<sub>2<\/sub>O<\/p><p>pH 6.0<\/p><p>\u00a0<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-75cf4071 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"75cf4071\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-13910ce8\" data-id=\"13910ce8\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-44c77a55 elementor-widget elementor-widget-facebook-comments\" data-id=\"44c77a55\" data-element_type=\"widget\" data-widget_type=\"facebook-comments.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-facebook-widget fb-comments\" data-href=\"https:\/\/research.cchmc.org\/lungimage?p=17488\" data-width=\"100%\" data-numposts=\"10\" data-order-by=\"social\" style=\"min-height: 1px\"><\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-51167775\" data-id=\"51167775\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-60a1c8a5 elementor-widget elementor-widget-sidebar\" data-id=\"60a1c8a5\" data-element_type=\"widget\" data-widget_type=\"sidebar.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div id=\"search-64\" class=\"widget widget_search\">\t\t<form role=\"search\" class=\"searchform fusion-search-form  fusion-search-form-classic\" method=\"get\" action=\"https:\/\/research.cchmc.org\/lungimage\/\">\n\t\t\t<div class=\"fusion-search-form-content\">\n\n\t\t\t\t\n\t\t\t\t<div class=\"fusion-search-field search-field\">\n\t\t\t\t\t<label><span class=\"screen-reader-text\">Search for:<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<input type=\"search\" value=\"\" name=\"s\" class=\"s\" placeholder=\"Search...\" required aria-required=\"true\" aria-label=\"Search...\"\/>\n\t\t\t\t\t\t\t\t\t\t\t<\/label>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"fusion-search-button search-button\">\n\t\t\t\t\t<input type=\"submit\" class=\"fusion-search-submit searchsubmit\" aria-label=\"Search\" value=\"&#xf002;\" \/>\n\t\t\t\t\t\t\t\t\t<\/div>\n\n\t\t\t\t\n\t\t\t<\/div>\n\n\n\t\t\t\n\t\t<\/form>\n\t\t<\/div><div id=\"text-52\" class=\"widget widget_text\"><div class=\"heading\"><h4 class=\"widget-title\">Related Experiments<\/h4><\/div>\t\t\t<div class=\"textwidget\"><div class=\"wondercarousellightbox_options\" data-skinsfoldername=\"\"  data-jsfolder=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/plugins\/wonderplugin-carousel\/engine\/\" style=\"display:none;\"><\/div>\r\n<div class=\"wonderplugincarousel-container\" id=\"wonderplugincarousel-container-216\"><div class=\"wonderplugincarousel\" id=\"wonderplugincarousel-216\" data-carouselid=\"216\" data-width=\"350\" data-height=\"350\" data-skin=\"vertical\" data-showimgtitle=\"false\" data-sameheight=\"false\" data-sameheightresponsive=\"false\" data-fullwidth=\"false\" data-centerimage=\"false\" 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data-imgtitle=\"title\" data-circularlimit=\"3\" data-lightboxsocialposition=\"position:absolute;top:100%;right:0;\" data-lightboxsocialpositionsmallscreen=\"position:absolute;top:100%;right:0;left:0;\" data-lightboxsocialdirection=\"horizontal\" data-lightboxsocialbuttonsize=\"32\" data-lightboxsocialbuttonfontsize=\"18\" data-sameheightmediumscreen=\"769\" data-sameheightmediumheight=\"200\" data-sameheightsmallscreen=\"415\" data-sameheightsmallheight=\"150\" data-triggerresizedelay=\"100\" data-lightboxslideinterval=\"5000\" data-lightboxtimerposition=\"bottom\" data-lightboxtimercolor=\"#dc572e\" data-lightboxtimeropacity=\"1\" data-lightboxbordersize=\"8\" data-lightboxborderradius=\"0\" data-screenquery='{ \"mobile\": { \"screenwidth\": 600, \"visibleitems\": 1 } }' data-jsfolder=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/plugins\/wonderplugin-carousel\/engine\/\" style=\"display:none;position:relative;margin:0 auto;width:100%;max-width:350px;\" ><div class=\"amazingcarousel-list-container\" style=\"overflow:hidden;\"><ul class=\"amazingcarousel-list\"><li class=\"amazingcarousel-item\"><div class=\"amazingcarousel-item-container\"><div class=\"amazingcarousel-image\">\n\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=13103\" target=\"_blank\"><img decoding=\"async\" class=\"lazyload amazingcarousel-image-img\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27300%27%20height%3D%27300%27%20viewBox%3D%270%200%20300%20300%27%3E%3Crect%20width%3D%27300%27%20height%3D%27300%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2017\/03\/2016-10-05_LMH-15-D024-LLL-9B2.13_PECAM1_AGER_NKX2.1-04-1.jpg\" alt=\"4 Month Human Lung PECAM-1, AGER, and NKX2.1 Confocal Imaging\" data-description=\"\" \/><\/a>\n\t<div class=\"amazingcarousel-text\">\n\t\t<div class=\"amazingcarousel-text-bg\"><\/div>\n\t\t<div class=\"amazingcarousel-title\">4 Month Human Lung PECAM-1, AGER, and NKX2.1 Confocal Imaging<\/div>\n\t<\/div>\n<\/div><\/div><\/li><li class=\"amazingcarousel-item\"><div class=\"amazingcarousel-item-container\"><div class=\"amazingcarousel-image\">\n\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=13202\" target=\"_blank\"><img decoding=\"async\" class=\"lazyload amazingcarousel-image-img\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27300%27%20height%3D%27300%27%20viewBox%3D%270%200%20300%20300%27%3E%3Crect%20width%3D%27300%27%20height%3D%27300%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2017\/03\/2016-10-10_LMH-15-D003-LLL-4B3.21_PECAM1_AGER_NKX2.1-04-1.jpg\" alt=\"9 Month Human Lung PECAM-1, AGER, and NXK2.1 Confocal Imaging\" data-description=\"\" \/><\/a>\n\t<div class=\"amazingcarousel-text\">\n\t\t<div class=\"amazingcarousel-text-bg\"><\/div>\n\t\t<div class=\"amazingcarousel-title\">9 Month Human Lung PECAM-1, AGER, and NXK2.1 Confocal Imaging<\/div>\n\t<\/div>\n<\/div><\/div><\/li><li class=\"amazingcarousel-item\"><div class=\"amazingcarousel-item-container\"><div class=\"amazingcarousel-image\">\n\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=13164\" target=\"_blank\"><img decoding=\"async\" class=\"lazyload amazingcarousel-image-img\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27300%27%20height%3D%27300%27%20viewBox%3D%270%200%20300%20300%27%3E%3Crect%20width%3D%27300%27%20height%3D%27300%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2017\/03\/2016-10-06_LMH-15-D001-LLL-6B1.22_PECAM1_AGER_NKX2.1-01-1.jpg\" alt=\"20 Month Human Lung PECAM-1, AGER, and NKX2.1 Confocal Imaging\" data-description=\"\" \/><\/a>\n\t<div class=\"amazingcarousel-text\">\n\t\t<div class=\"amazingcarousel-text-bg\"><\/div>\n\t\t<div class=\"amazingcarousel-title\">20 Month Human Lung PECAM-1, AGER, and NKX2.1 Confocal Imaging<\/div>\n\t<\/div>\n<\/div><\/div><\/li><li class=\"amazingcarousel-item\"><div class=\"amazingcarousel-item-container\"><div class=\"amazingcarousel-image\">\n\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=13142\" target=\"_blank\"><img decoding=\"async\" class=\"lazyload amazingcarousel-image-img\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27300%27%20height%3D%27300%27%20viewBox%3D%270%200%20300%20300%27%3E%3Crect%20width%3D%27300%27%20height%3D%27300%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2017\/03\/2016-10-05_LMH-15-D032-LLL-8B2.15_PECAM1_AGER_NKX2.1-07-1.jpg\" alt=\"3 Year Human Lung LMH, PECAM1, AGER, NKX2.1 Confocal Imaging\" data-description=\"\" \/><\/a>\n\t<div class=\"amazingcarousel-text\">\n\t\t<div class=\"amazingcarousel-text-bg\"><\/div>\n\t\t<div class=\"amazingcarousel-title\">3 Year Human Lung LMH, PECAM1, AGER, NKX2.1 Confocal Imaging<\/div>\n\t<\/div>\n<\/div><\/div><\/li><li class=\"amazingcarousel-item\"><div class=\"amazingcarousel-item-container\"><div class=\"amazingcarousel-image\">\n\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=13182\" target=\"_blank\"><img decoding=\"async\" class=\"lazyload amazingcarousel-image-img\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27300%27%20height%3D%27300%27%20viewBox%3D%270%200%20300%20300%27%3E%3Crect%20width%3D%27300%27%20height%3D%27300%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2017\/03\/2016-10-06_LMH-15-D007-LLL-5D4.9_PECAM1_AGER_NKX2.1-07-1.jpg\" alt=\"4 Year Human Lung PECAM-1, AGER, and NKX2.1 Confocal Imaging\" data-description=\"\" \/><\/a>\n\t<div class=\"amazingcarousel-text\">\n\t\t<div class=\"amazingcarousel-text-bg\"><\/div>\n\t\t<div class=\"amazingcarousel-title\">4 Year Human Lung PECAM-1, AGER, and NKX2.1 Confocal Imaging<\/div>\n\t<\/div>\n<\/div><\/div><\/li><li class=\"amazingcarousel-item\"><div class=\"amazingcarousel-item-container\"><div class=\"amazingcarousel-image\">\n\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=21464\" target=\"_blank\"><img decoding=\"async\" class=\"lazyload amazingcarousel-image-img\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27300%27%20height%3D%27300%27%20viewBox%3D%270%200%20300%20300%27%3E%3Crect%20width%3D%27300%27%20height%3D%27300%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/04\/2017-09-28_LMH-17-D086-LLL-8A3.9_AGER_PECAM1_NKX2.1-09-1.jpg\" alt=\"8 Month-Old Human Lung Immunofluorescence and Confocal Imaging BPD Donor D086 Showing Expressions of AGER, PECAM1, and NKX2.1\" data-description=\"\" \/><\/a>\n\t<div class=\"amazingcarousel-text\">\n\t\t<div class=\"amazingcarousel-text-bg\"><\/div>\n\t\t<div class=\"amazingcarousel-title\">8 Month-Old Human Lung Immunofluorescence and Confocal Imaging BPD Donor D086 Showing Expressions of AGER, PECAM1, and NKX2.1<\/div>\n\t<\/div>\n<\/div><\/div><\/li><\/ul><div class=\"amazingcarousel-prev\"><\/div><div class=\"amazingcarousel-next\"><\/div><\/div><div class=\"amazingcarousel-nav\"><\/div><\/div><\/div><script>function wondercarousel_216_appendcss(csscode) {var head=document.head || document.getElementsByTagName(\"head\")[0];var style=document.createElement(\"style\");head.appendChild(style);style.type=\"text\/css\";if (style.styleSheet){style.styleSheet.cssText=csscode;} else {style.appendChild(document.createTextNode(csscode));}};wondercarousel_216_appendcss(\"@import url(https:\/\/fonts.googleapis.com\/css?family=Open+Sans); 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.amazingcarousel-bullet-wrapper { \tmargin: 16px; } .amazingcarousel-socialmedia-button { display: inline-block; margin: 4px; }.amazingcarousel-socialmedia-button a { box-shadow: none; }.amazingcarousel-socialmedia-icon { display:table-cell; width:32px; height:32px; font-size:18px; border-radius:50%; color:#fff; vertical-align:middle; text-align:center; cursor:pointer; padding:0;}.amazingcarousel-socialmedia-rotate { transition: transform .4s ease-in; } .amazingcarousel-socialmedia-rotate:hover { transform: rotate(360deg); }\");<\/script><\/div>\n\t\t<\/div>\n\t\t<div id=\"recent-posts-63\" class=\"widget widget_recent_entries\">\n\t\t<div class=\"heading\"><h4 class=\"widget-title\">Recent Experiments<\/h4><\/div>\n\t\t<ul>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=32066\">4 Year-Old Human Lung KRT14, MUC5B, and TUBA4A Confocal Imaging<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31223\">Alcian Blue Staining from PBR 3653 Patient with Cystic Fibrosis<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31202\">H&#038;E Staining from PBR 3383 Patient with Cystic Fibrosis<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31199\">H&#038;E Staining from PBR 3653 Patient with Cystic Fibrosis<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31196\">H&#038;E Staining of 31 Year-Old Human Donor 10<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31193\">H&#038;E Staining of 27 Year-Old Human Donor 3<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31190\">Alcian Blue Staining of 27 Year-Old Human Donor 3<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31186\">Alcian Blue Staining of 31 Year-Old Human Donor 10<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31182\">Alcian Blue Staining from PBR 3383 Patient with Cystic Fibrosis<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31024\">Alcian Blue Staining of 29 Year-Old Human Donor D175-10C1.21<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31019\">Alcian Blue Staining of 29 Year-Old Human Donor D175-11C4.14<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31016\">Alcian Blue Staining of 29 Year-Old Human Donor D175-11C2.11<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31013\">Alcian Blue Staining of 24 Year-Old Human Donor D071-23C2.19<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31010\">Alcian Blue Staining of 24 Year-Old Human Donor D071-17C2.20<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31007\">Alcian Blue Staining of 24 Year-Old Human Donor D071-16C3.27<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t<\/ul>\n\n\t\t<\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>\u00a0\u00a0Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from\u00a0the University of Rochester Medical Center Immunofluorescence-\u00a0AGER,\u00a0PECAM1, NKX2.1 \u00a0 Purpose:\u00a0\u00a0Stain slides of 7\u00b5m frozen sections of postnatal human lung from HTC for\u00a0AGER,\u00a0PECAM1, NKX2.1\u00a0with antigen retrieval. Day 1 For Frozen tissue, rinse 2X in PBS, then 5 min in 4% PFA\/PBS, then rinse 1X in PBS. Briefly equilibrate [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":17489,"comment_status":"closed","ping_status":"open","sticky":false,"template":"elementor_header_footer","format":"standard","meta":{"footnotes":""},"categories":[290,286,79],"tags":[108,84,22,85,129,36],"class_list":["post-17488","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-bpd-confocal-imaging","category-human-lung-disease-confocal-imaging","category-perinatalhuman","tag-ager","tag-cd31","tag-nkx2-1","tag-pecam-1","tag-rage","tag-ttf-1"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v25.6 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>3 Year Human Lung AGER, PECAM-1,and NKX2.1 Confocal Imaging -<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/research.cchmc.org\/lungimage\/?p=17488\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"3 Year Human Lung AGER, PECAM-1,and NKX2.1 Confocal Imaging -\" \/>\n<meta property=\"og:description\" content=\"\u00a0\u00a0Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from\u00a0the University of Rochester Medical Center Immunofluorescence-\u00a0AGER,\u00a0PECAM1, NKX2.1 \u00a0 Purpose:\u00a0\u00a0Stain slides of 7\u00b5m frozen sections of postnatal human lung from HTC for\u00a0AGER,\u00a0PECAM1, NKX2.1\u00a0with antigen retrieval. Day 1 For Frozen tissue, rinse 2X in PBS, then 5 min in 4% PFA\/PBS, then rinse 1X in PBS. Briefly equilibrate [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/research.cchmc.org\/lungimage\/?p=17488\" \/>\n<meta property=\"article:published_time\" content=\"2017-08-14T17:01:54+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2018-10-18T12:07:19+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2017\/08\/2017-06-05_LMH-17-D039-LLL-6A3.16_AGER_PECAM1_NKX2.1-03-1.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"300\" \/>\n\t<meta property=\"og:image:height\" content=\"300\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"kitc5i\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"kitc5i\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"2 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