{"id":20063,"date":"2018-03-16T17:27:11","date_gmt":"2018-03-16T17:27:11","guid":{"rendered":"https:\/\/research.cchmc.org\/lungimage\/?p=20063"},"modified":"2018-11-12T18:27:33","modified_gmt":"2018-11-12T18:27:33","slug":"2-year-old-human-lung-confocal-imaging-donor-d022-syp-sox2-and-tubb3","status":"publish","type":"post","link":"https:\/\/research.cchmc.org\/lungimage\/?p=20063","title":{"rendered":"2 Year-Old Human Lung Confocal Imaging Donor D022 SYP, SOX2, and TUBB3"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"20063\" class=\"elementor elementor-20063 elementor-bc-flex-widget\" data-elementor-post-type=\"post\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-44e4b776 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"44e4b776\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-13ef6d14\" data-id=\"13ef6d14\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-1a55e113 elementor-widget elementor-widget-theme-post-title elementor-page-title elementor-widget-heading\" data-id=\"1a55e113\" data-element_type=\"widget\" data-widget_type=\"theme-post-title.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h1 class=\"elementor-heading-title elementor-size-large\">2 Year-Old Human Lung Confocal Imaging Donor D022 SYP, SOX2, and TUBB3<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-7f3e486e elementor-section-boxed elementor-section-height-default elementor-section-height-default\" 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class=\"html5gallery-info\"><div class=\"html5gallery-title\">2 Year-Old Human Lung Immunofluorescence and Confocal Imaging Donor D022 Showing Expressions of SYP, SOX2, and TUBB3<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/03\/2018-03-15_LMH-16-D022-LLL-4B2.15_SYP_SOX2_TUBB3-02.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/03\/2018-03-15_LMH-16-D022-LLL-4B2.15_SYP_SOX2_TUBB3-02.jpg\" alt=\"2 Year-Old Human Lung Immunofluorescence and Confocal Imaging Donor D022 Showing Expressions of SYP, SOX2, and TUBB3\" data-description=\"60X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">2 Year-Old Human Lung Immunofluorescence and Confocal Imaging Donor D022 Showing Expressions of SYP, SOX2, and TUBB3<\/div><div class=\"html5gallery-description\">60X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/03\/2018-03-15_LMH-16-D022-LLL-4B2.15_SYP_SOX2_TUBB3-03.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/03\/2018-03-15_LMH-16-D022-LLL-4B2.15_SYP_SOX2_TUBB3-03.jpg\" alt=\"2 Year-Old Human Lung Immunofluorescence and Confocal Imaging Donor D022 Showing Expressions of SYP, SOX2, and TUBB3\" data-description=\"60X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">2 Year-Old Human Lung Immunofluorescence and Confocal Imaging Donor D022 Showing Expressions of SYP, SOX2, and TUBB3<\/div><div class=\"html5gallery-description\">60X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/03\/2018-03-15_LMH-16-D022-LLL-4B2.15_SYP_SOX2_TUBB3-04.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/03\/2018-03-15_LMH-16-D022-LLL-4B2.15_SYP_SOX2_TUBB3-04.jpg\" alt=\"2 Year-Old Human Lung Immunofluorescence and Confocal Imaging Donor D022 Showing Expressions of SYP, SOX2, and TUBB3\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">2 Year-Old Human Lung Immunofluorescence and Confocal Imaging Donor D022 Showing Expressions of SYP, SOX2, and TUBB3<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/03\/2018-03-15_LMH-16-D022-LLL-4B2.15_SYP_SOX2_TUBB3-05.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/03\/2018-03-15_LMH-16-D022-LLL-4B2.15_SYP_SOX2_TUBB3-05.jpg\" alt=\"2 Year-Old Human Lung Immunofluorescence and Confocal Imaging Donor D022 Showing Expressions of SYP, SOX2, and TUBB3\" data-description=\"60X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">2 Year-Old Human Lung Immunofluorescence and Confocal Imaging Donor D022 Showing Expressions of SYP, SOX2, and TUBB3<\/div><div class=\"html5gallery-description\">60X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/03\/2018-03-15_LMH-16-D022-LLL-4B2.15_SYP_SOX2_TUBB3-06.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/03\/2018-03-15_LMH-16-D022-LLL-4B2.15_SYP_SOX2_TUBB3-06.jpg\" alt=\"2 Year-Old Human Lung Immunofluorescence and Confocal Imaging Donor D022 Showing Expressions of SYP, SOX2, and TUBB3\" data-description=\"60X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">2 Year-Old Human Lung Immunofluorescence and Confocal Imaging Donor D022 Showing Expressions of SYP, SOX2, and TUBB3<\/div><div class=\"html5gallery-description\">60X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/03\/2018-03-15_LMH-16-D022-LLL-4B2.15_SYP_SOX2_TUBB3-07.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/03\/2018-03-15_LMH-16-D022-LLL-4B2.15_SYP_SOX2_TUBB3-07.jpg\" alt=\"2 Year-Old Human Lung Immunofluorescence and Confocal Imaging Donor D022 Showing Expressions of SYP, SOX2, and TUBB3\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">2 Year-Old Human Lung Immunofluorescence and Confocal Imaging Donor D022 Showing Expressions of SYP, SOX2, and TUBB3<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/03\/2018-03-15_LMH-16-D022-LLL-4B2.15_SYP_SOX2_TUBB3-08.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/03\/2018-03-15_LMH-16-D022-LLL-4B2.15_SYP_SOX2_TUBB3-08.jpg\" alt=\"2 Year-Old Human Lung Immunofluorescence and Confocal Imaging Donor D022 Showing Expressions of SYP, SOX2, and TUBB3\" data-description=\"60X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">2 Year-Old Human Lung Immunofluorescence and Confocal Imaging Donor D022 Showing Expressions of SYP, SOX2, and TUBB3<\/div><div class=\"html5gallery-description\">60X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/03\/2018-03-15_LMH-16-D022-LLL-4B2.15_SYP_SOX2_TUBB3-09.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/03\/2018-03-15_LMH-16-D022-LLL-4B2.15_SYP_SOX2_TUBB3-09.jpg\" alt=\"2 Year-Old Human Lung Immunofluorescence and Confocal Imaging Donor D022 Showing Expressions of SYP, SOX2, and TUBB3\" data-description=\"60X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">2 Year-Old Human Lung Immunofluorescence and Confocal Imaging Donor D022 Showing Expressions of SYP, SOX2, and TUBB3<\/div><div class=\"html5gallery-description\">60X<\/div><\/div><\/div><\/div><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-8f95da elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"8f95da\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-2b22a349\" data-id=\"2b22a349\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-20f7b0a1 elementor-widget elementor-widget-template\" data-id=\"20f7b0a1\" data-element_type=\"widget\" data-widget_type=\"template.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-template\">\n\t\t\t\t\t<div data-elementor-type=\"page\" data-elementor-id=\"28974\" class=\"elementor elementor-28974 elementor-bc-flex-widget\" data-elementor-post-type=\"elementor_library\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-4650a05 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"4650a05\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-951322a\" data-id=\"951322a\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-4f951ba\" data-id=\"4f951ba\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-0022067\" data-id=\"0022067\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-33e8076b elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"33e8076b\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-67558b96\" data-id=\"67558b96\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-7c7c80b2 elementor-widget elementor-widget-text-editor\" data-id=\"7c7c80b2\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"margin: 0px 0px 20px; font-family: 'PT Sans', Arial, Helvetica, sans-serif;\">Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from\u00a0the University of Rochester Medical Center<\/p><p style=\"margin: 0px 0px 20px; font-family: 'PT Sans', Arial, Helvetica, sans-serif;\"><a href=\"https:\/\/www.urmc.rochester.edu\/labs\/Pryhuber-Lab\/\" target=\"_blank\" rel=\"noopener noreferrer\"><img class=\"lazyload\" decoding=\"async\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27200%27%20height%3D%2748%27%20viewBox%3D%270%200%20200%2048%27%3E%3Crect%20width%3D%27200%27%20height%3D%2748%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2015\/07\/Pryhuber_Lab.jpg\" alt=\"Pryhuber_Lab\" width=\"200\" height=\"48\" \/><\/a><\/p><p style=\"margin: 0px 0px 20px; font-family: 'PT Sans', Arial, Helvetica, sans-serif; text-align: center;\"><span style=\"font-weight: bold;\">Immunofluorescence:\u00a0<\/span><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/RB-1461-P\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: bold;\">SYP<\/span><\/a><span style=\"font-weight: bold;\">,\u00a0<\/span><a href=\"https:\/\/www.scbt.com\/scbt\/product\/sox-2-antibody-e-4\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: bold;\">SOX2<\/span><\/a><span style=\"font-weight: bold;\">, and\u00a0<\/span><a href=\"http:\/\/www.biolegend.com\/purified-anti-tubulin-beta-3-tubb3-antibody-11580.html\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: bold;\">TUBB3<\/span><\/a><\/p><p style=\"margin: 0px 0px 20px; font-family: 'PT Sans', Arial, Helvetica, sans-serif;\"><span style=\"font-weight: bold;\">\u00a0<\/span><\/p><p style=\"margin: 0px 0px 20px; font-family: 'PT Sans', Arial, Helvetica, sans-serif;\"><span style=\"font-weight: bold;\">\u00a0<\/span><\/p><p style=\"margin: 0px 0px 20px; font-family: 'PT Sans', Arial, Helvetica, sans-serif;\"><span style=\"font-weight: bold;\">Purpose:\u00a0\u00a0<\/span>Stain slides of 7\u00b5m frozen sections of human lung from HTC for\u00a0<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/RB-1461-P\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: bold;\">SYP<\/span><\/a><span style=\"font-weight: bold;\">,\u00a0<\/span><a href=\"https:\/\/www.scbt.com\/scbt\/product\/sox-2-antibody-e-4\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: bold;\">SOX2<\/span><\/a><span style=\"font-weight: bold;\">, and\u00a0<\/span><a href=\"http:\/\/www.biolegend.com\/purified-anti-tubulin-beta-3-tubb3-antibody-11580.html\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: bold;\">TUBB3<\/span><\/a>\u00a0with antigen retrieval.<\/p><p style=\"margin: 0px 0px 20px; font-family: 'PT Sans', Arial, Helvetica, sans-serif;\"><span style=\"font-weight: bold;\">Day 1<\/span><\/p><ol style=\"font-family: 'PT Sans', Arial, Helvetica, sans-serif;\"><li>For Frozen tissue, rinse 2X in PBS, then 5 min in 4% PFA\/PBS, then rinse 1X in PBS.<\/li><li>Briefly equilibrate slides in Antigen Retrieval Buffer.<\/li><li>Antigen retrieval, pH 6.0 (times will vary according to microwave).<\/li><li>10 mM sodium citrate, pH 6.0, and heat in a microwave at 96<sup>o<\/sup>C.\u00a0*We usually do a series of three runs (6-7 minutes each run) to equal the time\/temp because of evaporation (refill coplin jars with dH<sub>2<\/sub>O).<\/li><li>Microwave according to instructions on microwave.<\/li><li>Cool on countertop, 15 min.<\/li><li>Rinse with dH<sub>2<\/sub>O<\/li><li>1X PBS, 5 min.<\/li><li>Block in 4% Donkey serum\/PBS-T, 2 hours at RT.<\/li><li>For Rabbit anti-<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/RB-1461-P\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: bold;\">SYP<\/span><\/a>\u00a0(<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/RB-1461-P\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: bold;\">RB-1461-P<\/span><\/a>, lot# 1461P1712D, Thermo Fisher) dilute 1:100, for mouse anti-<a href=\"https:\/\/www.scbt.com\/scbt\/product\/sox-2-antibody-e-4\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: bold;\">SOX2<\/span><\/a>\u00a0(<a href=\"https:\/\/www.scbt.com\/scbt\/product\/sox-2-antibody-e-4\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: bold;\">SC-365823<\/span><\/a>, Santa Cruz, Lot# C0317) dilute 1:100, for mouse anti-<a href=\"http:\/\/www.biolegend.com\/purified-anti-tubulin-beta-3-tubb3-antibody-11580.html\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: bold;\">TUBB3<\/span><\/a>\u00a0(anti-<a href=\"http:\/\/www.biolegend.com\/purified-anti-tubulin-beta-3-tubb3-antibody-11580.html\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: bold;\">tubulin \u03b2 3<\/span><\/a>, cat. # 801201, Biolegend)\u00a0dilute 1:200 in blocking buffer. Spin down in \u00b5fuge for 20 minutes and incubate on tissue overnight @ 4<sup>o<\/sup>C.<\/li><\/ol><p style=\"margin: 0px 0px 20px; font-family: 'PT Sans', Arial, Helvetica, sans-serif;\"><span style=\"font-weight: bold;\">Day 2<\/span><\/p><ol style=\"font-family: 'PT Sans', Arial, Helvetica, sans-serif;\"><li>Rinse slides in PBS-T 3X, 5 min.<\/li><li>Apply secondary antibody , Donkey Alexa Fluor 488 anti-rabbit IgG (<a href=\"https:\/\/www.lifetechnologies.com\/order\/catalog\/product\/A21206?ICID=search-a21206\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: bold;\">A21206<\/span><\/a>, Lot# 1608521, for anti-<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/RB-1461-P\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: bold;\">SYP<\/span><\/a>) at 1:200, Goat Alexa Fluor 568 anti-mouse IgG1 (<a href=\"https:\/\/www.thermofisher.com\/antibody\/product\/Goat-anti-Mouse-IgG1-Cross-Adsorbed-Secondary-Antibody-Polyclonal\/A-21124\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: bold;\">A21124<\/span><\/a>, Lot# 1600878, for anti-<a href=\"https:\/\/www.scbt.com\/scbt\/product\/sox-2-antibody-e-4\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: bold;\">SOX2<\/span><\/a>) at 1:200, Donkey Alexa Fluor 633 anti-mouse IgG2A\u00a0<a href=\"https:\/\/www.thermofisher.com\/order\/genome-database\/antibody\/Mouse-IgG2a-Secondary-Antibody-Polyclonal\/A-21136\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: bold;\">A21136<\/span><\/a>, Lot# 1345055,\u00a0<a href=\"http:\/\/www.sigmaaldrich.com\/catalog\/product\/sigma\/a5228?lang=en%C2%AEion=US\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: bold;\">TUBB3<\/span>)<\/a>\u00a0at 1:200, in blocking buffer. Spin down in \u00b5fuge for 20 min, apply to tissue and incubated at room temperature for 1 hour.<\/li><li>Rinse in PBS-T 3X, 5 min.<\/li><li>Dilute DAPI 1:2000 and apply to slides for 10 min.<\/li><li>Wash in PBS-T 3X, 5 min.<\/li><li>Rinse slides in 0.1M PB or TB, 3X, 5 min.<\/li><li>Add 1 drop of Prolong Gold anti-fade mounting medium (P36930).<\/li><li>Coverslip with Gold Seal Coverslip (Cat# 3422 Electron Microscopy Sciences, 22 X 22 mm).<\/li><li>Allow Prolong Gold to cure overnight at room temp in light sealed box.<\/li><\/ol><ol style=\"font-family: 'PT Sans', Arial, Helvetica, sans-serif;\" start=\"10\"><li>Store slides in light sealed box @ 4<sup>o<\/sup>C<\/li><\/ol><p style=\"margin: 0px 0px 20px; font-family: 'PT Sans', Arial, Helvetica, sans-serif;\"><span style=\"font-weight: bold;\">\u00a0<\/span><\/p><p style=\"margin: 0px 0px 20px; font-family: 'PT Sans', Arial, Helvetica, sans-serif;\"><span style=\"font-weight: bold;\">\u00a0<\/span><\/p><p style=\"margin: 0px 0px 20px; font-family: 'PT Sans', Arial, Helvetica, sans-serif;\"><span style=\"font-weight: bold;\">Tissue Used:<br \/><\/span><span style=\"font-weight: bold; letter-spacing: 0px;\">LMH-16-D022-LLL-4B2.15<br \/><\/span><span style=\"font-weight: bold; letter-spacing: 0px;\">Gender: Male<br \/><\/span><span style=\"font-weight: bold; letter-spacing: 0px;\">Weight: 1.06g<\/span><\/p><p style=\"margin: 0px 0px 20px; font-family: 'PT Sans', Arial, Helvetica, sans-serif;\"><span style=\"font-weight: bold;\">\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/span><\/p><p style=\"margin: 0px 0px 20px; font-family: 'PT Sans', Arial, Helvetica, sans-serif;\"><span style=\"font-weight: bold;\">Appendix<br \/><\/span><span style=\"font-weight: bold; letter-spacing: 0px;\">Antigen Retrieval Solution<\/span><\/p><p style=\"margin: 0px 0px 20px; font-family: 'PT Sans', Arial, Helvetica, sans-serif;\">18ml Solution A- 0.1M Citric Acid (Sigma, C1909) pH 2.5<\/p><p style=\"margin: 0px 0px 20px; font-family: 'PT Sans', Arial, Helvetica, sans-serif;\">82ml Reagent B- 0.1M Sodium Citrate (Sigma, S4641 ) pH 8.2<\/p><p style=\"margin: 0px 0px 20px; font-family: 'PT Sans', Arial, Helvetica, sans-serif;\">1L dH<sub>2<\/sub>O<\/p><p style=\"margin: 0px 0px 20px; font-family: 'PT Sans', Arial, Helvetica, sans-serif;\">pH 6.0<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-acdb235 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"acdb235\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-37274723\" data-id=\"37274723\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-73caa553 elementor-widget elementor-widget-facebook-comments\" data-id=\"73caa553\" data-element_type=\"widget\" data-widget_type=\"facebook-comments.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-facebook-widget fb-comments\" data-href=\"https:\/\/research.cchmc.org\/lungimage?p=20063\" data-width=\"100%\" data-numposts=\"10\" data-order-by=\"social\" style=\"min-height: 1px\"><\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-19da06e\" data-id=\"19da06e\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-617e4308 elementor-widget elementor-widget-sidebar\" data-id=\"617e4308\" data-element_type=\"widget\" data-widget_type=\"sidebar.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div id=\"search-86\" class=\"widget widget_search\">\t\t<form role=\"search\" class=\"searchform fusion-search-form  fusion-search-form-classic\" method=\"get\" action=\"https:\/\/research.cchmc.org\/lungimage\/\">\n\t\t\t<div class=\"fusion-search-form-content\">\n\n\t\t\t\t\n\t\t\t\t<div class=\"fusion-search-field search-field\">\n\t\t\t\t\t<label><span class=\"screen-reader-text\">Search for:<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<input type=\"search\" value=\"\" name=\"s\" class=\"s\" placeholder=\"Search...\" required aria-required=\"true\" aria-label=\"Search...\"\/>\n\t\t\t\t\t\t\t\t\t\t\t<\/label>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"fusion-search-button search-button\">\n\t\t\t\t\t<input type=\"submit\" class=\"fusion-search-submit searchsubmit\" aria-label=\"Search\" value=\"&#xf002;\" \/>\n\t\t\t\t\t\t\t\t\t<\/div>\n\n\t\t\t\t\n\t\t\t<\/div>\n\n\n\t\t\t\n\t\t<\/form>\n\t\t<\/div><div id=\"text-74\" class=\"widget widget_text\"><div class=\"heading\"><h4 class=\"widget-title\">Related Experiments<\/h4><\/div>\t\t\t<div class=\"textwidget\"><div class=\"wondercarousellightbox_options\" data-skinsfoldername=\"\"  data-jsfolder=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/plugins\/wonderplugin-carousel\/engine\/\" style=\"display:none;\"><\/div>\r\n<div class=\"wonderplugincarousel-container\" id=\"wonderplugincarousel-container-637\"><div class=\"wonderplugincarousel\" id=\"wonderplugincarousel-637\" data-carouselid=\"637\" data-width=\"200\" data-height=\"200\" data-skin=\"vertical\" data-showimgtitle=\"false\" data-sameheight=\"false\" data-sameheightresponsive=\"false\" data-fullwidth=\"false\" data-centerimage=\"false\" data-fitimage=\"false\" data-fitcenterimage=\"false\" data-fixaspectratio=\"false\" data-autoplay=\"true\" data-random=\"false\" data-circular=\"false\" data-pauseonmouseover=\"true\" data-continuous=\"false\" data-responsive=\"true\" data-showhoveroverlay=\"true\" data-showhoveroverlayalways=\"false\" data-hidehoveroverlayontouch=\"false\" data-lightboxresponsive=\"true\" data-lightboxshownavigation=\"false\" data-lightboxnogroup=\"false\" data-lightboxshowtitle=\"true\" data-lightboxshowdescription=\"false\" data-lightboxaddsocialmedia=\"false\" data-usescreenquery=\"false\" data-donotinit=\"false\" data-addinitscript=\"false\" data-doshortcodeontext=\"false\" data-lightboxshowsocial=\"false\" data-lightboxshowfacebook=\"true\" data-lightboxshowtwitter=\"true\" data-lightboxshowpinterest=\"true\" data-lightboxsocialrotateeffect=\"true\" data-donotcircularforless=\"false\" data-hidecontainerbeforeloaded=\"false\" data-hidecontaineroninit=\"false\" data-lightboximagekeepratio=\"true\" data-showplayvideo=\"true\" data-triggerresize=\"false\" data-lightboxfullscreenmode=\"false\" data-lightboxcloseonoverlay=\"true\" data-lightboxvideohidecontrols=\"false\" data-lightboxautoslide=\"false\" data-lightboxshowtimer=\"true\" data-lightboxshowplaybutton=\"true\" data-lightboxalwaysshownavarrows=\"false\" data-lightboxshowtitleprefix=\"true\" data-spacing=\"12\" data-rownumber=\"1\" data-visibleitems=\"6\" data-arrowstyle=\"always\" data-arrowimage=\"arrows-32-32-4.png\" data-arrowwidth=\"32\" data-arrowheight=\"32\" data-navstyle=\"none\" data-navimage=\"bullet-24-24-0.png\" data-navwidth=\"24\" data-navheight=\"24\" data-navspacing=\"4\" data-hoveroverlayimage=\"hoveroverlay-64-64-4.png\" data-lightboxthumbwidth=\"90\" data-lightboxthumbheight=\"60\" data-lightboxthumbtopmargin=\"12\" data-lightboxthumbbottommargin=\"4\" data-lightboxbarheight=\"64\" data-lightboxtitlebottomcss=\"{color:#002e9b; font-size:14px; font-family:Armata,sans-serif,Arial; overflow:hidden; text-align:center;}\" data-lightboxdescriptionbottomcss=\"{color:#333; font-size:12px; font-family:Arial,Helvetica,sans-serif; overflow:hidden; text-align:left; margin:4px 0px 0px; padding: 0px;}\" data-continuousduration=\"2500\" data-autoplaydir=\"left\" data-scrollmode=\"page\" data-interval=\"3000\" data-transitionduration=\"1000\" data-lightboxtitlestyle=\"bottom\" data-lightboximagepercentage=\"75\" data-lightboxdefaultvideovolume=\"1\" data-lightboxoverlaybgcolor=\"#000\" data-lightboxoverlayopacity=\"0.9\" data-lightboxbgcolor=\"#fff\" data-lightboxtitleprefix=\"%NUM \/ %TOTAL\" data-lightboxtitleinsidecss=\"color:#fff; font-size:16px; font-family:Arial,Helvetica,sans-serif; overflow:hidden; text-align:left;\" data-lightboxdescriptioninsidecss=\"color:#fff; font-size:12px; font-family:Arial,Helvetica,sans-serif; overflow:hidden; text-align:left; margin:4px 0px 0px; padding: 0px;\" data-playvideoimage=\"playvideo-64-64-0.png\" data-playvideoimagepos=\"center\" data-imgtitle=\"title\" data-circularlimit=\"3\" data-lightboxsocialposition=\"position:absolute;top:100%;right:0;\" data-lightboxsocialpositionsmallscreen=\"position:absolute;top:100%;right:0;left:0;\" data-lightboxsocialdirection=\"horizontal\" data-lightboxsocialbuttonsize=\"32\" data-lightboxsocialbuttonfontsize=\"18\" data-sameheightmediumscreen=\"769\" data-sameheightmediumheight=\"200\" data-sameheightsmallscreen=\"415\" data-sameheightsmallheight=\"150\" data-triggerresizedelay=\"100\" data-lightboxslideinterval=\"5000\" data-lightboxtimerposition=\"bottom\" data-lightboxtimercolor=\"#dc572e\" data-lightboxtimeropacity=\"1\" data-lightboxbordersize=\"8\" data-lightboxborderradius=\"0\" data-screenquery='{ \"mobile\": { \"screenwidth\": 600, \"visibleitems\": 1 } }' data-jsfolder=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/plugins\/wonderplugin-carousel\/engine\/\" style=\"display:none;position:relative;margin:0 auto;width:100%;max-width:200px;\" ><div class=\"amazingcarousel-list-container\" style=\"overflow:hidden;\"><ul class=\"amazingcarousel-list\"><li class=\"amazingcarousel-item\"><div class=\"amazingcarousel-item-container\"><div class=\"amazingcarousel-image\">\n\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=20378\" target=\"_blank\"><img decoding=\"async\" class=\"lazyload amazingcarousel-image-img\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27300%27%20height%3D%27300%27%20viewBox%3D%270%200%20300%20300%27%3E%3Crect%20width%3D%27300%27%20height%3D%27300%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/03\/2018-03-21_LMH-16-D086-LLL-8A3.22_SYP_SOX2_TUBB3-01-2.jpg\" alt=\"8 Month-Old Human Lung Confocal Imaging Donor D086 SYP, SOX2, and TUBB3\" data-description=\"\" \/><\/a>\n\t<div class=\"amazingcarousel-text\">\n\t\t<div class=\"amazingcarousel-text-bg\"><\/div>\n\t\t<div class=\"amazingcarousel-title\">8 Month-Old Human Lung Confocal Imaging Donor D086 SYP, SOX2, and TUBB3<\/div>\n\t<\/div>\n<\/div><\/div><\/li><li class=\"amazingcarousel-item\"><div class=\"amazingcarousel-item-container\"><div class=\"amazingcarousel-image\">\n\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=20462\" target=\"_blank\"><img decoding=\"async\" class=\"lazyload amazingcarousel-image-img\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27300%27%20height%3D%27300%27%20viewBox%3D%270%200%20300%20300%27%3E%3Crect%20width%3D%27300%27%20height%3D%27300%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/03\/2018-03-22_LMH-16-D083-LLL-8A3.7_SYP_SOX2_TUBB3-03-1.jpg\" alt=\"13 Month-Old Human Lung Confocal Imaging Donor D083 SYP, SOX2, and TUBB3\" data-description=\"\" \/><\/a>\n\t<div class=\"amazingcarousel-text\">\n\t\t<div class=\"amazingcarousel-text-bg\"><\/div>\n\t\t<div class=\"amazingcarousel-title\">13 Month-Old Human Lung Confocal Imaging Donor D083 SYP, SOX2, and TUBB3<\/div>\n\t<\/div>\n<\/div><\/div><\/li><li class=\"amazingcarousel-item\"><div class=\"amazingcarousel-item-container\"><div class=\"amazingcarousel-image\">\n\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=20063\" target=\"_blank\"><img decoding=\"async\" class=\"lazyload amazingcarousel-image-img\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27300%27%20height%3D%27300%27%20viewBox%3D%270%200%20300%20300%27%3E%3Crect%20width%3D%27300%27%20height%3D%27300%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/03\/2018-03-15_LMH-16-D022-LLL-4B2.15_SYP_SOX2_TUBB3-09-2.jpg\" alt=\"2 Year-Old Human Lung Confocal Imaging Donor D022 SYP, SOX2, and TUBB3\" data-description=\"\" \/><\/a>\n\t<div class=\"amazingcarousel-text\">\n\t\t<div class=\"amazingcarousel-text-bg\"><\/div>\n\t\t<div class=\"amazingcarousel-title\">2 Year-Old Human Lung Confocal Imaging Donor D022 SYP, SOX2, and TUBB3<\/div>\n\t<\/div>\n<\/div><\/div><\/li><li class=\"amazingcarousel-item\"><div class=\"amazingcarousel-item-container\"><div class=\"amazingcarousel-image\">\n\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=20090\" target=\"_blank\"><img decoding=\"async\" class=\"lazyload amazingcarousel-image-img\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27300%27%20height%3D%27300%27%20viewBox%3D%270%200%20300%20300%27%3E%3Crect%20width%3D%27300%27%20height%3D%27300%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/03\/2018-03-15_LMH-16-D032-LLL-8B2.30_SYP_SOX2_TUBB3-19-1.jpg\" alt=\"3 Year-Old Human Lung Confocal Imaging Donor D032 SYP, SOX2, and TUBB3\" data-description=\"\" \/><\/a>\n\t<div class=\"amazingcarousel-text\">\n\t\t<div class=\"amazingcarousel-text-bg\"><\/div>\n\t\t<div class=\"amazingcarousel-title\">3 Year-Old Human Lung Confocal Imaging Donor D032 SYP, SOX2, and TUBB3<\/div>\n\t<\/div>\n<\/div><\/div><\/li><li class=\"amazingcarousel-item\"><div class=\"amazingcarousel-item-container\"><div class=\"amazingcarousel-image\">\n\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=20367\" target=\"_blank\"><img decoding=\"async\" class=\"lazyload amazingcarousel-image-img\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27300%27%20height%3D%27300%27%20viewBox%3D%270%200%20300%20300%27%3E%3Crect%20width%3D%27300%27%20height%3D%27300%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/03\/2018-03-20_LMH-16-D015-LLL-6C4.17_SYP_SOX2_TUBB3-02-1.jpg\" alt=\"3 Year-Old Human Lung Confocal Imaging Donor D015 SYP, SOX2, and TUBB3\" data-description=\"\" \/><\/a>\n\t<div class=\"amazingcarousel-text\">\n\t\t<div class=\"amazingcarousel-text-bg\"><\/div>\n\t\t<div class=\"amazingcarousel-title\">3 Year-Old Human Lung Confocal Imaging Donor D015 SYP, SOX2, and TUBB3<\/div>\n\t<\/div>\n<\/div><\/div><\/li><li class=\"amazingcarousel-item\"><div class=\"amazingcarousel-item-container\"><div class=\"amazingcarousel-image\">\n\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=20397\" target=\"_blank\"><img decoding=\"async\" class=\"lazyload amazingcarousel-image-img\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27300%27%20height%3D%27300%27%20viewBox%3D%270%200%20300%20300%27%3E%3Crect%20width%3D%27300%27%20height%3D%27300%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/03\/2018-03-19_LMH-16-D039-LLL-6A3.10_SYP_SOX2_TUBB3-03-1.jpg\" alt=\"3 Year-Old Human Lung Confocal Imaging Donor D039 SYP, SOX2, and TUBB3\" data-description=\"\" \/><\/a>\n\t<div class=\"amazingcarousel-text\">\n\t\t<div class=\"amazingcarousel-text-bg\"><\/div>\n\t\t<div class=\"amazingcarousel-title\">3 Year-Old Human Lung Confocal Imaging Donor D039 SYP, SOX2, and TUBB3<\/div>\n\t<\/div>\n<\/div><\/div><\/li><li class=\"amazingcarousel-item\"><div class=\"amazingcarousel-item-container\"><div class=\"amazingcarousel-image\">\n\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=20435\" target=\"_blank\"><img decoding=\"async\" class=\"lazyload amazingcarousel-image-img\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27300%27%20height%3D%27300%27%20viewBox%3D%270%200%20300%20300%27%3E%3Crect%20width%3D%27300%27%20height%3D%27300%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/03\/2018-03-19_LMH-16-D053-LLL-6B2.17_SYP_SOX2_TUBB3-08-1.jpg\" alt=\"3 Year-Old Human Lung Confocal Imaging Donor D053 SYP, SOX2, and TUBB3\" data-description=\"\" \/><\/a>\n\t<div class=\"amazingcarousel-text\">\n\t\t<div class=\"amazingcarousel-text-bg\"><\/div>\n\t\t<div class=\"amazingcarousel-title\">3 Year-Old Human Lung Confocal Imaging Donor D053 SYP, SOX2, and TUBB3<\/div>\n\t<\/div>\n<\/div><\/div><\/li><\/ul><div class=\"amazingcarousel-prev\"><\/div><div class=\"amazingcarousel-next\"><\/div><\/div><div class=\"amazingcarousel-nav\"><\/div><\/div><\/div><script>function wondercarousel_637_appendcss(csscode) {var head=document.head || document.getElementsByTagName(\"head\")[0];var style=document.createElement(\"style\");head.appendChild(style);style.type=\"text\/css\";if (style.styleSheet){style.styleSheet.cssText=csscode;} else 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\tmargin-bottom: -36px; }  #wonderplugincarousel-637 .amazingcarousel-nav { \tposition: absolute; \ttop: 0%; \tleft: 100%; }  #wonderplugincarousel-637 .amazingcarousel-bullet-wrapper { \tmargin: 16px; } .amazingcarousel-socialmedia-button { display: inline-block; margin: 4px; }.amazingcarousel-socialmedia-button a { box-shadow: none; }.amazingcarousel-socialmedia-icon { display:table-cell; width:32px; height:32px; font-size:18px; border-radius:50%; color:#fff; vertical-align:middle; text-align:center; cursor:pointer; padding:0;}.amazingcarousel-socialmedia-rotate { transition: transform .4s ease-in; } .amazingcarousel-socialmedia-rotate:hover { transform: rotate(360deg); }\");<\/script>\n<\/div>\n\t\t<\/div>\n\t\t<div id=\"recent-posts-85\" class=\"widget widget_recent_entries\">\n\t\t<div class=\"heading\"><h4 class=\"widget-title\">Recent Experiments<\/h4><\/div>\n\t\t<ul>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=32066\">4 Year-Old Human Lung KRT14, MUC5B, and TUBA4A Confocal Imaging<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31223\">Alcian Blue Staining from PBR 3653 Patient with Cystic Fibrosis<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31202\">H&#038;E Staining from PBR 3383 Patient with Cystic Fibrosis<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31199\">H&#038;E Staining from PBR 3653 Patient with Cystic Fibrosis<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31196\">H&#038;E Staining of 31 Year-Old Human Donor 10<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t<\/ul>\n\n\t\t<\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from\u00a0the University of Rochester Medical Center Immunofluorescence:\u00a0SYP,\u00a0SOX2, and\u00a0TUBB3 \u00a0 \u00a0 Purpose:\u00a0\u00a0Stain slides of 7\u00b5m frozen sections of human lung from HTC for\u00a0SYP,\u00a0SOX2, and\u00a0TUBB3\u00a0with antigen retrieval. Day 1 For Frozen tissue, rinse 2X in PBS, then 5 min in 4% PFA\/PBS, then rinse 1X in PBS. Briefly equilibrate [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":20064,"comment_status":"closed","ping_status":"open","sticky":false,"template":"elementor_header_footer","format":"standard","meta":{"footnotes":""},"categories":[165,275],"tags":[31,198,109],"class_list":["post-20063","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-confocal_2y0","category-homo-sapiens-confocal","tag-sox2","tag-syp","tag-tubb3"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v25.6 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>2 Year-Old Human Lung Confocal Imaging Donor D022 SYP, SOX2, and TUBB3 -<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/research.cchmc.org\/lungimage\/?p=20063\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"2 Year-Old Human Lung Confocal Imaging Donor D022 SYP, SOX2, and TUBB3 -\" \/>\n<meta property=\"og:description\" content=\"Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from\u00a0the University of Rochester Medical Center Immunofluorescence:\u00a0SYP,\u00a0SOX2, and\u00a0TUBB3 \u00a0 \u00a0 Purpose:\u00a0\u00a0Stain slides of 7\u00b5m frozen sections of human lung from HTC for\u00a0SYP,\u00a0SOX2, and\u00a0TUBB3\u00a0with antigen retrieval. Day 1 For Frozen tissue, rinse 2X in PBS, then 5 min in 4% PFA\/PBS, then rinse 1X in PBS. Briefly equilibrate [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/research.cchmc.org\/lungimage\/?p=20063\" \/>\n<meta property=\"article:published_time\" content=\"2018-03-16T17:27:11+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2018-11-12T18:27:33+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/03\/2018-03-15_LMH-16-D022-LLL-4B2.15_SYP_SOX2_TUBB3-09-2.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"300\" \/>\n\t<meta property=\"og:image:height\" content=\"300\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"kitc5i\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"kitc5i\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"2 minutes\" 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