{"id":2022,"date":"2015-04-03T00:13:22","date_gmt":"2015-04-03T00:13:22","guid":{"rendered":"https:\/\/research.cchmc.org\/lungimage\/?p=2022"},"modified":"2018-08-17T22:43:14","modified_gmt":"2018-08-17T22:43:14","slug":"e18-5-c57bl6-nkx2-1-scgb1a1-and-tuba1a-confocal-imaging","status":"publish","type":"post","link":"https:\/\/research.cchmc.org\/lungimage\/?p=2022","title":{"rendered":"E18.5 C57BL6 NKX2.1, SCGB1A1, and TUBA1A Confocal Imaging"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"2022\" class=\"elementor elementor-2022 elementor-bc-flex-widget\" data-elementor-post-type=\"post\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-5ea18580 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"5ea18580\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-ba08fb4\" data-id=\"ba08fb4\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-31ee792 elementor-widget elementor-widget-theme-post-title elementor-page-title elementor-widget-heading\" data-id=\"31ee792\" data-element_type=\"widget\" data-widget_type=\"theme-post-title.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h1 class=\"elementor-heading-title elementor-size-large\">E18.5 C57BL6 NKX2.1, SCGB1A1, and TUBA1A Confocal Imaging<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-79498b0 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"79498b0\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-61b26cc7\" data-id=\"61b26cc7\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-509c656b elementor-widget elementor-widget-shortcode\" data-id=\"509c656b\" data-element_type=\"widget\" data-widget_type=\"shortcode.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-shortcode\"><div class=\"wonderplugingallery-container\" id=\"wonderplugingallery-container-6\" style=\"max-width:550px;margin:0 auto;\"><div class=\"wonderplugingallery\" id=\"wonderplugingallery-6\" data-galleryid=\"6\" data-width=\"550\" data-height=\"550\" data-skin=\"light\" data-autoslide=\"false\" data-autoplayvideo=\"false\" data-html5player=\"true\" data-responsive=\"true\" 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data-lightboxtitlecss=\"{color:#333333; font:bold 12px Arial,Helvetica,sans-serif; overflow:hidden; white-space:normal; text-align:Center; line-height:18px;}\" data-lightboxdescriptioncss=\"{color:#333333; font:normal 12px Arial,Helvetica,sans-serif; overflow:hidden; white-space:normal; text-align:Center; line-height:14px;}\" data-titlecss=\"{color:#ffffff; font-size:14px; font-family:Armata, sans-serif, Arial; overflow:hidden; white-space:normal; text-align:Center; padding:10px 0px 10px 10px;  background:rgb(102, 102, 102) transparent; background:rgba(102, 102, 102, 0.6);}\" data-descriptioncss=\"{color:#ffffff; font-size:12px; font-family:Armata, sans-serif, Arial; overflow:hidden; white-space:normal; text-align:Center; padding:0px 0px 10px 10px;  background:rgb(102, 102, 102) transparent; background:rgba(102, 102, 102, 0.6);}\" data-jsfolder=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/plugins\/wonderplugin-gallery\/engine\/\" style=\"display:none;\" ><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2015\/02\/2014-012-018_C57Bl6_E18.5_LMM.14.27.6.63_NKX2.1_SCGB1A1_TUBA1A-011.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2015\/02\/2014-012-018_C57Bl6_E18.5_LMM.14.27.6.63_NKX2.1_SCGB1A1_TUBA1A-011.jpg\" alt=\"E18.5 Confocal Imaging Showing Protein Expression of Nkx2.1, Scgb1a1, and Tuba1a Genes\" data-description=\"20X Tile Scan\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">E18.5 Confocal Imaging Showing Protein Expression of Nkx2.1, Scgb1a1, and Tuba1a Genes<\/div><div class=\"html5gallery-description\">20X Tile Scan<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2015\/02\/2014-012-018_C57Bl6_E18.5_LMM.14.27.6.63_NKX2.1_SCGB1A1_TUBA1A-021.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2015\/02\/2014-012-018_C57Bl6_E18.5_LMM.14.27.6.63_NKX2.1_SCGB1A1_TUBA1A-021.jpg\" alt=\"E18.5 Confocal Imaging Showing Protein Expression of Nkx2.1, Scgb1a1, and Tuba1a Genes\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">E18.5 Confocal Imaging Showing Protein Expression of Nkx2.1, Scgb1a1, and Tuba1a Genes<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2015\/02\/2014-012-018_C57Bl6_E18.5_LMM.14.27.6.63_NKX2.1_SCGB1A1_TUBA1A-031.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2015\/02\/2014-012-018_C57Bl6_E18.5_LMM.14.27.6.63_NKX2.1_SCGB1A1_TUBA1A-031.jpg\" alt=\"E18.5 Confocal Imaging Showing Protein Expression of Nkx2.1, Scgb1a1, and Tuba1a Genes\" data-description=\"60X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">E18.5 Confocal Imaging Showing Protein Expression of Nkx2.1, Scgb1a1, and Tuba1a Genes<\/div><div class=\"html5gallery-description\">60X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2015\/02\/2014-012-018_C57Bl6_E18.5_LMM.14.27.6.63_NKX2.1_SCGB1A1_TUBA1A-041.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2015\/02\/2014-012-018_C57Bl6_E18.5_LMM.14.27.6.63_NKX2.1_SCGB1A1_TUBA1A-041.jpg\" alt=\"E18.5 Confocal Imaging Showing Protein Expression of Nkx2.1, Scgb1a1, and Tuba1a Genes\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">E18.5 Confocal Imaging Showing Protein Expression of Nkx2.1, Scgb1a1, and Tuba1a Genes<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2015\/02\/2014-012-018_C57Bl6_E18.5_LMM.14.27.6.63_NKX2.1_SCGB1A1_TUBA1A-051.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2015\/02\/2014-012-018_C57Bl6_E18.5_LMM.14.27.6.63_NKX2.1_SCGB1A1_TUBA1A-051.jpg\" alt=\"E18.5 Confocal Imaging Showing Protein Expression of Nkx2.1, Scgb1a1, and Tuba1a Genes\" data-description=\"60X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">E18.5 Confocal Imaging Showing Protein Expression of Nkx2.1, Scgb1a1, and Tuba1a Genes<\/div><div class=\"html5gallery-description\">60X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2015\/02\/2014-012-018_C57Bl6_E18.5_LMM.14.27.6.63_NKX2.1_SCGB1A1_TUBA1A-081.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2015\/02\/2014-012-018_C57Bl6_E18.5_LMM.14.27.6.63_NKX2.1_SCGB1A1_TUBA1A-081.jpg\" alt=\"E18.5 Confocal Imaging Showing Protein Expression of Nkx2.1, Scgb1a1, and Tuba1a Genes\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">E18.5 Confocal Imaging Showing Protein Expression of Nkx2.1, Scgb1a1, and Tuba1a Genes<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2015\/02\/2014-012-018_C57Bl6_E18.5_LMM.14.27.6.63_NKX2.1_SCGB1A1_TUBA1A-091.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2015\/02\/2014-012-018_C57Bl6_E18.5_LMM.14.27.6.63_NKX2.1_SCGB1A1_TUBA1A-091.jpg\" alt=\"E18.5 Confocal Imaging Showing Protein Expression of Nkx2.1, Scgb1a1, and Tuba1a Genes\" data-description=\"60X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">E18.5 Confocal Imaging Showing Protein Expression of Nkx2.1, Scgb1a1, and Tuba1a Genes<\/div><div class=\"html5gallery-description\">60X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2015\/02\/2014-012-018_C57Bl6_E18.5_LMM.14.27.6.63_NKX2.1_SCGB1A1_TUBA1A-061.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2015\/02\/2014-012-018_C57Bl6_E18.5_LMM.14.27.6.63_NKX2.1_SCGB1A1_TUBA1A-061.jpg\" alt=\"E18.5 Confocal Imaging Showing Protein Expression of Nkx2.1, Scgb1a1, and Tuba1a Genes\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">E18.5 Confocal Imaging Showing Protein Expression of Nkx2.1, Scgb1a1, and Tuba1a Genes<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2015\/02\/2014-012-018_C57Bl6_E18.5_LMM.14.27.6.63_NKX2.1_SCGB1A1_TUBA1A-071.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2015\/02\/2014-012-018_C57Bl6_E18.5_LMM.14.27.6.63_NKX2.1_SCGB1A1_TUBA1A-071.jpg\" alt=\"E18.5 Confocal Imaging Showing Protein Expression of Nkx2.1, Scgb1a1, and Tuba1a Genes\" data-description=\"60X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">E18.5 Confocal Imaging Showing Protein Expression of Nkx2.1, Scgb1a1, and Tuba1a Genes<\/div><div class=\"html5gallery-description\">60X<\/div><\/div><\/div><\/div><\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-3257bc7 elementor-widget elementor-widget-template\" data-id=\"3257bc7\" data-element_type=\"widget\" data-widget_type=\"template.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-template\">\n\t\t\t\t\t<div data-elementor-type=\"page\" data-elementor-id=\"24472\" class=\"elementor elementor-24472 elementor-bc-flex-widget\" data-elementor-post-type=\"elementor_library\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-4650a05 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"4650a05\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-951322a\" data-id=\"951322a\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-4f951ba\" data-id=\"4f951ba\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-0022067\" data-id=\"0022067\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-4a8d0ace elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"4a8d0ace\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-7c98d269\" data-id=\"7c98d269\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-738fb8a5 elementor-widget elementor-widget-text-editor\" data-id=\"738fb8a5\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"text-align: center;\"><strong>Immunofluorescence-\u00a0NKX2.1, SCGB1A1, \u00a0TUBA1A<\/strong><\/p><p><strong style=\"letter-spacing: 0px;\">Purpose: <\/strong><span style=\"letter-spacing: 0px;\">Stain slides of 7\u00b5m frozen sections of C57BL\/6J E18.5 NKX2.1, SCGB1A1<\/span><strong style=\"letter-spacing: 0px;\">, <\/strong><span style=\"letter-spacing: 0px;\">and <\/span><a style=\"letter-spacing: 0px;\" href=\"http:\/\/www.sigmaaldrich.com\/catalog\/product\/sigma\/t7451?lang=en&amp;region=US\" target=\"_Blank\"><strong>TUBA1A<\/strong><\/a><span style=\"letter-spacing: 0px;\"> with antigen retrieval.<\/span><strong>\u00a0<\/strong><\/p><p><strong>\u00a0<\/strong><\/p><p><strong>Day 1<\/strong><\/p><ol><li>For Frozen tissue, rinse 2X in PBS, then 5 min in 4% PFA\/PBS, then rinse 1X in PBS.<\/li><li>Antigen retrieval, pH 6.0 (times will vary according to microwave). Briefly equilibrate slides in Antigen Retrieval Buffer.<\/li><li>10 mM sodium citrate, pH 6.0, and heat in a microwave at 96<sup>o<\/sup>C.\u00a0*We usually do a series of three runs (6-7 minutes each run) to equal the time\/temp because of evaporation (refill coplin jars with dH<sub>2<\/sub>O).<\/li><li>Microwave according to instructions on microwave.<\/li><li>Cool on counter top, 15 min.<\/li><li>Rinse with dH<sub>2<\/sub>O.<\/li><li>1X PBS, 5 min.<\/li><li>Block in 4% Goat serum\/PBS-T, 2 hours at RT.<\/li><li>For Rabbit anti-<strong>NKX2.1<\/strong> (R1231) dilute 1:1000. For guinea pig anti-<strong>SCGB1A1<\/strong> (G210, Bl. 3\/24\/11) dilute 1:1500, for mouse anti-\u00a0<strong><a href=\"http:\/\/www.sigmaaldrich.com\/catalog\/product\/sigma\/t7451?lang=en\u00aeion=US\" target=\"_Blank\">TUBA1A<\/a><\/strong> (T7451, Sigma)\u00a0dilute 1:1000 in blocking buffer. Spin down in \u00b5fuge for 10 min, apply to tissue and incubate on tissue overnight @ 4<sup>o<\/sup>C.<\/li><\/ol><p><strong>Day 2 <\/strong><\/p><ol><li>Rinse slides in PBS-T 3X, 5 min.<\/li><li>Apply secondary antibody, Goat Alexa Fluor 488 anti-rabbit IgG (<a href=\"https:\/\/www.lifetechnologies.com\/order\/catalog\/product\/A11034?ICID=search-a11034\" target=\"_Blank\"><strong>A11034<\/strong><\/a>, Lot# 1298480, for anti-<strong>1<\/strong>), Goat Alexa Fluor 555 anti-guinea pig IgG (<a href=\"http:\/\/www.lifetechnologies.com\/order\/catalog\/product\/A21435?ICID=search-a21435\" target=\"_Blank\"><strong>A21435<\/strong><\/a>, Lot# 1235796, anti-<strong>SCGB1A1<\/strong>) at 1:200 in blocking buffer Donkey Alexa Fluor 647 anti-mouse IgG (<a href=\"https:\/\/www.lifetechnologies.com\/order\/catalog\/product\/A31571?ICID=search-a31571\" target=\"_Blank\">A31571<\/a>, Lot# 1549801, anti-<strong><a href=\"http:\/\/www.sigmaaldrich.com\/etc\/medialib\/docs\/Sigma\/Datasheet\/8\/t7451dat.Par.0001.File.tmp\/t7451dat.pdf\" target=\"_Blank\">TUBA1A<\/a><\/strong> (T7451, Sigma)) at 1:200, in blocking buffer. Spin down in \u00b5fuge for 10 min, apply to tissue and incubated at room temperature for 1 hour.<\/li><li>Rinse in PBS-T 3X, 5 min.<\/li><li>Dilute DAPI 1:2000 and apply to slides for 15 min.<\/li><li>Wash in PBS-T 3X, 5 min.<\/li><li>Rinse slides in 0.1M PB or TB, 3X, 5 min.<\/li><li>Add 1 drop of Prolong Gold anti-fade mounting medium (P36930).<\/li><li>Coverslip with Gold Seal Coverslip (Cat# 3422 Electron Microscopy Sciences, 22 X 22 mm).<\/li><li>Allow Prolong Gold to cure overnight at room temp in light sealed box.<\/li><li>Store slides in a light sealed box @ 4<sup>o<\/sup>C.<\/li><\/ol><p><strong>Tissue Used:\u00a0 <\/strong><strong><br \/><\/strong><\/p><p><strong>LMM.14.27.6.63 E18.5<\/strong><\/p><p>Gender: Male<\/p><p>Crown-to-Rump Length: 21.0 mm<\/p><p>Weight=1.32g<\/p><p><strong>Appendix<\/strong><\/p><p><strong>\u00a0<\/strong><\/p><p><strong>Antigen Retrieval Solution<\/strong><\/p><p>18ml Solution A- 0.1M Citric Acid (Sigma, C1909) pH 2.5<\/p><p>82ml Reagent B- 0.1M Sodium Citrate (Sigma, S4641 ) pH 8.2<\/p><p>1L dH<sub>2<\/sub>O<\/p><p>pH 6.0<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-4a83e1d8 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"4a83e1d8\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-74f430f8\" data-id=\"74f430f8\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-5f733faa elementor-widget elementor-widget-facebook-comments\" data-id=\"5f733faa\" data-element_type=\"widget\" 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target=\"target=\"_blank\"\"><img decoding=\"async\" class=\"lazyload amazingcarousel-image-img\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27300%27%20height%3D%27300%27%20viewBox%3D%270%200%20300%20300%27%3E%3Crect%20width%3D%27300%27%20height%3D%27300%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2015\/03\/2014-012-018_C57Bl6_E16.5_54.4.54_NKX2.1_SCGB1A1_TUBA1A-03_300X300.jpg\" alt=\"E16.5 C57BL6 NKX2.1, SCGB1A1, and TUBA1A Confocal Imaging\" data-description=\"\" \/><\/a>\n\t<div class=\"amazingcarousel-text\">\n\t\t<div class=\"amazingcarousel-text-bg\"><\/div>\n\t\t<div class=\"amazingcarousel-title\">E16.5 C57BL6 NKX2.1, SCGB1A1, and TUBA1A Confocal Imaging<\/div>\n\t<\/div>\n<\/div><\/div><\/li><li class=\"amazingcarousel-item\"><div class=\"amazingcarousel-item-container\"><div class=\"amazingcarousel-image\">\n\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=2022\" target=\"target=\"_blank\"\"><img decoding=\"async\" class=\"lazyload amazingcarousel-image-img\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27250%27%20height%3D%27250%27%20viewBox%3D%270%200%20250%20250%27%3E%3Crect%20width%3D%27250%27%20height%3D%27250%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2015\/04\/2014-012-018_C57Bl6_E18.5_LMM.14.27.6.63_NKX2.1_SCGB1A1_TUBA1A-06_250X250.jpg\" alt=\"E18.5 C57BL6 NKX2.1, SCGB1A1, and TUBA1A Confocal Imaging\" data-description=\"\" \/><\/a>\n\t<div class=\"amazingcarousel-text\">\n\t\t<div class=\"amazingcarousel-text-bg\"><\/div>\n\t\t<div class=\"amazingcarousel-title\">E18.5 C57BL6 NKX2.1, SCGB1A1, and TUBA1A Confocal Imaging<\/div>\n\t<\/div>\n<\/div><\/div><\/li><li class=\"amazingcarousel-item\"><div class=\"amazingcarousel-item-container\"><div class=\"amazingcarousel-image\">\n\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=8941\" target=\"_blank\"><img decoding=\"async\" class=\"lazyload amazingcarousel-image-img\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27300%27%20height%3D%27300%27%20viewBox%3D%270%200%20300%20300%27%3E%3Crect%20width%3D%27300%27%20height%3D%27300%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/07\/2016-07-06_C57BL6_PND03_LMM.16.83A.30_NKX2.1_SCGB1A1_TUBA1A-09-1.jpg\" alt=\"Post Natal Day 03 C57BL6 NKX2.1, SCGB1A1, and TUBA1A Confocal Imaging\" data-description=\"\" \/><\/a>\n\t<div class=\"amazingcarousel-text\">\n\t\t<div class=\"amazingcarousel-text-bg\"><\/div>\n\t\t<div class=\"amazingcarousel-title\">Post Natal Day 03 C57BL6 NKX2.1, SCGB1A1, and TUBA1A Confocal Imaging<\/div>\n\t<\/div>\n<\/div><\/div><\/li><li class=\"amazingcarousel-item\"><div class=\"amazingcarousel-item-container\"><div class=\"amazingcarousel-image\">\n\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=2168\" target=\"_blank\"><img decoding=\"async\" class=\"lazyload amazingcarousel-image-img\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27200%27%20height%3D%27200%27%20viewBox%3D%270%200%20200%20200%27%3E%3Crect%20width%3D%27200%27%20height%3D%27200%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2015\/04\/2015-002-023_C57BL6_PND7_LMM.15.17A.1.14_NKX2.1_SCGB1A1_TUBA1A-05_200X200.jpg\" alt=\"Post Natal Day 7 C57BL6 NKX2.1, SCGB1A1, and TUBA1A Confocal Imaging\" data-description=\"\" \/><\/a>\n\t<div class=\"amazingcarousel-text\">\n\t\t<div class=\"amazingcarousel-text-bg\"><\/div>\n\t\t<div class=\"amazingcarousel-title\">Post Natal Day 7 C57BL6 NKX2.1, SCGB1A1, and TUBA1A Confocal Imaging<\/div>\n\t<\/div>\n<\/div><\/div><\/li><li class=\"amazingcarousel-item\"><div class=\"amazingcarousel-item-container\"><div class=\"amazingcarousel-image\">\n\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=8956\" target=\"_blank\"><img decoding=\"async\" class=\"lazyload amazingcarousel-image-img\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27300%27%20height%3D%27300%27%20viewBox%3D%270%200%20300%20300%27%3E%3Crect%20width%3D%27300%27%20height%3D%27300%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/07\/2016-07-07_C57BL6_PND10_LMM.16.92A.6L.25_NKX2.1_SCGB1A1_TUBA1A-09-1.jpg\" alt=\"Post Natal Day 10 C57BL6 NKX2.1, SCGB1A1, and TUBA1A Confocal Imaging\" data-description=\"\" \/><\/a>\n\t<div class=\"amazingcarousel-text\">\n\t\t<div class=\"amazingcarousel-text-bg\"><\/div>\n\t\t<div class=\"amazingcarousel-title\">Post Natal Day 10 C57BL6 NKX2.1, SCGB1A1, and TUBA1A Confocal Imaging<\/div>\n\t<\/div>\n<\/div><\/div><\/li><li class=\"amazingcarousel-item\"><div class=\"amazingcarousel-item-container\"><div class=\"amazingcarousel-image\">\n\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=6529\" target=\"target=\"_Blank\"\"><img decoding=\"async\" class=\"lazyload amazingcarousel-image-img\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27300%27%20height%3D%27300%27%20viewBox%3D%270%200%20300%20300%27%3E%3Crect%20width%3D%27300%27%20height%3D%27300%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/04\/2015-08-19_C57BL6_PND14_LMM.15.34A.5L.36_NKX2.1_SCGB1A1_ACTA2-20-1.jpg\" alt=\"Postnatal Day 14 C57BL6 NKX2.1, SCGB1A1, and TUBA1A Confocal Imaging\" data-description=\"\" \/><\/a>\n\t<div class=\"amazingcarousel-text\">\n\t\t<div class=\"amazingcarousel-text-bg\"><\/div>\n\t\t<div class=\"amazingcarousel-title\">Postnatal Day 14 C57BL6 NKX2.1, SCGB1A1, and TUBA1A Confocal Imaging<\/div>\n\t<\/div>\n<\/div><\/div><\/li><li class=\"amazingcarousel-item\"><div class=\"amazingcarousel-item-container\"><div class=\"amazingcarousel-image\">\n\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=2174\" target=\"target=\"_Blank\"\"><img decoding=\"async\" class=\"lazyload amazingcarousel-image-img\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27200%27%20height%3D%27200%27%20viewBox%3D%270%200%20200%20200%27%3E%3Crect%20width%3D%27200%27%20height%3D%27200%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2015\/04\/2015-002-023_C57BL6_PND28_LMM.15.16A.6.28_NKX2.1_SCGB1A1_TUBA1A-10_200X200.jpg\" alt=\"Post Natal Day 28 C57BL6 NKX2.1, SCGB1A1, and TUBA1A Confocal Imaging\" data-description=\"\" \/><\/a>\n\t<div class=\"amazingcarousel-text\">\n\t\t<div class=\"amazingcarousel-text-bg\"><\/div>\n\t\t<div class=\"amazingcarousel-title\">Post Natal Day 28 C57BL6 NKX2.1, SCGB1A1, and TUBA1A Confocal Imaging<\/div>\n\t<\/div>\n<\/div><\/div><\/li><\/ul><div class=\"amazingcarousel-prev\"><\/div><div class=\"amazingcarousel-next\"><\/div><\/div><div class=\"amazingcarousel-nav\"><\/div><\/div><\/div><script>function wondercarousel_66_appendcss(csscode) {var head=document.head || document.getElementsByTagName(\"head\")[0];var style=document.createElement(\"style\");head.appendChild(style);style.type=\"text\/css\";if (style.styleSheet){style.styleSheet.cssText=csscode;} else {style.appendChild(document.createTextNode(csscode));}};wondercarousel_66_appendcss(\"@import url(https:\/\/fonts.googleapis.com\/css?family=Open+Sans);  #wonderplugincarousel-66 .amazingcarousel-image {\t \tposition: relative; \tpadding: 0px; }  #wonderplugincarousel-66 .amazingcarousel-image img { \tdisplay: block; \twidth: 100%; \tmax-width: 100%; \tborder: 0; \tmargin: 0; \tpadding: 0; \t-moz-border-radius: 0px; \t-webkit-border-radius: 0px; \tborder-radius: 0px; \t-moz-box-shadow:  0 1px 4px rgba(0, 0, 0, 0.2); \t-webkit-box-shadow: 0 1px 4px rgba(0, 0, 0, 0.2); \tbox-shadow: 0 1px 4px rgba(0, 0, 0, 0.2); }  #wonderplugincarousel-66 .amazingcarousel-text { \tposition:absolute; \tleft: 0px; \tbottom: 0px; \ttext-align:center; \twidth: 100%; \tdisplay: none; }  #wonderplugincarousel-66 .amazingcarousel-image:hover div.amazingcarousel-text {\t \tdisplay: block; }  #wonderplugincarousel-66 .amazingcarousel-text:hover { \tdisplay: block; }  #wonderplugincarousel-66 .amazingcarousel-text-bg { \tposition:absolute; \ttop: 0px; \tleft: 0px; \twidth: 100%; \theight: 100%; \tbackground-color: #333333; \topacity: 0.5; \tfilter: alpha(opacity=50); }  #wonderplugincarousel-66 .amazingcarousel-title { \tposition:relative; \tfont:14px \\\"Open Sans\\\", sans-serif; \tcolor:#ffffff; \ttext-align:center; 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}.amazingcarousel-socialmedia-icon { display:table-cell; width:32px; height:32px; font-size:18px; border-radius:50%; color:#fff; vertical-align:middle; text-align:center; cursor:pointer; padding:0;}.amazingcarousel-socialmedia-rotate { transition: transform .4s ease-in; } .amazingcarousel-socialmedia-rotate:hover { transform: rotate(360deg); }\");<\/script><\/div>\n\t\t<\/div>\n\t\t<div id=\"recent-posts-13\" class=\"widget widget_recent_entries\">\n\t\t<div class=\"heading\"><h4 class=\"widget-title\">Recent Experiments<\/h4><\/div>\n\t\t<ul>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=32066\">4 Year-Old Human Lung KRT14, MUC5B, and TUBA4A Confocal Imaging<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31223\">Alcian Blue Staining from PBR 3653 Patient with Cystic Fibrosis<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31202\">H&#038;E Staining from PBR 3383 Patient with Cystic Fibrosis<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31199\">H&#038;E Staining from PBR 3653 Patient with Cystic Fibrosis<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31196\">H&#038;E Staining of 31 Year-Old Human Donor 10<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31193\">H&#038;E Staining of 27 Year-Old Human Donor 3<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31190\">Alcian Blue Staining of 27 Year-Old Human Donor 3<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31186\">Alcian Blue Staining of 31 Year-Old Human Donor 10<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31182\">Alcian Blue Staining from PBR 3383 Patient with Cystic Fibrosis<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31024\">Alcian Blue Staining of 29 Year-Old Human Donor D175-10C1.21<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31019\">Alcian Blue Staining of 29 Year-Old Human Donor D175-11C4.14<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31016\">Alcian Blue Staining of 29 Year-Old Human Donor D175-11C2.11<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31013\">Alcian Blue Staining of 24 Year-Old Human Donor D071-23C2.19<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31010\">Alcian Blue Staining of 24 Year-Old Human Donor D071-17C2.20<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31007\">Alcian Blue Staining of 24 Year-Old Human Donor D071-16C3.27<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t<\/ul>\n\n\t\t<\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Immunofluorescence-\u00a0NKX2.1, SCGB1A1, \u00a0TUBA1A Purpose: Stain slides of 7\u00b5m frozen sections of C57BL\/6J E18.5 NKX2.1, SCGB1A1, and TUBA1A with antigen retrieval.\u00a0 \u00a0 Day 1 For Frozen tissue, rinse 2X in PBS, then 5 min in 4% PFA\/PBS, then rinse 1X in PBS. Antigen retrieval, pH 6.0 (times will vary according to microwave). Briefly equilibrate slides in [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":26027,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[83,274],"tags":[46,22,28,45],"class_list":["post-2022","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-e18-5","category-c57bl6","tag-cilia","tag-nkx2-1","tag-scgb1a1","tag-tuba1a"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v25.6 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>E18.5 C57BL6 NKX2.1, SCGB1A1, and TUBA1A Confocal Imaging -<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/research.cchmc.org\/lungimage\/?p=2022\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"E18.5 C57BL6 NKX2.1, SCGB1A1, and TUBA1A Confocal Imaging -\" \/>\n<meta property=\"og:description\" content=\"Immunofluorescence-\u00a0NKX2.1, SCGB1A1, \u00a0TUBA1A Purpose: Stain slides of 7\u00b5m frozen sections of C57BL\/6J E18.5 NKX2.1, SCGB1A1, and TUBA1A with antigen retrieval.\u00a0 \u00a0 Day 1 For Frozen tissue, rinse 2X in PBS, then 5 min in 4% PFA\/PBS, then rinse 1X in PBS. Antigen retrieval, pH 6.0 (times will vary according to microwave). Briefly equilibrate slides in [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/research.cchmc.org\/lungimage\/?p=2022\" \/>\n<meta property=\"article:published_time\" content=\"2015-04-03T00:13:22+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2018-08-17T22:43:14+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2015\/04\/2014-012-018_C57Bl6_E18.5_LMM.14.27.6.63_NKX2.1_SCGB1A1_TUBA1A-06.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"300\" \/>\n\t<meta property=\"og:image:height\" content=\"300\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"kitc5i\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"kitc5i\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" 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