{"id":21480,"date":"2018-04-20T17:54:33","date_gmt":"2018-04-20T17:54:33","guid":{"rendered":"https:\/\/research.cchmc.org\/lungimage\/?p=21480"},"modified":"2018-10-17T18:29:05","modified_gmt":"2018-10-17T18:29:05","slug":"8-month-old-human-lung-immunofluorescence-and-confocal-imaging-bpd-donor-d086-showing-expressions-of-hopx-ager-and-acta2","status":"publish","type":"post","link":"https:\/\/research.cchmc.org\/lungimage\/?p=21480","title":{"rendered":"8 Month-Old Human Lung Immunofluorescence and Confocal Imaging BPD Donor D086 Showing Expressions of HOPX, AGER, and ACTA2"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"21480\" class=\"elementor elementor-21480 elementor-bc-flex-widget\" data-elementor-post-type=\"post\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-492188b7 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"492188b7\" 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Showing Expressions of HOPX, AGER, and ACTA2\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">8 Month-Old Human Lung  Confocal Imaging BPD Donor D086 Showing Expressions of HOPX, AGER, and ACTA2<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/04\/2017-10-05_LMH-17-D086-LLL-8A3.11_HOPX_AGER_ACTA2-02-1.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/04\/2017-10-05_LMH-17-D086-LLL-8A3.11_HOPX_AGER_ACTA2-02-1.jpg\" alt=\"8 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data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/04\/2017-10-05_LMH-17-D086-LLL-8A3.11_HOPX_AGER_ACTA2-03-1.jpg\" alt=\"8 Month-Old Human Lung  Confocal Imaging BPD Donor D086 Showing Expressions of HOPX, AGER, and ACTA2\" data-description=\"60X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">8 Month-Old Human Lung  Confocal Imaging BPD Donor D086 Showing Expressions of HOPX, AGER, and ACTA2<\/div><div class=\"html5gallery-description\">60X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/04\/2017-10-05_LMH-17-D086-LLL-8A3.11_HOPX_AGER_ACTA2-04-1.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/04\/2017-10-05_LMH-17-D086-LLL-8A3.11_HOPX_AGER_ACTA2-04-1.jpg\" alt=\"8 Month-Old Human Lung  Confocal Imaging BPD Donor D086 Showing Expressions of HOPX, AGER, and ACTA2\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">8 Month-Old Human Lung  Confocal Imaging BPD Donor D086 Showing Expressions of HOPX, AGER, and ACTA2<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/04\/2017-10-05_LMH-17-D086-LLL-8A3.11_HOPX_AGER_ACTA2-05-1.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/04\/2017-10-05_LMH-17-D086-LLL-8A3.11_HOPX_AGER_ACTA2-05-1.jpg\" alt=\"8 Month-Old Human Lung  Confocal Imaging BPD Donor D086 Showing Expressions of HOPX, AGER, and ACTA2\" data-description=\"60X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">8 Month-Old Human Lung  Confocal Imaging BPD Donor D086 Showing Expressions of HOPX, AGER, and ACTA2<\/div><div class=\"html5gallery-description\">60X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/04\/2017-10-05_LMH-17-D086-LLL-8A3.11_HOPX_AGER_ACTA2-06-1.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/04\/2017-10-05_LMH-17-D086-LLL-8A3.11_HOPX_AGER_ACTA2-06-1.jpg\" alt=\"8 Month-Old Human Lung  Confocal Imaging BPD Donor D086 Showing Expressions of HOPX, AGER, and ACTA2\" data-description=\"60X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">8 Month-Old Human Lung  Confocal Imaging BPD Donor D086 Showing Expressions of HOPX, AGER, and ACTA2<\/div><div class=\"html5gallery-description\">60X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/04\/2017-10-05_LMH-17-D086-LLL-8A3.11_HOPX_AGER_ACTA2-07-1.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/04\/2017-10-05_LMH-17-D086-LLL-8A3.11_HOPX_AGER_ACTA2-07-1.jpg\" alt=\"8 Month-Old Human Lung  Confocal Imaging BPD Donor D086 Showing Expressions of HOPX, AGER, and ACTA2\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">8 Month-Old Human Lung  Confocal Imaging BPD Donor D086 Showing Expressions of HOPX, AGER, and ACTA2<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/04\/2017-10-05_LMH-17-D086-LLL-8A3.11_HOPX_AGER_ACTA2-08-1.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/04\/2017-10-05_LMH-17-D086-LLL-8A3.11_HOPX_AGER_ACTA2-08-1.jpg\" alt=\"8 Month-Old Human Lung  Confocal Imaging BPD Donor D086 Showing Expressions of HOPX, AGER, and ACTA2\" data-description=\"60X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">8 Month-Old Human Lung  Confocal Imaging BPD Donor D086 Showing Expressions of HOPX, AGER, and ACTA2<\/div><div class=\"html5gallery-description\">60X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/04\/2017-10-05_LMH-17-D086-LLL-8A3.11_HOPX_AGER_ACTA2-09-2.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/04\/2017-10-05_LMH-17-D086-LLL-8A3.11_HOPX_AGER_ACTA2-09-2.jpg\" alt=\"8 Month-Old Human Lung  Confocal Imaging BPD Donor D086 Showing Expressions of HOPX, AGER, and ACTA2\" data-description=\"60X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">8 Month-Old Human Lung  Confocal Imaging BPD Donor D086 Showing Expressions of HOPX, AGER, and ACTA2<\/div><div class=\"html5gallery-description\">60X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/04\/2017-10-05_LMH-17-D086-LLL-8A3.11_HOPX_AGER_ACTA2-10-1.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/04\/2017-10-05_LMH-17-D086-LLL-8A3.11_HOPX_AGER_ACTA2-10-1.jpg\" alt=\"8 Month-Old Human Lung  Confocal Imaging BPD Donor D086 Showing Expressions of HOPX, AGER, and ACTA2\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">8 Month-Old Human Lung  Confocal Imaging BPD Donor D086 Showing Expressions of HOPX, AGER, and ACTA2<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/04\/2017-10-05_LMH-17-D086-LLL-8A3.11_HOPX_AGER_ACTA2-11-1.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/04\/2017-10-05_LMH-17-D086-LLL-8A3.11_HOPX_AGER_ACTA2-11-1.jpg\" alt=\"8 Month-Old Human Lung  Confocal Imaging BPD Donor D086 Showing Expressions of HOPX, AGER, and ACTA2\" data-description=\"60X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">8 Month-Old Human Lung  Confocal Imaging BPD Donor D086 Showing Expressions of HOPX, AGER, and ACTA2<\/div><div class=\"html5gallery-description\">60X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/04\/2017-10-05_LMH-17-D086-LLL-8A3.11_HOPX_AGER_ACTA2-12-1.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/04\/2017-10-05_LMH-17-D086-LLL-8A3.11_HOPX_AGER_ACTA2-12-1.jpg\" alt=\"8 Month-Old Human Lung  Confocal Imaging BPD Donor D086 Showing Expressions of HOPX, AGER, and ACTA2\" data-description=\"60X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">8 Month-Old Human Lung  Confocal Imaging BPD Donor D086 Showing Expressions of HOPX, AGER, and ACTA2<\/div><div class=\"html5gallery-description\">60X<\/div><\/div><\/div><\/div><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-4fa65ea7 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"4fa65ea7\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-33c217b8\" data-id=\"33c217b8\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-6f4d925d elementor-widget elementor-widget-template\" data-id=\"6f4d925d\" data-element_type=\"widget\" data-widget_type=\"template.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-template\">\n\t\t\t\t\t<div data-elementor-type=\"section\" data-elementor-id=\"26498\" class=\"elementor elementor-26498 elementor-bc-flex-widget\" data-elementor-post-type=\"elementor_library\">\n\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-4650a05 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"4650a05\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-951322a\" data-id=\"951322a\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-4f951ba\" data-id=\"4f951ba\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-0022067\" data-id=\"0022067\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-737d492c elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"737d492c\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-21f0c407\" data-id=\"21f0c407\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-7731faf8 elementor-widget elementor-widget-text-editor\" data-id=\"7731faf8\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div style=\"font-family: 'PT Sans', Arial, Helvetica, sans-serif; background-color: rgba(255, 255, 255, 0); background-position: left top; background-repeat: no-repeat; padding: 20px 0px; border-top: 0px solid #eae9e9; border-bottom: 0px solid #eae9e9; border-right-color: #eae9e9; border-left-color: #eae9e9;\"><div style=\"width: 1150px;\"><div style=\"margin-top: 0px; margin-bottom: 0px;\"><div style=\"padding: 0px; background-position: left top; background-repeat: no-repeat; background-size: cover;\" data-bg-url=\"\"><div><p style=\"margin: 0px 0px 20px;\">Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from\u00a0the University of Rochester Medical Center<\/p><p style=\"margin: 0px 0px 20px;\"><a href=\"https:\/\/www.urmc.rochester.edu\/labs\/Pryhuber-Lab\/\" target=\"_blank\" rel=\"noopener\"><img class=\"lazyload\" decoding=\"async\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27200%27%20height%3D%2748%27%20viewBox%3D%270%200%20200%2048%27%3E%3Crect%20width%3D%27200%27%20height%3D%2748%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2015\/07\/Pryhuber_Lab.jpg\" alt=\"Pryhuber_Lab\" width=\"200\" height=\"48\" \/><\/a><\/p><p style=\"margin: 0px 0px 20px;\"><span style=\"font-size: 14pt;\"><span style=\"text-align: center; background-color: rgba(255, 255, 255, 0); letter-spacing: 0px; font-weight: bold;\">\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 Immunofluorescence-\u00a0<\/span><span style=\"text-align: center; background-color: rgba(255, 255, 255, 0); letter-spacing: 0px; font-weight: bold;\">\u00a0<\/span><a style=\"text-align: center; background-color: rgba(255, 255, 255, 0); letter-spacing: 0px;\" href=\"http:\/\/www.scbt.com\/datasheet-30216-hop-fl-73-antibody.html\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: bold;\">HOPX<\/span><\/a><span style=\"text-align: center; background-color: rgba(255, 255, 255, 0); letter-spacing: 0px; font-weight: bold;\">,<\/span><span style=\"text-align: center; background-color: rgba(255, 255, 255, 0); letter-spacing: 0px;\">\u00a0<\/span><a style=\"text-align: center; background-color: rgba(255, 255, 255, 0); letter-spacing: 0px;\" href=\"https:\/\/www.rndsystems.com\/products\/human-rage-antibody_af1145\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: bold;\">AGER<\/span><\/a><span style=\"text-align: center; background-color: rgba(255, 255, 255, 0); letter-spacing: 0px; font-weight: bold;\">,\u00a0<\/span><span style=\"text-align: center; background-color: rgba(255, 255, 255, 0); letter-spacing: 0px;\">and<\/span><span style=\"text-align: center; background-color: rgba(255, 255, 255, 0); letter-spacing: 0px;\">\u00a0<\/span><a style=\"text-align: center; background-color: rgba(255, 255, 255, 0); letter-spacing: 0px;\" href=\"http:\/\/www.sigmaaldrich.com\/catalog\/product\/sigma\/a5228?lang=en&amp;region=US\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: bold;\">ACTA2<\/span><\/a><\/span><\/p><p style=\"margin: 0px 0px 20px;\"><span style=\"font-weight: bold;\">\u00a0<\/span><\/p><p style=\"margin: 0px 0px 20px;\"><span style=\"font-weight: bold;\">Purpose:\u00a0\u00a0<\/span>Stain slides of 7\u00b5m frozen sections of BPD human lung from HTC for\u00a0<a href=\"http:\/\/www.scbt.com\/datasheet-30216-hop-fl-73-antibody.html\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: bold;\">HOPX<\/span><\/a><span style=\"font-weight: bold;\">,\u00a0<\/span><a href=\"https:\/\/www.rndsystems.com\/products\/human-rage-antibody_af1145\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: bold;\">AGER<\/span><\/a><span style=\"font-weight: bold;\">, and\u00a0<\/span><a href=\"http:\/\/www.sigmaaldrich.com\/catalog\/product\/sigma\/a5228?lang=en&amp;region=US\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: bold;\">ACTA2<\/span><\/a>\u00a0with antigen retrieval.<\/p><p style=\"margin: 0px 0px 20px;\"><span style=\"font-weight: bold;\">Day 1<\/span><\/p><ol><li>For Frozen tissue, rinse 2X in PBS, then 5 min in 4% PFA\/PBS, then rinse 1X in PBS.<\/li><li>Briefly equilibrate slides in Antigen Retrieval Buffer.<\/li><li>Antigen retrieval, pH 6.0 (times will vary according to microwave).<\/li><li>10 mM sodium citrate, pH 6.0, and heat in a microwave at 96<sup>o<\/sup>C.\u00a0*We usually do a series of three runs (6-7 minutes each run) <br \/>to equal the time\/temp because of evaporation (refill coplin jars with dH<sub>2<\/sub>O).<\/li><li>Microwave according to instructions on microwave.<\/li><li>Cool on countertop, 15 min.<\/li><li>Rinse with dH<sub>2<\/sub>O<\/li><li>1X PBS, 5 min.<\/li><li>Block in 4% Donkey serum\/PBS-T, 2 hours at RT.<\/li><li>For rabbit anti-\u00a0<a href=\"http:\/\/www.scbt.com\/datasheet-30216-hop-fl-73-antibody.html\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: bold;\">HOPX<\/span><\/a>\u00a0(<a href=\"http:\/\/www.scbt.com\/datasheet-30216-hop-fl-73-antibody.html\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: bold;\">SC-30216<\/span><\/a>, Lot # C2212, Santa Cruz) dilute 1:200, For goat anti-<a href=\"https:\/\/www.rndsystems.com\/products\/human-rage-antibody_af1145\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: bold;\">AGER<\/span><\/a>\u00a0(<a href=\"https:\/\/www.rndsystems.com\/products\/human-rage-antibody_af1145\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: bold;\">AF1145<\/span><\/a>, R&amp;D Systems) dilute <br \/>1:100, for mouse anti-<a href=\"http:\/\/www.sigmaaldrich.com\/catalog\/product\/sigma\/a5228?lang=en&amp;region=US\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: bold;\">ACTA2<\/span><\/a>\u00a0(<a href=\"http:\/\/www.sigmaaldrich.com\/catalog\/product\/sigma\/a5228?lang=en%C2%AEion=US\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: bold;\">\u03b1 Smooth muscle actin<\/span><\/a>, A5228, Sigma)\u00a0dilute 1:2000 in blocking buffer. Spin down in \u00b5fuge for <br \/>10 minutes and incubate on tissue overnight @ 4<sup>o<\/sup>C.<\/li><\/ol><p style=\"margin: 0px 0px 20px;\"><span style=\"font-weight: bold;\">Day 2<\/span><\/p><ol><li>Rinse slides in PBS-T 3X, 5 min.<\/li><li>Apply secondary antibody Apply secondary antibody , Donkey Alexa Fluor 488 anti-rabbit IgG (<a href=\"https:\/\/www.lifetechnologies.com\/order\/catalog\/product\/A21206?ICID=search-a21206\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: bold;\">A21206<\/span><\/a>, Lot# 1608521, for <br \/>anti-<a href=\"http:\/\/www.scbt.com\/datasheet-30216-hop-fl-73-antibody.html\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: bold;\">HOPX<\/span><\/a>), ), Donkey Alexa Fluor 568 anti-goat IgG (<a href=\"http:\/\/www.lifetechnologies.com\/order\/catalog\/product\/A11057?ICID=search-a11057\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: bold;\">A11057<\/span><\/a>, Lot# 1485187, for anti-<a href=\"https:\/\/www.rndsystems.com\/products\/human-rage-antibody_af1145\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: bold;\">AGER<\/span><\/a>), Donkey Alexa Fluor 647 <br \/>anti-mouse IgG (<a href=\"https:\/\/www.lifetechnologies.com\/order\/catalog\/product\/A31571?ICID=search-a31571\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: bold;\">A31571<\/span><\/a>, Lot# 1549801, anti-<a href=\"http:\/\/www.sigmaaldrich.com\/catalog\/product\/sigma\/a5228?lang=en&amp;region=US\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: bold;\">ACTA2<\/span><\/a>) at 1:200, in blocking buffer. Spin down in \u00b5fuge for 10 min, apply to <br \/>tissue and incubated at room temperature for 1 hour.<\/li><li>Rinse in PBS-T 3X, 5 min.<\/li><li>Dilute DAPI 1:2000 and apply to slides for 10 min.<\/li><li>Wash in PBS-T 3X, 5 min.<\/li><li>Rinse slides in 0.1M PB or TB, 3X, 5 min.<\/li><li>Add 1 drop of Prolong Gold anti-fade mounting medium (P36930).<\/li><li>Coverslip with Gold Seal Coverslip (Cat# 3422 Electron Microscopy Sciences, 22 X 22 mm).<\/li><li>Allow Prolong Gold to cure overnight at room temp in light sealed box.<\/li><\/ol><ol start=\"10\"><li>Store slides in light sealed box @ 4<sup>o<\/sup>C<\/li><\/ol><p style=\"margin: 0px 0px 20px;\"><span style=\"font-weight: bold;\">\u00a0<\/span><\/p><p style=\"margin: 0px 0px 20px;\"><span style=\"font-weight: bold;\">\u00a0<\/span><\/p><p style=\"margin: 0px 0px 20px;\"><span style=\"font-weight: bold;\">Tissue Used:<br \/><\/span><span style=\"font-weight: bold; background-color: rgba(255, 255, 255, 0); letter-spacing: 0px;\">LMH-17-D086-LLL-8A3.11<br \/><\/span><span style=\"font-weight: bold; background-color: rgba(255, 255, 255, 0); letter-spacing: 0px;\">Age: 8 Months-old<br \/><\/span><span style=\"font-weight: bold; background-color: rgba(255, 255, 255, 0); letter-spacing: 0px;\">Gender: Male<\/span><\/p><p style=\"margin: 0px 0px 20px;\"><span style=\"font-weight: bold;\">\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/span><\/p><p style=\"margin: 0px 0px 20px;\"><span style=\"font-weight: bold; background-color: rgba(255, 255, 255, 0); letter-spacing: 0px;\">Appendix<br \/><\/span><span style=\"background-color: rgba(255, 255, 255, 0); font-weight: bold; letter-spacing: 0px;\">Antigen Retrieval Solution<\/span><\/p><p style=\"margin: 0px 0px 20px;\">18ml Solution A- 0.1M Citric Acid (Sigma, C1909) pH 2.5<\/p><p style=\"margin: 0px 0px 20px;\">82ml Reagent B- 0.1M Sodium Citrate (Sigma, S4641 ) pH 8.2<\/p><p style=\"margin: 0px 0px 20px;\">1L dH<sub>2<\/sub>O<\/p><p style=\"margin: 0px 0px 20px;\">pH 6.0<\/p><\/div><div>\u00a0<\/div><\/div><\/div><\/div><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-24d6bc6d elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"24d6bc6d\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-19cc1ff5\" data-id=\"19cc1ff5\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-4412279f elementor-widget elementor-widget-facebook-comments\" 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href=\"https:\/\/research.cchmc.org\/lungimage\/?p=8280\" target=\"target=\"_Blank\"\"><img decoding=\"async\" class=\"lazyload amazingcarousel-image-img\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27300%27%20height%3D%27300%27%20viewBox%3D%270%200%20300%20300%27%3E%3Crect%20width%3D%27300%27%20height%3D%27300%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/05\/2016-05-17_LMH-15-D024-LLL-8A2.15_HOPX_AGER_ACTA2-07-1.jpg\" alt=\"4 Month Human Lung HOPX, AGER, and ACTA2 Confocal Imaging\" data-description=\"\" \/><\/a>\n\t<div class=\"amazingcarousel-text\">\n\t\t<div class=\"amazingcarousel-text-bg\"><\/div>\n\t\t<div class=\"amazingcarousel-title\">4 Month Human Lung HOPX, AGER, and ACTA2 Confocal Imaging<\/div>\n\t<\/div>\n<\/div><\/div><\/li><li class=\"amazingcarousel-item\"><div class=\"amazingcarousel-item-container\"><div class=\"amazingcarousel-image\">\n\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=8331\" target=\"target=\"Blank\"\"><img decoding=\"async\" class=\"lazyload amazingcarousel-image-img\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27300%27%20height%3D%27300%27%20viewBox%3D%270%200%20300%20300%27%3E%3Crect%20width%3D%27300%27%20height%3D%27300%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/05\/2016-05-18_LMH-15-D003-LLL-4B3.18_HOPX_AGER_ACTA2-08-1.jpg\" alt=\"9 Month Old Human Lung HOPX, AGER, and ACTA2 Confocal Imaging\" data-description=\"\" \/><\/a>\n\t<div class=\"amazingcarousel-text\">\n\t\t<div class=\"amazingcarousel-text-bg\"><\/div>\n\t\t<div class=\"amazingcarousel-title\">9 Month Old Human Lung HOPX, AGER, and ACTA2 Confocal Imaging<\/div>\n\t<\/div>\n<\/div><\/div><\/li><li class=\"amazingcarousel-item\"><div class=\"amazingcarousel-item-container\"><div class=\"amazingcarousel-image\">\n\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=8347\" target=\"target=\"Blank\"\"><img decoding=\"async\" class=\"lazyload amazingcarousel-image-img\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27300%27%20height%3D%27300%27%20viewBox%3D%270%200%20300%20300%27%3E%3Crect%20width%3D%27300%27%20height%3D%27300%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/05\/2016-05-18_LMH-15-D007-LLL-5C4.4_HOPX_AGER_ACTA2-09-1.jpg\" alt=\"4 Year Old Human Lung HOPX, AGER, and ACTA2 Confocal Imaging\" data-description=\"\" \/><\/a>\n\t<div class=\"amazingcarousel-text\">\n\t\t<div class=\"amazingcarousel-text-bg\"><\/div>\n\t\t<div class=\"amazingcarousel-title\">4 Year Old Human Lung HOPX, AGER, and ACTA2 Confocal Imaging<\/div>\n\t<\/div>\n<\/div><\/div><\/li><li class=\"amazingcarousel-item\"><div class=\"amazingcarousel-item-container\"><div class=\"amazingcarousel-image\">\n\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=21480\" target=\"_blank\"><img decoding=\"async\" class=\"lazyload amazingcarousel-image-img\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27300%27%20height%3D%27300%27%20viewBox%3D%270%200%20300%20300%27%3E%3Crect%20width%3D%27300%27%20height%3D%27300%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/04\/2017-10-05_LMH-17-D086-LLL-8A3.11_HOPX_AGER_ACTA2-09-1.jpg\" alt=\"8 Month-Old Human Lung Immunofluorescence and Confocal Imaging BPD Donor D086 Showing Expressions of HOPX, AGER, and ACTA2\" data-description=\"\" \/><\/a>\n\t<div class=\"amazingcarousel-text\">\n\t\t<div class=\"amazingcarousel-text-bg\"><\/div>\n\t\t<div class=\"amazingcarousel-title\">8 Month-Old Human Lung Immunofluorescence and Confocal Imaging BPD Donor D086 Showing Expressions of HOPX, AGER, and ACTA2<\/div>\n\t<\/div>\n<\/div><\/div><\/li><\/ul><div class=\"amazingcarousel-prev\"><\/div><div class=\"amazingcarousel-next\"><\/div><\/div><div class=\"amazingcarousel-nav\"><\/div><\/div><\/div><script>function wondercarousel_157_appendcss(csscode) {var head=document.head || document.getElementsByTagName(\"head\")[0];var style=document.createElement(\"style\");head.appendChild(style);style.type=\"text\/css\";if (style.styleSheet){style.styleSheet.cssText=csscode;} else {style.appendChild(document.createTextNode(csscode));}};wondercarousel_157_appendcss(\"@import url(https:\/\/fonts.googleapis.com\/css?family=Open+Sans);  #wonderplugincarousel-157 .amazingcarousel-image {\t \tposition: relative; \tpadding: 0px; }  #wonderplugincarousel-157 .amazingcarousel-image img { \tdisplay: block; \twidth: 100%; \tmax-width: 100%; \tborder: 0; \tmargin: 0; \tpadding: 0; \t-moz-border-radius: 0px; \t-webkit-border-radius: 0px; \tborder-radius: 0px; \t-moz-box-shadow:  0 1px 4px rgba(0, 0, 0, 0.2); \t-webkit-box-shadow: 0 1px 4px rgba(0, 0, 0, 0.2); \tbox-shadow: 0 1px 4px rgba(0, 0, 0, 0.2); }  #wonderplugincarousel-157 .amazingcarousel-text { \tposition:absolute; \tleft: 0px; \tbottom: 0px; \ttext-align:center; \twidth: 100%; \tdisplay: none; }  #wonderplugincarousel-157 .amazingcarousel-image:hover div.amazingcarousel-text {\t \tdisplay: block; }  #wonderplugincarousel-157 .amazingcarousel-text:hover { \tdisplay: block; }  #wonderplugincarousel-157 .amazingcarousel-text-bg { \tposition:absolute; \ttop: 0px; \tleft: 0px; \twidth: 100%; \theight: 100%; \tbackground-color: #333333; \topacity: 0.5; \tfilter: alpha(opacity=50); }  #wonderplugincarousel-157 .amazingcarousel-title { \tposition:relative; \tfont:14px \\\"Open Sans\\\", sans-serif; \tcolor:#ffffff; \ttext-align:center; \twidth: 100%; \tpadding: 10px 0px; }  #wonderplugincarousel-157 .amazingcarousel-list-container {  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border-radius:50%; color:#fff; vertical-align:middle; text-align:center; cursor:pointer; padding:0;}.amazingcarousel-socialmedia-rotate { transition: transform .4s ease-in; } .amazingcarousel-socialmedia-rotate:hover { transform: rotate(360deg); }\");<\/script><\/div>\n\t\t<\/div>\n\t\t<div id=\"recent-posts-48\" class=\"widget widget_recent_entries\">\n\t\t<div class=\"heading\"><h4 class=\"widget-title\">Recent Experiments<\/h4><\/div>\n\t\t<ul>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=32066\">4 Year-Old Human Lung KRT14, MUC5B, and TUBA4A Confocal Imaging<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31223\">Alcian Blue Staining from PBR 3653 Patient with Cystic Fibrosis<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31202\">H&#038;E Staining from PBR 3383 Patient with Cystic Fibrosis<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31199\">H&#038;E Staining from PBR 3653 Patient with Cystic Fibrosis<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31196\">H&#038;E Staining of 31 Year-Old Human Donor 10<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31193\">H&#038;E Staining of 27 Year-Old Human Donor 3<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31190\">Alcian Blue Staining of 27 Year-Old Human Donor 3<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31186\">Alcian Blue Staining of 31 Year-Old Human Donor 10<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31182\">Alcian Blue Staining from PBR 3383 Patient with Cystic Fibrosis<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31024\">Alcian Blue Staining of 29 Year-Old Human Donor D175-10C1.21<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31019\">Alcian Blue Staining of 29 Year-Old Human Donor D175-11C4.14<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31016\">Alcian Blue Staining of 29 Year-Old Human Donor D175-11C2.11<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31013\">Alcian Blue Staining of 24 Year-Old Human Donor D071-23C2.19<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31010\">Alcian Blue Staining of 24 Year-Old Human Donor D071-17C2.20<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31007\">Alcian Blue Staining of 24 Year-Old Human Donor D071-16C3.27<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t<\/ul>\n\n\t\t<\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from\u00a0the University of Rochester Medical Center \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 Immunofluorescence-\u00a0\u00a0HOPX,\u00a0AGER,\u00a0and\u00a0ACTA2 \u00a0 Purpose:\u00a0\u00a0Stain slides of 7\u00b5m frozen sections of BPD human lung from HTC for\u00a0HOPX,\u00a0AGER, and\u00a0ACTA2\u00a0with antigen retrieval. [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":21481,"comment_status":"closed","ping_status":"open","sticky":false,"template":"elementor_header_footer","format":"standard","meta":{"footnotes":""},"categories":[290,286,103],"tags":[5,108,19],"class_list":["post-21480","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-bpd-confocal-imaging","category-human-lung-disease-confocal-imaging","category-postnatal","tag-acta2","tag-ager","tag-hopx"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v25.6 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>8 Month-Old Human Lung Immunofluorescence and Confocal Imaging BPD Donor D086 Showing Expressions of HOPX, AGER, and ACTA2 -<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/research.cchmc.org\/lungimage\/?p=21480\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"8 Month-Old Human Lung Immunofluorescence and Confocal Imaging BPD Donor D086 Showing Expressions of HOPX, AGER, and ACTA2 -\" \/>\n<meta property=\"og:description\" content=\"Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from\u00a0the University of Rochester Medical Center \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 Immunofluorescence-\u00a0\u00a0HOPX,\u00a0AGER,\u00a0and\u00a0ACTA2 \u00a0 Purpose:\u00a0\u00a0Stain slides of 7\u00b5m frozen sections of BPD human lung from HTC for\u00a0HOPX,\u00a0AGER, and\u00a0ACTA2\u00a0with antigen retrieval. [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/research.cchmc.org\/lungimage\/?p=21480\" \/>\n<meta property=\"article:published_time\" content=\"2018-04-20T17:54:33+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2018-10-17T18:29:05+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/04\/2017-10-05_LMH-17-D086-LLL-8A3.11_HOPX_AGER_ACTA2-09-1.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"300\" \/>\n\t<meta property=\"og:image:height\" content=\"300\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"kitc5i\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"kitc5i\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"2 minutes\" \/>\n<script 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