{"id":24825,"date":"2018-07-10T02:21:43","date_gmt":"2018-07-10T02:21:43","guid":{"rendered":"https:\/\/research.cchmc.org\/lungimage\/?p=24825"},"modified":"2018-09-09T22:52:26","modified_gmt":"2018-09-09T22:52:26","slug":"5-month-old-human-lung-enhanced-resolution-confocal-imaging-from-donor-39503-c2-containing-an-abca3-homozygous-missense-mutation-showing-expression-of-sftpb-and-abca3","status":"publish","type":"post","link":"https:\/\/research.cchmc.org\/lungimage\/?p=24825","title":{"rendered":"21 Month-Old Human Lung Enhanced Resolution Confocal Imaging From Donor 17235-B2 containing an ABCA3 Homozygous Missense Mutation Showing Expression of SFTPB and ABCA3"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"24825\" class=\"elementor elementor-24825 elementor-bc-flex-widget\" data-elementor-post-type=\"post\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-65b898f6 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"65b898f6\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-7a76de05\" data-id=\"7a76de05\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-b68b85c elementor-widget elementor-widget-theme-post-title elementor-page-title elementor-widget-heading\" data-id=\"b68b85c\" data-element_type=\"widget\" data-widget_type=\"theme-post-title.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h1 class=\"elementor-heading-title elementor-size-large\">21 Month-Old Human Lung Enhanced Resolution Confocal Imaging From Donor 17235-B2 containing an ABCA3 Homozygous Missense Mutation Showing Expression of SFTPB and ABCA3<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-1850c5f9 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"1850c5f9\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-3338e8f8\" data-id=\"3338e8f8\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-63b429c8 elementor-widget elementor-widget-shortcode\" data-id=\"63b429c8\" data-element_type=\"widget\" data-widget_type=\"shortcode.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-shortcode\"><div 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Homozygous Missence ABCA3 Mutation\" data-description=\"Enhanced resolution confocal imaging human lung containing ABCA3 homozygous missense mutation for SFTPB and ABCA3 Proteins\" \/><\/li><li><img decoding=\"async\" class=\"lazyload amazingslider-thumbnail-elem amazingsliderthumbnailimg\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" data-orig-src=\"https:\/\/img.youtube.com\/vi\/JwYGKVm0ZFg\/0.jpg\" alt=\"21 Month-Old Human Lung 17235 Containing Homozygous Missence ABCA3 Mutation\" title=\"21 Month-Old Human Lung 17235 Containing Homozygous Missence ABCA3 Mutation\" data-description=\"Enhanced resolution confocal imaging human lung containing ABCA3 homozygous missense mutation for SFTPB and ABCA3 Proteins\" \/><\/li><li><img decoding=\"async\" class=\"lazyload amazingslider-thumbnail-elem amazingsliderthumbnailimg\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" data-orig-src=\"https:\/\/img.youtube.com\/vi\/87ey3_9Kchs\/0.jpg\" alt=\"21 Month-Old Human Lung 17235 Containing Homozygous Missence ABCA3 Mutation\" title=\"21 Month-Old Human Lung 17235 Containing Homozygous Missence ABCA3 Mutation\" data-description=\"Enhanced resolution confocal imaging human lung containing ABCA3 homozygous missense mutation for SFTPB and ABCA3 Proteins\" \/><\/li><li><img decoding=\"async\" class=\"lazyload amazingslider-thumbnail-elem amazingsliderthumbnailimg\" src=\"data:image\/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw==\" data-orig-src=\"https:\/\/img.youtube.com\/vi\/eMFYoHX50yk\/0.jpg\" alt=\"21 Month-Old Human Lung 17235 Containing Homozygous Missence ABCA3 Mutation\" title=\"21 Month-Old Human Lung 17235 Containing Homozygous Missence ABCA3 Mutation\" data-description=\"Enhanced resolution confocal imaging human lung containing ABCA3 homozygous missense mutation for SFTPB and ABCA3 Proteins\" \/><\/li><\/ul><\/div><\/div><div 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padding:12px; text-align:left;&quot;,&quot;textbgcss&quot;:&quot;display:none;&quot;,&quot;titlecss&quot;:&quot;display:block; position:relative; font:bold 14px Georgia,serif,Arial; color:#fff;&quot;,&quot;descriptioncss&quot;:&quot;display:block; position:relative; font:12px Georgia,serif,Arial; color:#fff; margin-top:8px;&quot;,&quot;buttoncss&quot;:&quot;display:block; position:relative; margin-top:8px;&quot;,&quot;texteffectresponsive&quot;:true,&quot;texteffectresponsivesize&quot;:640,&quot;titlecssresponsive&quot;:&quot;font-size:12px;&quot;,&quot;descriptioncssresponsive&quot;:&quot;display:none !important;&quot;,&quot;buttoncssresponsive&quot;:&quot;&quot;,&quot;addgooglefonts&quot;:false,&quot;googlefonts&quot;:&quot;&quot;,&quot;textleftrightpercentforstatic&quot;:40}}\"><\/div><\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-7a07ce79 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"7a07ce79\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-25d22b2\" data-id=\"25d22b2\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-7247cee6 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"7247cee6\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-6c247f6f\" data-id=\"6c247f6f\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-54b7d454 elementor-widget elementor-widget-text-editor\" data-id=\"54b7d454\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"font-family: 'PT Sans', sans-serif; text-align: center;\">\u00a0<\/p><p style=\"font-family: 'PT Sans', sans-serif; text-align: center;\" align=\"center\"><span style=\"font-size: 12pt;\"><span style=\"font-weight: bold;\"><span style=\"font-family: Calibri, sans-serif;\">Immunofluorescence- SFTPC, SFTPB , and\u00a0<\/span><\/span><a href=\"http:\/\/sevenhillsbioreagents.com\/collections\/antibodies\/products\/anti-abca3-mouse-17-h5-24\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: bold;\"><span style=\"font-family: Calibri, sans-serif;\">ABCA3<\/span><\/span><\/a><\/span><\/p><p style=\"font-family: 'PT Sans', sans-serif;\"><span style=\"font-size: 12pt;\"><span style=\"font-weight: bold;\"><span style=\"font-family: Calibri, sans-serif;\">\u00a0<\/span><\/span><\/span><\/p><p style=\"font-family: 'PT Sans', sans-serif;\"><span style=\"font-size: 12pt;\"><span style=\"font-weight: bold;\"><span style=\"font-family: Calibri, sans-serif;\">Purpose:\u00a0\u00a0<\/span><\/span><span style=\"font-family: Calibri, sans-serif;\">Stain slides of 5\u00b5m paraffin sections of 21 months-old postnatal human lung from HTC\u00a0<span style=\"font-weight: bold;\">SFTPC, SFTPB, and<\/span><\/span><a href=\"http:\/\/sevenhillsbioreagents.com\/collections\/antibodies\/products\/anti-abca3-mouse-17-h5-24\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: bold;\"><span style=\"font-family: Calibri, sans-serif;\">ABCA3<\/span><\/span><\/a><span style=\"font-weight: bold;\">\u00a0<\/span><span style=\"font-family: Calibri, sans-serif;\">with antigen retrieval.<\/span><\/span><\/p><p style=\"font-family: 'PT Sans', sans-serif;\"><span style=\"font-size: 12pt;\"><span style=\"font-weight: bold;\"><span style=\"font-family: Calibri, sans-serif;\">Day 1<\/span><\/span><\/span><\/p><ol style=\"font-family: 'PT Sans', sans-serif;\" start=\"1\" type=\"1\"><li><span style=\"font-family: Calibri, sans-serif; font-size: 12pt;\">Paraffin sections are placed at 60<sup>o<\/sup>C for 2 hours to overnight to melt paraffin.<\/span><\/li><li><span style=\"font-family: Calibri, sans-serif; font-size: 12pt;\">Paraffin sections are then placed in xylene 3X for 10 minutes each, followed by 3X in 100% ETOH for 3 minutes each, 95% ETOH for 3 minutes each, and 70% ETOH for 3 minutes each. Slides are then placed in 1X PBS for 5 minutes to completely rehydrate tissue.<\/span><\/li><li><span style=\"font-family: Calibri, sans-serif; font-size: 12pt;\">Antigen retrieval, pH 6.0 (times will vary according to microwave).<\/span><\/li><li><span style=\"font-family: Calibri, sans-serif; font-size: 12pt;\">10 mM sodium citrate, pH 6.0, and heat in a microwave at 96<sup>o<\/sup>C.\u00a0*We usually do a series of three runs (6-7 minutes each run) to equal the time\/temp because of evaporation (refill coplin jars with dH<sub>2<\/sub>O).<\/span><\/li><li><span style=\"font-family: Calibri, sans-serif; font-size: 12pt;\">Microwave according to instructions on microwave.<\/span><\/li><li><span style=\"font-family: Calibri, sans-serif; font-size: 12pt;\">Cool on countertop, 15 min.<\/span><\/li><li><span style=\"font-family: Calibri, sans-serif; font-size: 12pt;\">Rinse with dH<sub>2<\/sub>O<\/span><\/li><li><span style=\"font-family: Calibri, sans-serif; font-size: 12pt;\">1X PBS, 5 min.<\/span><\/li><li><span style=\"font-family: Calibri, sans-serif; font-size: 12pt;\">Block in 4% Goat serum\/PBS-T, 2 hours at RT.<\/span><\/li><li><span style=\"font-size: 12pt;\"><span style=\"font-family: Calibri, sans-serif;\">For Rabbit\u00a0<span style=\"font-weight: bold;\">SFTPC<\/span><\/span><span style=\"font-family: Calibri, sans-serif;\">\u00a0(RB 458 in House, BL 3-23-11, pro-SFTPC) dilute 1:500, for guinea pig anti-SFTPB (GP20, in house, mature SFTPB) dilute 1:100, for mouse anti-<\/span><a href=\"http:\/\/sevenhillsbioreagents.com\/collections\/antibodies\/products\/anti-abca3-mouse-17-h5-24\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: bold;\"><span style=\"font-family: Calibri, sans-serif;\">ABCA3<\/span><\/span><\/a><span style=\"font-family: Calibri, sans-serif;\">\u00a0(<\/span><a href=\"http:\/\/sevenhillsbioreagents.com\/collections\/antibodies\/products\/anti-abca3-mouse-17-h5-24\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: bold;\"><span style=\"font-family: Calibri, sans-serif;\">WMAB-17G524<\/span><\/span><\/a><span style=\"font-family: Calibri, sans-serif;\">, Seven Hills Bioreagents) dilute 1:100 in blocking buffer. Spin down in \u00b5fuge for 10 minutes and incubate on tissue overnight @ 4<sup>o<\/sup>C.<\/span><\/span><\/li><\/ol><div style=\"font-family: 'PT Sans', sans-serif;\"><span style=\"font-family: Calibri, sans-serif;\"><span style=\"font-size: 16px;\">\u00a0<\/span><\/span><span style=\"font-family: Calibri, sans-serif; font-weight: bold; font-size: 12pt; letter-spacing: 0px;\">Day 2<\/span><\/div><ol style=\"font-family: 'PT Sans', sans-serif;\" start=\"1\" type=\"1\"><li><span style=\"font-family: Calibri, sans-serif; font-size: 12pt;\">Rinse slides in PBS-T 3X, 5 min.<\/span><\/li><li><span style=\"font-size: 12pt;\"><span style=\"font-family: Calibri, sans-serif;\">Apply secondary antibody, Goat Alexa Fluor 488 anti-rabbit IgG (<\/span><a href=\"https:\/\/www.lifetechnologies.com\/order\/catalog\/product\/A11034?ICID=search-a11034\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: bold;\"><span style=\"font-family: Calibri, sans-serif;\">A11034<\/span><\/span><\/a><span style=\"font-family: Calibri, sans-serif;\">, Lot# 1298480,\u00a0 for anti-SFTPC),\u00a0<span style=\"color: #010101;\">Goat Alexa Fluor 555 anti-guinea pig IgG (<\/span><\/span><a href=\"http:\/\/www.lifetechnologies.com\/order\/catalog\/product\/A21435?ICID=search-a21435\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: bold;\"><span style=\"font-family: Calibri, sans-serif;\">A21435<\/span><\/span><\/a><span style=\"font-family: Calibri, sans-serif; color: #010101;\">, Lot# 1571699 for anti-SFTPB)<\/span><span style=\"font-family: Calibri, sans-serif;\">, Donkey Alexa Fluor 647 anti-mouse IgG\u00a0<\/span><a href=\"https:\/\/www.lifetechnologies.com\/order\/catalog\/product\/A31571?ICID=search-a31571\" target=\"_blank\" rel=\"noopener\"><span style=\"font-family: Calibri, sans-serif;\">A31571<\/span><\/a><span style=\"font-family: Calibri, sans-serif;\">, Lot# 1366514, anti-<\/span><a href=\"http:\/\/sevenhillsbioreagents.com\/collections\/antibodies\/products\/anti-abca3-mouse-17-h5-24\" target=\"_blank\" rel=\"noopener\"><span style=\"font-weight: bold;\"><span style=\"font-family: Calibri, sans-serif;\">ABCA3<\/span><\/span><\/a><span style=\"font-family: Calibri, sans-serif;\">)\u00a0 at 1:200, in blocking buffer. Spin down in \u00b5fuge for 20 min, apply to tissue and incubated at room temperature for 1 hour.<\/span><\/span><\/li><li><span style=\"font-family: Calibri, sans-serif; font-size: 12pt;\">Wash in PBS-T 3X, 5 min.<\/span><\/li><li><span style=\"font-family: Calibri, sans-serif; font-size: 12pt;\">Dilute DAPI 1:2000 and apply to slides for 10 min.<\/span><\/li><li><span style=\"font-family: Calibri, sans-serif; font-size: 12pt;\">Wash in PBS-T 3X, 5 min.<\/span><\/li><li><span style=\"font-family: Calibri, sans-serif; font-size: 12pt;\">Rinse slides in 0.1M PB or TB, 3X, 5 min.<\/span><\/li><li><span style=\"font-family: Calibri, sans-serif; font-size: 12pt;\">Add 1 drop of Prolong Gold anti-fade mounting medium (P36930).<\/span><\/li><li><span style=\"font-family: Calibri, sans-serif; font-size: 12pt;\">Coverslip with Gold Seal Coverslip (Cat# 3422 Electron Microscopy Sciences, 22 X 22 mm).<\/span><\/li><li><span style=\"font-family: Calibri, sans-serif; font-size: 12pt;\">Allow Prolong Gold to cure overnight at room temp in light sealed box.<\/span><\/li><li><span style=\"font-family: Calibri, sans-serif; font-size: 12pt;\">Store slides in light sealed box @ 4<sup style=\"text-indent: -24px;\">o<\/sup><span style=\"text-indent: -24px;\">C.<\/span><\/span><\/li><\/ol><div style=\"font-family: 'PT Sans', sans-serif; text-indent: -24px;\"><p style=\"text-indent: 0px;\"><span style=\"font-size: 12pt;\"><span style=\"font-weight: bold;\"><span style=\"font-family: Calibri, sans-serif;\">Tissue Used:\u00a0 \u00a0\u00a0<\/span><\/span><\/span><\/p><p style=\"text-indent: 0px;\"><span style=\"font-weight: bold; text-indent: 0.5in; font-family: 'PT Sans', Arial, Helvetica, sans-serif; letter-spacing: 0px;\"><span style=\"font-family: Calibri, sans-serif;\">17235-B2<\/span><\/span><\/p><p style=\"text-indent: 0px;\"><span style=\"font-size: 12pt;\"><span style=\"font-weight: bold;\"><span style=\"font-family: Calibri, sans-serif;\">Age: 21 Months<\/span><\/span><span style=\"font-weight: bold; letter-spacing: 0px;\"><span style=\"font-family: Calibri, sans-serif;\">\u00a0<\/span><\/span><\/span><\/p><p style=\"text-indent: 0px;\"><span style=\"font-size: 12pt;\"><span style=\"font-weight: bold;\"><span style=\"font-family: Calibri, sans-serif;\">Appendix<\/span><\/span><span style=\"font-family: Calibri, sans-serif; font-weight: bold; letter-spacing: 0px;\">\u00a0<\/span><\/span><\/p><p style=\"text-indent: 0px;\"><span style=\"font-weight: bold; font-size: 12pt;\"><span style=\"font-family: Calibri, sans-serif;\">Antigen Retrieval Solution<\/span><\/span><\/p><p style=\"text-indent: 0px;\"><span style=\"font-family: Calibri, sans-serif; font-size: 12pt;\">18ml Solution A- 0.1M Citric Acid (Sigma, C1909) pH 2.5<\/span><\/p><p style=\"text-indent: 0px;\"><span style=\"font-family: Calibri, sans-serif; font-size: 12pt;\">82ml Reagent B- 0.1M Sodium Citrate (Sigma, S4641 ) pH 8.2<\/span><\/p><p style=\"text-indent: 0px;\"><span style=\"font-family: Calibri, sans-serif; font-size: 12pt;\">1L dH<sub>2<\/sub>O<\/span><\/p><p style=\"text-indent: 0px;\"><span style=\"font-size: 12pt;\"><span style=\"font-family: Calibri, sans-serif; letter-spacing: 0px;\">pH 6.0<\/span><span style=\"font-weight: bold; letter-spacing: 0px;\"><span style=\"font-family: Calibri, sans-serif;\">\u00a0 \u00a0 \u00a0 \u00a0\u00a0<\/span><\/span><\/span><\/p><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-1a6b9cbe elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"1a6b9cbe\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-22247c04\" data-id=\"22247c04\" 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elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-128d0a1a elementor-widget elementor-widget-sidebar\" data-id=\"128d0a1a\" data-element_type=\"widget\" data-widget_type=\"sidebar.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div id=\"search-95\" class=\"widget widget_search\">\t\t<form role=\"search\" class=\"searchform fusion-search-form  fusion-search-form-classic\" method=\"get\" action=\"https:\/\/research.cchmc.org\/lungimage\/\">\n\t\t\t<div class=\"fusion-search-form-content\">\n\n\t\t\t\t\n\t\t\t\t<div class=\"fusion-search-field search-field\">\n\t\t\t\t\t<label><span class=\"screen-reader-text\">Search for:<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<input type=\"search\" value=\"\" name=\"s\" class=\"s\" placeholder=\"Search...\" required aria-required=\"true\" aria-label=\"Search...\"\/>\n\t\t\t\t\t\t\t\t\t\t\t<\/label>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"fusion-search-button search-button\">\n\t\t\t\t\t<input type=\"submit\" class=\"fusion-search-submit searchsubmit\" aria-label=\"Search\" value=\"&#xf002;\" \/>\n\t\t\t\t\t\t\t\t\t<\/div>\n\n\t\t\t\t\n\t\t\t<\/div>\n\n\n\t\t\t\n\t\t<\/form>\n\t\t<\/div><div id=\"text-84\" class=\"widget widget_text\"><div class=\"heading\"><h4 class=\"widget-title\">Related Experiments<\/h4><\/div>\t\t\t<div class=\"textwidget\"><div class=\"wondercarousellightbox_options\" data-skinsfoldername=\"\"  data-jsfolder=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/plugins\/wonderplugin-carousel\/engine\/\" style=\"display:none;\"><\/div>\r\n<div class=\"wonderplugincarousel-container\" id=\"wonderplugincarousel-container-696\"><div class=\"wonderplugincarousel\" id=\"wonderplugincarousel-696\" data-carouselid=\"696\" data-width=\"250\" data-height=\"141\" data-skin=\"vertical\" data-showimgtitle=\"false\" data-sameheight=\"false\" data-sameheightresponsive=\"false\" data-fullwidth=\"false\" data-centerimage=\"false\" data-fitimage=\"false\" data-fitcenterimage=\"false\" data-fixaspectratio=\"false\" data-autoplay=\"true\" data-random=\"false\" data-circular=\"false\" data-pauseonmouseover=\"true\" data-continuous=\"false\" data-responsive=\"true\" data-showhoveroverlay=\"true\" data-showhoveroverlayalways=\"false\" data-hidehoveroverlayontouch=\"false\" data-lightboxresponsive=\"true\" data-lightboxshownavigation=\"false\" data-lightboxnogroup=\"false\" data-lightboxshowtitle=\"true\" data-lightboxshowdescription=\"false\" data-lightboxaddsocialmedia=\"false\" data-usescreenquery=\"false\" data-donotinit=\"false\" data-addinitscript=\"false\" data-doshortcodeontext=\"false\" data-lightboxshowsocial=\"false\" data-lightboxshowfacebook=\"true\" data-lightboxshowtwitter=\"true\" data-lightboxshowpinterest=\"true\" data-lightboxsocialrotateeffect=\"true\" data-donotcircularforless=\"false\" data-hidecontainerbeforeloaded=\"false\" data-hidecontaineroninit=\"true\" data-lightboximagekeepratio=\"true\" data-showplayvideo=\"true\" data-triggerresize=\"false\" data-lightboxfullscreenmode=\"false\" data-lightboxcloseonoverlay=\"true\" data-lightboxvideohidecontrols=\"false\" data-lightboxautoslide=\"false\" data-lightboxshowtimer=\"true\" data-lightboxshowplaybutton=\"true\" data-lightboxalwaysshownavarrows=\"false\" data-lightboxshowtitleprefix=\"true\" data-spacing=\"12\" data-rownumber=\"1\" data-visibleitems=\"5\" data-arrowstyle=\"always\" data-arrowimage=\"arrows-32-32-4.png\" data-arrowwidth=\"32\" data-arrowheight=\"32\" data-navstyle=\"none\" data-navimage=\"bullet-24-24-0.png\" data-navwidth=\"24\" data-navheight=\"24\" data-navspacing=\"4\" data-hoveroverlayimage=\"hoveroverlay-64-64-4.png\" data-lightboxthumbwidth=\"90\" data-lightboxthumbheight=\"60\" data-lightboxthumbtopmargin=\"12\" data-lightboxthumbbottommargin=\"4\" data-lightboxbarheight=\"64\" data-lightboxtitlebottomcss=\"{color:#002e9b; font-size:14px; font-family:Armata,sans-serif,Arial; overflow:hidden; text-align:center;}\" data-lightboxdescriptionbottomcss=\"{color:#333; font-size:12px; font-family:Arial,Helvetica,sans-serif; overflow:hidden; text-align:left; margin:4px 0px 0px; padding: 0px;}\" data-continuousduration=\"2500\" data-autoplaydir=\"left\" data-scrollmode=\"page\" data-interval=\"3000\" data-transitionduration=\"1000\" data-lightboxtitlestyle=\"bottom\" data-lightboximagepercentage=\"75\" data-lightboxdefaultvideovolume=\"1\" data-lightboxoverlaybgcolor=\"#000\" data-lightboxoverlayopacity=\"0.9\" data-lightboxbgcolor=\"#fff\" data-lightboxtitleprefix=\"%NUM \/ %TOTAL\" data-lightboxtitleinsidecss=\"color:#fff; font-size:16px; font-family:Arial,Helvetica,sans-serif; overflow:hidden; text-align:left;\" data-lightboxdescriptioninsidecss=\"color:#fff; font-size:12px; font-family:Arial,Helvetica,sans-serif; overflow:hidden; text-align:left; margin:4px 0px 0px; padding: 0px;\" data-playvideoimage=\"playvideo-64-64-0.png\" data-playvideoimagepos=\"center\" data-imgtitle=\"title\" data-circularlimit=\"3\" data-lightboxsocialposition=\"position:absolute;top:100%;right:0;\" data-lightboxsocialpositionsmallscreen=\"position:absolute;top:100%;right:0;left:0;\" data-lightboxsocialdirection=\"horizontal\" data-lightboxsocialbuttonsize=\"32\" data-lightboxsocialbuttonfontsize=\"18\" data-sameheightmediumscreen=\"769\" data-sameheightmediumheight=\"200\" data-sameheightsmallscreen=\"415\" data-sameheightsmallheight=\"150\" data-triggerresizedelay=\"100\" data-lightboxslideinterval=\"5000\" data-lightboxtimerposition=\"bottom\" data-lightboxtimercolor=\"#dc572e\" data-lightboxtimeropacity=\"1\" data-lightboxbordersize=\"8\" data-lightboxborderradius=\"0\" data-screenquery='{ \"mobile\": { \"screenwidth\": 600, \"visibleitems\": 1 } }' data-jsfolder=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/plugins\/wonderplugin-carousel\/engine\/\" style=\"display:none;position:relative;margin:0 auto;width:100%;max-width:250px;\" ><div class=\"amazingcarousel-list-container\" style=\"overflow:hidden;\"><ul class=\"amazingcarousel-list\"><li class=\"amazingcarousel-item\"><div class=\"amazingcarousel-item-container\"><div class=\"amazingcarousel-image\">\n\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=24759\" target=\"_blank\"><img decoding=\"async\" class=\"lazyload amazingcarousel-image-img\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27480%27%20height%3D%27360%27%20viewBox%3D%270%200%20480%20360%27%3E%3Crect%20width%3D%27480%27%20height%3D%27360%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/07\/0.jpg\" alt=\"4 Month D075 SFTPB and ABCA3\" data-description=\"\" \/><\/a>\n\t<div class=\"amazingcarousel-text\">\n\t\t<div class=\"amazingcarousel-text-bg\"><\/div>\n\t\t<div class=\"amazingcarousel-title\">4 Month D075 SFTPB and ABCA3<\/div>\n\t<\/div>\n<\/div><\/div><\/li><li class=\"amazingcarousel-item\"><div class=\"amazingcarousel-item-container\"><div class=\"amazingcarousel-image\">\n\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=24992\" target=\"_blank\"><img decoding=\"async\" class=\"lazyload amazingcarousel-image-img\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27480%27%20height%3D%27360%27%20viewBox%3D%270%200%20480%20360%27%3E%3Crect%20width%3D%27480%27%20height%3D%27360%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/07\/D043.jpg\" alt=\"20 Month ABCA3 Homozygous Missense Mutation\" data-description=\"\" \/><\/a>\n\t<div class=\"amazingcarousel-text\">\n\t\t<div class=\"amazingcarousel-text-bg\"><\/div>\n\t\t<div class=\"amazingcarousel-title\">20 Month ABCA3 Homozygous Missense Mutation<\/div>\n\t<\/div>\n<\/div><\/div><\/li><li class=\"amazingcarousel-item\"><div class=\"amazingcarousel-item-container\"><div class=\"amazingcarousel-image\">\n\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=24750\" target=\"_blank\"><img decoding=\"async\" class=\"lazyload amazingcarousel-image-img\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27480%27%20height%3D%27360%27%20viewBox%3D%270%200%20480%20360%27%3E%3Crect%20width%3D%27480%27%20height%3D%27360%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/07\/8-year-Old.jpg\" alt=\"8 Year D036 SFTPB and ABCA3\" data-description=\"\" \/><\/a>\n\t<div class=\"amazingcarousel-text\">\n\t\t<div class=\"amazingcarousel-text-bg\"><\/div>\n\t\t<div class=\"amazingcarousel-title\">8 Year D036 SFTPB and ABCA3<\/div>\n\t<\/div>\n<\/div><\/div><\/li><li class=\"amazingcarousel-item\"><div class=\"amazingcarousel-item-container\"><div class=\"amazingcarousel-image\">\n\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=24826\" target=\"_blank\"><img decoding=\"async\" class=\"lazyload amazingcarousel-image-img\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27480%27%20height%3D%27360%27%20viewBox%3D%270%200%20480%20360%27%3E%3Crect%20width%3D%27480%27%20height%3D%27360%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/07\/5Month.jpg\" alt=\"5 Month ABCA3 Homozygous Missense Mutation\" data-description=\"\" \/><\/a>\n\t<div class=\"amazingcarousel-text\">\n\t\t<div class=\"amazingcarousel-text-bg\"><\/div>\n\t\t<div class=\"amazingcarousel-title\">5 Month ABCA3 Homozygous Missense Mutation<\/div>\n\t<\/div>\n<\/div><\/div><\/li><li class=\"amazingcarousel-item\"><div class=\"amazingcarousel-item-container\"><div class=\"amazingcarousel-image\">\n\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=24825\" target=\"_blank\"><img decoding=\"async\" class=\"lazyload amazingcarousel-image-img\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27480%27%20height%3D%27360%27%20viewBox%3D%270%200%20480%20360%27%3E%3Crect%20width%3D%27480%27%20height%3D%27360%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/07\/21-Month.jpg\" alt=\"21 Month ABCA3 Homozygous Missense Mutation\" data-description=\"\" \/><\/a>\n\t<div class=\"amazingcarousel-text\">\n\t\t<div class=\"amazingcarousel-text-bg\"><\/div>\n\t\t<div class=\"amazingcarousel-title\">21 Month ABCA3 Homozygous Missense Mutation<\/div>\n\t<\/div>\n<\/div><\/div><\/li><\/ul><div class=\"amazingcarousel-prev\"><\/div><div class=\"amazingcarousel-next\"><\/div><\/div><div class=\"amazingcarousel-nav\"><\/div><\/div><\/div><script>function 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id=\"recent-posts-93\" class=\"widget widget_recent_entries\">\n\t\t<div class=\"heading\"><h4 class=\"widget-title\">Recent Experiments<\/h4><\/div>\n\t\t<ul>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=32066\">4 Year-Old Human Lung KRT14, MUC5B, and TUBA4A Confocal Imaging<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31223\">Alcian Blue Staining from PBR 3653 Patient with Cystic Fibrosis<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31202\">H&#038;E Staining from PBR 3383 Patient with Cystic Fibrosis<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31199\">H&#038;E Staining from PBR 3653 Patient with Cystic Fibrosis<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31196\">H&#038;E Staining of 31 Year-Old Human Donor 10<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31193\">H&#038;E Staining of 27 Year-Old Human Donor 3<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31190\">Alcian Blue Staining of 27 Year-Old Human Donor 3<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31186\">Alcian Blue Staining of 31 Year-Old Human Donor 10<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31182\">Alcian Blue Staining from PBR 3383 Patient with Cystic Fibrosis<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31024\">Alcian Blue Staining of 29 Year-Old Human Donor D175-10C1.21<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31019\">Alcian Blue Staining of 29 Year-Old Human Donor D175-11C4.14<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31016\">Alcian Blue Staining of 29 Year-Old Human Donor D175-11C2.11<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31013\">Alcian Blue Staining of 24 Year-Old Human Donor D071-23C2.19<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31010\">Alcian Blue Staining of 24 Year-Old Human Donor D071-17C2.20<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31007\">Alcian Blue Staining of 24 Year-Old Human Donor D071-16C3.27<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t<\/ul>\n\n\t\t<\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>\u00a0 Immunofluorescence- SFTPC, SFTPB , and\u00a0ABCA3 \u00a0 Purpose:\u00a0\u00a0Stain slides of 5\u00b5m paraffin sections of 21 months-old postnatal human lung from HTC\u00a0SFTPC, SFTPB, andABCA3\u00a0with antigen retrieval. Day 1 Paraffin sections are placed at 60oC for 2 hours to overnight to melt paraffin. Paraffin sections are then placed in xylene 3X for 10 minutes each, followed by [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":24831,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-24825","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-uncategorized"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v25.6 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>21 Month-Old Human Lung Enhanced Resolution Confocal Imaging From Donor 17235-B2 containing an ABCA3 Homozygous Missense Mutation Showing Expression of SFTPB and ABCA3 -<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/research.cchmc.org\/lungimage\/?p=24825\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"21 Month-Old Human Lung Enhanced Resolution Confocal Imaging From Donor 17235-B2 containing an ABCA3 Homozygous Missense Mutation Showing Expression of SFTPB and ABCA3 -\" \/>\n<meta property=\"og:description\" content=\"\u00a0 Immunofluorescence- SFTPC, SFTPB , and\u00a0ABCA3 \u00a0 Purpose:\u00a0\u00a0Stain slides of 5\u00b5m paraffin sections of 21 months-old postnatal human lung from HTC\u00a0SFTPC, SFTPB, andABCA3\u00a0with antigen retrieval. 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