{"id":27446,"date":"2018-09-19T18:22:48","date_gmt":"2018-09-19T18:22:48","guid":{"rendered":"https:\/\/research.cchmc.org\/lungimage\/?p=27446"},"modified":"2019-09-10T19:25:33","modified_gmt":"2019-09-10T19:25:33","slug":"he-staining-of-11-month-old-human-lung-from-donor-d094-lll-5b2","status":"publish","type":"post","link":"https:\/\/research.cchmc.org\/lungimage\/?p=27446","title":{"rendered":"H&#038;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"27446\" class=\"elementor elementor-27446\" data-elementor-post-type=\"post\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-2974d3f4 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"2974d3f4\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-4c18e27f\" data-id=\"4c18e27f\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-1fb5e2f2 elementor-widget elementor-widget-theme-post-title elementor-page-title elementor-widget-heading\" data-id=\"1fb5e2f2\" data-element_type=\"widget\" data-widget_type=\"theme-post-title.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h1 class=\"elementor-heading-title elementor-size-large\">H&#038;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-718e5427 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"718e5427\" 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data-thumbmediumheight=\"64\" data-thumbsmallwidth=\"48\" data-thumbsmallheight=\"48\" data-imgtitle=\"title\" data-jsfolder=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/plugins\/wonderplugin-gallery\/engine\/\" style=\"display:none;\" ><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_4X_HE_LMH-18-D094-5B2.11_001_TileScan_Crop.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271120%27%20height%3D%271024%27%20viewBox%3D%270%200%201120%201024%27%3E%3Crect%20width%3D%271120%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_4X_HE_LMH-18-D094-5B2.11_001_TileScan_Crop.jpg\" alt=\"H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2\" data-description=\"4X Tile Scan\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2<\/div><div class=\"html5gallery-description\">4X Tile Scan<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_4X_HE_LMH-18-D094-5B2.11_001.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_4X_HE_LMH-18-D094-5B2.11_001.jpg\" alt=\"H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2\" data-description=\"4X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2<\/div><div class=\"html5gallery-description\">4X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_10X_HE_LMH-18-D094-5B2.11_001.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_10X_HE_LMH-18-D094-5B2.11_001.jpg\" alt=\"H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2\" data-description=\"10X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2<\/div><div class=\"html5gallery-description\">10X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_20X_HE_LMH-18-D094-5B2.11_001.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_20X_HE_LMH-18-D094-5B2.11_001.jpg\" alt=\"H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_20X_HE_LMH-18-D094-5B2.11_001-1.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_20X_HE_LMH-18-D094-5B2.11_001-1.jpg\" alt=\"H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_10X_HE_LMH-18-D094-5B2.11_001a.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_10X_HE_LMH-18-D094-5B2.11_001a.jpg\" alt=\"H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2\" data-description=\"10X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2<\/div><div class=\"html5gallery-description\">10X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_20X_HE_LMH-18-D094-5B2.11_001a.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_20X_HE_LMH-18-D094-5B2.11_001a.jpg\" alt=\"H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_20X_HE_LMH-18-D094-5B2.11_001a-1.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_20X_HE_LMH-18-D094-5B2.11_001a-1.jpg\" alt=\"H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_4X_HE_LMH-18-D094-5B2.11_002.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_4X_HE_LMH-18-D094-5B2.11_002.jpg\" alt=\"H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2\" data-description=\"4X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2<\/div><div class=\"html5gallery-description\">4X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_10X_HE_LMH-18-D094-5B2.11_002.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_10X_HE_LMH-18-D094-5B2.11_002.jpg\" alt=\"H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2\" data-description=\"10X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2<\/div><div class=\"html5gallery-description\">10X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_20X_HE_LMH-18-D094-5B2.11_002.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_20X_HE_LMH-18-D094-5B2.11_002.jpg\" alt=\"H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_20X_HE_LMH-18-D094-5B2.11_002-1.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_20X_HE_LMH-18-D094-5B2.11_002-1.jpg\" alt=\"H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_10X_HE_LMH-18-D094-5B2.11_002a.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_10X_HE_LMH-18-D094-5B2.11_002a.jpg\" alt=\"H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2\" data-description=\"10X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2<\/div><div class=\"html5gallery-description\">10X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_20X_HE_LMH-18-D094-5B2.11_002a.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_20X_HE_LMH-18-D094-5B2.11_002a.jpg\" alt=\"H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_20X_HE_LMH-18-D094-5B2.11_002a-1.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_20X_HE_LMH-18-D094-5B2.11_002a-1.jpg\" alt=\"H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_4X_HE_LMH-18-D094-5B2.11_003.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_4X_HE_LMH-18-D094-5B2.11_003.jpg\" alt=\"H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2\" data-description=\"4X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2<\/div><div class=\"html5gallery-description\">4X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_10X_HE_LMH-18-D094-5B2.11_003.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_10X_HE_LMH-18-D094-5B2.11_003.jpg\" alt=\"H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2\" data-description=\"10X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2<\/div><div class=\"html5gallery-description\">10X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_20X_HE_LMH-18-D094-5B2.11_003.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_20X_HE_LMH-18-D094-5B2.11_003.jpg\" alt=\"H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_20X_HE_LMH-18-D094-5B2.11_003-1.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_20X_HE_LMH-18-D094-5B2.11_003-1.jpg\" alt=\"H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_10X_HE_LMH-18-D094-5B2.11_003a.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_10X_HE_LMH-18-D094-5B2.11_003a.jpg\" alt=\"H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2\" data-description=\"10X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2<\/div><div class=\"html5gallery-description\">10X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_20X_HE_LMH-18-D094-5B2.11_003a.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_20X_HE_LMH-18-D094-5B2.11_003a.jpg\" alt=\"H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_20X_HE_LMH-18-D094-5B2.11_003a-1.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_20X_HE_LMH-18-D094-5B2.11_003a-1.jpg\" alt=\"H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_4X_HE_LMH-18-D094-5B2.11_004.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_4X_HE_LMH-18-D094-5B2.11_004.jpg\" alt=\"H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2\" data-description=\"4X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2<\/div><div class=\"html5gallery-description\">4X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_10X_HE_LMH-18-D094-5B2.11_004.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_10X_HE_LMH-18-D094-5B2.11_004.jpg\" alt=\"H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2\" data-description=\"10X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2<\/div><div class=\"html5gallery-description\">10X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_20X_HE_LMH-18-D094-5B2.11_004.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_20X_HE_LMH-18-D094-5B2.11_004.jpg\" alt=\"H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_20X_HE_LMH-18-D094-5B2.11_004-1.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_20X_HE_LMH-18-D094-5B2.11_004-1.jpg\" alt=\"H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_10X_HE_LMH-18-D094-5B2.11_004a.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_10X_HE_LMH-18-D094-5B2.11_004a.jpg\" alt=\"H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2\" data-description=\"10X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2<\/div><div class=\"html5gallery-description\">10X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_20X_HE_LMH-18-D094-5B2.11_004a.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_20X_HE_LMH-18-D094-5B2.11_004a.jpg\" alt=\"H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_20X_HE_LMH-18-D094-5B2.11_004a-1.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/09\/2018-09-17_20X_HE_LMH-18-D094-5B2.11_004a-1.jpg\" alt=\"H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><\/div><\/div><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-57cc66bd elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"57cc66bd\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-587de568\" data-id=\"587de568\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-66244fb4 elementor-widget elementor-widget-text-editor\" data-id=\"66244fb4\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>\u00a0\u00a0Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from\u00a0the University of Rochester Medical Center<\/p><p><a href=\"https:\/\/www.urmc.rochester.edu\/labs\/Pryhuber-Lab\/\" target=\"_blank\" rel=\"noopener noreferrer\"><img class=\"lazyload\" decoding=\"async\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27200%27%20height%3D%2748%27%20viewBox%3D%270%200%20200%2048%27%3E%3Crect%20width%3D%27200%27%20height%3D%2748%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2015\/07\/Pryhuber_Lab.jpg\" alt=\"Pryhuber_Lab\" width=\"200\" height=\"48\" \/><\/a><\/p><ol><li>For Frozen tissue, rinsed slides for 2X in PBS, then Incubated slides for 5 min in 4% PFA\/PBS, then rinsed slides 1X in PBS.<\/li><li>Rinsed slides for 3 minutes in running dH<sub>2<\/sub>O.<\/li><li>Slides were then stained in filtered Hematoxylin for 60 seconds. (Gold Standard Harris Hemotoxylin, Acidified, Mercury; Cat # 24245 Polysciences Inc,).<\/li><li>Rinsed slides in tap water until water turned cleared then rinsed in dH<sub>2<\/sub>O<\/li><li>Rinsed slides in Lithium Carbonate: 20 dips. (Lithium Carbonate ACS Grade; Cat # 255823-500G Sigma Aldrich). Lithium carbonate is prepared by over-saturating in dH<sub>2<\/sub>O.<\/li><li>Rinsed slides in dH<sub>2<\/sub>O 3X 20 dips.<\/li><li>Rinsed slides in 70% ETOH for 20 dips.<\/li><li>Rinsed slides in 80% ETOH for 20 dips.<\/li><li>Stained slides in Eosin for 15 seconds. (Eosin Y 0.5% Alcohol Solution, Cat # 09859 Polysciences Inc,).<\/li><li>Rinsed slides in a series of 95% ETOH washes. 10 dips for the first and second washes, 15 dips in the third and fourth washes.<\/li><li>Rinsed slides 3X in 100% ETOH. 10 dips for the first and second washes and 15 dips in the third and fourth washes.<\/li><li>Rinsed slides in 2X in Xylene, followed by 6 more washes in xylene.<\/li><li>Coverslip with Permount. (Permount Toulene Solution UN 1294, Cat # SP-15-500 Fisher Scientific).<\/li><\/ol><p><strong>\u00a0<\/strong><\/p><p><strong>Tissue Used:<br \/><\/strong><strong style=\"letter-spacing: 0px;\">LMH-D094-LLL-5B2.11<br \/><\/strong><strong style=\"letter-spacing: 0px;\">Gender: Male<br \/><\/strong><strong style=\"letter-spacing: 0px;\">Age: 11 Months<\/strong><\/p><p>\u00a0<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-2284959b elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"2284959b\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-2b6fb4e\" data-id=\"2b6fb4e\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-bd9d399 elementor-widget elementor-widget-facebook-comments\" data-id=\"bd9d399\" data-element_type=\"widget\" data-widget_type=\"facebook-comments.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-facebook-widget fb-comments\" data-href=\"https:\/\/research.cchmc.org\/lungimage?p=27446\" data-width=\"100%\" data-numposts=\"10\" data-order-by=\"social\" style=\"min-height: 1px\"><\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-10302fc8\" data-id=\"10302fc8\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-163b5a6d elementor-widget elementor-widget-sidebar\" data-id=\"163b5a6d\" data-element_type=\"widget\" data-widget_type=\"sidebar.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\n\t\t<div id=\"recent-posts-99\" class=\"widget widget_recent_entries\">\n\t\t<div class=\"heading\"><h4 class=\"widget-title\">Recent Experiments<\/h4><\/div>\n\t\t<ul>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=32066\">4 Year-Old Human Lung KRT14, MUC5B, and TUBA4A Confocal Imaging<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31223\">Alcian Blue Staining from PBR 3653 Patient with Cystic Fibrosis<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31202\">H&#038;E Staining from PBR 3383 Patient with Cystic Fibrosis<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31199\">H&#038;E Staining from PBR 3653 Patient with Cystic Fibrosis<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31196\">H&#038;E Staining of 31 Year-Old Human Donor 10<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31193\">H&#038;E Staining of 27 Year-Old Human Donor 3<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31190\">Alcian Blue Staining of 27 Year-Old Human Donor 3<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31186\">Alcian Blue Staining of 31 Year-Old Human Donor 10<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31182\">Alcian Blue Staining from PBR 3383 Patient with Cystic Fibrosis<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31024\">Alcian Blue Staining of 29 Year-Old Human Donor D175-10C1.21<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31019\">Alcian Blue Staining of 29 Year-Old Human Donor D175-11C4.14<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31016\">Alcian Blue Staining of 29 Year-Old Human Donor D175-11C2.11<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31013\">Alcian Blue Staining of 24 Year-Old Human Donor D071-23C2.19<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31010\">Alcian Blue Staining of 24 Year-Old Human Donor D071-17C2.20<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31007\">Alcian Blue Staining of 24 Year-Old Human Donor D071-16C3.27<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t<\/ul>\n\n\t\t<\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>\u00a0\u00a0Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from\u00a0the University of Rochester Medical Center For Frozen tissue, rinsed slides for 2X in PBS, then Incubated slides for 5 min in 4% PFA\/PBS, then rinsed slides 1X in PBS. Rinsed slides for 3 minutes in running dH2O. Slides were then stained in filtered Hematoxylin for 60 [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":27445,"comment_status":"closed","ping_status":"open","sticky":false,"template":"elementor_header_footer","format":"standard","meta":{"footnotes":""},"categories":[72,77],"tags":[],"class_list":["post-27446","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-he","category-perinatalhuman-he"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v25.6 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2 -<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/research.cchmc.org\/lungimage\/?p=27446\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"H&amp;E Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2 -\" \/>\n<meta property=\"og:description\" content=\"\u00a0\u00a0Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from\u00a0the University of Rochester Medical Center For Frozen tissue, rinsed slides for 2X in PBS, then Incubated slides for 5 min in 4% PFA\/PBS, then rinsed slides 1X in PBS. 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