{"id":27865,"date":"2018-10-02T17:29:27","date_gmt":"2018-10-02T17:29:27","guid":{"rendered":"https:\/\/research.cchmc.org\/lungimage\/?p=27865"},"modified":"2019-09-06T19:16:36","modified_gmt":"2019-09-06T19:16:36","slug":"he-staining-of-14-month-old-human-lung-from-donor-d072-rll-14b2","status":"publish","type":"post","link":"https:\/\/research.cchmc.org\/lungimage\/?p=27865","title":{"rendered":"H&E Staining of 14 Month-Old Human Lung from Donor D072-RLL-14B2"},"content":{"rendered":"\t\t
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H&E Staining of 14 Month-Old Human Lung from Donor D072-RLL-14B2<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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\"H&E<\/a>
H&E Staining of 14 Month-Old Human Lung from Donor D072-RLL-14B2<\/div>
4X Tile Scan<\/div><\/div>\"H&E<\/a>
H&E Staining of 14 Month-Old Human Lung from Donor D072-RLL-14B2<\/div>
4X<\/div><\/div>\"H&E<\/a>
H&E Staining of 14 Month-Old Human Lung from Donor D072-RLL-14B2<\/div>
10X<\/div><\/div>\"H&E<\/a>
H&E Staining of 14 Month-Old Human Lung from Donor D072-RLL-14B2<\/div>
20X<\/div><\/div>\"H&E<\/a>
H&E Staining of 14 Month-Old Human Lung from Donor D072-RLL-14B2<\/div>
20X<\/div><\/div>\"H&E<\/a>
H&E Staining of 14 Month-Old Human Lung from Donor D072-RLL-14B2<\/div>
10X<\/div><\/div>\"H&E<\/a>
H&E Staining of 14 Month-Old Human Lung from Donor D072-RLL-14B2<\/div>
20X<\/div><\/div>\"H&E<\/a>
H&E Staining of 14 Month-Old Human Lung from Donor D072-RLL-14B2<\/div>
20X<\/div><\/div>\"H&E<\/a>
H&E Staining of 14 Month-Old Human Lung from Donor D072-RLL-14B2<\/div>
4X<\/div><\/div>\"H&E<\/a>
H&E Staining of 14 Month-Old Human Lung from Donor D072-RLL-14B2<\/div>
10X<\/div><\/div>\"H&E<\/a>
H&E Staining of 14 Month-Old Human Lung from Donor D072-RLL-14B2<\/div>
20X<\/div><\/div>\"H&E<\/a>
H&E Staining of 14 Month-Old Human Lung from Donor D072-RLL-14B2<\/div>
20X<\/div><\/div>\"H&E<\/a>
H&E Staining of 14 Month-Old Human Lung from Donor D072-RLL-14B2<\/div>
10X<\/div><\/div>\"H&E<\/a>
H&E Staining of 14 Month-Old Human Lung from Donor D072-RLL-14B2<\/div>
20X<\/div><\/div>\"H&E<\/a>
H&E Staining of 14 Month-Old Human Lung from Donor D072-RLL-14B2<\/div>
20X<\/div><\/div>\"H&E<\/a>
H&E Staining of 14 Month-Old Human Lung from Donor D072-RLL-14B2<\/div>
4X<\/div><\/div>\"H&E<\/a>
H&E Staining of 14 Month-Old Human Lung from Donor D072-RLL-14B2<\/div>
10X<\/div><\/div>\"H&E<\/a>
H&E Staining of 14 Month-Old Human Lung from Donor D072-RLL-14B2<\/div>
20X<\/div><\/div>\"H&E<\/a>
H&E Staining of 14 Month-Old Human Lung from Donor D072-RLL-14B2<\/div>
20X<\/div><\/div>\"H&E<\/a>
H&E Staining of 14 Month-Old Human Lung from Donor D072-RLL-14B2<\/div>
10X<\/div><\/div>\"H&E<\/a>
H&E Staining of 14 Month-Old Human Lung from Donor D072-RLL-14B2<\/div>
20X<\/div><\/div>\"H&E<\/a>
H&E Staining of 14 Month-Old Human Lung from Donor D072-RLL-14B2<\/div>
20X<\/div><\/div>\"H&E<\/a>
H&E Staining of 14 Month-Old Human Lung from Donor D072-RLL-14B2<\/div>
4X<\/div><\/div>\"H&E<\/a>
H&E Staining of 14 Month-Old Human Lung from Donor D072-RLL-14B2<\/div>
10X<\/div><\/div>\"H&E<\/a>
H&E Staining of 14 Month-Old Human Lung from Donor D072-RLL-14B2<\/div>
20X<\/div><\/div>\"H&E<\/a>
H&E Staining of 14 Month-Old Human Lung from Donor D072-RLL-14B2<\/div>
20X<\/div><\/div>\"H&E<\/a>
H&E Staining of 14 Month-Old Human Lung from Donor D072-RLL-14B2<\/div>
10X<\/div><\/div>\"H&E<\/a>
H&E Staining of 14 Month-Old Human Lung from Donor D072-RLL-14B2<\/div>
20X<\/div><\/div>\"H&E<\/a>
H&E Staining of 14 Month-Old Human Lung from Donor D072-RLL-14B2<\/div>
20X<\/div><\/div><\/div><\/div><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from\u00a0the University of Rochester Medical Center<\/p>

\"Pryhuber_Lab\"<\/a><\/p>

  1. Paraffin sections were placed at 60o<\/sup>C overnight to melt paraffin.<\/li>
  2. Paraffin sections were then placed in xylene (Xylenes Reagent Grade ACS Histology\/Lab Grade Cat # 399000000 Pharma-AAPER) 3X for 10 minutes each.<\/li>
  3. Rinsed slides in a series of 100% ETOH washes. 20 dips for the first wash, 3 minutes for the second wash, and 20 dips for the third wash.<\/li>
  4. Rinsed slides in a series of 95% ETOH washes. 20 dips for the first wash, 3 minutes for the second wash, and 20 dips for the third wash.<\/li>
  5. Rinsed slides in 70% ETOH for 3 min.<\/li>
  6. Rinsed slides for 3 minutes in running dH2<\/sub>O.<\/li>
  7. Slides were then stained in filtered Hematoxylin for 60 seconds. (Gold Standard Harris Hemotoxylin, Acidified, Mercury; Cat # 24245 Polysciences Inc,).<\/li>
  8. Rinsed slides in tap water until water turned cleared then rinsed in dH2<\/sub>O<\/li>
  9. Rinsed slides in Lithium Carbonate: 20 dips. (Lithium Carbonate ACS Grade; Cat # 255823-500G Sigma Aldrich). Lithium carbonate is prepared by over-saturating in dH2<\/sub>O.<\/li>
  10. Rinsed slides in dH2<\/sub>O 3X 20 dips.<\/li>
  11. Rinsed slides in 70% ETOH for 20 dips.<\/li>
  12. Rinsed slides in 80% ETOH for 20 dips.<\/li>
  13. Stained slides in Eosin for 15 seconds. (Eosin Y 0.5% Alcohol Solution, Cat # 09859 Polysciences Inc,).<\/li>
  14. Rinsed slides in a series of 95% ETOH washes. 10 dips for the first and second washes, 15 dips in the third and fourth washes.<\/li>
  15. Rinsed slides 3X in 100% ETOH. 10 dips for the first and second washes and 15 dips in the third and fourth washes.<\/li>
  16. Rinsed slides in 2X in Xylene, followed by 6 more washes in xylene.<\/li>
  17. Coverslip with Permount. (Permount Toulene Solution UN 1294, Cat # SP-15-500 Fisher Scientific).<\/li><\/ol>

    \u00a0<\/span><\/p>

    Tissue Used:
    <\/span>LMH-18-D072-RLL-14B2.17
    <\/span>Gender: Female
    <\/span>Age: 14 Months<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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    Recent Experiments<\/h4><\/div>\n\t\t