{"id":28196,"date":"2018-10-12T16:31:45","date_gmt":"2018-10-12T16:31:45","guid":{"rendered":"https:\/\/research.cchmc.org\/lungimage\/?p=28196"},"modified":"2019-09-10T20:01:45","modified_gmt":"2019-09-10T20:01:45","slug":"alcain-blue-staining-of-4-month-old-human-lung-from-donor-d075-rll-10b1","status":"publish","type":"post","link":"https:\/\/research.cchmc.org\/lungimage\/?p=28196","title":{"rendered":"Alcain Blue Staining of 4 Month-Old Human Lung from Donor D075-RLL-10B1"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"28196\" class=\"elementor elementor-28196\" data-elementor-post-type=\"post\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-6af8d8bb elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"6af8d8bb\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-c23ec06\" data-id=\"c23ec06\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-2850e596 elementor-widget elementor-widget-theme-post-title elementor-page-title elementor-widget-heading\" data-id=\"2850e596\" data-element_type=\"widget\" data-widget_type=\"theme-post-title.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h1 class=\"elementor-heading-title elementor-size-large\">Alcain Blue Staining of 4 Month-Old Human Lung from Donor D075-RLL-10B1<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-2959da1d elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"2959da1d\" 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data-thumbmediumheight=\"64\" data-thumbsmallwidth=\"48\" data-thumbsmallheight=\"48\" data-imgtitle=\"title\" data-jsfolder=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/plugins\/wonderplugin-gallery\/engine\/\" style=\"display:none;\" ><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-11_4X_AB_LMH-18-D075-10B1.1_001_TileScan_Crop.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271914%27%20height%3D%271024%27%20viewBox%3D%270%200%201914%201024%27%3E%3Crect%20width%3D%271914%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-11_4X_AB_LMH-18-D075-10B1.1_001_TileScan_Crop.jpg\" alt=\"AB Staining of 4 Month-Old Human Lung from Donor D075-RLL-10B1\" data-description=\"4X Tile Scan\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">AB Staining of 4 Month-Old Human Lung from Donor D075-RLL-10B1<\/div><div class=\"html5gallery-description\">4X Tile Scan<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-11_4X_AB_LMH-18-D075-10B1.1_001.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-11_4X_AB_LMH-18-D075-10B1.1_001.jpg\" alt=\"AB Staining of 4 Month-Old Human Lung from Donor D075-RLL-10B1\" data-description=\"4X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">AB Staining of 4 Month-Old Human Lung from Donor D075-RLL-10B1<\/div><div class=\"html5gallery-description\">4X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-11_10X_AB_LMH-18-D075-10B1.1_001.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-11_10X_AB_LMH-18-D075-10B1.1_001.jpg\" alt=\"AB Staining of 4 Month-Old Human Lung from Donor D075-RLL-10B1\" data-description=\"10X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">AB Staining of 4 Month-Old Human Lung from Donor D075-RLL-10B1<\/div><div class=\"html5gallery-description\">10X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-11_20X_AB_LMH-18-D075-10B1.1_001.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-11_20X_AB_LMH-18-D075-10B1.1_001.jpg\" alt=\"AB Staining of 4 Month-Old Human Lung from Donor D075-RLL-10B1\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">AB Staining of 4 Month-Old Human Lung from Donor D075-RLL-10B1<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-11_20X_AB_LMH-18-D075-10B1.1_001-1.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-11_20X_AB_LMH-18-D075-10B1.1_001-1.jpg\" alt=\"AB Staining of 4 Month-Old Human Lung from Donor D075-RLL-10B1\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">AB Staining of 4 Month-Old Human Lung from Donor D075-RLL-10B1<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-11_10X_AB_LMH-18-D075-10B1.1_001a.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-11_10X_AB_LMH-18-D075-10B1.1_001a.jpg\" alt=\"AB Staining of 4 Month-Old Human Lung from Donor D075-RLL-10B1\" data-description=\"10X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">AB Staining of 4 Month-Old Human Lung from Donor D075-RLL-10B1<\/div><div class=\"html5gallery-description\">10X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-11_20X_AB_LMH-18-D075-10B1.1_001a.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-11_20X_AB_LMH-18-D075-10B1.1_001a.jpg\" alt=\"AB Staining of 4 Month-Old Human Lung from Donor D075-RLL-10B1\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">AB Staining of 4 Month-Old Human Lung from Donor D075-RLL-10B1<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-11_20X_AB_LMH-18-D075-10B1.1_001a-1.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-11_20X_AB_LMH-18-D075-10B1.1_001a-1.jpg\" alt=\"AB Staining of 4 Month-Old Human Lung from Donor D075-RLL-10B1\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">AB Staining of 4 Month-Old Human Lung from Donor D075-RLL-10B1<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-11_4X_AB_LMH-18-D075-10B1.1_002.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-11_4X_AB_LMH-18-D075-10B1.1_002.jpg\" alt=\"AB Staining of 4 Month-Old Human Lung from Donor D075-RLL-10B1\" data-description=\"4X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">AB Staining of 4 Month-Old Human Lung from Donor D075-RLL-10B1<\/div><div class=\"html5gallery-description\">4X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-11_10X_AB_LMH-18-D075-10B1.1_002.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-11_10X_AB_LMH-18-D075-10B1.1_002.jpg\" alt=\"AB Staining of 4 Month-Old Human Lung from Donor D075-RLL-10B1\" data-description=\"10X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">AB Staining of 4 Month-Old Human Lung from Donor D075-RLL-10B1<\/div><div class=\"html5gallery-description\">10X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-11_20X_AB_LMH-18-D075-10B1.1_002.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-11_20X_AB_LMH-18-D075-10B1.1_002.jpg\" alt=\"AB Staining of 4 Month-Old Human Lung from Donor D075-RLL-10B1\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">AB Staining of 4 Month-Old Human Lung from Donor D075-RLL-10B1<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-11_20X_AB_LMH-18-D075-10B1.1_002-1.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-11_20X_AB_LMH-18-D075-10B1.1_002-1.jpg\" alt=\"AB Staining of 4 Month-Old Human Lung from Donor D075-RLL-10B1\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">AB Staining of 4 Month-Old Human Lung from Donor D075-RLL-10B1<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-11_10X_AB_LMH-18-D075-10B1.1_002a.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-11_10X_AB_LMH-18-D075-10B1.1_002a.jpg\" alt=\"AB Staining of 4 Month-Old Human Lung from Donor D075-RLL-10B1\" data-description=\"10X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">AB Staining of 4 Month-Old Human Lung from Donor D075-RLL-10B1<\/div><div class=\"html5gallery-description\">10X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-11_20X_AB_LMH-18-D075-10B1.1_002a.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-11_20X_AB_LMH-18-D075-10B1.1_002a.jpg\" alt=\"AB Staining of 4 Month-Old Human Lung from Donor D075-RLL-10B1\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">AB Staining of 4 Month-Old Human Lung from Donor D075-RLL-10B1<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-11_20X_AB_LMH-18-D075-10B1.1_002a-1.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-11_20X_AB_LMH-18-D075-10B1.1_002a-1.jpg\" alt=\"AB Staining of 4 Month-Old Human Lung from Donor D075-RLL-10B1\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">AB Staining of 4 Month-Old Human Lung from Donor D075-RLL-10B1<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><\/div><\/div><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-691757b3 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"691757b3\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-226bcdf1\" data-id=\"226bcdf1\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-5573b806 elementor-widget elementor-widget-text-editor\" data-id=\"5573b806\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"margin: 0px 0px 20px; font-family: 'PT Sans', Arial, Helvetica, sans-serif;\">Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from\u00a0the University of Rochester Medical Center<\/p><p style=\"margin: 0px 0px 20px; font-family: 'PT Sans', Arial, Helvetica, sans-serif;\"><a href=\"https:\/\/www.urmc.rochester.edu\/labs\/Pryhuber-Lab\/\" target=\"_blank\" rel=\"noopener\"><img class=\"lazyload\" decoding=\"async\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27200%27%20height%3D%2748%27%20viewBox%3D%270%200%20200%2048%27%3E%3Crect%20width%3D%27200%27%20height%3D%2748%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2015\/07\/Pryhuber_Lab.jpg\" alt=\"Pryhuber_Lab\" width=\"200\" height=\"48\" \/><\/a><\/p><ol style=\"font-family: 'PT Sans', Arial, Helvetica, sans-serif;\"><li>Paraffin sections were placed at 60<sup>o<\/sup>C overnight to melt paraffin.<\/li><li>Paraffin sections were then placed in xylene (Xylenes Reagent Grade ACS Histology\/Lab Grade Cat # 399000000 Pharma-AAPER) 3X for 10 minutes each.<\/li><li>Rinsed slides for 3 minutes in running dH<sub>2<\/sub>O.<\/li><li>Slides were then incubated in 3% Glacial Acetic Acid (<a href=\"http:\/\/www.polyrnd.com\/acetic-acid-3-aqueous-10185.html\"><span style=\"font-weight: bold;\">S101<\/span><\/a>, Poly Scientific) for 3 minutes.<\/li><li>Slides were then incubated in filtered Alcian Blue (<a href=\"http:\/\/www.polyrnd.com\/alcian-blue-1-in-3-acetic-acid-ph-2-5-15114.html\"><span style=\"font-weight: bold;\">S111A<\/span><\/a>, Poly Scientific) for 30 minutes.<\/li><li>Wash slides under cold running tap water for 10 minutes.<\/li><li>Incubate for 2 minutes in Nuclear Fast Red (<a href=\"http:\/\/www.polyrnd.com\/nuclear-fast-red-kernechtrot-0-7.html\"><span style=\"font-weight: bold;\">S248<\/span><\/a>, Poly Scientific).<\/li><li>Wash under flowing\u00a0dH<sub>2<\/sub>O until excess Nuclear Fast Red is washed away.<\/li><li>Incubated\u00a0slides in 90% ETOH for 20 dips, repeat in different 90% ETOH.<\/li><li>Incubated slides in 100% ETOH for 20 dips, repeat in different\u00a0100% ETOH.<\/li><li>Washed\u00a0slides in 2X in Xylene, followed by 6 more washes in xylene.<\/li><li>Coverslip\u00a0with Permount. (Permount Toulene Solution UN 1294, Cat #\u00a0<a href=\"https:\/\/www.fishersci.com\/shop\/products\/fisher-chemical-permount-mounting-medium-2\/sp15500\"><span style=\"font-weight: bold;\">SP-15-500<\/span><\/a>\u00a0Fisher Scientific).<\/li><\/ol><p style=\"margin: 0px 0px 20px; font-family: 'PT Sans', Arial, Helvetica, sans-serif;\"><span style=\"font-weight: bold;\">\u00a0<\/span><\/p><p style=\"margin: 0px 0px 20px; font-family: 'PT Sans', Arial, Helvetica, sans-serif;\"><span style=\"font-weight: bold;\">Tissue Used:<br \/><\/span><span style=\"font-weight: bold; letter-spacing: 0px;\">LMH-18-D075-RLL-10B1.1<br \/><\/span><span style=\"font-weight: bold; letter-spacing: 0px;\">Gender: Male<br \/><\/span><span style=\"font-weight: bold; letter-spacing: 0px;\">Age: 4 Months<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-3062703d elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"3062703d\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-4cede525\" data-id=\"4cede525\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-320d6efc elementor-widget elementor-widget-facebook-comments\" data-id=\"320d6efc\" data-element_type=\"widget\" data-widget_type=\"facebook-comments.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-facebook-widget fb-comments\" data-href=\"https:\/\/research.cchmc.org\/lungimage?p=28196\" data-width=\"100%\" data-numposts=\"10\" data-order-by=\"social\" style=\"min-height: 1px\"><\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-728bae8d\" data-id=\"728bae8d\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-9723b0e elementor-widget elementor-widget-sidebar\" data-id=\"9723b0e\" data-element_type=\"widget\" data-widget_type=\"sidebar.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\n\t\t<div id=\"recent-posts-99\" class=\"widget widget_recent_entries\">\n\t\t<div class=\"heading\"><h4 class=\"widget-title\">Recent Experiments<\/h4><\/div>\n\t\t<ul>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=32066\">4 Year-Old Human Lung KRT14, MUC5B, and TUBA4A Confocal Imaging<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31223\">Alcian Blue Staining from PBR 3653 Patient with Cystic Fibrosis<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31202\">H&#038;E Staining from PBR 3383 Patient with Cystic Fibrosis<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31199\">H&#038;E Staining from PBR 3653 Patient with Cystic Fibrosis<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31196\">H&#038;E Staining of 31 Year-Old Human Donor 10<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31193\">H&#038;E Staining of 27 Year-Old Human Donor 3<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31190\">Alcian Blue Staining of 27 Year-Old Human Donor 3<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31186\">Alcian Blue Staining of 31 Year-Old Human Donor 10<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31182\">Alcian Blue Staining from PBR 3383 Patient with Cystic Fibrosis<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31024\">Alcian Blue Staining of 29 Year-Old Human Donor D175-10C1.21<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31019\">Alcian Blue Staining of 29 Year-Old Human Donor D175-11C4.14<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31016\">Alcian Blue Staining of 29 Year-Old Human Donor D175-11C2.11<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31013\">Alcian Blue Staining of 24 Year-Old Human Donor D071-23C2.19<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31010\">Alcian Blue Staining of 24 Year-Old Human Donor D071-17C2.20<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31007\">Alcian Blue Staining of 24 Year-Old Human Donor D071-16C3.27<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t<\/ul>\n\n\t\t<\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from\u00a0the University of Rochester Medical Center Paraffin sections were placed at 60oC overnight to melt paraffin. Paraffin sections were then placed in xylene (Xylenes Reagent Grade ACS Histology\/Lab Grade Cat # 399000000 Pharma-AAPER) 3X for 10 minutes each. Rinsed slides for 3 minutes in running dH2O. Slides [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":28195,"comment_status":"closed","ping_status":"open","sticky":false,"template":"elementor_header_footer","format":"standard","meta":{"footnotes":""},"categories":[118,102],"tags":[],"class_list":["post-28196","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-he_4m","category-alcianblue"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v25.6 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Alcain Blue Staining of 4 Month-Old Human Lung from Donor D075-RLL-10B1 -<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/research.cchmc.org\/lungimage\/?p=28196\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Alcain Blue Staining of 4 Month-Old Human Lung from Donor D075-RLL-10B1 -\" \/>\n<meta property=\"og:description\" content=\"Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from\u00a0the University of Rochester Medical Center Paraffin sections were placed at 60oC overnight to melt paraffin. Paraffin sections were then placed in xylene (Xylenes Reagent Grade ACS Histology\/Lab Grade Cat # 399000000 Pharma-AAPER) 3X for 10 minutes each. Rinsed slides for 3 minutes in running dH2O. 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