{"id":28326,"date":"2018-10-15T18:56:09","date_gmt":"2018-10-15T18:56:09","guid":{"rendered":"https:\/\/research.cchmc.org\/lungimage\/?p=28326"},"modified":"2019-09-11T12:30:50","modified_gmt":"2019-09-11T12:30:50","slug":"alcian-blue-staining-of-14-month-old-human-lung-from-donor-d072-rll-13b4","status":"publish","type":"post","link":"https:\/\/research.cchmc.org\/lungimage\/?p=28326","title":{"rendered":"Alcian Blue Staining of 14 Month-Old Human Lung from Donor D072-LLL-13B4"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"28326\" class=\"elementor elementor-28326\" data-elementor-post-type=\"post\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-765d724b elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"765d724b\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container 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Staining of 14 Month-Old Human Lung from Donor D072-LLL-13B4\" data-description=\"4X Tile Scan\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">AB Staining of 14 Month-Old Human Lung from Donor D072-LLL-13B4<\/div><div class=\"html5gallery-description\">4X Tile Scan<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-15_4X_AB_LMH-18-D078-13B4.1_001.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-15_4X_AB_LMH-18-D078-13B4.1_001.jpg\" alt=\"AB Staining of 14 Month-Old Human Lung from Donor D072-LLL-13B4\" data-description=\"4X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">AB Staining of 14 Month-Old Human Lung from Donor D072-LLL-13B4<\/div><div class=\"html5gallery-description\">4X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-15_10X_AB_LMH-18-D078-13B4.1_001.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-15_10X_AB_LMH-18-D078-13B4.1_001.jpg\" alt=\"AB Staining of 14 Month-Old Human Lung from Donor D072-LLL-13B4\" data-description=\"10X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">AB Staining of 14 Month-Old Human Lung from Donor D072-LLL-13B4<\/div><div class=\"html5gallery-description\">10X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-15_20X_AB_LMH-18-D078-13B4.1_001.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-15_20X_AB_LMH-18-D078-13B4.1_001.jpg\" alt=\"AB Staining of 14 Month-Old Human Lung from Donor D072-LLL-13B4\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">AB Staining of 14 Month-Old Human Lung from Donor D072-LLL-13B4<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-15_20X_AB_LMH-18-D078-13B4.1_001-1.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-15_20X_AB_LMH-18-D078-13B4.1_001-1.jpg\" alt=\"AB Staining of 14 Month-Old Human Lung from Donor D072-LLL-13B4\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">AB Staining of 14 Month-Old Human Lung from Donor D072-LLL-13B4<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-15_10X_AB_LMH-18-D078-13B4.1_001a.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-15_10X_AB_LMH-18-D078-13B4.1_001a.jpg\" alt=\"AB Staining of 14 Month-Old Human Lung from Donor D072-LLL-13B4\" data-description=\"10X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">AB Staining of 14 Month-Old Human Lung from Donor D072-LLL-13B4<\/div><div class=\"html5gallery-description\">10X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-15_20X_AB_LMH-18-D078-13B4.1_001a.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-15_20X_AB_LMH-18-D078-13B4.1_001a.jpg\" alt=\"AB Staining of 14 Month-Old Human Lung from Donor D072-LLL-13B4\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">AB Staining of 14 Month-Old Human Lung from Donor D072-LLL-13B4<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-15_20X_AB_LMH-18-D078-13B4.1_001a-1.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-15_20X_AB_LMH-18-D078-13B4.1_001a-1.jpg\" alt=\"AB Staining of 14 Month-Old Human Lung from Donor D072-LLL-13B4\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">AB Staining of 14 Month-Old Human Lung from Donor D072-LLL-13B4<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-15_4X_AB_LMH-18-D078-13B4.1_002.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-15_4X_AB_LMH-18-D078-13B4.1_002.jpg\" alt=\"AB Staining of 14 Month-Old Human Lung from Donor D072-LLL-13B4\" data-description=\"4X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">AB Staining of 14 Month-Old Human Lung from Donor D072-LLL-13B4<\/div><div class=\"html5gallery-description\">4X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-15_10X_AB_LMH-18-D078-13B4.1_002.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-15_10X_AB_LMH-18-D078-13B4.1_002.jpg\" alt=\"AB Staining of 14 Month-Old Human Lung from Donor D072-LLL-13B4\" data-description=\"10X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">AB Staining of 14 Month-Old Human Lung from Donor D072-LLL-13B4<\/div><div class=\"html5gallery-description\">10X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-15_20X_AB_LMH-18-D078-13B4.1_002.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-15_20X_AB_LMH-18-D078-13B4.1_002.jpg\" alt=\"AB Staining of 14 Month-Old Human Lung from Donor D072-LLL-13B4\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">AB Staining of 14 Month-Old Human Lung from Donor D072-LLL-13B4<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-15_20X_AB_LMH-18-D078-13B4.1_002-1.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-15_20X_AB_LMH-18-D078-13B4.1_002-1.jpg\" alt=\"AB Staining of 14 Month-Old Human Lung from Donor D072-LLL-13B4\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">AB Staining of 14 Month-Old Human Lung from Donor D072-LLL-13B4<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-15_10X_AB_LMH-18-D078-13B4.1_002a.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-15_10X_AB_LMH-18-D078-13B4.1_002a.jpg\" alt=\"AB Staining of 14 Month-Old Human Lung from Donor D072-LLL-13B4\" data-description=\"10X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">AB Staining of 14 Month-Old Human Lung from Donor D072-LLL-13B4<\/div><div class=\"html5gallery-description\">10X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-15_20X_AB_LMH-18-D078-13B4.1_002a.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-15_20X_AB_LMH-18-D078-13B4.1_002a.jpg\" alt=\"AB Staining of 14 Month-Old Human Lung from Donor D072-LLL-13B4\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">AB Staining of 14 Month-Old Human Lung from Donor D072-LLL-13B4<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-15_20X_AB_LMH-18-D078-13B4.1_002a-1.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271365%27%20height%3D%271024%27%20viewBox%3D%270%200%201365%201024%27%3E%3Crect%20width%3D%271365%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/10\/2018-10-15_20X_AB_LMH-18-D078-13B4.1_002a-1.jpg\" alt=\"AB Staining of 14 Month-Old Human Lung from Donor D072-LLL-13B4\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">AB Staining of 14 Month-Old Human Lung from Donor D072-LLL-13B4<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><\/div><\/div><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-2e5b741f elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"2e5b741f\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-30bd8cd8\" data-id=\"30bd8cd8\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-256af15b elementor-widget elementor-widget-text-editor\" data-id=\"256af15b\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"margin: 0px 0px 20px; font-family: 'PT Sans', Arial, Helvetica, sans-serif;\">Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from\u00a0the University of Rochester Medical Center<\/p><p style=\"margin: 0px 0px 20px; font-family: 'PT Sans', Arial, Helvetica, sans-serif;\"><a href=\"https:\/\/www.urmc.rochester.edu\/labs\/Pryhuber-Lab\/\" target=\"_blank\" rel=\"noopener\"><img class=\"lazyload\" decoding=\"async\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27200%27%20height%3D%2748%27%20viewBox%3D%270%200%20200%2048%27%3E%3Crect%20width%3D%27200%27%20height%3D%2748%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2015\/07\/Pryhuber_Lab.jpg\" alt=\"Pryhuber_Lab\" width=\"200\" height=\"48\" \/><\/a><\/p><p style=\"margin: 0px 0px 20px; font-family: 'PT Sans', Arial, Helvetica, sans-serif;\">\u00a0<\/p><ol style=\"font-family: 'PT Sans', Arial, Helvetica, sans-serif;\"><li>For Frozen tissue, rinsed slides for 2X in PBS, then Incubated slides for 5 min in 4% PFA\/PBS, then rinsed slides 1X in PBS.<\/li><li>Rinsed slides for 3 minutes in running dH<sub>2<\/sub>O.<\/li><li>Slides were then incubated in 3% Glacial Acetic Acid (<a href=\"http:\/\/www.polyrnd.com\/acetic-acid-3-aqueous-10185.html\"><span style=\"font-weight: bold;\">S101<\/span><\/a>, Poly Scientific) for 3 minutes.<\/li><li>Slides were then incubated in filtered Alcian Blue (<a href=\"http:\/\/www.polyrnd.com\/alcian-blue-1-in-3-acetic-acid-ph-2-5-15114.html\"><span style=\"font-weight: bold;\">S111A<\/span><\/a>, Poly Scientific) for 30 minutes.<\/li><li>Wash slides under cold running tap water for 10 minutes.<\/li><li>Incubate for 2 minutes in Nuclear Fast Red (<a href=\"http:\/\/www.polyrnd.com\/nuclear-fast-red-kernechtrot-0-7.html\"><span style=\"font-weight: bold;\">S248<\/span><\/a>, Poly Scientific).<\/li><li>Wash under flowing\u00a0dH<sub>2<\/sub>O until excess Nuclear Fast Red is washed away.<\/li><li>Incubated\u00a0slides in 90% ETOH for 20 dips, repeat in different 90% ETOH.<\/li><li>Incubated slides in 100% ETOH for 20 dips, repeat in different\u00a0100% ETOH.<\/li><li>Washed\u00a0slides in 2X in Xylene, followed by 6 more washes in xylene.<\/li><li>Coverslip\u00a0with Permount. (Permount Toulene Solution UN 1294, Cat #\u00a0<a href=\"https:\/\/www.fishersci.com\/shop\/products\/fisher-chemical-permount-mounting-medium-2\/sp15500\"><span style=\"font-weight: bold;\">SP-15-500<\/span><\/a>\u00a0Fisher Scientific).<\/li><\/ol><p style=\"margin: 0px 0px 20px; font-family: 'PT Sans', Arial, Helvetica, sans-serif;\">\u00a0<\/p><p style=\"margin: 0px 0px 20px; font-family: 'PT Sans', Arial, Helvetica, sans-serif;\"><span style=\"font-weight: bold;\">\u00a0<\/span><\/p><p style=\"margin: 0px 0px 20px; font-family: 'PT Sans', Arial, Helvetica, sans-serif;\"><span style=\"font-weight: bold;\">Tissue Used:<br \/><\/span><span style=\"font-weight: bold; letter-spacing: 0px;\">LMH-18-D072-LLL-13B4.1<br \/><\/span><span style=\"font-weight: bold; letter-spacing: 0px;\">Gender: Female<br \/><\/span><span style=\"font-weight: bold; letter-spacing: 0px;\">Age: 14 Months<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-49e2925a elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"49e2925a\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-7d5bc1a7\" data-id=\"7d5bc1a7\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-c538b88 elementor-widget elementor-widget-facebook-comments\" data-id=\"c538b88\" data-element_type=\"widget\" data-widget_type=\"facebook-comments.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-facebook-widget fb-comments\" data-href=\"https:\/\/research.cchmc.org\/lungimage?p=28326\" data-width=\"100%\" data-numposts=\"10\" data-order-by=\"social\" style=\"min-height: 1px\"><\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-78d393a9\" data-id=\"78d393a9\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-67bc2d93 elementor-widget elementor-widget-sidebar\" data-id=\"67bc2d93\" data-element_type=\"widget\" data-widget_type=\"sidebar.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\n\t\t<div id=\"recent-posts-99\" class=\"widget widget_recent_entries\">\n\t\t<div class=\"heading\"><h4 class=\"widget-title\">Recent Experiments<\/h4><\/div>\n\t\t<ul>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=32066\">4 Year-Old Human Lung KRT14, MUC5B, and TUBA4A Confocal Imaging<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31223\">Alcian Blue Staining from PBR 3653 Patient with Cystic Fibrosis<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31202\">H&#038;E Staining from PBR 3383 Patient with Cystic Fibrosis<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31199\">H&#038;E Staining from PBR 3653 Patient with Cystic Fibrosis<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31196\">H&#038;E Staining of 31 Year-Old Human Donor 10<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31193\">H&#038;E Staining of 27 Year-Old Human Donor 3<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31190\">Alcian Blue Staining of 27 Year-Old Human Donor 3<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31186\">Alcian Blue Staining of 31 Year-Old Human Donor 10<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31182\">Alcian Blue Staining from PBR 3383 Patient with Cystic Fibrosis<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31024\">Alcian Blue Staining of 29 Year-Old Human Donor D175-10C1.21<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31019\">Alcian Blue Staining of 29 Year-Old Human Donor D175-11C4.14<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31016\">Alcian Blue Staining of 29 Year-Old Human Donor D175-11C2.11<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31013\">Alcian Blue Staining of 24 Year-Old Human Donor D071-23C2.19<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31010\">Alcian Blue Staining of 24 Year-Old Human Donor D071-17C2.20<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31007\">Alcian Blue Staining of 24 Year-Old Human Donor D071-16C3.27<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t<\/ul>\n\n\t\t<\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from\u00a0the University of Rochester Medical Center \u00a0 For Frozen tissue, rinsed slides for 2X in PBS, then Incubated slides for 5 min in 4% PFA\/PBS, then rinsed slides 1X in PBS. Rinsed slides for 3 minutes in running dH2O. Slides were then incubated in 3% Glacial Acetic [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":28321,"comment_status":"closed","ping_status":"open","sticky":false,"template":"elementor_header_footer","format":"standard","meta":{"footnotes":""},"categories":[302,102,103],"tags":[],"class_list":["post-28326","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-14-month-old-human-he","category-alcianblue","category-postnatal"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v25.6 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Alcian Blue Staining of 14 Month-Old Human Lung from Donor D072-LLL-13B4 -<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/research.cchmc.org\/lungimage\/?p=28326\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Alcian Blue Staining of 14 Month-Old Human Lung from Donor D072-LLL-13B4 -\" \/>\n<meta property=\"og:description\" content=\"Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from\u00a0the University of Rochester Medical Center \u00a0 For Frozen tissue, rinsed slides for 2X in PBS, then Incubated slides for 5 min in 4% PFA\/PBS, then rinsed slides 1X in PBS. Rinsed slides for 3 minutes in running dH2O. 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