{"id":28627,"date":"2018-10-24T11:54:48","date_gmt":"2018-10-24T11:54:48","guid":{"rendered":"https:\/\/research.cchmc.org\/lungimage\/?p=28627"},"modified":"2019-09-11T12:40:50","modified_gmt":"2019-09-11T12:40:50","slug":"he-staining-of-2-year-old-human-lung-from-donor-cchmc-d007-b29-3","status":"publish","type":"post","link":"https:\/\/research.cchmc.org\/lungimage\/?p=28627","title":{"rendered":"H&E Staining of 2 Year-Old Human Lung from Donor CCHMC-D007-B29.3"},"content":{"rendered":"\t\t
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H&E Staining of 2 Year-Old Human Lung from Donor CCHMC-D007-B29.3<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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\"H&E<\/a>
H&E Staining of 2 Year-Old Human Lung from Donor CCHMC-D007-B29.3<\/div>
4X Tile Scan<\/div><\/div>\"H&E<\/a>
H&E Staining of 2 Year-Old Human Lung from Donor CCHMC-D007-B29.3<\/div>
4X<\/div><\/div>\"H&E<\/a>
H&E Staining of 2 Year-Old Human Lung from Donor CCHMC-D007-B29.3<\/div>
10X<\/div><\/div>\"H&E<\/a>
H&E Staining of 2 Year-Old Human Lung from Donor CCHMC-D007-B29.3<\/div>
20X<\/div><\/div>\"H&E<\/a>
H&E Staining of 2 Year-Old Human Lung from Donor CCHMC-D007-B29.3<\/div>
20X<\/div><\/div>\"H&E<\/a>
H&E Staining of 2 Year-Old Human Lung from Donor CCHMC-D007-B29.3<\/div>
10X<\/div><\/div>\"H&E<\/a>
H&E Staining of 2 Year-Old Human Lung from Donor CCHMC-D007-B29.3<\/div>
20X<\/div><\/div>\"H&E<\/a>
H&E Staining of 2 Year-Old Human Lung from Donor CCHMC-D007-B29.3<\/div>
20X<\/div><\/div>\"H&E<\/a>
H&E Staining of 2 Year-Old Human Lung from Donor CCHMC-D007-B29.3<\/div>
4X<\/div><\/div>\"H&E<\/a>
H&E Staining of 2 Year-Old Human Lung from Donor CCHMC-D007-B29.3<\/div>
10X<\/div><\/div>\"H&E<\/a>
H&E Staining of 2 Year-Old Human Lung from Donor CCHMC-D007-B29.3<\/div>
20X<\/div><\/div>\"H&E<\/a>
H&E Staining of 2 Year-Old Human Lung from Donor CCHMC-D007-B29.3<\/div>
20X<\/div><\/div>\"H&E<\/a>
H&E Staining of 2 Year-Old Human Lung from Donor CCHMC-D007-B29.3<\/div>
10X<\/div><\/div>\"H&E<\/a>
H&E Staining of 2 Year-Old Human Lung from Donor CCHMC-D007-B29.3<\/div>
20X<\/div><\/div>\"H&E<\/a>
H&E Staining of 2 Year-Old Human Lung from Donor CCHMC-D007-B29.3<\/div>
20X<\/div><\/div>\"H&E<\/a>
H&E Staining of 2 Year-Old Human Lung from Donor CCHMC-D007-B29.3<\/div>
4X<\/div><\/div>\"H&E<\/a>
H&E Staining of 2 Year-Old Human Lung from Donor CCHMC-D007-B29.3<\/div>
10X<\/div><\/div>\"H&E<\/a>
H&E Staining of 2 Year-Old Human Lung from Donor CCHMC-D007-B29.3<\/div>
20X<\/div><\/div>\"H&E<\/a>
H&E Staining of 2 Year-Old Human Lung from Donor CCHMC-D007-B29.3<\/div>
20X<\/div><\/div>\"H&E<\/a>
H&E Staining of 2 Year-Old Human Lung from Donor CCHMC-D007-B29.3<\/div>
10X<\/div><\/div>\"H&E<\/a>
H&E Staining of 2 Year-Old Human Lung from Donor CCHMC-D007-B29.3<\/div>
20X<\/div><\/div>\"H&E<\/a>
H&E Staining of 2 Year-Old Human Lung from Donor CCHMC-D007-B29.3<\/div>
20X<\/div><\/div>\"H&E<\/a>
H&E Staining of 2 Year-Old Human Lung from Donor CCHMC-D007-B29.3<\/div>
4X<\/div><\/div>\"H&E<\/a>
H&E Staining of 2 Year-Old Human Lung from Donor CCHMC-D007-B29.3<\/div>
10X<\/div><\/div>\"H&E<\/a>
H&E Staining of 2 Year-Old Human Lung from Donor CCHMC-D007-B29.3<\/div>
20X<\/div><\/div>\"H&E<\/a>
H&E Staining of 2 Year-Old Human Lung from Donor CCHMC-D007-B29.3<\/div>
20X<\/div><\/div>\"H&E<\/a>
H&E Staining of 2 Year-Old Human Lung from Donor CCHMC-D007-B29.3<\/div>
10X<\/div><\/div>\"H&E<\/a>
H&E Staining of 2 Year-Old Human Lung from Donor CCHMC-D007-B29.3<\/div>
20X<\/div><\/div>\"H&E<\/a>
H&E Staining of 2 Year-Old Human Lung from Donor CCHMC-D007-B29.3<\/div>
20X<\/div><\/div><\/div><\/div><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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\u00a0<\/p>

\"\"<\/a><\/p>

The tissue studies were done in collaboration with Francis X. McCormack, MD and Kathryn A. Wikenheiser-Brokamp, MD\/PhD with support of\u00a0LifeCenter<\/a>\u00a0and\u00a0CCHMC Patholgy Research Core<\/a><\/strong><\/p>

  1. For Frozen tissue, rinsed slides for 2X in PBS, then Incubated slides for 5 min in 4% PFA\/PBS, then rinsed slides 1X in PBS.<\/li>
  2. Rinsed slides for 3 minutes in running dH2<\/sub>O.<\/li>
  3. Slides were then stained in filtered Hematoxylin for 60 seconds. (Gold Standard Harris Hemotoxylin, Acidified, Mercury; Cat # 24245 Polysciences Inc,).<\/li>
  4. Rinsed slides in tap water until water turned cleared then rinsed in dH2<\/sub>O<\/li>
  5. Rinsed slides in Lithium Carbonate: 20 dips. (Lithium Carbonate ACS Grade; Cat # 255823-500G Sigma Aldrich). Lithium carbonate is prepared by over-saturating in dH2<\/sub>O.<\/li>
  6. Rinsed slides in dH2<\/sub>O 3X 20 dips.<\/li>
  7. Rinsed slides in 70% ETOH for 20 dips.<\/li>
  8. Rinsed slides in 80% ETOH for 20 dips.<\/li>
  9. Stained slides in Eosin for 15 seconds. (Eosin Y 0.5% Alcohol Solution, Cat # 09859 Polysciences Inc,).<\/li>
  10. Rinsed slides in a series of 95% ETOH washes. 10 dips for the first and second washes, 15 dips in the third and fourth washes.<\/li>
  11. Rinsed slides 3X in 100% ETOH. 10 dips for the first and second washes and 15 dips in the third and fourth washes.<\/li>
  12. Rinsed slides in 2X in Xylene, followed by 6 more washes in xylene.<\/li>
  13. Coverslip with Permount. (Permount Toulene Solution UN 1294, Cat # SP-15-500 Fisher Scientific).<\/li><\/ol>

    Tissue Used:\u00a0
    <\/strong>LMH-18-CCHMC-D007-B29.3
    <\/b>Age: 2 Years
    Gender: Male<\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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    Recent Experiments<\/h4><\/div>\n\t\t