{"id":31223,"date":"2019-11-13T16:30:10","date_gmt":"2019-11-13T16:30:10","guid":{"rendered":"https:\/\/research.cchmc.org\/lungimage\/?p=31223"},"modified":"2019-11-13T16:34:11","modified_gmt":"2019-11-13T16:34:11","slug":"alcian-blue-staining-from-pbr-3653-patient-with-cystic-fibrosis","status":"publish","type":"post","link":"https:\/\/research.cchmc.org\/lungimage\/?p=31223","title":{"rendered":"Alcian Blue Staining from PBR 3653 Patient with Cystic Fibrosis"},"content":{"rendered":"\t\t
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Alcian Blue Staining from PBR 3653 Patient with Cystic Fibrosis<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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\"Alcian<\/a>
Alcian Blue Staining from PBR 3653 Patient with Cystic Fibrosis<\/div>
4X Tilescan<\/div><\/div>\"Alcian<\/a>
Alcian Blue Staining from PBR 3653 Patient with Cystic Fibrosis<\/div>
4X<\/div><\/div>\"Alcian<\/a>
Alcian Blue Staining from PBR 3653 Patient with Cystic Fibrosis<\/div>
10X<\/div><\/div>\"Alcian<\/a>
Alcian Blue Staining from PBR 3653 Patient with Cystic Fibrosis<\/div>
20X<\/div><\/div>\"Alcian<\/a>
Alcian Blue Staining from PBR 3653 Patient with Cystic Fibrosis<\/div>
4X<\/div><\/div>\"Alcian<\/a>
Alcian Blue Staining from PBR 3653 Patient with Cystic Fibrosis<\/div>
10X<\/div><\/div>\"Alcian<\/a>
Alcian Blue Staining from PBR 3653 Patient with Cystic Fibrosis<\/div>
20X<\/div><\/div>\"Alcian<\/a>
Alcian Blue Staining from PBR 3653 Patient with Cystic Fibrosis<\/div>
4X<\/div><\/div>\"Alcian<\/a>
Alcian Blue Staining from PBR 3653 Patient with Cystic Fibrosis<\/div>
10X<\/div><\/div>\"Alcian<\/a>
Alcian Blue Staining from PBR 3653 Patient with Cystic Fibrosis<\/div>
20X<\/div><\/div>\"Alcian<\/a>
Alcian Blue Staining from PBR 3653 Patient with Cystic Fibrosis<\/div>
4X<\/div><\/div>\"Alcian<\/a>
Alcian Blue Staining from PBR 3653 Patient with Cystic Fibrosis<\/div>
10X<\/div><\/div>\"Alcian<\/a>
Alcian Blue Staining from PBR 3653 Patient with Cystic Fibrosis<\/div>
20X<\/div><\/div>\"Alcian<\/a>
Alcian Blue Staining from PBR 3653 Patient with Cystic Fibrosis<\/div>
4X<\/div><\/div>\"Alcian<\/a>
Alcian Blue Staining from PBR 3653 Patient with Cystic Fibrosis<\/div>
10X<\/div><\/div>\"Alcian<\/a>
Alcian Blue Staining from PBR 3653 Patient with Cystic Fibrosis<\/div>
20X<\/div><\/div><\/div><\/div><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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\u00a0<\/p>

Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from\u00a0the University of Rochester Medical Center<\/p>

\"Pryhuber_Lab\"<\/a><\/p>

\u00a0<\/p>

  1. For Frozen tissue, rinsed slides for 2X in PBS, then Incubated slides for 5 min in 4% PFA\/PBS, then rinsed slides 1X in PBS.<\/li>
  2. Rinsed slides for 3 minutes in running dH2<\/sub>O.<\/li>
  3. Slides were then incubated in 3% Glacial Acetic Acid (S101<\/a>, Poly Scientific) for 3 minutes.<\/li>
  4. Slides were then incubated in filtered Alcian Blue (S111A<\/a>, Poly Scientific) for 30 minutes.<\/li>
  5. Wash slides under cold running tap water for 10 minutes.<\/li>
  6. Incubate for 2 minutes in Nuclear Fast Red (S248<\/a>, Poly Scientific).<\/li>
  7. Wash under flowing\u00a0dH2<\/sub>O until excess Nuclear Fast Red is washed away.<\/li>
  8. Incubated\u00a0slides in 90% ETOH for 20 dips, repeat in different 90% ETOH.<\/li>
  9. Incubated slides in 100% ETOH for 20 dips, repeat in different\u00a0100% ETOH.<\/li>
  10. Washed\u00a0slides in 2X in Xylene, followed by 6 more washes in xylene.<\/li>
  11. Coverslip\u00a0with Permount. (Permount Toulene Solution UN 1294, Cat #\u00a0SP-15-500<\/a>\u00a0Fisher Scientific).<\/li><\/ol>

    Tissue Used:<\/strong>
    Alcian Blue Staining from PBR 3653 Patient with Cystic Fibrosis<\/b><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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    <\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

    \u00a0 Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from\u00a0the University of Rochester Medical Center \u00a0 For Frozen tissue, rinsed slides for 2X in PBS, then Incubated slides for 5 min in 4% PFA\/PBS, then rinsed slides 1X in PBS. Rinsed slides for 3 minutes in running dH2O. Slides were then incubated in 3% Glacial […]<\/p>\n","protected":false},"author":1,"featured_media":31215,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-31223","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-uncategorized"],"yoast_head":"\nAlcian Blue Staining from PBR 3653 Patient with Cystic Fibrosis -<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31223\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Alcian Blue Staining from PBR 3653 Patient with Cystic Fibrosis -\" \/>\n<meta property=\"og:description\" content=\"\u00a0 Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from\u00a0the University of Rochester Medical Center \u00a0 For Frozen tissue, rinsed slides for 2X in PBS, then Incubated slides for 5 min in 4% PFA\/PBS, then rinsed slides 1X in PBS. Rinsed slides for 3 minutes in running dH2O. Slides were then incubated in 3% Glacial […]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/research.cchmc.org\/lungimage\/?p=31223\" \/>\n<meta property=\"article:published_time\" content=\"2019-11-13T16:30:10+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2019-11-13T16:34:11+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2019\/11\/2019-11-8_AB_10X_LMH-PBR3653_003a.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1365\" \/>\n\t<meta property=\"og:image:height\" content=\"1024\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"kitc5i\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"kitc5i\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"1 minute\" 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