{"id":3225,"date":"2015-05-09T01:48:23","date_gmt":"2015-05-09T01:48:23","guid":{"rendered":"https:\/\/research.cchmc.org\/lungimage\/?p=3225"},"modified":"2019-09-10T17:10:07","modified_gmt":"2019-09-10T17:10:07","slug":"he-staining-20-month-fetal-lung","status":"publish","type":"post","link":"https:\/\/research.cchmc.org\/lungimage\/?p=3225","title":{"rendered":"H&E Staining 20-Month Human Lung"},"content":{"rendered":"

\"H&E<\/a>
H&E Staining of 20 Month Female Human Lung<\/div>
4X Tile Scan<\/div><\/div>\"H&E<\/a>
H&E Staining of 20 Month Female Human Lung<\/div>
4X<\/div><\/div>\"H&E<\/a>
H&E Staining of 20 Month Female Human Lung<\/div>
10X<\/div><\/div>\"H&E<\/a>
H&E Staining of 20 Month Female Human Lung<\/div>
10X<\/div><\/div>\"H&E<\/a>
H&E Staining of 20 Month Female Human Lung<\/div>
20X<\/div><\/div>\"H&E<\/a>
H&E Staining of 20 Month Female Human Lung<\/div>
20X<\/div><\/div>\"H&E<\/a>
H&E Staining of 20 Month Female Human Lung<\/div>
4X<\/div><\/div>\"H&E<\/a>
H&E Staining of 20 Month Female Human Lung<\/div>
10X<\/div><\/div>\"H&E<\/a>
H&E Staining of 20 Month Female Human Lung<\/div>
10X<\/div><\/div>\"H&E<\/a>
H&E Staining of 20 Month Female Human Lung<\/div>
20X<\/div><\/div>\"H&E<\/a>
H&E Staining of 20 Month Female Human Lung<\/div>
4X<\/div><\/div>\"H&E<\/a>
H&E Staining of 20 Month Female Human Lung<\/div>
20X<\/div><\/div>\"H&E<\/a>
H&E Staining of 20 Month Female Human Lung<\/div>
20X<\/div><\/div>\"H&E<\/a>
H&E Staining of 20 Month Female Human Lung<\/div>
4X<\/div><\/div>\"H&E<\/a>
H&E Staining of 20 Month Female Human Lung<\/div>
10X<\/div><\/div>\"H&E<\/a>
H&E Staining of 20 Month Female Human Lung<\/div>
10X<\/div><\/div>\"H&E<\/a>
H&E Staining of 20 Month Female Human Lung<\/div>
20X<\/div><\/div>\"H&E<\/a>
H&E Staining of 20 Month Female Human Lung<\/div>
20X<\/div><\/div>\"H&E<\/a>
H&E Staining of 20 Month Female Human Lung<\/div>
4X<\/div><\/div>\"H&E<\/a>
H&E Staining of 20 Month Female Human Lung<\/div>
10X<\/div><\/div>\"H&E<\/a>
H&E Staining of 20 Month Female Human Lung<\/div>
10X<\/div><\/div>\"H&E<\/a>
H&E Staining of 20 Month Female Human Lung<\/div>
20X<\/div><\/div>\"H&E<\/a>
H&E Staining of 20 Month Female Human Lung<\/div>
20X<\/div><\/div>\"H&E<\/a>
H&E Staining of 20 Month Female Human Lung<\/div>
4X<\/div><\/div>\"H&E<\/a>
H&E Staining of 20 Month Female Human Lung<\/div>
10X<\/div><\/div>\"H&E<\/a>
H&E Staining of 20 Month Female Human Lung<\/div>
10X<\/div><\/div>\"H&E<\/a>
H&E Staining of 20 Month Female Human Lung<\/div>
20X<\/div><\/div>\"H&E<\/a>
H&E Staining of 20 Month Female Human Lung<\/div>
20X<\/div><\/div>\"H&E<\/a>
H&E Staining of 20 Month Female Human Lung<\/div>
40X<\/div><\/div><\/div><\/div>\n<\/div>
<\/div><\/div><\/div><\/div><\/div>

Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from\u00a0the University of Rochester Medical Center<\/p>\n

\"Pryhuber_Lab\"<\/a><\/p>\n

    \n
  1. Paraffin sections were placed at 60o<\/sup>C overnight to melt paraffin.<\/li>\n
  2. Paraffin sections were then placed in xylene (Xylenes Reagent Grade ACS Histology\/Lab Grade Cat # 399000000 Pharma-AAPER) 3X for 10 minutes each.<\/li>\n
  3. Rinsed slides in a series of 100% ETOH washes. 20 dips for the first wash, 3 minutes for the second wash, and 20 dips for the third wash.<\/li>\n
  4. Rinsed slides in a series of 95% ETOH washes. 20 dips for the first wash, 3 minutes for the second wash, and 20 dips for the third wash.<\/li>\n
  5. Rinsed slides in 70% ETOH for 3 min.<\/li>\n
  6. Rinsed slides for 3 minutes in running dH2<\/sub>O.<\/li>\n
  7. Slides were then stained in filtered Hematoxylin for 60 seconds. (Gold Standard Harris Hemotoxylin, Acidified, Mercury; Cat # 24245 Polysciences Inc,).<\/li>\n
  8. Rinsed slides in tap water until water turned cleared then rinsed in dH2<\/sub>O.<\/li>\n
  9. Rinsed slides in Lithium Carbonate: 20 dips. (Lithium Carbonate ACS Grade; Cat # 255823-500G Sigma Aldrich). Lithium carbonate is prepared by over-saturating in dH2<\/sub>O.<\/li>\n
  10. Rinsed slides in dH2<\/sub>O 3X 20 dips.<\/li>\n
  11. Rinsed slides in 70% ETOH for 20 dips.<\/li>\n
  12. Rinsed slides in 80% ETOH for 20 dips.<\/li>\n
  13. Stained slides in Eosin for 15 seconds. (Eosin Y 0.5% Alcohol Solution, Cat # 09859 Polysciences Inc,).<\/li>\n
  14. Rinsed slides in a series of 95% ETOH washes. 10 dips for the first and second washes, 15 dips in the third and fourth washes.<\/li>\n
  15. Rinsed slides 3X in 100% ETOH. 10 dips for the first and second washes and 15 dips in the third and fourth washes.<\/li>\n
  16. Rinsed slides in 2X in Xylene, followed by 6 more washes in xylene.<\/li>\n
  17. Coverslip with Permount. (Permount Toulene Solution UN 1294, Cat # SP-15-500 Fisher Scientific).<\/li>\n<\/ol>\n

    Tissue Used:<\/strong><\/p>\n

    LMH-D001-RLL-9C1-33<\/strong><\/p>\n

    Gender: <\/strong>Female<\/p>\n

    Age:<\/strong> 20 months<\/p>\n<\/div>

    <\/div><\/div><\/div><\/div><\/div><\/p>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":3228,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[117,72,77],"tags":[71],"class_list":["post-3225","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-he_20m","category-he","category-perinatalhuman-he","tag-he"],"yoast_head":"\nH&E Staining 20-Month Human Lung -<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/research.cchmc.org\/lungimage\/?p=3225\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"H&E Staining 20-Month Human Lung -\" \/>\n<meta property=\"og:url\" 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