{"id":6775,"date":"2016-04-08T19:20:53","date_gmt":"2016-04-08T19:20:53","guid":{"rendered":"https:\/\/research.cchmc.org\/lungimage\/?p=6775"},"modified":"2018-09-11T18:09:52","modified_gmt":"2018-09-11T18:09:52","slug":"e18-5-c57bl6-nkx2-1-abca3-and-acta2-confocal-imaging","status":"publish","type":"post","link":"https:\/\/research.cchmc.org\/lungimage\/?p=6775","title":{"rendered":"E18.5 C57BL6 NKX2.1, ABCA3, and ACTA2 Confocal Imaging"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"6775\" class=\"elementor elementor-6775 elementor-bc-flex-widget\" data-elementor-post-type=\"post\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-444cfa7b elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"444cfa7b\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-b9efaf7\" data-id=\"b9efaf7\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-2cc46536 elementor-widget elementor-widget-theme-post-title elementor-page-title elementor-widget-heading\" data-id=\"2cc46536\" data-element_type=\"widget\" data-widget_type=\"theme-post-title.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h1 class=\"elementor-heading-title elementor-size-large\">E18.5 C57BL6 NKX2.1, ABCA3, and ACTA2 Confocal Imaging<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-63505fff elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"63505fff\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-5f9c66b2\" data-id=\"5f9c66b2\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-a85f406 elementor-widget elementor-widget-text-editor\" data-id=\"a85f406\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><div class=\"wonderplugingallery-container\" id=\"wonderplugingallery-container-220\" style=\"max-width:550px;margin:0 auto;\"><div class=\"wonderplugingallery\" id=\"wonderplugingallery-220\" data-galleryid=\"220\" data-width=\"550\" data-height=\"550\" data-skin=\"light\" data-autoslide=\"false\" data-autoplayvideo=\"false\" data-html5player=\"true\" data-responsive=\"true\" data-fullwidth=\"false\" 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font:bold 12px Arial,Helvetica,sans-serif; overflow:hidden; white-space:normal; text-align:Center; line-height:18px;}\" data-lightboxdescriptioncss=\"{color:#333333; font:normal 12px Arial,Helvetica,sans-serif; overflow:hidden; white-space:normal; text-align:Center; line-height:14px;}\" data-titlecss=\"{color:#ffffff; font-size:14px; font-family:Armata, sans-serif, Arial; overflow:hidden; white-space:normal; text-align:Center; padding:10px 0px 10px 10px;  background:rgb(102, 102, 102) transparent; background:rgba(102, 102, 102, 0.6);}\" data-descriptioncss=\"{color:#ffffff; font-size:12px; font-family:Armata, sans-serif, Arial; overflow:hidden; white-space:normal; text-align:Center; padding:0px 0px 10px 10px;  background:rgb(102, 102, 102) transparent; background:rgba(102, 102, 102, 0.6);}\" data-jsfolder=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/plugins\/wonderplugin-gallery\/engine\/\" style=\"display:none;\" ><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/04\/2016-02-29_C57BL6_E18.5_LMM.14.27.6.65_NKX2.1_ABCA3_ACTA2-11.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271353%27%20height%3D%271024%27%20viewBox%3D%270%200%201353%201024%27%3E%3Crect%20width%3D%271353%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/04\/2016-02-29_C57BL6_E18.5_LMM.14.27.6.65_NKX2.1_ABCA3_ACTA2-11.jpg\" alt=\"E18.5 Confocal Imaging Showing Protein Expression of Nkx2.1, Abca3 and Acta2 Genes\" data-description=\"20x Tile Scan\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">E18.5 Confocal Imaging Showing Protein Expression of Nkx2.1, Abca3 and Acta2 Genes<\/div><div class=\"html5gallery-description\">20x Tile Scan<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/04\/2016-02-29_C57BL6_E18.5_LMM.14.27.6.65_NKX2.1_ABCA3_ACTA2-01.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/04\/2016-02-29_C57BL6_E18.5_LMM.14.27.6.65_NKX2.1_ABCA3_ACTA2-01.jpg\" alt=\"E18.5 Confocal Imaging Showing Protein Expression of Nkx2.1, Abca3 and Acta2 Genes\" data-description=\"20x\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">E18.5 Confocal Imaging Showing Protein Expression of Nkx2.1, Abca3 and Acta2 Genes<\/div><div class=\"html5gallery-description\">20x<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/04\/2016-02-29_C57BL6_E18.5_LMM.14.27.6.65_NKX2.1_ABCA3_ACTA2-02.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/04\/2016-02-29_C57BL6_E18.5_LMM.14.27.6.65_NKX2.1_ABCA3_ACTA2-02.jpg\" alt=\"E18.5 Confocal Imaging Showing Protein Expression of Nkx2.1, Abca3 and Acta2 Genes\" data-description=\"60x\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">E18.5 Confocal Imaging Showing Protein Expression of Nkx2.1, Abca3 and Acta2 Genes<\/div><div class=\"html5gallery-description\">60x<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/04\/2016-02-29_C57BL6_E18.5_LMM.14.27.6.65_NKX2.1_ABCA3_ACTA2-03.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/04\/2016-02-29_C57BL6_E18.5_LMM.14.27.6.65_NKX2.1_ABCA3_ACTA2-03.jpg\" alt=\"E18.5 Confocal Imaging Showing Protein Expression of Nkx2.1, Abca3 and Acta2 Genes\" data-description=\"60x\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">E18.5 Confocal Imaging Showing Protein Expression of Nkx2.1, Abca3 and Acta2 Genes<\/div><div class=\"html5gallery-description\">60x<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/04\/2016-02-29_C57BL6_E18.5_LMM.14.27.6.65_NKX2.1_ABCA3_ACTA2-04.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/04\/2016-02-29_C57BL6_E18.5_LMM.14.27.6.65_NKX2.1_ABCA3_ACTA2-04.jpg\" alt=\"E18.5 Confocal Imaging Showing Protein Expression of Nkx2.1, Abca3 and Acta2 Genes\" data-description=\"20x\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">E18.5 Confocal Imaging Showing Protein Expression of Nkx2.1, Abca3 and Acta2 Genes<\/div><div class=\"html5gallery-description\">20x<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/04\/2016-02-29_C57BL6_E18.5_LMM.14.27.6.65_NKX2.1_ABCA3_ACTA2-05.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/04\/2016-02-29_C57BL6_E18.5_LMM.14.27.6.65_NKX2.1_ABCA3_ACTA2-05.jpg\" alt=\"E18.5 Confocal Imaging Showing Protein Expression of Nkx2.1, Abca3 and Acta2 Genes\" data-description=\"60x\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">E18.5 Confocal Imaging Showing Protein Expression of Nkx2.1, Abca3 and Acta2 Genes<\/div><div class=\"html5gallery-description\">60x<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/04\/2016-02-29_C57BL6_E18.5_LMM.14.27.6.65_NKX2.1_ABCA3_ACTA2-06.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/04\/2016-02-29_C57BL6_E18.5_LMM.14.27.6.65_NKX2.1_ABCA3_ACTA2-06.jpg\" alt=\"E18.5 Confocal Imaging Showing Protein Expression of Nkx2.1, Abca3 and Acta2 Genes\" data-description=\"20x\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">E18.5 Confocal Imaging Showing Protein Expression of Nkx2.1, Abca3 and Acta2 Genes<\/div><div class=\"html5gallery-description\">20x<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/04\/2016-02-29_C57BL6_E18.5_LMM.14.27.6.65_NKX2.1_ABCA3_ACTA2-07.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/04\/2016-02-29_C57BL6_E18.5_LMM.14.27.6.65_NKX2.1_ABCA3_ACTA2-07.jpg\" alt=\"E18.5 Confocal Imaging Showing Protein Expression of Nkx2.1, Abca3 and Acta2 Genes\" data-description=\"60x\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">E18.5 Confocal Imaging Showing Protein Expression of Nkx2.1, Abca3 and Acta2 Genes<\/div><div class=\"html5gallery-description\">60x<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/04\/2016-02-29_C57BL6_E18.5_LMM.14.27.6.65_NKX2.1_ABCA3_ACTA2-08.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/04\/2016-02-29_C57BL6_E18.5_LMM.14.27.6.65_NKX2.1_ABCA3_ACTA2-08.jpg\" alt=\"E18.5 Confocal Imaging Showing Protein Expression of Nkx2.1, Abca3 and Acta2 Genes\" data-description=\"20x\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">E18.5 Confocal Imaging Showing Protein Expression of Nkx2.1, Abca3 and Acta2 Genes<\/div><div class=\"html5gallery-description\">20x<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/04\/2016-02-29_C57BL6_E18.5_LMM.14.27.6.65_NKX2.1_ABCA3_ACTA2-09.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/04\/2016-02-29_C57BL6_E18.5_LMM.14.27.6.65_NKX2.1_ABCA3_ACTA2-09.jpg\" alt=\"E18.5 Confocal Imaging Showing Protein Expression of Nkx2.1, Abca3 and Acta2 Genes\" data-description=\"60x\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">E18.5 Confocal Imaging Showing Protein Expression of Nkx2.1, Abca3 and Acta2 Genes<\/div><div class=\"html5gallery-description\">60x<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/04\/2016-02-29_C57BL6_E18.5_LMM.14.27.6.65_NKX2.1_ABCA3_ACTA2-10.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/04\/2016-02-29_C57BL6_E18.5_LMM.14.27.6.65_NKX2.1_ABCA3_ACTA2-10.jpg\" alt=\"E18.5 Confocal Imaging Showing Protein Expression of Nkx2.1, Abca3 and Acta2 Genes\" data-description=\"60x\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">E18.5 Confocal Imaging Showing Protein Expression of Nkx2.1, Abca3 and Acta2 Genes<\/div><div class=\"html5gallery-description\">60x<\/div><\/div><\/div><\/div><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-25c3be46 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"25c3be46\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-3baa4741\" data-id=\"3baa4741\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-32855489 elementor-widget elementor-widget-template\" data-id=\"32855489\" data-element_type=\"widget\" data-widget_type=\"template.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-template\">\n\t\t\t\t\t<div data-elementor-type=\"page\" data-elementor-id=\"24586\" class=\"elementor elementor-24586 elementor-bc-flex-widget\" data-elementor-post-type=\"elementor_library\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-4650a05 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"4650a05\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-951322a\" data-id=\"951322a\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-4f951ba\" data-id=\"4f951ba\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-0022067\" data-id=\"0022067\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-113c3c56 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"113c3c56\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-704be2d9\" data-id=\"704be2d9\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-70ecbadc elementor-widget elementor-widget-text-editor\" data-id=\"70ecbadc\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>Day 1<\/strong><\/p><ol><li>For Frozen tissue, rinse 2X in PBS, then 5 min in 4% PFA\/PBS, then rinse 1X in PBS.<\/li><li>Briefly equilibrate slides in Antigen Retrieval Buffer.<\/li><li>Antigen retrieval, pH 6.0 (times will vary according to microwave).<\/li><li>10 mM sodium citrate, pH 6.0, and heat in a microwave at 96<sup>o<\/sup>C.\u00a0*We usually do a series of three runs (6-7 minutes each run) to equal the time\/temp because of evaporation (refill coplin jars with dH<sub>2<\/sub>O).<\/li><li>Microwave according to instructions on microwave.<\/li><li>Cool on countertop, 15 min.<\/li><li>Rinse with dH<sub>2<\/sub>O<\/li><li>1X PBS, 5 min.<\/li><li>Block in 4% Goat serum\/PBS-T, 2 hours at RT.<\/li><li>For rabbit anti-<strong>NKX2.1<\/strong> (RB TTF-1 1231, Lot# 0912A) dilute 1:1000, for guinea pig anti-<strong>ABCA3<\/strong> (G985, BL 3\/10\/05, Seven Hills) dilute 1:100, for mouse anti-<a href=\"http:\/\/www.sigmaaldrich.com\/catalog\/product\/sigma\/a5228?lang=en%C2%AEion=US\" target=\"\"><strong>ACTA2<\/strong><\/a> (<a href=\"http:\/\/www.sigmaaldrich.com\/catalog\/product\/sigma\/a5228?lang=en%C2%AEion=US\" target=\"\"><strong>\u03b1 Smooth muscle actin<\/strong><\/a>, A5228, Sigma)\u00a0dilute 1:2000 in blocking buffer. Spin down in \u00b5fuge for 10 minutes and incubate on tissue overnight @ 4<sup>o<\/sup>C.<\/li><\/ol><p><strong>Day 2 <\/strong><\/p><ol><li>Rinse slides in PBS-T 3X, 5 min.<\/li><li>Apply secondary antibody, Goat Alexa Fluor 488 anti-rabbit IgG (<a href=\"https:\/\/www.thermofisher.com\/order\/genome-database\/antibody\/Goat-anti-Rabbit-IgG-H-L-Secondary-Antibody-Polyclonal\/A-11034\" target=\"\"><strong>A11034<\/strong><\/a>, Lot# 1298480, for anti-<strong> NKX2.1<\/strong>) at 1:200, Goat Alexa Fluor 555 anti-guinea pig IgG (<a href=\"https:\/\/www.thermofisher.com\/order\/genome-database\/antibody\/Goat-anti-Guinea-Pig-IgG-H-L-Secondary-Antibody-Polyclonal\/A-21435\" target=\"\"><strong>A21435<\/strong><\/a>, Lot# 1571699, for anti-<strong>ABCA3<\/strong>) at 1:200, Donkey Alexa Fluor 647 anti-mouse IgG <a href=\"https:\/\/www.thermofisher.com\/order\/genome-database\/antibody\/Donkey-anti-Mouse-IgG-H-L-Secondary-Antibody-Polyclonal\/A-31571\" target=\"\"><strong>A31571<\/strong><\/a>, Lot# 1549801, anti-<a href=\"http:\/\/www.sigmaaldrich.com\/catalog\/product\/sigma\/a5228?lang=en%C2%AEion=US\" target=\"\"><strong>ACTA2<\/strong><\/a>) at 1:200, in blocking buffer. Spin down in \u00b5fuge for 10 min, apply to tissue and incubated at room temperature for 1 hour.<\/li><\/ol><ol start=\"3\"><li>Rinse in PBS-T 3X, 5 min.<\/li><li>Dilute DAPI 1:2000 and apply to slides for 15 min.<\/li><li>Wash in PBS-T 3X, 5 min.<\/li><li>Rinse slides in 0.1M PB, 3X, 5 min.<\/li><li>Add 1 drop of Prolong Gold anti-fade mounting medium (P36930).<\/li><li>Coverslip with Gold Seal Coverslip (Cat# 3422 Electron Microscopy Sciences, 22 X 22 mm).<\/li><li>Allow Prolong Gold to cure overnight at room temp in light sealed box.<\/li><li>Store slides in light sealed box @ 4<sup>o<\/sup><\/li><\/ol><p><strong>Tissue Used: \u00a0\u00a0 <\/strong><strong>LMM.14.27.6.65<\/strong><\/p><p><strong>E18.5 C57BL6<\/strong><\/p><p><strong>Crown-to-rump: 21mm<\/strong><\/p><p><strong>Gender: Male<\/strong><\/p><p><strong>Weight: 1.32g<\/strong><\/p><p><span style=\"font-weight: bold;\">Appendix<\/span><\/p><p><span style=\"font-weight: bold;\">Antigen Retrieval Solution<\/span><\/p><p>18ml Solution A- 0.1M Citric Acid (Sigma, C1909) pH 2.5<\/p><p>82ml Reagent B- 0.1M Sodium Citrate (Sigma, S4641 ) pH 8.2<\/p><p>1L dH<sub>2<\/sub>O<\/p><p>pH 6.0<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-3fe951a7 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"3fe951a7\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-495efdd0\" data-id=\"495efdd0\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-378f7ca8 elementor-widget 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data-widget_type=\"sidebar.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div id=\"search-38\" class=\"widget widget_search\">\t\t<form role=\"search\" class=\"searchform fusion-search-form  fusion-search-form-classic\" method=\"get\" action=\"https:\/\/research.cchmc.org\/lungimage\/\">\n\t\t\t<div class=\"fusion-search-form-content\">\n\n\t\t\t\t\n\t\t\t\t<div class=\"fusion-search-field search-field\">\n\t\t\t\t\t<label><span class=\"screen-reader-text\">Search for:<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<input type=\"search\" value=\"\" name=\"s\" class=\"s\" placeholder=\"Search...\" required aria-required=\"true\" aria-label=\"Search...\"\/>\n\t\t\t\t\t\t\t\t\t\t\t<\/label>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"fusion-search-button search-button\">\n\t\t\t\t\t<input type=\"submit\" class=\"fusion-search-submit searchsubmit\" aria-label=\"Search\" value=\"&#xf002;\" 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style=\"overflow:hidden;\"><ul class=\"amazingcarousel-list\"><li class=\"amazingcarousel-item\"><div class=\"amazingcarousel-item-container\"><div class=\"amazingcarousel-image\">\n\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=6718\" target=\"target=\"_Blank\"\"><img decoding=\"async\" class=\"lazyload amazingcarousel-image-img\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27300%27%20height%3D%27300%27%20viewBox%3D%270%200%20300%20300%27%3E%3Crect%20width%3D%27300%27%20height%3D%27300%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/04\/2016-02-24_C57BL6_E16.5_LMM.14.24.4.33_NKX2.1_ABCA3_ACTA2-03-1.jpg\" alt=\"E16.5 C57BL6 NKX2.1, ABCA3, and ACTA2 Confocal Imaging\" data-description=\"\" \/><\/a>\n\t<div class=\"amazingcarousel-text\">\n\t\t<div class=\"amazingcarousel-text-bg\"><\/div>\n\t\t<div class=\"amazingcarousel-title\">E16.5 C57BL6 NKX2.1, ABCA3, and ACTA2 Confocal Imaging<\/div>\n\t<\/div>\n<\/div><\/div><\/li><li class=\"amazingcarousel-item\"><div class=\"amazingcarousel-item-container\"><div class=\"amazingcarousel-image\">\n\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=6775\" target=\"target=\"_Blank\"\"><img decoding=\"async\" class=\"lazyload amazingcarousel-image-img\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27300%27%20height%3D%27300%27%20viewBox%3D%270%200%20300%20300%27%3E%3Crect%20width%3D%27300%27%20height%3D%27300%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/04\/2016-02-29_C57BL6_E18.5_LMM.14.27.6.65_NKX2.1_ABCA3_ACTA2-03-1.jpg\" alt=\"E18.5 C57BL6 NKX2.1, ABCA3, and ACTA2 Confocal Imaging\" data-description=\"\" \/><\/a>\n\t<div class=\"amazingcarousel-text\">\n\t\t<div class=\"amazingcarousel-text-bg\"><\/div>\n\t\t<div class=\"amazingcarousel-title\">E18.5 C57BL6 NKX2.1, ABCA3, and ACTA2 Confocal Imaging<\/div>\n\t<\/div>\n<\/div><\/div><\/li><li class=\"amazingcarousel-item\"><div class=\"amazingcarousel-item-container\"><div class=\"amazingcarousel-image\">\n\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=6884\" target=\"target=\"_Blank\"\"><img decoding=\"async\" class=\"lazyload amazingcarousel-image-img\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27300%27%20height%3D%27300%27%20viewBox%3D%270%200%20300%20300%27%3E%3Crect%20width%3D%27300%27%20height%3D%27300%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/04\/2016-02-15_C57BL6_PND07_LMM.15.23A.4L.54_NKX2.1_ABCA3_ACTA2-03-1.jpg\" alt=\"Postnatal Day 7 C57BL6 NKX2.1, ABCA3, and ACTA2 Confocal Imaging\" data-description=\"\" \/><\/a>\n\t<div class=\"amazingcarousel-text\">\n\t\t<div class=\"amazingcarousel-text-bg\"><\/div>\n\t\t<div class=\"amazingcarousel-title\">Postnatal Day 7 C57BL6 NKX2.1, ABCA3, and ACTA2 Confocal Imaging<\/div>\n\t<\/div>\n<\/div><\/div><\/li><li class=\"amazingcarousel-item\"><div class=\"amazingcarousel-item-container\"><div class=\"amazingcarousel-image\">\n\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=6588\" target=\"target=\"_Blank\"\"><img decoding=\"async\" class=\"lazyload amazingcarousel-image-img\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27300%27%20height%3D%27300%27%20viewBox%3D%270%200%20300%20300%27%3E%3Crect%20width%3D%27300%27%20height%3D%27300%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/04\/2016-02-11_C57BL6_PND14_LMM.15.33A.6L.2_NKX2.1_ABCA3_ACTA2-07-1.jpg\" alt=\"Postnatal Day 14 C57BL6 NKX2.1, ABCA3, and ACTA2 Confocal Imaging\" data-description=\"\" \/><\/a>\n\t<div class=\"amazingcarousel-text\">\n\t\t<div class=\"amazingcarousel-text-bg\"><\/div>\n\t\t<div class=\"amazingcarousel-title\">Postnatal Day 14 C57BL6 NKX2.1, ABCA3, and ACTA2 Confocal Imaging<\/div>\n\t<\/div>\n<\/div><\/div><\/li><li class=\"amazingcarousel-item\"><div class=\"amazingcarousel-item-container\"><div class=\"amazingcarousel-image\">\n\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=7014\" target=\"target=\"_Blank\"\"><img decoding=\"async\" class=\"lazyload amazingcarousel-image-img\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27300%27%20height%3D%27300%27%20viewBox%3D%270%200%20300%20300%27%3E%3Crect%20width%3D%27300%27%20height%3D%27300%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/04\/2016-02-11_C57BL6_PND28_LMM.15.26A.1L.46_NKX2.1_ABCA3_ACTA2-10-2.jpg\" alt=\"Postnatal Day 28 C57BL6 NKX2.1, ABCA3, and ACTA2 Confocal Imaging\" data-description=\"\" \/><\/a>\n\t<div class=\"amazingcarousel-text\">\n\t\t<div class=\"amazingcarousel-text-bg\"><\/div>\n\t\t<div class=\"amazingcarousel-title\">Postnatal Day 28 C57BL6 NKX2.1, ABCA3, and ACTA2 Confocal Imaging<\/div>\n\t<\/div>\n<\/div><\/div><\/li><\/ul><div class=\"amazingcarousel-prev\"><\/div><div class=\"amazingcarousel-next\"><\/div><\/div><div class=\"amazingcarousel-nav\"><\/div><\/div><\/div><script>function wondercarousel_138_appendcss(csscode) {var head=document.head || document.getElementsByTagName(\"head\")[0];var style=document.createElement(\"style\");head.appendChild(style);style.type=\"text\/css\";if (style.styleSheet){style.styleSheet.cssText=csscode;} else {style.appendChild(document.createTextNode(csscode));}};wondercarousel_138_appendcss(\"@import url(https:\/\/fonts.googleapis.com\/css?family=Open+Sans);  #wonderplugincarousel-138 .amazingcarousel-image {\t \tposition: relative; \tpadding: 0px; }  #wonderplugincarousel-138 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}.amazingcarousel-socialmedia-button a { box-shadow: none; }.amazingcarousel-socialmedia-icon { display:table-cell; width:32px; height:32px; font-size:18px; border-radius:50%; color:#fff; vertical-align:middle; text-align:center; cursor:pointer; padding:0;}.amazingcarousel-socialmedia-rotate { transition: transform .4s ease-in; } .amazingcarousel-socialmedia-rotate:hover { transform: rotate(360deg); }\");<\/script><\/div>\n\t\t<\/div>\n\t\t<div id=\"recent-posts-36\" class=\"widget widget_recent_entries\">\n\t\t<div class=\"heading\"><h4 class=\"widget-title\">Recent Experiments<\/h4><\/div>\n\t\t<ul>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=32066\">4 Year-Old Human Lung KRT14, MUC5B, and TUBA4A Confocal Imaging<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31223\">Alcian Blue Staining from PBR 3653 Patient with Cystic Fibrosis<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31202\">H&#038;E Staining from PBR 3383 Patient with Cystic Fibrosis<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31199\">H&#038;E Staining from PBR 3653 Patient with Cystic Fibrosis<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31196\">H&#038;E Staining of 31 Year-Old Human Donor 10<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31193\">H&#038;E Staining of 27 Year-Old Human Donor 3<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31190\">Alcian Blue Staining of 27 Year-Old Human Donor 3<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31186\">Alcian Blue Staining of 31 Year-Old Human Donor 10<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31182\">Alcian Blue Staining from PBR 3383 Patient with Cystic Fibrosis<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31024\">Alcian Blue Staining of 29 Year-Old Human Donor D175-10C1.21<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31019\">Alcian Blue Staining of 29 Year-Old Human Donor D175-11C4.14<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31016\">Alcian Blue Staining of 29 Year-Old Human Donor D175-11C2.11<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31013\">Alcian Blue Staining of 24 Year-Old Human Donor D071-23C2.19<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31010\">Alcian Blue Staining of 24 Year-Old Human Donor D071-17C2.20<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31007\">Alcian Blue Staining of 24 Year-Old Human Donor D071-16C3.27<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t<\/ul>\n\n\t\t<\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Day 1 For Frozen tissue, rinse 2X in PBS, then 5 min in 4% PFA\/PBS, then rinse 1X in PBS. Briefly equilibrate slides in Antigen Retrieval Buffer. Antigen retrieval, pH 6.0 (times will vary according to microwave). 10 mM sodium citrate, pH 6.0, and heat in a microwave at 96oC.\u00a0*We usually do a series of [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":6777,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[83,274],"tags":[68,5,6,22,121,36],"class_list":["post-6775","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-e18-5","category-c57bl6","tag-abca3","tag-acta2","tag-alpha-smooth-muscle-actin","tag-nkx2-1","tag-sma","tag-ttf-1"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v25.6 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>E18.5 C57BL6 NKX2.1, ABCA3, and ACTA2 Confocal Imaging -<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/research.cchmc.org\/lungimage\/?p=6775\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"E18.5 C57BL6 NKX2.1, ABCA3, and ACTA2 Confocal Imaging -\" \/>\n<meta property=\"og:description\" content=\"Day 1 For Frozen tissue, rinse 2X in PBS, then 5 min in 4% PFA\/PBS, then rinse 1X in PBS. 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