{"id":8347,"date":"2016-05-20T14:26:57","date_gmt":"2016-05-20T14:26:57","guid":{"rendered":"https:\/\/research.cchmc.org\/lungimage\/?p=8347"},"modified":"2018-09-27T13:26:37","modified_gmt":"2018-09-27T13:26:37","slug":"4-year-old-human-lung-hopx-ager-acta2-confocal-imaging","status":"publish","type":"post","link":"https:\/\/research.cchmc.org\/lungimage\/?p=8347","title":{"rendered":"4 Year-Old Human Lung HOPX, AGER, ACTA2 Confocal Imaging"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"8347\" class=\"elementor elementor-8347 elementor-bc-flex-widget\" data-elementor-post-type=\"post\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-5dac4a8d elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"5dac4a8d\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-634c7257\" data-id=\"634c7257\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-1ea84992 elementor-widget elementor-widget-theme-post-title elementor-page-title elementor-widget-heading\" data-id=\"1ea84992\" data-element_type=\"widget\" data-widget_type=\"theme-post-title.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h1 class=\"elementor-heading-title elementor-size-large\">4 Year-Old Human Lung HOPX, AGER, ACTA2 Confocal Imaging<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-5110b5e0 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"5110b5e0\" data-element_type=\"section\">\n\t\t\t\t\t\t<div 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class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/05\/2016-05-18_LMH-15-D007-LLL-5C4.4_HOPX_AGER_ACTA2-01.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/05\/2016-05-18_LMH-15-D007-LLL-5C4.4_HOPX_AGER_ACTA2-01.jpg\" alt=\"4 Year Human Lung Confocal Imaging Showing Protein Expression of HOPX, AGER, and Acta2 Genes\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">4 Year Human Lung Confocal Imaging Showing Protein Expression of HOPX, AGER, and Acta2 Genes<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/05\/2016-05-18_LMH-15-D007-LLL-5C4.4_HOPX_AGER_ACTA2-02.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/05\/2016-05-18_LMH-15-D007-LLL-5C4.4_HOPX_AGER_ACTA2-02.jpg\" alt=\"4 Year Human Lung Confocal Imaging Showing Protein Expression of HOPX, AGER, and Acta2 Genes\" data-description=\"60X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">4 Year Human Lung Confocal Imaging Showing Protein Expression of HOPX, AGER, and Acta2 Genes<\/div><div class=\"html5gallery-description\">60X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/05\/2016-05-18_LMH-15-D007-LLL-5C4.4_HOPX_AGER_ACTA2-03.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/05\/2016-05-18_LMH-15-D007-LLL-5C4.4_HOPX_AGER_ACTA2-03.jpg\" alt=\"4 Year Human Lung Confocal Imaging Showing Protein Expression of HOPX, AGER, and Acta2 Genes\" data-description=\"60X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">4 Year Human Lung Confocal Imaging Showing Protein Expression of HOPX, AGER, and Acta2 Genes<\/div><div class=\"html5gallery-description\">60X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/05\/2016-05-18_LMH-15-D007-LLL-5C4.4_HOPX_AGER_ACTA2-04.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/05\/2016-05-18_LMH-15-D007-LLL-5C4.4_HOPX_AGER_ACTA2-04.jpg\" alt=\"4 Year Human Lung Confocal Imaging Showing Protein Expression of HOPX, AGER, and Acta2 Genes\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">4 Year Human Lung Confocal Imaging Showing Protein Expression of HOPX, AGER, and Acta2 Genes<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/05\/2016-05-18_LMH-15-D007-LLL-5C4.4_HOPX_AGER_ACTA2-05.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/05\/2016-05-18_LMH-15-D007-LLL-5C4.4_HOPX_AGER_ACTA2-05.jpg\" alt=\"4 Year Human Lung Confocal Imaging Showing Protein Expression of HOPX, AGER, and Acta2 Genes\" data-description=\"60X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">4 Year Human Lung Confocal Imaging Showing Protein Expression of HOPX, AGER, and Acta2 Genes<\/div><div class=\"html5gallery-description\">60X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/05\/2016-05-18_LMH-15-D007-LLL-5C4.4_HOPX_AGER_ACTA2-06.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/05\/2016-05-18_LMH-15-D007-LLL-5C4.4_HOPX_AGER_ACTA2-06.jpg\" alt=\"4 Year Human Lung Confocal Imaging Showing Protein Expression of HOPX, AGER, and Acta2 Genes\" data-description=\"60X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">4 Year Human Lung Confocal Imaging Showing Protein Expression of HOPX, AGER, and Acta2 Genes<\/div><div class=\"html5gallery-description\">60X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/05\/2016-05-18_LMH-15-D007-LLL-5C4.4_HOPX_AGER_ACTA2-07.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/05\/2016-05-18_LMH-15-D007-LLL-5C4.4_HOPX_AGER_ACTA2-07.jpg\" alt=\"4 Year Human Lung Confocal Imaging Showing Protein Expression of HOPX, AGER, and Acta2 Genes\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">4 Year Human Lung Confocal Imaging Showing Protein Expression of HOPX, AGER, and Acta2 Genes<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/05\/2016-05-18_LMH-15-D007-LLL-5C4.4_HOPX_AGER_ACTA2-08.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/05\/2016-05-18_LMH-15-D007-LLL-5C4.4_HOPX_AGER_ACTA2-08.jpg\" alt=\"4 Year Human Lung Confocal Imaging Showing Protein Expression of HOPX, AGER, and Acta2 Genes\" data-description=\"60X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">4 Year Human Lung Confocal Imaging Showing Protein Expression of HOPX, AGER, and Acta2 Genes<\/div><div class=\"html5gallery-description\">60X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/05\/2016-05-18_LMH-15-D007-LLL-5C4.4_HOPX_AGER_ACTA2-09.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/05\/2016-05-18_LMH-15-D007-LLL-5C4.4_HOPX_AGER_ACTA2-09.jpg\" alt=\"4 Year Human Lung Confocal Imaging Showing Protein Expression of HOPX, AGER, and Acta2 Genes\" data-description=\"60X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">4 Year Human Lung Confocal Imaging Showing Protein Expression of HOPX, AGER, and Acta2 Genes<\/div><div class=\"html5gallery-description\">60X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/05\/2016-05-18_LMH-15-D007-LLL-5C4.4_HOPX_AGER_ACTA2-10.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/05\/2016-05-18_LMH-15-D007-LLL-5C4.4_HOPX_AGER_ACTA2-10.jpg\" alt=\"4 Year Human Lung Confocal Imaging Showing Protein Expression of HOPX, AGER, and Acta2 Genes\" data-description=\"20X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">4 Year Human Lung Confocal Imaging Showing Protein Expression of HOPX, AGER, and Acta2 Genes<\/div><div class=\"html5gallery-description\">20X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/05\/2016-05-18_LMH-15-D007-LLL-5C4.4_HOPX_AGER_ACTA2-11.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/05\/2016-05-18_LMH-15-D007-LLL-5C4.4_HOPX_AGER_ACTA2-11.jpg\" alt=\"4 Year Human Lung Confocal Imaging Showing Protein Expression of HOPX, AGER, and Acta2 Genes\" data-description=\"60X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">4 Year Human Lung Confocal Imaging Showing Protein Expression of HOPX, AGER, and Acta2 Genes<\/div><div class=\"html5gallery-description\">60X<\/div><\/div><a class=\"html5galleryimglink\" href=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/05\/2016-05-18_LMH-15-D007-LLL-5C4.4_HOPX_AGER_ACTA2-12.jpg\" data-mediatype=1><img decoding=\"async\" class=\"lazyload html5galleryimg html5gallery-tn-image\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%271024%27%20height%3D%271024%27%20viewBox%3D%270%200%201024%201024%27%3E%3Crect%20width%3D%271024%27%20height%3D%271024%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/05\/2016-05-18_LMH-15-D007-LLL-5C4.4_HOPX_AGER_ACTA2-12.jpg\" alt=\"4 Year Human Lung Confocal Imaging Showing Protein Expression of HOPX, AGER, and Acta2 Genes\" data-description=\"60X\"><\/a><div class=\"html5gallery-info\"><div class=\"html5gallery-title\">4 Year Human Lung Confocal Imaging Showing Protein Expression of HOPX, AGER, and Acta2 Genes<\/div><div class=\"html5gallery-description\">60X<\/div><\/div><\/div><\/div><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-746dd653 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"746dd653\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-200dba5e\" data-id=\"200dba5e\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-6499ed25 elementor-widget elementor-widget-template\" data-id=\"6499ed25\" data-element_type=\"widget\" data-widget_type=\"template.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-template\">\n\t\t\t\t\t<div data-elementor-type=\"section\" data-elementor-id=\"26498\" class=\"elementor elementor-26498 elementor-bc-flex-widget\" data-elementor-post-type=\"elementor_library\">\n\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-4650a05 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"4650a05\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-951322a\" data-id=\"951322a\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-4f951ba\" data-id=\"4f951ba\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-0022067\" data-id=\"0022067\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-1a4f192d elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"1a4f192d\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-262f2d7b\" data-id=\"262f2d7b\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-3d25e9e9 elementor-widget elementor-widget-text-editor\" data-id=\"3d25e9e9\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>\u00a0\u00a0Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from\u00a0the University of Rochester Medical Center<\/p><p><a href=\"https:\/\/www.urmc.rochester.edu\/labs\/Pryhuber-Lab\/\" target=\"_blank\" rel=\"noopener noreferrer\"><img class=\"lazyload\" decoding=\"async\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27200%27%20height%3D%2748%27%20viewBox%3D%270%200%20200%2048%27%3E%3Crect%20width%3D%27200%27%20height%3D%2748%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2015\/07\/Pryhuber_Lab.jpg\" alt=\"Pryhuber_Lab\" width=\"200\" height=\"48\" \/><\/a><\/p><p style=\"margin: 0px 0px 20px; font-family: 'PT Sans', Arial, Helvetica, sans-serif; text-align: center;\"><span style=\"font-size: 14pt;\"><span style=\"font-weight: bold;\">Immunofluorescence-\u00a0<\/span><span style=\"font-weight: bold;\">\u00a0<\/span><a href=\"http:\/\/www.scbt.com\/datasheet-30216-hop-fl-73-antibody.html\" target=\"_Blank\"><span style=\"font-weight: bold;\">HOPX<\/span><\/a><span style=\"font-weight: bold;\">,<\/span>\u00a0<a href=\"https:\/\/www.rndsystems.com\/products\/human-rage-antibody_af1145\" target=\"_Blank\"><span style=\"font-weight: bold;\">AGER<\/span><\/a><span style=\"font-weight: bold;\">,\u00a0<\/span>and\u00a0<a href=\"http:\/\/www.sigmaaldrich.com\/catalog\/product\/sigma\/a5228?lang=en&amp;region=US\" target=\"_Blank\"><span style=\"font-weight: bold;\">ACTA2<\/span><\/a><\/span><\/p><p style=\"margin: 0px 0px 20px; font-family: 'PT Sans', Arial, Helvetica, sans-serif;\"><span style=\"font-weight: bold;\">\u00a0<\/span><\/p><p style=\"margin: 0px 0px 20px; font-family: 'PT Sans', Arial, Helvetica, sans-serif;\"><span style=\"font-weight: bold;\">Purpose:\u00a0\u00a0<\/span>Stain slides of 7\u00b5m frozen sections of 4-years-old human lung for\u00a0<a href=\"http:\/\/www.scbt.com\/datasheet-30216-hop-fl-73-antibody.html\" target=\"_Blank\"><span style=\"font-weight: bold;\">HOPX<\/span><\/a><span style=\"font-weight: bold;\">,\u00a0<\/span><a href=\"https:\/\/www.rndsystems.com\/products\/human-rage-antibody_af1145\" target=\"_Blank\"><span style=\"font-weight: bold;\">AGER<\/span><\/a><span style=\"font-weight: bold;\">, and\u00a0<\/span><a href=\"http:\/\/www.sigmaaldrich.com\/catalog\/product\/sigma\/a5228?lang=en&amp;region=US\" target=\"_Blank\"><span style=\"font-weight: bold;\">ACTA2<\/span><\/a>\u00a0with antigen retrieval.<\/p><p style=\"margin: 0px 0px 20px; font-family: 'PT Sans', Arial, Helvetica, sans-serif;\"><span style=\"font-weight: bold;\">Day 1<\/span><\/p><ol style=\"font-family: 'PT Sans', Arial, Helvetica, sans-serif;\"><li>For Frozen tissue, rinse 2X in PBS, then 5 min in 4% PFA\/PBS, then rinse 1X in PBS.<\/li><li>Briefly equilibrate slides in Antigen Retrieval Buffer.<\/li><li>Antigen retrieval, pH 6.0 (times will vary according to microwave).<\/li><li>10 mM sodium citrate, pH 6.0, and heat in a microwave at 96<sup>o<\/sup>C.\u00a0*We usually do a series of three runs (6-7 minutes each run) to equal the time\/temp because of evaporation (refill coplin jars with dH<sub>2<\/sub>O).<\/li><li>Microwave according to instructions on microwave.<\/li><li>Cool on countertop, 15 min.<\/li><li>Rinse with dH<sub>2<\/sub>O<\/li><li>1X PBS, 5 min.<\/li><li>Block in 4% Donkey serum\/PBS-T, 2 hours at RT.<\/li><li>For rabbit anti-\u00a0<a href=\"http:\/\/www.scbt.com\/datasheet-30216-hop-fl-73-antibody.html\" target=\"_Blank\"><span style=\"font-weight: bold;\">HOPX<\/span><\/a>\u00a0(<a href=\"http:\/\/www.scbt.com\/datasheet-30216-hop-fl-73-antibody.html\" target=\"_Blank\"><span style=\"font-weight: bold;\">SC-30216<\/span><\/a>, Lot # C2212, Santa Cruz) dilute 1:200, For goat anti-<a href=\"https:\/\/www.rndsystems.com\/products\/human-rage-antibody_af1145\" target=\"_Blank\"><span style=\"font-weight: bold;\">AGER<\/span><\/a>\u00a0(<a href=\"https:\/\/www.rndsystems.com\/products\/human-rage-antibody_af1145\" target=\"_Blank\"><span style=\"font-weight: bold;\">AF1145<\/span><\/a>, R&amp;D Systems) dilute 1:100, for mouse anti-<a href=\"http:\/\/www.sigmaaldrich.com\/catalog\/product\/sigma\/a5228?lang=en&amp;region=US\" target=\"_Blank\"><span style=\"font-weight: bold;\">ACTA2<\/span><\/a>\u00a0(<a href=\"http:\/\/www.sigmaaldrich.com\/catalog\/product\/sigma\/a5228?lang=en%C2%AEion=US\" target=\"_Blank\"><span style=\"font-weight: bold;\">\u03b1 Smooth muscle actin<\/span><\/a>, A5228, Sigma)\u00a0dilute 1:2000 in blocking buffer. Spin down in \u00b5fuge for 10 minutes and incubate on tissue overnight @ 4<sup>o<\/sup>C.<\/li><\/ol><p style=\"margin: 0px 0px 20px; font-family: 'PT Sans', Arial, Helvetica, sans-serif;\"><span style=\"font-weight: bold;\">Day 2<\/span><\/p><ol style=\"font-family: 'PT Sans', Arial, Helvetica, sans-serif;\"><li>Rinse slides in PBS-T 3X, 5 min.<\/li><li>Apply secondary antibody Apply secondary antibody , Donkey Alexa Fluor 488 anti-rabbit IgG (<a href=\"https:\/\/www.lifetechnologies.com\/order\/catalog\/product\/A21206?ICID=search-a21206\" target=\"_Blank\"><span style=\"font-weight: bold;\">A21206<\/span><\/a>, Lot# 1608521, for anti-<a href=\"http:\/\/www.scbt.com\/datasheet-30216-hop-fl-73-antibody.html\" target=\"_Blank\"><span style=\"font-weight: bold;\">HOPX<\/span><\/a>), ), Donkey Alexa Fluor 568 anti-goat IgG (<a href=\"http:\/\/www.lifetechnologies.com\/order\/catalog\/product\/A11057?ICID=search-a11057\" target=\"_Blank\"><span style=\"font-weight: bold;\">A11057<\/span><\/a>, Lot# 1485187, for anti-<a href=\"https:\/\/www.rndsystems.com\/products\/human-rage-antibody_af1145\" target=\"_Blank\"><span style=\"font-weight: bold;\">AGER<\/span><\/a>), Donkey Alexa Fluor 647 anti-mouse IgG (<a href=\"https:\/\/www.lifetechnologies.com\/order\/catalog\/product\/A31571?ICID=search-a31571\" target=\"_Blank\"><span style=\"font-weight: bold;\">A31571<\/span><\/a>, Lot# 1549801, anti-<a href=\"http:\/\/www.sigmaaldrich.com\/catalog\/product\/sigma\/a5228?lang=en&amp;region=US\" target=\"_Blank\"><span style=\"font-weight: bold;\">ACTA2<\/span><\/a>) at 1:200, in blocking buffer. Spin down in \u00b5fuge for 10 min, apply to tissue and incubated at room temperature for 1 hour.<\/li><li>Rinse in PBS-T 3X, 5 min.<\/li><li>Dilute DAPI 1:2000 and apply to slides for 10 min.<\/li><li>Wash in PBS-T 3X, 5 min.<\/li><li>Rinse slides in 0.1M PB or TB, 3X, 5 min.<\/li><li>Add 1 drop of Prolong Gold anti-fade mounting medium (P36930).<\/li><li>Coverslip with Gold Seal Coverslip (Cat# 3422 Electron Microscopy Sciences, 22 X 22 mm).<\/li><li>Allow Prolong Gold to cure overnight at room temp in light sealed box.<\/li><\/ol><ol style=\"font-family: 'PT Sans', Arial, Helvetica, sans-serif;\" start=\"10\"><li>Store slides in light sealed box @ 4<sup>o<\/sup>C<\/li><\/ol><p style=\"margin: 0px 0px 20px; font-family: 'PT Sans', Arial, Helvetica, sans-serif;\"><span style=\"font-weight: bold;\">\u00a0<\/span><\/p><p style=\"margin: 0px 0px 20px; font-family: 'PT Sans', Arial, Helvetica, sans-serif;\"><span style=\"font-weight: bold;\">Tissue Used:<br \/><\/span><span style=\"font-weight: bold; letter-spacing: 0px;\">LMH-15-D007-LLL-5C4.4<br \/><\/span><span style=\"font-weight: bold; letter-spacing: 0px;\">Gender: Male<br \/><\/span><span style=\"font-weight: bold; letter-spacing: 0px;\">Age: 4 Years<\/span><\/p><p style=\"margin: 0px 0px 20px; font-family: 'PT Sans', Arial, Helvetica, sans-serif;\"><span style=\"font-weight: bold;\">\u00a0 \u00a0 \u00a0 \u00a0 \u00a0\u00a0<\/span><\/p><p style=\"margin: 0px 0px 20px; font-family: 'PT Sans', Arial, Helvetica, sans-serif;\"><span style=\"font-weight: bold;\">Appendix<br \/><\/span><span style=\"font-weight: bold; letter-spacing: 0px;\">Antigen Retrieval Solution<\/span><\/p><p style=\"margin: 0px 0px 20px; font-family: 'PT Sans', Arial, Helvetica, sans-serif;\">18ml Solution A- 0.1M Citric Acid (Sigma, C1909) pH 2.5<\/p><p style=\"margin: 0px 0px 20px; font-family: 'PT Sans', Arial, Helvetica, sans-serif;\">82ml Reagent B- 0.1M Sodium Citrate (Sigma, S4641 ) pH 8.2<\/p><p style=\"margin: 0px 0px 20px; font-family: 'PT Sans', Arial, Helvetica, sans-serif;\">1L dH<sub>2<\/sub>O<\/p><p style=\"margin: 0px 0px 20px; font-family: 'PT Sans', Arial, Helvetica, sans-serif;\">pH 6.0<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-d628d53 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"d628d53\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-676424f6\" data-id=\"676424f6\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-d3ebdcd elementor-widget elementor-widget-facebook-comments\" data-id=\"d3ebdcd\" data-element_type=\"widget\" data-widget_type=\"facebook-comments.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-facebook-widget fb-comments\" data-href=\"https:\/\/research.cchmc.org\/lungimage?p=8347\" data-width=\"100%\" data-numposts=\"10\" data-order-by=\"social\" style=\"min-height: 1px\"><\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-7770bb65\" data-id=\"7770bb65\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-3c1457fb elementor-widget elementor-widget-sidebar\" data-id=\"3c1457fb\" data-element_type=\"widget\" data-widget_type=\"sidebar.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div id=\"search-49\" class=\"widget widget_search\">\t\t<form role=\"search\" class=\"searchform fusion-search-form  fusion-search-form-classic\" method=\"get\" action=\"https:\/\/research.cchmc.org\/lungimage\/\">\n\t\t\t<div class=\"fusion-search-form-content\">\n\n\t\t\t\t\n\t\t\t\t<div class=\"fusion-search-field search-field\">\n\t\t\t\t\t<label><span class=\"screen-reader-text\">Search for:<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<input type=\"search\" value=\"\" name=\"s\" class=\"s\" placeholder=\"Search...\" required aria-required=\"true\" aria-label=\"Search...\"\/>\n\t\t\t\t\t\t\t\t\t\t\t<\/label>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"fusion-search-button search-button\">\n\t\t\t\t\t<input type=\"submit\" class=\"fusion-search-submit searchsubmit\" aria-label=\"Search\" value=\"&#xf002;\" \/>\n\t\t\t\t\t\t\t\t\t<\/div>\n\n\t\t\t\t\n\t\t\t<\/div>\n\n\n\t\t\t\n\t\t<\/form>\n\t\t<\/div><div id=\"text-37\" class=\"widget widget_text\"><div class=\"heading\"><h4 class=\"widget-title\">Related Experiments<\/h4><\/div>\t\t\t<div class=\"textwidget\"><div class=\"wondercarousellightbox_options\" data-skinsfoldername=\"\"  data-jsfolder=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/plugins\/wonderplugin-carousel\/engine\/\" style=\"display:none;\"><\/div>\r\n<div class=\"wonderplugincarousel-container\" id=\"wonderplugincarousel-container-157\"><div class=\"wonderplugincarousel\" id=\"wonderplugincarousel-157\" data-carouselid=\"157\" data-width=\"250\" data-height=\"250\" data-skin=\"vertical\" data-showimgtitle=\"false\" data-sameheight=\"false\" data-sameheightresponsive=\"false\" data-fullwidth=\"false\" data-centerimage=\"false\" data-fitimage=\"false\" data-fitcenterimage=\"false\" data-fixaspectratio=\"false\" data-autoplay=\"true\" data-random=\"false\" data-circular=\"false\" data-pauseonmouseover=\"true\" data-continuous=\"false\" data-responsive=\"true\" data-showhoveroverlay=\"true\" data-showhoveroverlayalways=\"false\" data-hidehoveroverlayontouch=\"false\" data-lightboxresponsive=\"true\" data-lightboxshownavigation=\"false\" data-lightboxnogroup=\"false\" data-lightboxshowtitle=\"true\" data-lightboxshowdescription=\"false\" data-lightboxaddsocialmedia=\"false\" data-usescreenquery=\"false\" data-donotinit=\"false\" data-addinitscript=\"false\" data-doshortcodeontext=\"false\" data-lightboxshowsocial=\"false\" data-lightboxshowfacebook=\"true\" data-lightboxshowtwitter=\"true\" data-lightboxshowpinterest=\"true\" data-lightboxsocialrotateeffect=\"true\" data-donotcircularforless=\"false\" data-hidecontainerbeforeloaded=\"false\" data-hidecontaineroninit=\"true\" data-lightboximagekeepratio=\"true\" data-showplayvideo=\"true\" data-triggerresize=\"false\" data-lightboxfullscreenmode=\"false\" data-lightboxcloseonoverlay=\"true\" data-lightboxvideohidecontrols=\"false\" data-lightboxautoslide=\"false\" data-lightboxshowtimer=\"true\" data-lightboxshowplaybutton=\"true\" data-lightboxalwaysshownavarrows=\"false\" data-lightboxshowtitleprefix=\"true\" data-spacing=\"12\" data-rownumber=\"1\" data-visibleitems=\"4\" data-arrowstyle=\"always\" data-arrowimage=\"arrows-32-32-4.png\" data-arrowwidth=\"32\" data-arrowheight=\"32\" data-navstyle=\"none\" data-navimage=\"bullet-24-24-0.png\" data-navwidth=\"24\" data-navheight=\"24\" data-navspacing=\"4\" data-hoveroverlayimage=\"hoveroverlay-64-64-4.png\" data-lightboxthumbwidth=\"90\" data-lightboxthumbheight=\"60\" data-lightboxthumbtopmargin=\"12\" data-lightboxthumbbottommargin=\"4\" data-lightboxbarheight=\"64\" data-lightboxtitlebottomcss=\"{color:#002e9b; font-size:14px; font-family:Armata,sans-serif,Arial; overflow:hidden; text-align:center;}\" data-lightboxdescriptionbottomcss=\"{color:#333; font-size:12px; font-family:Arial,Helvetica,sans-serif; overflow:hidden; text-align:left; margin:4px 0px 0px; padding: 0px;}\" data-continuousduration=\"2500\" data-autoplaydir=\"left\" data-scrollmode=\"page\" data-interval=\"3000\" data-transitionduration=\"1000\" data-lightboxtitlestyle=\"bottom\" data-lightboximagepercentage=\"75\" data-lightboxdefaultvideovolume=\"1\" data-lightboxoverlaybgcolor=\"#000\" data-lightboxoverlayopacity=\"0.9\" data-lightboxbgcolor=\"#fff\" data-lightboxtitleprefix=\"%NUM \/ %TOTAL\" data-lightboxtitleinsidecss=\"color:#fff; font-size:16px; font-family:Arial,Helvetica,sans-serif; overflow:hidden; text-align:left;\" data-lightboxdescriptioninsidecss=\"color:#fff; font-size:12px; font-family:Arial,Helvetica,sans-serif; overflow:hidden; text-align:left; margin:4px 0px 0px; padding: 0px;\" data-playvideoimage=\"playvideo-64-64-0.png\" data-playvideoimagepos=\"center\" data-imgtitle=\"title\" data-circularlimit=\"3\" data-lightboxsocialposition=\"position:absolute;top:100%;right:0;\" data-lightboxsocialpositionsmallscreen=\"position:absolute;top:100%;right:0;left:0;\" data-lightboxsocialdirection=\"horizontal\" data-lightboxsocialbuttonsize=\"32\" data-lightboxsocialbuttonfontsize=\"18\" data-sameheightmediumscreen=\"769\" data-sameheightmediumheight=\"200\" data-sameheightsmallscreen=\"415\" data-sameheightsmallheight=\"150\" data-triggerresizedelay=\"100\" data-lightboxslideinterval=\"5000\" data-lightboxtimerposition=\"bottom\" data-lightboxtimercolor=\"#dc572e\" data-lightboxtimeropacity=\"1\" data-lightboxbordersize=\"8\" data-lightboxborderradius=\"0\" data-screenquery='{ \"mobile\": { \"screenwidth\": 600, \"visibleitems\": 1 } }' data-jsfolder=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/plugins\/wonderplugin-carousel\/engine\/\" style=\"display:none;position:relative;margin:0 auto;width:100%;max-width:250px;\" ><div class=\"amazingcarousel-list-container\" style=\"overflow:hidden;\"><ul class=\"amazingcarousel-list\"><li class=\"amazingcarousel-item\"><div class=\"amazingcarousel-item-container\"><div class=\"amazingcarousel-image\">\n\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=8280\" target=\"target=\"_Blank\"\"><img decoding=\"async\" class=\"lazyload amazingcarousel-image-img\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27300%27%20height%3D%27300%27%20viewBox%3D%270%200%20300%20300%27%3E%3Crect%20width%3D%27300%27%20height%3D%27300%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/05\/2016-05-17_LMH-15-D024-LLL-8A2.15_HOPX_AGER_ACTA2-07-1.jpg\" alt=\"4 Month Human Lung HOPX, AGER, and ACTA2 Confocal Imaging\" data-description=\"\" \/><\/a>\n\t<div class=\"amazingcarousel-text\">\n\t\t<div class=\"amazingcarousel-text-bg\"><\/div>\n\t\t<div class=\"amazingcarousel-title\">4 Month Human Lung HOPX, AGER, and ACTA2 Confocal Imaging<\/div>\n\t<\/div>\n<\/div><\/div><\/li><li class=\"amazingcarousel-item\"><div class=\"amazingcarousel-item-container\"><div class=\"amazingcarousel-image\">\n\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=8331\" target=\"target=\"Blank\"\"><img decoding=\"async\" class=\"lazyload amazingcarousel-image-img\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27300%27%20height%3D%27300%27%20viewBox%3D%270%200%20300%20300%27%3E%3Crect%20width%3D%27300%27%20height%3D%27300%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/05\/2016-05-18_LMH-15-D003-LLL-4B3.18_HOPX_AGER_ACTA2-08-1.jpg\" alt=\"9 Month Old Human Lung HOPX, AGER, and ACTA2 Confocal Imaging\" data-description=\"\" \/><\/a>\n\t<div class=\"amazingcarousel-text\">\n\t\t<div class=\"amazingcarousel-text-bg\"><\/div>\n\t\t<div class=\"amazingcarousel-title\">9 Month Old Human Lung HOPX, AGER, and ACTA2 Confocal Imaging<\/div>\n\t<\/div>\n<\/div><\/div><\/li><li class=\"amazingcarousel-item\"><div class=\"amazingcarousel-item-container\"><div class=\"amazingcarousel-image\">\n\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=8347\" target=\"target=\"Blank\"\"><img decoding=\"async\" class=\"lazyload amazingcarousel-image-img\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27300%27%20height%3D%27300%27%20viewBox%3D%270%200%20300%20300%27%3E%3Crect%20width%3D%27300%27%20height%3D%27300%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/05\/2016-05-18_LMH-15-D007-LLL-5C4.4_HOPX_AGER_ACTA2-09-1.jpg\" alt=\"4 Year Old Human Lung HOPX, AGER, and ACTA2 Confocal Imaging\" data-description=\"\" \/><\/a>\n\t<div class=\"amazingcarousel-text\">\n\t\t<div class=\"amazingcarousel-text-bg\"><\/div>\n\t\t<div class=\"amazingcarousel-title\">4 Year Old Human Lung HOPX, AGER, and ACTA2 Confocal Imaging<\/div>\n\t<\/div>\n<\/div><\/div><\/li><li class=\"amazingcarousel-item\"><div class=\"amazingcarousel-item-container\"><div class=\"amazingcarousel-image\">\n\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=21480\" target=\"_blank\"><img decoding=\"async\" class=\"lazyload amazingcarousel-image-img\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%27http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%27%20width%3D%27300%27%20height%3D%27300%27%20viewBox%3D%270%200%20300%20300%27%3E%3Crect%20width%3D%27300%27%20height%3D%27300%27%20fill-opacity%3D%220%22%2F%3E%3C%2Fsvg%3E\" data-orig-src=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2018\/04\/2017-10-05_LMH-17-D086-LLL-8A3.11_HOPX_AGER_ACTA2-09-1.jpg\" alt=\"8 Month-Old Human Lung Immunofluorescence and Confocal Imaging BPD Donor D086 Showing Expressions of HOPX, AGER, and ACTA2\" data-description=\"\" \/><\/a>\n\t<div class=\"amazingcarousel-text\">\n\t\t<div class=\"amazingcarousel-text-bg\"><\/div>\n\t\t<div class=\"amazingcarousel-title\">8 Month-Old Human Lung Immunofluorescence and Confocal Imaging BPD Donor D086 Showing Expressions of HOPX, AGER, and ACTA2<\/div>\n\t<\/div>\n<\/div><\/div><\/li><\/ul><div class=\"amazingcarousel-prev\"><\/div><div class=\"amazingcarousel-next\"><\/div><\/div><div class=\"amazingcarousel-nav\"><\/div><\/div><\/div><script>function wondercarousel_157_appendcss(csscode) {var head=document.head || document.getElementsByTagName(\"head\")[0];var style=document.createElement(\"style\");head.appendChild(style);style.type=\"text\/css\";if (style.styleSheet){style.styleSheet.cssText=csscode;} else {style.appendChild(document.createTextNode(csscode));}};wondercarousel_157_appendcss(\"@import url(https:\/\/fonts.googleapis.com\/css?family=Open+Sans);  #wonderplugincarousel-157 .amazingcarousel-image {\t \tposition: relative; 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.amazingcarousel-socialmedia-button { display: inline-block; margin: 4px; }.amazingcarousel-socialmedia-button a { box-shadow: none; }.amazingcarousel-socialmedia-icon { display:table-cell; width:32px; height:32px; font-size:18px; border-radius:50%; color:#fff; vertical-align:middle; text-align:center; cursor:pointer; padding:0;}.amazingcarousel-socialmedia-rotate { transition: transform .4s ease-in; } .amazingcarousel-socialmedia-rotate:hover { transform: rotate(360deg); }\");<\/script><\/div>\n\t\t<\/div>\n\t\t<div id=\"recent-posts-48\" class=\"widget widget_recent_entries\">\n\t\t<div class=\"heading\"><h4 class=\"widget-title\">Recent Experiments<\/h4><\/div>\n\t\t<ul>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=32066\">4 Year-Old Human Lung KRT14, MUC5B, and TUBA4A Confocal Imaging<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31223\">Alcian Blue Staining from PBR 3653 Patient with Cystic Fibrosis<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31202\">H&#038;E Staining from PBR 3383 Patient with Cystic Fibrosis<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31199\">H&#038;E Staining from PBR 3653 Patient with Cystic Fibrosis<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31196\">H&#038;E Staining of 31 Year-Old Human Donor 10<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31193\">H&#038;E Staining of 27 Year-Old Human Donor 3<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31190\">Alcian Blue Staining of 27 Year-Old Human Donor 3<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31186\">Alcian Blue Staining of 31 Year-Old Human Donor 10<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31182\">Alcian Blue Staining from PBR 3383 Patient with Cystic Fibrosis<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31024\">Alcian Blue Staining of 29 Year-Old Human Donor D175-10C1.21<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31019\">Alcian Blue Staining of 29 Year-Old Human Donor D175-11C4.14<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31016\">Alcian Blue Staining of 29 Year-Old Human Donor D175-11C2.11<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31013\">Alcian Blue Staining of 24 Year-Old Human Donor D071-23C2.19<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31010\">Alcian Blue Staining of 24 Year-Old Human Donor D071-17C2.20<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t\t\t\t<li>\n\t\t\t\t\t<a href=\"https:\/\/research.cchmc.org\/lungimage\/?p=31007\">Alcian Blue Staining of 24 Year-Old Human Donor D071-16C3.27<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t<\/ul>\n\n\t\t<\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>\u00a0\u00a0Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from\u00a0the University of Rochester Medical Center Immunofluorescence-\u00a0\u00a0HOPX,\u00a0AGER,\u00a0and\u00a0ACTA2 \u00a0 Purpose:\u00a0\u00a0Stain slides of 7\u00b5m frozen sections of 4-years-old human lung for\u00a0HOPX,\u00a0AGER, and\u00a0ACTA2\u00a0with antigen retrieval. Day 1 For Frozen tissue, rinse 2X in PBS, then 5 min in 4% PFA\/PBS, then rinse 1X in PBS. Briefly equilibrate slides in Antigen [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":8349,"comment_status":"closed","ping_status":"open","sticky":false,"template":"elementor_header_footer","format":"standard","meta":{"footnotes":""},"categories":[125,275],"tags":[5,108,6,19,129],"class_list":["post-8347","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-confocal_4yo","category-homo-sapiens-confocal","tag-acta2","tag-ager","tag-alpha-smooth-muscle-actin","tag-hopx","tag-rage"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v25.6 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>4 Year-Old Human Lung HOPX, AGER, ACTA2 Confocal Imaging -<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/research.cchmc.org\/lungimage\/?p=8347\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"4 Year-Old Human Lung HOPX, AGER, ACTA2 Confocal Imaging -\" \/>\n<meta property=\"og:description\" content=\"\u00a0\u00a0Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from\u00a0the University of Rochester Medical Center Immunofluorescence-\u00a0\u00a0HOPX,\u00a0AGER,\u00a0and\u00a0ACTA2 \u00a0 Purpose:\u00a0\u00a0Stain slides of 7\u00b5m frozen sections of 4-years-old human lung for\u00a0HOPX,\u00a0AGER, and\u00a0ACTA2\u00a0with antigen retrieval. Day 1 For Frozen tissue, rinse 2X in PBS, then 5 min in 4% PFA\/PBS, then rinse 1X in PBS. Briefly equilibrate slides in Antigen [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/research.cchmc.org\/lungimage\/?p=8347\" \/>\n<meta property=\"article:published_time\" content=\"2016-05-20T14:26:57+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2018-09-27T13:26:37+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/research.cchmc.org\/lungimage\/wp-content\/uploads\/2016\/05\/2016-05-18_LMH-15-D007-LLL-5C4.4_HOPX_AGER_ACTA2-09-1.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"300\" \/>\n\t<meta property=\"og:image:height\" content=\"300\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"kitc5i\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"kitc5i\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" 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