{"id":9490,"date":"2016-08-04T18:12:39","date_gmt":"2016-08-04T18:12:39","guid":{"rendered":"https:\/\/research.cchmc.org\/lungimage\/?p=9490"},"modified":"2019-09-10T18:14:48","modified_gmt":"2019-09-10T18:14:48","slug":"alcian-blue-staining-of-8-year-old-human-lung-d036-rll-14c1-14","status":"publish","type":"post","link":"https:\/\/research.cchmc.org\/lungimage\/?p=9490","title":{"rendered":"Alcian Blue Staining of 8 Year Old Human Lung D036-RLL-14C1.14"},"content":{"rendered":"

\"Alcian<\/a>
Alcian Blue Staining of 8 Year Male Human Lung<\/div>
4X Tile Scan<\/div><\/div>\"Alcian<\/a>
Alcian Blue Staining of 8 Year Male Human Lung<\/div>
4X<\/div><\/div>\"Alcian<\/a>
Alcian Blue Staining of 8 Year Male Human Lung<\/div>
10X<\/div><\/div>\"Alcian<\/a>
Alcian Blue Staining of 8 Year Male Human Lung<\/div>
20X<\/div><\/div>\"Alcian<\/a>
Alcian Blue Staining of 8 Year Male Human Lung<\/div>
40X<\/div><\/div>\"Alcian<\/a>
Alcian Blue Staining of 8 Year Male Human Lung<\/div>
4X<\/div><\/div>\"Alcian<\/a>
Alcian Blue Staining of 8 Year Male Human Lung<\/div>
10X<\/div><\/div>\"Alcian<\/a>
Alcian Blue Staining of 8 Year Male Human Lung<\/div>
20X<\/div><\/div>\"Alcian<\/a>
Alcian Blue Staining of 8 Year Male Human Lung<\/div>
40X<\/div><\/div>\"Alcian<\/a>
Alcian Blue Staining of 8 Year Male Human Lung<\/div>
4X<\/div><\/div>\"Alcian<\/a>
Alcian Blue Staining of 8 Year Male Human Lung<\/div>
10X<\/div><\/div>\"Alcian<\/a>
Alcian Blue Staining of 8 Year Male Human Lung<\/div>
20X<\/div><\/div>\"Alcian<\/a>
Alcian Blue Staining of 8 Year Male Human Lung<\/div>
40X<\/div><\/div><\/div><\/div><\/b><\/p>\n<\/div>
<\/div><\/div><\/div>

Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from\u00a0the University of Rochester Medical Center<\/p>\n

\"Pryhuber_Lab\"<\/a><\/p>\n

    \n
  1. For Frozen tissue, rinsed slides for 2X in PBS, then Incubated slides for 5 min in 4% PFA\/PBS, then rinsed slides 1X in PBS.<\/li>\n
  2. Rinsed slides for 3 minutes in running dH2<\/sub>O.<\/li>\n
  3. Slides were then incubated in 3% Glacial Acetic Acid (S101<\/strong><\/a>, Poly Scientific) for 3 minutes.<\/li>\n
  4. Slides were then incubated in filtered Alcian Blue (S111A<\/strong><\/a>, Poly Scientific) for 30 minutes.<\/li>\n
  5. Wash slides under cold running tap water for 10 minutes.<\/li>\n
  6. Incubate for 2 minutes in Nuclear Fast Red (S248<\/strong><\/a>, Poly Scientific).<\/li>\n
  7. Wash under flowing\u00a0dH2<\/sub>O until excess Nuclear Fast Red is washed away.<\/li>\n
  8. Incubated\u00a0slides in 90% ETOH for 20 dips, repeat in different 90% ETOH.<\/li>\n
  9. Incubated slides in 100% ETOH for 20 dips, repeat in different\u00a0100% ETOH.<\/li>\n
  10. Washed\u00a0slides in 2X in Xylene, followed by 6 more washes in xylene.<\/li>\n
  11. Coverslip\u00a0with Permount. (Permount Toulene Solution UN 1294, Cat #\u00a0SP-15-500<\/strong><\/a>\u00a0Fisher Scientific).<\/li>\n<\/ol>\n

    Tissue Used:<\/strong><\/p>\n

    LMH-16-D036-RLL-14C1.14<\/strong><\/p>\n

    Gender: Male<\/strong><\/p>\n

    Age: 8 Years<\/strong><\/p>\n<\/div>

    <\/div><\/div><\/div><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[102,103],"tags":[128],"class_list":["post-9490","post","type-post","status-publish","format-standard","hentry","category-alcianblue","category-postnatal","tag-alcian-blue"],"yoast_head":"\nAlcian Blue Staining of 8 Year Old Human Lung D036-RLL-14C1.14 -<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/research.cchmc.org\/lungimage\/?p=9490\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Alcian Blue Staining of 8 Year Old Human Lung D036-RLL-14C1.14 -\" \/>\n<meta property=\"og:url\" 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