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Hierarchical clustering of 153 single cells from Normal BU3 iPSCs (n = 84) and Cystic fibrosis patient-specific (n = 69) reveals 4 major cell types (CC1–CC4), termed as Mitotic (CC1), Pre-mitotic (CC2), NKX2-1- (CC3), and NKX2-1+ (CC4).
CC1 was labeled “mitotic”
and CC2 was labeled “pre-mitotic” based on slightly lower expression levels of
these markers in the latter cluster, whereas CC3 and CC4 did not appear to
be in active cycle at the time of cell capture for analysis. Importantly NKX2-1+
cells clustered together (CC4) regardless of whether they were sorted GFP+
C17 iPSCs or unsorted BU3 iPSCs.