Alveolar Gas ExchangeArtboard 2@2x


Alcian Blue Staining of 14 Month-Old Human Lung from Donor D072-RLL-13B2

Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from the University of Rochester Medical Center


  1. Paraffin sections were placed at 60oC overnight to melt paraffin.
  2. Paraffin sections were then placed in xylene (Xylenes Reagent Grade ACS Histology/Lab Grade Cat # 399000000 Pharma-AAPER) 3X for 10 minutes each.
  3. Rinsed slides for 3 minutes in running dH2O.
  4. Slides were then incubated in 3% Glacial Acetic Acid (S101, Poly Scientific) for 3 minutes.
  5. Slides were then incubated in filtered Alcian Blue (S111A, Poly Scientific) for 30 minutes.
  6. Wash slides under cold running tap water for 10 minutes.
  7. Incubate for 2 minutes in Nuclear Fast Red (S248, Poly Scientific).
  8. Wash under flowing dH2O until excess Nuclear Fast Red is washed away.
  9. Incubated slides in 90% ETOH for 20 dips, repeat in different 90% ETOH.
  10. Incubated slides in 100% ETOH for 20 dips, repeat in different 100% ETOH.
  11. Washed slides in 2X in Xylene, followed by 6 more washes in xylene.
  12. Coverslip with Permount. (Permount Toulene Solution UN 1294, Cat # SP-15-500 Fisher Scientific).


Tissue Used:
Gender: Female
Age: 14 Months