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Fetal Monkey Lung H&E LMR14.8.6.3 Gestational Day 150

Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from the University of Rochester Medical Center

Pryhuber_Lab

  1. Paraffin sections were placed at 60oC overnight to melt paraffin.
  2. Paraffin sections were then placed in xylene (Xylenes Reagent Grade ACS Histology/Lab Grade Cat # 399000000 Pharma-AAPER) 3X for 10 minutes each.
  3. Rinsed slides in a series of 100% ETOH washes. 20 dips for the first wash, 3 minutes for the second wash, and 20 dips for the third wash.
  4. Rinsed slides in a series of 95% ETOH washes. 20 dips for the first wash, 3 minutes for the second wash, and 20 dips for the third wash.
  5. Rinsed slides in 70% ETOH for 3 min.
  6. Rinsed slides for 3 minutes in running dH2O.
  7. Slides were then incubated in 3% Glacial Acetic Acid (S101, Poly Scientific) for 3 minutes.
  8. Slides were then incubated in filtered Alcian Blue (S111A, Poly Scientific) for 30 minutes.
  9. Wash slides under cold running tap water for 10 minutes.
  10. Incubate for 2 minutes in Nuclear Fast Red (S248, Poly Scientific).
  11. Wash under flowing dH2O until excess Nuclear Fast Red is washed away.
  12. Incubated slides in 90% ETOH for 20 dips, repeat in different 90% ETOH.
  13. Incubated slides in 100% ETOH for 20 dips, repeat in different 100% ETOH.
  14. Washed slides in 2X in Xylene, followed by 6 more washes in xylene.
  15. Coverslip with Permount. (Permount Toulene Solution UN 1294, Cat # SP-15-500 Fisher Scientific).

Tissue Used:

LMR14.8.6.3

By |2019-09-10T19:00:40+00:00April 11th, 2018|Categories: Fetal Monkey, GA 150, H&E|Tags: , |0 Comments

Fetal Monkey Lung H&E LMR14.8.4.3 Gestational Day 150

Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from the University of Rochester Medical Center

Pryhuber_Lab

  1. Paraffin sections were placed at 60oC overnight to melt paraffin.
  2. Paraffin sections were then placed in xylene (Xylenes Reagent Grade ACS Histology/Lab Grade Cat # 399000000 Pharma-AAPER) 3X for 10 minutes each.
  3. Rinsed slides in a series of 100% ETOH washes. 20 dips for the first wash, 3 minutes for the second wash, and 20 dips for the third wash.
  4. Rinsed slides in a series of 95% ETOH washes. 20 dips for the first wash, 3 minutes for the second wash, and 20 dips for the third wash.
  5. Rinsed slides in 70% ETOH for 3 min.
  6. Rinsed slides for 3 minutes in running dH2O.
  7. Slides were then incubated in 3% Glacial Acetic Acid (S101, Poly Scientific) for 3 minutes.
  8. Slides were then incubated in filtered Alcian Blue (S111A, Poly Scientific) for 30 minutes.
  9. Wash slides under cold running tap water for 10 minutes.
  10. Incubate for 2 minutes in Nuclear Fast Red (S248, Poly Scientific).
  11. Wash under flowing dH2O until excess Nuclear Fast Red is washed away.
  12. Incubated slides in 90% ETOH for 20 dips, repeat in different 90% ETOH.
  13. Incubated slides in 100% ETOH for 20 dips, repeat in different 100% ETOH.
  14. Washed slides in 2X in Xylene, followed by 6 more washes in xylene.
  15. Coverslip with Permount. (Permount Toulene Solution UN 1294, Cat # SP-15-500 Fisher Scientific).

Tissue Used:

LMR14.8.4.3

By |2019-09-10T19:00:07+00:00April 11th, 2018|Categories: Fetal Monkey, GA 150, H&E|Tags: , |0 Comments

Fetal Monkey Lung H&E LMR14.10.4.3 Gestational Day 150

Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from the University of Rochester Medical Center

Pryhuber_Lab

  1. Paraffin sections were placed at 60oC overnight to melt paraffin.
  2. Paraffin sections were then placed in xylene (Xylenes Reagent Grade ACS Histology/Lab Grade Cat # 399000000 Pharma-AAPER) 3X for 10 minutes each.
  3. Rinsed slides in a series of 100% ETOH washes. 20 dips for the first wash, 3 minutes for the second wash, and 20 dips for the third wash.
  4. Rinsed slides in a series of 95% ETOH washes. 20 dips for the first wash, 3 minutes for the second wash, and 20 dips for the third wash.
  5. Rinsed slides in 70% ETOH for 3 min.
  6. Rinsed slides for 3 minutes in running dH2O.
  7. Slides were then incubated in 3% Glacial Acetic Acid (S101, Poly Scientific) for 3 minutes.
  8. Slides were then incubated in filtered Alcian Blue (S111A, Poly Scientific) for 30 minutes.
  9. Wash slides under cold running tap water for 10 minutes.
  10. Incubate for 2 minutes in Nuclear Fast Red (S248, Poly Scientific).
  11. Wash under flowing dH2O until excess Nuclear Fast Red is washed away.
  12. Incubated slides in 90% ETOH for 20 dips, repeat in different 90% ETOH.
  13. Incubated slides in 100% ETOH for 20 dips, repeat in different 100% ETOH.
  14. Washed slides in 2X in Xylene, followed by 6 more washes in xylene.
  15. Coverslip with Permount. (Permount Toulene Solution UN 1294, Cat # SP-15-500 Fisher Scientific).

Tissue Used:

LMR14.10.4.3

By |2019-09-10T18:59:27+00:00April 10th, 2018|Categories: Fetal Monkey, GA 150, H&E|Tags: , |0 Comments

Fetal Monkey Lung H&E LMR14.6.6.3 Gestational Day 130

  1. Paraffin sections were placed at 60oC overnight to melt paraffin.
  2. Paraffin sections were then placed in xylene (Xylenes Reagent Grade ACS Histology/Lab Grade Cat # 399000000 Pharma-AAPER) 3X for 10 minutes each.
  3. Rinsed slides in a series of 100% ETOH washes. 20 dips for the first wash, 3 minutes for the second wash, and 20 dips for the third wash.
  4. Rinsed slides in a series of 95% ETOH washes. 20 dips for the first wash, 3 minutes for the second wash, and 20 dips for the third wash.
  5. Rinsed slides in 70% ETOH for 3 min.
  6. Rinsed slides for 3 minutes in running dH2O.
  7. Slides were then incubated in 3% Glacial Acetic Acid (S101, Poly Scientific) for 3 minutes.
  8. Slides were then incubated in filtered Alcian Blue (S111A, Poly Scientific) for 30 minutes.
  9. Wash slides under cold running tap water for 10 minutes.
  10. Incubate for 2 minutes in Nuclear Fast Red (S248, Poly Scientific).
  11. Wash under flowing dH2O until excess Nuclear Fast Red is washed away.
  12. Incubated slides in 90% ETOH for 20 dips, repeat in different 90% ETOH.
  13. Incubated slides in 100% ETOH for 20 dips, repeat in different 100% ETOH.
  14. Washed slides in 2X in Xylene, followed by 6 more washes in xylene.
  15. Coverslip with Permount. (Permount Toulene Solution UN 1294, Cat # SP-15-500 Fisher Scientific).

Tissue Used:

LMR14.6.6.3

By |2019-09-10T18:58:38+00:00April 10th, 2018|Categories: Fetal Monkey, GA130, H&E|Tags: , |0 Comments

Fetal Monkey Lung H&E LMR14.6.4.3 Gestational Day 130

  1. Paraffin sections were placed at 60oC overnight to melt paraffin.
  2. Paraffin sections were then placed in xylene (Xylenes Reagent Grade ACS Histology/Lab Grade Cat # 399000000 Pharma-AAPER) 3X for 10 minutes each.
  3. Rinsed slides in a series of 100% ETOH washes. 20 dips for the first wash, 3 minutes for the second wash, and 20 dips for the third wash.
  4. Rinsed slides in a series of 95% ETOH washes. 20 dips for the first wash, 3 minutes for the second wash, and 20 dips for the third wash.
  5. Rinsed slides in 70% ETOH for 3 min.
  6. Rinsed slides for 3 minutes in running dH2O.
  7. Slides were then incubated in 3% Glacial Acetic Acid (S101, Poly Scientific) for 3 minutes.
  8. Slides were then incubated in filtered Alcian Blue (S111A, Poly Scientific) for 30 minutes.
  9. Wash slides under cold running tap water for 10 minutes.
  10. Incubate for 2 minutes in Nuclear Fast Red (S248, Poly Scientific).
  11. Wash under flowing dH2O until excess Nuclear Fast Red is washed away.
  12. Incubated slides in 90% ETOH for 20 dips, repeat in different 90% ETOH.
  13. Incubated slides in 100% ETOH for 20 dips, repeat in different 100% ETOH.
  14. Washed slides in 2X in Xylene, followed by 6 more washes in xylene.
  15. Coverslip with Permount. (Permount Toulene Solution UN 1294, Cat # SP-15-500 Fisher Scientific).

Tissue Used:

LMR14.6.4.3

By |2019-09-10T18:58:09+00:00April 9th, 2018|Categories: Fetal Monkey, GA130