Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from the University of Rochester Medical Center
Immunofluorescence- HSPA5, ABCA3, and ACTA2
Purpose: Stain slides of 5µmparaffin sections of human lung provided by the HTC for HSPA5, ABCA3, and ACTA2 with antigen retrieval.
- Paraffin sections are placed at 60oC for 2 hours to overnight to melt paraffin.
- Paraffin sections are then placed in xylene 3X for 10 minutes each, followed by 3X in 100% ETOH for 3 minutes each, 95% ETOH for 3 minutes each, and 70% ETOH for 3 minutes each. Slides are then placed in 1X PBS for 5 minutes to completely rehydrate tissue.
- Antigen retrieval, pH 6.0 (times will vary according to mic