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9 Month-Old Human Lung HOPX, AGER, ACTA2 Confocal Imaging

  Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from the University of Rochester Medical Center

Pryhuber_Lab

Immunofluorescence-  HOPX, AGERand ACTA2

Purpose:  Stain slides of 7µm frozen sections of 9-month-old human lung for HOPXAGER, and ACTA2 with antigen retrieval.

Day 1

  1. For Frozen tissue, rinse 2X in PBS, then 5 min in 4% PFA/PBS, then rinse 1X in PBS.
  2. Briefly equilibrate slides in Anti