Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from the University of Rochester Medical Center
Immunofluorescence- HOPX, AGER, and ACTA2
Purpose: Stain slides of 7µm frozen sections of 9-month-old human lung for HOPX, AGER, and ACTA2 with antigen retrieval.
- For Frozen tissue, rinse 2X in PBS, then 5 min in 4% PFA/PBS, then rinse 1X in PBS.
- Briefly equilibrate slides in Anti