Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from the University of Rochester Medical Center
Immunofluorescence- SFTPC, ABCA3, and ACTA2
Purpose: Stain slides of 5µm paraffin sections of human lung kindly provided by Dr. Gloria Pryhuber from the University of Rochester Medical Center for SFTPC, ABCA3, and ACTA2 with antigen retrieval.
Day 1
Day 2
Tissue Used:
LMH-16-D034-RLL-7A2.26
Gender: Male
Age: 6 Months
Appendix
Antigen Retrieval Solution
18ml Solution A- 0.1M Citric Acid (Sigma, C1909) pH 2.5
82ml Reagent B- 0.1M Sodium Citrate (Sigma, S4641 ) pH 8.2
1L dH2O
pH 6.0
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Embryonic
3-7 Weeks PC Human
E9.5-12 Mouse
The Embryonic stage of lung development occurs from 4-7 weeks PC in human and embryonic (E) day 9.5-E12 in mouse (Ref). During the embryonic period, trachea, main stem, lobar and segmental bronchi are formed and the trachea and esophagus separate.
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Gene | Cell Type(s) | Subcellular Localization | LungGENS | Mouse LungImage | Ensembl | Genecards | NCBI | Human Protein Atlas | Wikipedia |
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Acta2 | smooth muscle cell | cytoskeleton / microfilaments | Acta2 E16.5 | E16.5 | Ensembl | Genecards | NCBI | The Human Protein Atlas | Wikipedia |
alpha-actin-2 | (conducting airways; trachea, bronchi, bronchioles) | Acta2 E18.5 | E18.5 | ||||||
syn: a-SMA, alpha-smooth muscle actin | (pulmonary blood vessels) | Acta2 P01 | |||||||
*cytoskeletal protein | myofibroblast | Acta2 P03 | |||||||
(pre-alveoar and alveolar parenchyma) | Acta2 P07 | ||||||||
Acta2 P10 | |||||||||
Acta2 P14 | |||||||||
Acta2 P28 |
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