Postnatal Day 3 C57BL6 ARL13B, NKX2.1, and ACTA2 Confocal Imaging
Post Natal Day 3 Immunofluorescence and Confocal Imaging Showing Expression of ARL13B, NKX2.1, and ACTA2
20X
Post Natal Day 3 Immunofluorescence and Confocal Imaging Showing Expression of ARL13B, NKX2.1, and ACTA2
60X
Post Natal Day 3 Immunofluorescence and Confocal Imaging Showing Expression of ARL13B, NKX2.1, and ACTA2
60X
Post Natal Day 3 Immunofluorescence and Confocal Imaging Showing Expression of ARL13B, NKX2.1, and ACTA2
20X
Post Natal Day 3 Immunofluorescence and Confocal Imaging Showing Expression of ARL13B, NKX2.1, and ACTA2
60X
Post Natal Day 3 Immunofluorescence and Confocal Imaging Showing Expression of ARL13B, NKX2.1, and ACTA2
60X
Post Natal Day 3 Immunofluorescence and Confocal Imaging Showing Expression of ARL13B, NKX2.1, and ACTA2
20X
Post Natal Day 3 Immunofluorescence and Confocal Imaging Showing Expression of ARL13B, NKX2.1, and ACTA2
60X
Post Natal Day 3 Immunofluorescence and Confocal Imaging Showing Expression of ARL13B, NKX2.1, and ACTA2
60X
Post Natal Day 3 Immunofluorescence and Confocal Imaging Showing Expression of ARL13B, NKX2.1, and ACTA2
20X
Post Natal Day 3 Immunofluorescence and Confocal Imaging Showing Expression of ARL13B, NKX2.1, and ACTA2
60X
Post Natal Day 3 Immunofluorescence and Confocal Imaging Showing Expression of ARL13B, NKX2.1, and ACTA2
60X
Post Natal Day 3 Immunofluorescence and Confocal Imaging Showing Expression of ARL13B, NKX2.1, and ACTA2
20X
Post Natal Day 3 Immunofluorescence and Confocal Imaging Showing Expression of ARL13B, NKX2.1, and ACTA2
60X
Post Natal Day 3 Immunofluorescence and Confocal Imaging Showing Expression of ARL13B, NKX2.1, and ACTA2
60X
Immunofluorescence-ARL13B, NKX2.1, and ACTA2
Purpose: Immunofluorescence on slides of 7µm frozen sections of C57BL/6 PND03 lungs for ARL13B, NKX2.1, and ACTA2 with antigen retrieval.
Day 1
- For Frozen tissue, rinse 2X in PBS, then 5 min in 4% PFA/PBS, then rinse 1X in PBS.
- Briefly equilibrate slides in Antigen Retrieval Buffer.
- Antigen retrieval, pH 6.0 (times will vary according to microwave).
- 10 mM sodium citrate, pH 6.0, and heat in a microwave at 96oC. *We usually do a series of three runs (6-7 minutes each run) to equal the time/temp because of evaporation (refill coplin jars with dH2O).
- Mi