Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from the University of Rochester Medical Center
Immunofluorescence-NKX2.1, Ki-67 and ACTA2
Purpose: Immunofluorescence of 7 µm frozen section of 3 year-old human lung provided by the HTC for NKX2.1, Ki-67 and ACTA2 with antigen retrieval.
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Day 2
Tissue Used:
LMH-16-D053-RLL-13B2.23
Gender: Male
Age: 3 years
Gestational Age at Birth: 24 weeks
Appendix
Antigen Retrieval Solution
18ml Solution A- 0.1M Citric Acid (Sigma, C1909) pH 2.5
82ml Reagent B- 0.1M Sodium Citrate (Sigma, S4641 ) pH 8.2
1L dH2O
pH 6.0
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Embryonic
3-7 Weeks PC Human
E9.5-12 Mouse
The Embryonic stage of lung development occurs from 4-7 weeks PC in human and embryonic (E) day 9.5-E12 in mouse (Ref). During the embryonic period, trachea, main stem, lobar and segmental bronchi are formed and the trachea and esophagus separate.
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Gene | Cell Type(s) | Subcellular Localization | LungGENS | Mouse LungImage | Ensembl | Genecards | NCBI | Human Protein Atlas | Wikipedia |
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Acta2 | smooth muscle cell | cytoskeleton / microfilaments | Acta2 E16.5 | E16.5 | Ensembl | Genecards | NCBI | The Human Protein Atlas | Wikipedia |
alpha-actin-2 | (conducting airways; trachea, bronchi, bronchioles) | Acta2 E18.5 | E18.5 | ||||||
syn: a-SMA, alpha-smooth muscle actin | (pulmonary blood vessels) | Acta2 P01 | |||||||
*cytoskeletal protein | myofibroblast | Acta2 P03 | |||||||
(pre-alveoar and alveolar parenchyma) | Acta2 P07 | ||||||||
Acta2 P10 | |||||||||
Acta2 P14 | |||||||||
Acta2 P28 |
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