Postnatal Day 10 C57BL6 ARL13B, EMCN, and ACTA2 Confocal Imaging

Immunofluorescence-ARL13B, EMCN, and ACTA2

  Purpose:  Immunofluorescence on slides of 7µm frozen sections of C57BL/6J PND10 lungs forARL13B, EMCN, and ACTA2 with antigen retrieval.

Day 1

                      For Frozen tissue, rinse 2X in PBS, then 5 min in 4% PFA/PBS, then rinse 1X in PBS.

Briefly equilibrate slides in Antigen Retrieval Buffer.

    1. Antigen retrieval, pH 6.0 (times will vary according to