Postnatal Day 7 C57Bl6 NKX2.1, KI67, and ACTA2 Confocal Imaging

Immunofluorescence-NKX2.1, Ki-67 and ACTA2

 

Purpose:  Immunofluorescence on slides of 7µm frozen sections of C57BL/6J PN07 lungs for NKX2.1, Ki-67 and ACTA2 with antigen retrieval.

Day 1

  1. For Frozen tissue, rinse 2X in PBS, then 5 min in 4% PFA/PBS, then rinse 1X in PBS.
  2. Briefly equilibrate slides in Antigen Retrieval Buffer.
  3. Antigen retrieval, pH 6.0 (times will vary according to microwave).
  4. 10 mM sodium citrate, pH 6.0, and heat in a microwave at 96oC. *We usually do a series