Postnatal Day 1 C57BL6 CSPG4, NKX2.1, and ACTA2 Confocal Imaging

Immunofluorescence- CSPG4, NKX2.1, and ACTA2

Purpose:  Immunofluorescence on slides of 7µm frozen sections of C57BL/6J PND01 lungs for CSPG4, NKX2.1, and ACTA2 with antigen retrieval.

Day 1

  1. For Frozen tissue, rinse 2X in PBS, then 5 min in 4% PFA/PBS, then rinse 1X in P