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Alcian Blue Staining of 4 Month-Old Human Lung from Donor D075-LLL-7A2

Alcian Blue Staining of 4 Month-Old Human Lung from Donor D075-LLL-7A2

Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from the University of Rochester Medical Center

Pryhuber_Lab

 

  1. For Frozen tissue, rinsed slides for 2X in PBS, then Incubated slides for 5 min in 4% PFA/PBS, then rinsed slides 1X in PBS.
  2. Rinsed slides for 3 minutes in running dH2O.
  3. Slides were then incubated in 3% Glacial Acetic Acid (S101, Poly Scientific) for 3 minutes.
  4. Slides were then incubated in filtered Alcian Blue (S111A, Poly Scientific) for 30 minutes.
  5. Wash slides under cold running tap water for 10 minutes.
  6. Incubate for 2 minutes in Nuclear Fast Red (S248, Poly Scientific).
  7. Wash under flowing dH2O until excess Nuclear Fast Red is washed away.
  8. Incubated slides in 90% ETOH for 20 dips, repeat in different 90% ETOH.
  9. Incubated slides in 100% ETOH for 20 dips, repeat in different 100% ETOH.
  10. Washed slides in 2X in Xylene, followed by 6 more washes in xylene.
  11. Coverslip with Permount. (Permount Toulene Solution UN 1294, Cat # SP-15-500 Fisher Scientific).

Tissue Used:
LMH-18-D075-LLL-7A2.10
Gender: Male
Age: 4 Months

By |2019-09-10T20:04:46+00:00October 19th, 2018|Categories: 04 Month Old, Alcian Blue, Postnatal Human Lung|0 Comments
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Alcian Blue Staining of 4 Month-Old Human Lung from Donor D075-LLL-5B3

Alcian Blue Staining of 4 Month-Old Human Lung from Donor D075-LLL-5B3

Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from the University of Rochester Medical Center

Pryhuber_Lab

 

  1. For Frozen tissue, rinsed slides for 2X in PBS, then Incubated slides for 5 min in 4% PFA/PBS, then rinsed slides 1X in PBS.
  2. Rinsed slides for 3 minutes in running dH2O.
  3. Slides were then incubated in 3% Glacial Acetic Acid (S101, Poly Scientific) for 3 minutes.
  4. Slides were then incubated in filtered Alcian Blue (S111A, Poly Scientific) for 30 minutes.
  5. Wash slides under cold running tap water for 10 minutes.
  6. Incubate for 2 minutes in Nuclear Fast Red (S248, Poly Scientific).
  7. Wash under flowing dH2O until excess Nuclear Fast Red is washed away.
  8. Incubated slides in 90% ETOH for 20 dips, repeat in different 90% ETOH.
  9. Incubated slides in 100% ETOH for 20 dips, repeat in different 100% ETOH.
  10. Washed slides in 2X in Xylene, followed by 6 more washes in xylene.
  11. Coverslip with Permount. (Permount Toulene Solution UN 1294, Cat # SP-15-500 Fisher Scientific).

Tissue Used:
LMH-18-D075-LLL-5B3.9
Gender: Male
Age: 4 Months

By |2019-09-10T20:04:07+00:00October 19th, 2018|Categories: 04 Month Old, Alcian Blue, Postnatal Human Lung|0 Comments
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Alcian Blue Staining of 4 Month-Old Human Lung from Donor D075-LLL-6A3

Alcian Blue Staining of 4 Month-Old Human Lung from Donor D075-LLL-6A3

 

Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from the University of Rochester Medical Center

Pryhuber_Lab

 

  1. For Frozen tissue, rinsed slides for 2X in PBS, then Incubated slides for 5 min in 4% PFA/PBS, then rinsed slides 1X in PBS.
  2. Rinsed slides for 3 minutes in running dH2O.
  3. Slides were then incubated in 3% Glacial Acetic Acid (S101, Poly Scientific) for 3 minutes.
  4. Slides were then incubated in filtered Alcian Blue (S111A, Poly Scientific) for 30 minutes.
  5. Wash slides under cold running tap water for 10 minutes.
  6. Incubate for 2 minutes in Nuclear Fast Red (S248, Poly Scientific).
  7. Wash under flowing dH2O until excess Nuclear Fast Red is washed away.
  8. Incubated slides in 90% ETOH for 20 dips, repeat in different 90% ETOH.
  9. Incubated slides in 100% ETOH for 20 dips, repeat in different 100% ETOH.
  10. Washed slides in 2X in Xylene, followed by 6 more washes in xylene.
  11. Coverslip with Permount. (Permount Toulene Solution UN 1294, Cat # SP-15-500 Fisher Scientific).

Tissue Used:
LMH-18-D075-LLL-6A3.8
Gender: Male
Age: 4 Months

By |2019-09-10T20:03:21+00:00October 19th, 2018|Categories: 04 Month Old, Alcian Blue, Postnatal Human Lung|0 Comments
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Alcian Blue Staining of 11 Month-Old Human Lung from Donor D094-LLL-6A3

Alcian Blue Staining of 11 Month-Old Human Lung from Donor D094-LLL-6A3

Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from the University of Rochester Medical Center

Pryhuber_Lab

 

  1. For Frozen tissue, rinsed slides for 2X in PBS, then Incubated slides for 5 min in 4% PFA/PBS, then rinsed slides 1X in PBS.
  2. Rinsed slides for 3 minutes in running dH2O.
  3. Slides were then incubated in 3% Glacial Acetic Acid (S101, Poly Scientific) for 3 minutes.
  4. Slides were then incubated in filtered Alcian Blue (S111A, Poly Scientific) for 30 minutes.
  5. Wash slides under cold running tap water for 10 minutes.
  6. Incubate for 2 minutes in Nuclear Fast Red (S248, Poly Scientific).
  7. Wash under flowing dH2O until excess Nuclear Fast Red is washed away.
  8. Incubated slides in 90% ETOH for 20 dips, repeat in different 90% ETOH.
  9. Incubated slides in 100% ETOH for 20 dips, repeat in different 100% ETOH.
  10. Washed slides in 2X in Xylene, followed by 6 more washes in xylene.
  11. Coverslip with Permount. (Permount Toulene Solution UN 1294, Cat # SP-15-500 Fisher Scientific).

 

Tissue Used:
LMH-18-D094-LLL-6A3.7
Gender: Male
Age: 11 Months

By |2019-09-11T12:23:56+00:00October 19th, 2018|Categories: 11 Month-Old, Alcian Blue, Postnatal Human Lung|0 Comments
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Alcian Blue Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2

Alcian Blue Staining of 11 Month-Old Human Lung from Donor D094-LLL-5B2

Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from the University of Rochester Medical Center

Pryhuber_Lab

 

  1. For Frozen tissue, rinsed slides for 2X in PBS, then Incubated slides for 5 min in 4% PFA/PBS, then rinsed slides 1X in PBS.
  2. Rinsed slides for 3 minutes in running dH2O.
  3. Slides were then incubated in 3% Glacial Acetic Acid (S101, Poly Scientific) for 3 minutes.
  4. Slides were then incubated in filtered Alcian Blue (S111A, Poly Scientific) for 30 minutes.
  5. Wash slides under cold running tap water for 10 minutes.
  6. Incubate for 2 minutes in Nuclear Fast Red (S248, Poly Scientific).
  7. Wash under flowing dH2O until excess Nuclear Fast Red is washed away.
  8. Incubated slides in 90% ETOH for 20 dips, repeat in different 90% ETOH.
  9. Incubated slides in 100% ETOH for 20 dips, repeat in different 100% ETOH.
  10. Washed slides in 2X in Xylene, followed by 6 more washes in xylene.
  11. Coverslip with Permount. (Permount Toulene Solution UN 1294, Cat # SP-15-500 Fisher Scientific).

 

 

Tissue Used:
LMH-18-D094-LLL-5B2.4
Gender: Male
Age: 11 Months

By |2019-09-11T12:22:14+00:00October 16th, 2018|Categories: 11 Month-Old, Alcian Blue, Postnatal Human Lung|0 Comments
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Alcian Blue Staining of 11 Month-Old Human Lung from Donor D094-LLL-5A3

Alcian Blue Staining of 11 Month-Old Human Lung from Donor D094-LLL-5A3

Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from the University of Rochester Medical Center

Pryhuber_Lab

 

  1. For Frozen tissue, rinsed slides for 2X in PBS, then Incubated slides for 5 min in 4% PFA/PBS, then rinsed slides 1X in PBS.
  2. Rinsed slides for 3 minutes in running dH2O.
  3. Slides were then incubated in 3% Glacial Acetic Acid (S101, Poly Scientific) for 3 minutes.
  4. Slides were then incubated in filtered Alcian Blue (S111A, Poly Scientific) for 30 minutes.
  5. Wash slides under cold running tap water for 10 minutes.
  6. Incubate for 2 minutes in Nuclear Fast Red (S248, Poly Scientific).
  7. Wash under flowing dH2O until excess Nuclear Fast Red is washed away.
  8. Incubated slides in 90% ETOH for 20 dips, repeat in different 90% ETOH.
  9. Incubated slides in 100% ETOH for 20 dips, repeat in different 100% ETOH.
  10. Washed slides in 2X in Xylene, followed by 6 more washes in xylene.
  11. Coverslip with Permount. (Permount Toulene Solution UN 1294, Cat # SP-15-500 Fisher Scientific).

 

 

Tissue Used:
LMH-18-D094-LLL-5A3.5
Gender: Male
Age: 11 Months

By |2019-09-11T12:21:21+00:00October 16th, 2018|Categories: 11 Month-Old, Alcian Blue, Postnatal Human Lung|0 Comments
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Alcian Blue Staining of 14 Month-Old Human Lung from Donor D072-RLL-14B2

Alcian Blue Staining of 14 Month-Old Human Lung from Donor D072-RLL-14B2

Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from the University of Rochester Medical Center

Pryhuber_Lab

  1. Paraffin sections were placed at 60oC overnight to melt paraffin.
  2. Paraffin sections were then placed in xylene (Xylenes Reagent Grade ACS Histology/Lab Grade Cat # 399000000 Pharma-AAPER) 3X for 10 minutes each.
  3. Rinsed slides for 3 minutes in running dH2O.
  4. Slides were then incubated in 3% Glacial Acetic Acid (S101, Poly Scientific) for 3 minutes.
  5. Slides were then incubated in filtered Alcian Blue (S111A, Poly Scientific) for 30 minutes.
  6. Wash slides under cold running tap water for 10 minutes.
  7. Incubate for 2 minutes in Nuclear Fast Red (S248, Poly Scientific).
  8. Wash under flowing dH2O until excess Nuclear Fast Red is washed away.
  9. Incubated slides in 90% ETOH for 20 dips, repeat in different 90% ETOH.
  10. Incubated slides in 100% ETOH for 20 dips, repeat in different 100% ETOH.
  11. Washed slides in 2X in Xylene, followed by 6 more washes in xylene.
  12. Coverslip with Permount. (Permount Toulene Solution UN 1294, Cat # SP-15-500 Fisher Scientific).

 

Tissue Used:
LMH-18-D072-RLL-14B2.3
Gender: Female
Age: 14 Months

By |2019-09-11T12:31:32+00:00October 16th, 2018|Categories: 14 Month-Old, Alcian Blue, Postnatal Human Lung|0 Comments
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Alcian Blue Staining of 7 Year-Old Human Lung from Donor D078-LLL-12B5

Alcian Blue Staining of 7 Year-Old Human Lung from Donor D078-LLL-12B5

Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from the University of Rochester Medical Center

Pryhuber_Lab

 

  1. For Frozen tissue, rinsed slides for 2X in PBS, then Incubated slides for 5 min in 4% PFA/PBS, then rinsed slides 1X in PBS.
  2. Rinsed slides for 3 minutes in running dH2O.
  3. Slides were then incubated in 3% Glacial Acetic Acid (S101, Poly Scientific) for 3 minutes.
  4. Slides were then incubated in filtered Alcian Blue (S111A, Poly Scientific) for 30 minutes.
  5. Wash slides under cold running tap water for 10 minutes.
  6. Incubate for 2 minutes in Nuclear Fast Red (S248, Poly Scientific).
  7. Wash under flowing dH2O until excess Nuclear Fast Red is washed away.
  8. Incubated slides in 90% ETOH for 20 dips, repeat in different 90% ETOH.
  9. Incubated slides in 100% ETOH for 20 dips, repeat in different 100% ETOH.
  10. Washed slides in 2X in Xylene, followed by 6 more washes in xylene.
  11. Coverslip with Permount. (Permount Toulene Solution UN 1294, Cat # SP-15-500 Fisher Scientific).

 

 

Tissue Used:
LMH-18-D078-LLL-12B5.2
Gender: Female
Age: 7 Years

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By |2018-10-16T17:54:40+00:00October 16th, 2018|Categories: 7 Year-Old, Alcian Blue, Asthma Lung HE Imaging, Postnatal Human Lung|0 Comments
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Alcian Blue Staining of 14 Month-Old Human Lung from Donor D072-LLL-13B4

Alcian Blue Staining of 14 Month-Old Human Lung from Donor D072-LLL-13B4

Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from the University of Rochester Medical Center

Pryhuber_Lab

 

  1. For Frozen tissue, rinsed slides for 2X in PBS, then Incubated slides for 5 min in 4% PFA/PBS, then rinsed slides 1X in PBS.
  2. Rinsed slides for 3 minutes in running dH2O.
  3. Slides were then incubated in 3% Glacial Acetic Acid (S101, Poly Scientific) for 3 minutes.
  4. Slides were then incubated in filtered Alcian Blue (S111A, Poly Scientific) for 30 minutes.
  5. Wash slides under cold running tap water for 10 minutes.
  6. Incubate for 2 minutes in Nuclear Fast Red (S248, Poly Scientific).
  7. Wash under flowing dH2O until excess Nuclear Fast Red is washed away.
  8. Incubated slides in 90% ETOH for 20 dips, repeat in different 90% ETOH.
  9. Incubated slides in 100% ETOH for 20 dips, repeat in different 100% ETOH.
  10. Washed slides in 2X in Xylene, followed by 6 more washes in xylene.
  11. Coverslip with Permount. (Permount Toulene Solution UN 1294, Cat # SP-15-500 Fisher Scientific).

 

 

Tissue Used:
LMH-18-D072-LLL-13B4.1
Gender: Female
Age: 14 Months

By |2019-09-11T12:30:50+00:00October 15th, 2018|Categories: 14 Month-Old, Alcian Blue, Postnatal Human Lung|0 Comments
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Alcian Blue Staining of 14 Month-Old Human Lung from Donor D072-RLL-15B3

Alcian Blue Staining of 14 Month-Old Human Lung from Donor D072-RLL-15B3

Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from the University of Rochester Medical Center

Pryhuber_Lab

  1. Paraffin sections were placed at 60oC overnight to melt paraffin.
  2. Paraffin sections were then placed in xylene (Xylenes Reagent Grade ACS Histology/Lab Grade Cat # 399000000 Pharma-AAPER) 3X for 10 minutes each.
  3. Rinsed slides for 3 minutes in running dH2O.
  4. Slides were then incubated in 3% Glacial Acetic Acid (S101, Poly Scientific) for 3 minutes.
  5. Slides were then incubated in filtered Alcian Blue (S111A, Poly Scientific) for 30 minutes.
  6. Wash slides under cold running tap water for 10 minutes.
  7. Incubate for 2 minutes in Nuclear Fast Red (S248, Poly Scientific).
  8. Wash under flowing dH2O until excess Nuclear Fast Red is washed away.
  9. Incubated slides in 90% ETOH for 20 dips, repeat in different 90% ETOH.
  10. Incubated slides in 100% ETOH for 20 dips, repeat in different 100% ETOH.
  11. Washed slides in 2X in Xylene, followed by 6 more washes in xylene.
  12. Coverslip with Permount. (Permount Toulene Solution UN 1294, Cat # SP-15-500 Fisher Scientific).

 

Tissue Used:
LMH-18-D072-RLL-15B3.16
Gender: Female
Age: 14 Months

By |2019-09-11T12:29:43+00:00October 15th, 2018|Categories: 14 Month-Old, Alcian Blue, Postnatal Human Lung|0 Comments
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