43 Year-Old Human Lung NKX2.1, AGER, and ACTA2 Confocal Imaging

Gene
Nkx2-1	LungGens	Ensemble	Genecards	NCBI	The Human Protein Atlas
AGER	LungGens	Ensemble	Genecards	NCBI	The Human Protein Atlas
Acta2	LungGens	Ensemble	Genecards	NCBI	The Human Protein Atlas

Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from the University of Rochester Medical Center

Immunofluorescence- NKX2.1, AGER, and ACTA2

Purpose: Stain slides of 7µm frozen sections of 43-year-old human lung, courtesy of Dr. Scott Randell, University of North Carolina, for NKX2.1, AGER, and ACTA2 with antigen retrieval.

Day 1

For Frozen tissue, rinse 2X in PBS, then 5 min i