Postnatal Day 10 C57BL6 NKX2.1, SCGB1A1, and TUBB3 Confocal Imaging

Immunofluorescence NKX2.1, SCGB1A1, and TUBB3

 

Purpose:  Stain slides of 7µm frozen sections of C57BL/6J PND10 lung for NKX2.1, SCGB1A1, and TUBB3 with antigen retrieval.

Day 1

  1. For Frozen tissue, rinse 2X in PBS, then 5 min in 4% PFA/PBS, then rinse 1X in PBS.
  2. Bri