Postnatal Day 28 C57BL6 NKX2.1, VIM, and ACTA2 Confocal Imaging

                                                       Immunofluorescence- NKX2.1, VIM and ACTA2

 Purpose:  Stain slides of 7µm frozen sections of C57BL/6J PND10 mouse lungs for NKX2.1VIM, and ACTA2 with antigen retrieval.

 Day 1

  1. For Frozen tissue, rinse 2X in PBS, then 5 min in 4% PFA/PBS, then rinse 1X in PBS.
  2. Briefly equilibrate slides in Antigen Retrieval Buffer.
  3. Antigen retrieval, pH 6.0 (times will vary according to microwave).
  4. 10 mM sodium citrate, pH 6.0, and heat in a microw