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20 Month-Old Human Lung Confocal Imaging Donor D011 KRT14, SOX2, and KRT5

20 Month-Old Human Lung Confocal Imaging Donor D011 KRT14, SOX2, and KRT5

Click here

  Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from the University of Rochester Medical Center

Pryhuber_Lab

Immunofluorescence-KRT14, SOX2 and KRT5

 

Purpose:  Immunofluorescence on slides of frozen sections of control and BPD human lung  from the HTC for KRT14, SOX2 and KRT5 with antigen retrieval.

Day 1

  1. For Frozen tissue, rinse 2X in PBS, then 5 min in 4% PFA/PBS, then rinse 1X in PBS.
  2. Briefly equilibrate slides in Antigen Retrieval Buffer.
  3. Antigen retrieval, pH 6.0 (times will vary according to microwave).
  4. 10 mM sodium citrate, pH 6.0, and heat in a microwave at 96oC. *We usually do a series of three runs (6-7 minutes each run) to equal the time/temp because of evaporation (refill coplin jars with dH2O).
  5. Microwave according to instructions on microwave.
  6. Cool on countertop, 15 min.
  7. Rinse with dH2O
  8. 1X PBS, 5 min.
  9. Block in 4% Goat serum/PBS-T, 2 hours at RT.
  10. For mouse anti-KRT14 (AB7800, Abcam) dilute 1:100, For mouse anti-SOX2 (SC-365823, Santa Cruz, Lot# C0317) dilute 1:100, for rabbit anti-KRT5 (PRB-160P-200, Lot# D13DF01179, Biolegend) Dilute 1:100 in blocking buffer. Spin down in µfuge for 10 minutes and incubate on tissue overnight @ 4oC.

Day 2

  1. Rinse slides in PBS-T 3X, 5 min.
  2. Apply secondary antibody, Goat Alexa Fluor 488 anti-mouse IgG3 (A21151, Lot# 1563682,  for anti-KRT14) at 1:200, Goat Alexa Fluor 568 anti-mouse IgG1 (A21124, Lot# 1600878, for anti-SOX2) at 1:200, Goat Alexa Fluor 633 anti-rabbit IgG (A21070, Lot # 1668691, anti-KRT5) at 1:200, in blocking buffer. Spin down in µfuge for 10 min, apply to tissue and incubated at room temperature for 1 hour.
  3. Rinse in PBS-T 5X
  4. Dilute DAPI 1:2000 and apply to slides for 10 min.
  5. Wash in PBS-T 3X, 5 min.
  6. Rinse slides in 0.1M PB or TB, 3X, 5 min.
  7. Add 1 drop of Prolong Gold anti-fade mounting medium (P36930).
  8. Coverslip with Gold Seal Coverslip (Cat# 3422 Electron Microscopy Sciences, 22 X 22 mm).
  9. Allow Prolong Gold to cure overnight at room temp in light sealed box.
  10. Store in light-sealed box at 4oC.

 

 

Tissue Used:
LMH-16-D011-LLL-7B2.6
Gender: Female
Age: 20 Months

                   

Appendix
Antigen Retrieval Solution

18ml Solution A- 0.1M Citric Acid (Sigma, C1909) pH 2.5

82ml Reagent B- 0.1M Sodium Citrate (Sigma, S4641 ) pH 8.2

1L dH2O

pH 6.0

 

Related Experiments

By |2018-11-12T15:58:13+00:00March 5th, 2018|Categories: 20 Month-Old, Homo sapiens Confocal Imaging|Tags: , , |0 Comments
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20 Month-Old Human Lung Confocal Imaging Donor D008 KRT14, SOX2, and KRT5

20 Month-Old Human Lung Confocal Imaging Donor D008 KRT14, SOX2, and KRT5

Click here

  Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from the University of Rochester Medical Center

Pryhuber_Lab

Immunofluorescence-KRT14, SOX2 and KRT5

 

Purpose:  Immunofluorescence on slides of frozen sections of control and BPD human lung  from the HTC for KRT14, SOX2 and KRT5 with antigen retrieval.

Day 1

  1. For Frozen tissue, rinse 2X in PBS, then 5 min in 4% PFA/PBS, then rinse 1X in PBS.
  2. Briefly equilibrate slides in Antigen Retrieval Buffer.
  3. Antigen retrieval, pH 6.0 (times will vary according to microwave).
  4. 10 mM sodium citrate, pH 6.0, and heat in a microwave at 96oC. *We usually do a series of three runs (6-7 minutes each run) to equal the time/temp because of evaporation (refill coplin jars with dH2O).
  5. Microwave according to instructions on microwave.
  6. Cool on countertop, 15 min.
  7. Rinse with dH2O
  8. 1X PBS, 5 min.
  9. Block in 4% Goat serum/PBS-T, 2 hours at RT.
  10. For mouse anti-KRT14 (AB7800, Abcam) dilute 1:100, For mouse anti-SOX2 (SC-365823, Santa Cruz, Lot# C0317) dilute 1:100, for rabbit anti-KRT5 (PRB-160P-200, Lot# D13DF01179, Biolegend) Dilute 1:100 in blocking buffer. Spin down in µfuge for 10 minutes and incubate on tissue overnight @ 4oC.

Day 2

  1. Rinse slides in PBS-T 3X, 5 min.
  2. Apply secondary antibody, Goat Alexa Fluor 488 anti-mouse IgG3 (A21151, Lot# 1563682,  for anti-KRT14) at 1:200, Goat Alexa Fluor 568 anti-mouse IgG1 (A21124, Lot# 1600878, for anti-SOX2) at 1:200, Goat Alexa Fluor 633 anti-rabbit IgG (A21070, Lot # 1668691, anti-KRT5) at 1:200, in blocking buffer. Spin down in µfuge for 10 min, apply to tissue and incubated at room temperature for 1 hour.
  3. Rinse in PBS-T 5X
  4. Dilute DAPI 1:2000 and apply to slides for 10 min.
  5. Wash in PBS-T 3X, 5 min.
  6. Rinse slides in 0.1M PB or TB, 3X, 5 min.
  7. Add 1 drop of Prolong Gold anti-fade mounting medium (P36930).
  8. Coverslip with Gold Seal Coverslip (Cat# 3422 Electron Microscopy Sciences, 22 X 22 mm).
  9. Allow Prolong Gold to cure overnight at room temp in light sealed box.
  10. Store in light-sealed box at 4oC.

 

 

Tissue Used:
LMH-16-D008-LLL-7B3.25
Gender: Female
Age: 20 Months

                       

 

Appendix
Antigen Retrieval Solution

18ml Solution A- 0.1M Citric Acid (Sigma, C1909) pH 2.5

82ml Reagent B- 0.1M Sodium Citrate (Sigma, S4641 ) pH 8.2

1L dH2O

pH 6.0

Related Experiments

By |2018-11-12T16:00:07+00:00March 5th, 2018|Categories: 20 Month-Old, Homo sapiens Confocal Imaging|Tags: , , |0 Comments
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8 Year-Old Human Lung Confocal Imaging Donor D036 KRT14, SOX2, and KRT5

8 Year-Old Human Lung Confocal Imaging Donor D036 KRT14, SOX2, and KRT5

Click here

  Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from the University of Rochester Medical Center

Pryhuber_Lab

Immunofluorescence-KRT14, SOX2 and KRT5 

Purpose:  Immunofluorescence on slides of frozen sections of control and BPD human lung  from the HTC for KRT14, SOX2 and KRT5 
with antigen retrieval.

Day 1

  1. For Frozen tissue, rinse 2X in PBS, then 5 min in 4% PFA/PBS, then rinse 1X in PBS.
  2. Briefly equilibrate slides in Antigen Retrieval Buffer.
  3. Antigen retrieval, pH 6.0 (times will vary according to microwave).
  4. 10 mM sodium citrate, pH 6.0, and heat in a microwave at 96oC. *We usually do a series of three runs (6-7 minutes each run) to
    equal the time/temp because of evaporation (refill coplin jars with dH2O).
  5. Microwave according to instructions on microwave.
  6. Cool on countertop, 15 min.
  7. Rinse with dH2O
  8. 1X PBS, 5 min.
  9. Block in 4% Goat serum/PBS-T, 2 hours at RT.
  10. For mouse anti-KRT14 (AB7800, Abcam) dilute 1:100, For mouse anti-SOX2 (SC-365823, Santa Cruz, Lot# C0317) dilute 1:100,
    for rabbit anti-KRT5 (PRB-160P-200, Lot# D13DF01179, Biolegend) Dilute 1:100 in blocking buffer. Spin down in µfuge for 10
    minutes and incubate on tissue overnight @ 4oC.

Day 2

  1. Rinse slides in PBS-T 3X, 5 min.
  2. Apply secondary antibody, Goat Alexa Fluor 488 anti-mouse IgG3 (A21151, Lot# 1563682,  for anti-KRT14) at 1:200, Goat
    Alexa Fluor 568 anti-mouse IgG1 (A21124, Lot# 1600878, for anti-SOX2) at 1:200, Goat Alexa Fluor 633 anti-rabbit IgG
    (A21070, Lot # 1668691, anti-KRT5) at 1:200, in blocking buffer. Spin down in µfuge for 10 min, apply to tissue and incubated
    at room temperature for 1 hour.
  3. Rinse in PBS-T 5X
  4. Dilute DAPI 1:2000 and apply to slides for 10 min.
  5. Wash in PBS-T 3X, 5 min.
  6. Rinse slides in 0.1M PB or TB, 3X, 5 min.
  7. Add 1 drop of Prolong Gold anti-fade mounting medium (P36930).
  8. Coverslip with Gold Seal Coverslip (Cat# 3422 Electron Microscopy Sciences, 22 X 22 mm).
  9. Allow Prolong Gold to cure overnight at room temp in light sealed box.
  10. Store in light-sealed box at 4oC.

 

Tissue Used:
LMH-16-D036-LLL-7B2.18
Gender: Male
Age: 8 Years

 

Appendix
Antigen Retrieval Solution

18ml Solution A- 0.1M Citric Acid (Sigma, C1909) pH 2.5

82ml Reagent B- 0.1M Sodium Citrate (Sigma, S4641 ) pH 8.2

1L dH2O

pH 6.0

 
 
 

Related Experiments

By |2018-11-12T16:01:40+00:00March 5th, 2018|Categories: 8 Year-old, Homo sapiens Confocal Imaging|Tags: , , |0 Comments
Read More

4 Month Human Lung TP63, NKX2.1, and KRT5 Confocal Imaging

4 Month Human Lung TP63, NKX2.1, and KRT5 Confocal Imaging

  Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from the University of Rochester Medical Center

Pryhuber_Lab

Immunofluorescence-TP63, NKX2.1 and KRT5

 

Purpose:  Immunofluorescence on slides of human lung from the HTC for TP63, NKX2.1 and KRT5 with antigen retrieval.

Day 1

  1. Paraffin sections are placed on slide warmer for 1 hour to melt paraffin.
  2. Paraffin sections are then placed in xylene 3X for 10 minutes each, followed by 3X in 100% ETOH for 3 minutes each, 95% ETOH for 3 minutes each, and 70% ETOH for 3 minutes each. Slides are then placed in 1X PBS for 5 minutes to completely rehydrate tissue.
  3. Briefly equilibrate slides in Antigen Retrieval Buffer.
  4. Antigen retrieval, pH 6.0 (times will vary according to microwave).
  5. 10 mM sodium citrate, pH 6.0, and heat in a microwave at 96oC. *We usually do a series of three runs (6-7 minutes each run) to equal the time/temp because of evaporation (refill coplin jars with dH2O).
  6. Microwave according to instructions on microwave.
  7. Cool on countertop, 15 min.
  8. Rinse with dH2O
  9. 1X PBS, 5 min.
  10. Block in 4% Goat serum/PBS-T, 2 hours at RT.
  11. For mouse anti-TP63 (CM163A, lot # 102214, Biocare) dilute 1:100, For guinea pig anti-NKX2.1 (G237, Bl. 8/25/11) dilute 1:200 for rabbit KRT5 (PRB-160P-200, Lot# D13DF01179, Covance) Dilute 1:100 in blocking buffer. Spin down in µfuge for 10 minutes and incubate on tissue overnight @ 4oC.

Day 2

  1. Rinse slides in PBS-T 3X, 5 min.
  2. Goat Alexa Fluor 488 anti-mouse IgG (A11001, Lot# 1298479, for anti-TP63) dilute 1:200, Goat Alexa Fluor 555 anti-guinea pig IgG (A21435, Lot# 1571699, for anti-NKX2.1) at 1:200, Goat Alexa Fluor 633 anti-rabbit IgG (A21070, Lot # 1668691, anti-KRT5) at 1:200, in blocking buffer. Spin down in µfuge for 10 min, apply to tissue and incubated at room temperature for 1 hour.
  3. Rinse in PBS-T 3X, 5 min.
  4. Dilute DAPI 1:2000 and apply to slides for 10 min.
  5. Wash in PBS-T 3X, 5 min.
  6. Rinse slides in 0.1M PB or TB, 3X, 5 min.
  7. Add 1 drop of Prolong Gold anti-fade mounting medium (P36930).
  8. Coverslip with Gold Seal Coverslip (Cat# 3422 Electron Microscopy Sciences, 22 X 22 mm).
  9. Allow Prolong Gold to cure overnight at room temp in light sealed box.

 

Tissue Used:
LMH-16-D024-RLL-6B1.19
Age: 4 Months
Gender: Male

 

Appendix
Antigen Retrieval Solution

18ml Solution A- 0.1M Citric Acid (Sigma, C1909) pH 2.5

82ml Reagent B- 0.1M Sodium Citrate (Sigma, S4641 ) pH 8.2

1L dH2O

pH 6.0

Related Experiments

By |2018-11-12T14:43:48+00:00April 15th, 2016|Categories: 04 Month-Old, Postnatal Human Lung|Tags: , , , |0 Comments
Read More

4 Year Old Human Lung TP63, NKX2.1, and KRT5 Immunofluorescence and Confocal Imaging

4 Year Old Human Lung TP63, NKX2.1, and KRT5 Immunofluorescence and Confocal Imaging

  Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from the University of Rochester Medical Center

Pryhuber_Lab

Immunofluorescence-NKX2.1, TP63 and KRT5

 

Purpose:  Immunofluorescence on slides of paraffin sections of human lung  from the HTC for NKX2.1, TP63 and KRT5 with antigen retrieval.

Day 1

  1. Paraffin sections are placed on slide warmer for 1 hour to melt paraffin.
  2. Paraffin sections are then placed in xylene 3X for 10 minutes each, followed by 3X in 100% ETOH for 3 minutes each, 95% ETOH for 3 minutes each, and 70% ETOH for 3 minutes each. Slides are then placed in 1X PBS for 5 minutes to completely rehydrate tissue.
  3. Briefly equilibrate slides in Antigen Retrieval Buffer.
  4. Antigen retrieval, pH 6.0 (times will vary according to microwave).
  5. 10 mM sodium citrate, pH 6.0, and heat in a microwave at 96oC. *We usually do a series of three runs (6-7 minutes each run) to equal the time/temp because of evaporation (refill coplin jars with dH2O).
  6. Microwave according to instructions on microwave.
  7. Cool on countertop, 15 min.
  8. Rinse with dH2O
  9. 1X PBS, 5 min.
  10. Block in 4% Goat serum/PBS-T, 2 hours at RT.
  11. For mouse anti-TP63 (CM163A, lot # 102214, Biocare) dilute 1:100, For guinea pig anti-NKX2.1 (G237, Bl. 8/25/11) dilute 1:200 for rabbit anti-KRT5 (PRB-160P-200, Lot# D13DF01179, Biolegend) Dilute 1:100 in blocking buffer. Spin down in µfuge for 10 minutes and incubate on tissue overnight @ 4oC.

Day 2

  1. Rinse slides in PBS-T 3X, 5 min.
  2. Goat Alexa Fluor 488 anti-mouse IgG (A11001, Lot# 1298479, for anti-TP63) dilute 1:200, Goat Alexa Fluor 555 anti-guinea pig IgG (A21435, Lot# 1571699, for anti-NKX2.1) at 1:200, Goat Alexa Fluor 633 anti-rabbit IgG (A21070, Lot # 1668691, anti-KRT5) at 1:200, in blocking buffer. Spin down in µfuge for 10 min, apply to tissue and incubated at room temperature for 1 hour.
  3. Rinse in PBS-T 3X, 5 min.
  4. Dilute DAPI 1:2000 and apply to slides for 10 min.
  5. Wash in PBS-T 3X, 5 min.
  6. Rinse slides in 0.1M PB or TB, 3X, 5 min.
  7. Add 1 drop of Prolong Gold anti-fade mounting medium (P36930).
  8. Coverslip with Gold Seal Coverslip (Cat# 3422 Electron Microscopy Sciences, 22 X 22 mm).
  9. Allow Prolong Gold to cure overnight at room temp in light sealed box.

Tissue Used:
LMH-15-D007-RLL-10B2-14
Age: 20 Month Old
Gender: Male
Race: African American

 

Appendix
Antigen Retrieval Solution

18ml Solution A- 0.1M Citric Acid (Sigma, C1909) pH 2.5

82ml Reagent B- 0.1M Sodium Citrate (Sigma, S4641 ) pH 8.2

1L dH2O

pH 6.0

Related Experiments

By |2018-11-12T14:52:58+00:00October 14th, 2015|Categories: 04 Year-Old, Homo sapiens Confocal Imaging|Tags: , , , , |0 Comments
Read More

20 Month Old Human Lung TP63, NKX2.1, and KRT5 Immunofluorescence and Confocal Imaging

20 Month Old Human Lung TP63, NKX2.1, and KRT5 Immunofluorescence and Confocal Imaging

  Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from the University of Rochester Medical Center

Pryhuber_Lab

Immunofluorescence-NKX2.1, TP63 and KRT5

 

Purpose:  Immunofluorescence on slides of paraffin sections of human lung  from the HTC for NKX2.1, TP63 and KRT5 with antigen retrieval.

Day 1

  1. Paraffin sections are placed on slide warmer for 1 hour to melt paraffin.
  2. Paraffin sections are then placed in xylene 3X for 10 minutes each, followed by 3X in 100% ETOH for 3 minutes each, 95% ETOH for 3 minutes each, and 70% ETOH for 3 minutes each. Slides are then placed in 1X PBS for 5 minutes to completely rehydrate tissue.
  3. Briefly equilibrate slides in Antigen Retrieval Buffer.
  4. Antigen retrieval, pH 6.0 (times will vary according to microwave).
  5. 10 mM sodium citrate, pH 6.0, and heat in a microwave at 96oC. *We usually do a series of three runs (6-7 minutes each run) to equal the time/temp because of evaporation (refill coplin jars with dH2O).
  6. Microwave according to instructions on microwave.
  7. Cool on countertop, 15 min.
  8. Rinse with dH2O
  9. 1X PBS, 5 min.
  10. Block in 4% Goat serum/PBS-T, 2 hours at RT.
  11. For mouse anti-TP63 (CM163A, lot # 102214, Biocare) dilute 1:100, For guinea pig anti-NKX2.1 (G237, Bl. 8/25/11) dilute 1:200 for rabbit anti-KRT5 (PRB-160P-200, Lot# D13DF01179, Biolegend) Dilute 1:100 in blocking buffer. Spin down in µfuge for 10 minutes and incubate on tissue overnight @ 4oC.

Day 2

  1. Rinse slides in PBS-T 3X, 5 min.
  2. Goat Alexa Fluor 488 anti-mouse IgG (A11001, Lot# 1298479, for anti-TP63) dilute 1:200, Goat Alexa Fluor 555 anti-guinea pig IgG (A21435, Lot# 1571699, for anti-NKX2.1) at 1:200, Goat Alexa Fluor 633 anti-rabbit IgG (A21070, Lot # 1668691, anti-KRT5) at 1:200, in blocking buffer. Spin down in µfuge for 10 min, apply to tissue and incubated at room temperature for 1 hour.
  3. Rinse in PBS-T 3X, 5 min.
  4. Dilute DAPI 1:2000 and apply to slides for 10 min.
  5. Wash in PBS-T 3X, 5 min.
  6. Rinse slides in 0.1M PB or TB, 3X, 5 min.
  7. Add 1 drop of Prolong Gold anti-fade mounting medium (P36930).
  8. Coverslip with Gold Seal Coverslip (Cat# 3422 Electron Microscopy Sciences, 22 X 22 mm).
  9. Allow Prolong Gold to cure overnight at room temp in light sealed box.

 

Tissue Used:
LMH-15-D001-RLL-6C2-14
Age: 20 Month Old
Gender: Male
Race: African American

 

Appendix
Antigen Retrieval Solution

18ml Solution A- 0.1M Citric Acid (Sigma, C1909) pH 2.5

82ml Reagent B- 0.1M Sodium Citrate (Sigma, S4641 ) pH 8.2

1L dH2O

pH 6.0

Related Experiments

By |2018-11-12T14:57:09+00:00October 14th, 2015|Categories: 20 Month-Old, Homo sapiens Confocal Imaging|Tags: , , |0 Comments
Read More

9 Month Old Human Lung TP63, NKX2.1, and KRT5 Immunofluorescence and Confocal Imaging

9 Month Old Human Lung TP63, NKX2.1, and KRT5 Immunofluorescence and Confocal Imaging

  Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from the University of Rochester Medical Center

Pryhuber_Lab

Immunofluorescence-NKX2.1, TP63 and KRT5

 

Purpose:  Immunofluorescence on slides of human lung  from the HTC for NKX2.1, TP63 and KRT5 with antigen retrieval.

Day 1

  1. Paraffin sections are placed on slide warmer for 1 hour to melt paraffin.
  2. Paraffin sections are then placed in xylene 3X for 10 minutes each, followed by 3X in 100% ETOH for 3 minutes each, 95% ETOH for 3 minutes each, and 70% ETOH for 3 minutes each. Slides are then placed in 1X PBS for 5 minutes to completely rehydrate tissue.
  3. Briefly equilibrate slides in Antigen Retrieval Buffer.
  4. Antigen retrieval, pH 6.0 (times will vary according to microwave).
  5. 10 mM sodium citrate, pH 6.0, and heat in a microwave at 96oC. *We usually do a series of three runs (6-7 minutes each run) to equal the time/temp because of evaporation (refill coplin jars with dH2O).
  6. Microwave according to instructions on microwave.
  7. Cool on countertop, 15 min.
  8. Rinse with dH2O
  9. 1X PBS, 5 min.
  10. Block in 4% Goat serum/PBS-T, 2 hours at RT.
  11. For mouse anti-TP63 (CM163A, lot # 102214, Biocare) dilute 1:100, For guinea pig anti-NKX2.1 (G237, Bl. 8/25/11) dilute 1:200 for rabbit anti-KRT5 (PRB-160P-200, Lot# D13DF01179, Biolegend) Dilute 1:100 in blocking buffer. Spin down in µfuge for 10 minutes and incubate on tissue overnight @ 4oC.

Day 2

  1. Rinse slides in PBS-T 3X, 5 min.
  2. Goat Alexa Fluor 488 anti-mouse IgG (A11001, Lot# 1298479, for anti-TP63) dilute 1:200, Goat Alexa Fluor 555 anti-guinea pig IgG (A21435, Lot# 1571699, for anti-NKX2.1) at 1:200, Goat Alexa Fluor 633 anti-rabbit IgG (A21070, Lot # 1668691, anti-KRT5) at 1:200, in blocking buffer. Spin down in µfuge for 10 min, apply to tissue and incubated at room temperature for 1 hour.
  3. Rinse in PBS-T 3X, 5 min.
  4. Dilute DAPI 1:2000 and apply to slides for 10 min.
  5. Wash in PBS-T 3X, 5 min.
  6. Rinse slides in 0.1M PB or TB, 3X, 5 min.
  7. Add 1 drop of Prolong Gold anti-fade mounting medium (P36930).
  8. Coverslip with Gold Seal Coverslip (Cat# 3422 Electron Microscopy Sciences, 22 X 22 mm).
  9. Allow Prolong Gold to cure overnight at room temp in light sealed box.

 

Tissue Used:
LMH-15-D003-RLL-9B2-18
Age: 9 Month Old
Gender: Male
Race: African American

 

Appendix
Antigen Retrieval Solution

18ml Solution A- 0.1M Citric Acid (Sigma, C1909) pH 2.5

82ml Reagent B- 0.1M Sodium Citrate (Sigma, S4641 ) pH 8.2

1L dH2O

pH 6.0

Related Experiments

By |2018-11-12T14:54:48+00:00October 14th, 2015|Categories: 09 Month-Old, Homo sapiens Confocal Imaging|Tags: , , |0 Comments
Read More

Alveolar type II cells, also known as type II pneumocytes, are specialized epithelial cells found in the alveoli of the lungs, which are the tiny air sacs responsible for gas exchange. These cells play a crucial role in maintaining the structure and function of the respiratory system.

Alveolar type II cells are located within the alveolar walls of the lungs.

Alveolar type II cells are cuboidal or squamous in shape and are smaller than alveolar type I cells, which are responsible for gas exchange. They are characterized by the presence of microvilli on their apical surface, which increases their surface area for various functions.

One of the most important functions of alveolar type II cells is the production and secretion of pulmonary surfactant. Surfactant is a complex mixture of lipids and proteins that reduces the surface tension of the alveolar fluid, preventing the collapse of alveoli during exhalation. This property is crucial for maintaining the stability of the alveoli and preventing lung collapse, particularly at the end of expiration.

Alveolar type II cells serve as progenitor cells for alveolar type I cells. They have the ability to self-renew and differentiate into type I cells, helping in the repair and regeneration of the alveolar epithelium after injury or damage. This regenerative capacity is essential for maintaining the integrity of the respiratory barrier.

Alveolar type II cells also play a role in the innate immune response of the lungs. They can produce and secrete various immune molecules, including cytokines, chemokines, and antimicrobial peptides, in response to pathogens or inflammatory stimuli. These molecules help to recruit immune cells to the site of infection and promote the clearance of pathogens from the lungs.

Alveolar type II cells are involved in the regulation of ion and fluid balance within the alveoli. They express various ion channels and transporters that regulate the movement of ions, such as sodium and chloride, across the epithelial barrier. This process is important for maintaining the osmotic balance of the alveolar fluid and preventing the accumulation of fluid in the lungs (pulmonary edema).

The surfactant produced by alveolar type II cells consists mainly of phospholipids (such as dipalmitoylphosphatidylcholine, or DPPC) and surfactant proteins (SP-A, SP-B, SP-C, and SP-D). These components work together to reduce surface tension and maintain the stability of the alveoli.

The production and secretion of surfactant by alveolar type II cells are regulated by various factors, including mechanical stretch, glucocorticoid hormones, and certain signaling molecules (such as epinephrine and thyroid hormones). These regulatory mechanisms ensure that surfactant production is matched to the demands of respiration and the maintenance of lung function.

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