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Gestational Day 150 Fetal Monkey NKX2.1, MUC5AC, and MUC5B Confocal Imaging

GD150 Fetal Monkey Lung Immunofluorescence and Confocal Imaging Showing Expression of NKX2.1, MUC5AC, and MUC5B
GD150 Fetal Monkey Lung Immunofluorescence and Confocal Imaging Showing Expression of NKX2.1, MUC5AC, and MUC5B
20X
GD150 Fetal Monkey Lung Immunofluorescence and Confocal Imaging Showing Expression of NKX2.1, MUC5AC, and MUC5B
GD150 Fetal Monkey Lung Immunofluorescence and Confocal Imaging Showing Expression of NKX2.1, MUC5AC, and MUC5B
60X
GD150 Fetal Monkey Lung Immunofluorescence and Confocal Imaging Showing Expression of NKX2.1, MUC5AC, and MUC5B
GD150 Fetal Monkey Lung Immunofluorescence and Confocal Imaging Showing Expression of NKX2.1, MUC5AC, and MUC5B
60X
GD150 Fetal Monkey Lung Immunofluorescence and Confocal Imaging Showing Expression of NKX2.1, MUC5AC, and MUC5B
GD150 Fetal Monkey Lung Immunofluorescence and Confocal Imaging Showing Expression of NKX2.1, MUC5AC, and MUC5B
20X
GD150 Fetal Monkey Lung Immunofluorescence and Confocal Imaging Showing Expression of NKX2.1, MUC5AC, and MUC5B
GD150 Fetal Monkey Lung Immunofluorescence and Confocal Imaging Showing Expression of NKX2.1, MUC5AC, and MUC5B
60X
GD150 Fetal Monkey Lung Immunofluorescence and Confocal Imaging Showing Expression of NKX2.1, MUC5AC, and MUC5B
GD150 Fetal Monkey Lung Immunofluorescence and Confocal Imaging Showing Expression of NKX2.1, MUC5AC, and MUC5B
20X
GD150 Fetal Monkey Lung Immunofluorescence and Confocal Imaging Showing Expression of NKX2.1, MUC5AC, and MUC5B
GD150 Fetal Monkey Lung Immunofluorescence and Confocal Imaging Showing Expression of NKX2.1, MUC5AC, and MUC5B
60X
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By |2017-04-28T16:09:38+00:00August 16th, 2016|Categories: Fetal Monkey, GA 150|Tags: , , , |0 Comments
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Gestational Day 130 Fetal Monkey NKX2.1, MUC5AC, and MUC5B Confocal Imaging

GD130 Fetal Monkey Lung Immunofluorescence and Confocal Imaging Showing Expression of NKX2.1, MUC5AC, and MUC5B
GD130 Fetal Monkey Lung Immunofluorescence and Confocal Imaging Showing Expression of NKX2.1, MUC5AC, and MUC5B
20X
GD130 Fetal Monkey Lung Immunofluorescence and Confocal Imaging Showing Expression of NKX2.1, MUC5AC, and MUC5B
GD130 Fetal Monkey Lung Immunofluorescence and Confocal Imaging Showing Expression of NKX2.1, MUC5AC, and MUC5B
60X
GD130 Fetal Monkey Lung Immunofluorescence and Confocal Imaging Showing Expression of NKX2.1, MUC5AC, and MUC5B
GD130 Fetal Monkey Lung Immunofluorescence and Confocal Imaging Showing Expression of NKX2.1, MUC5AC, and MUC5B
60X
GD130 Fetal Monkey Lung Immunofluorescence and Confocal Imaging Showing Expression of NKX2.1, MUC5AC, and MUC5B
GD130 Fetal Monkey Lung Immunofluorescence and Confocal Imaging Showing Expression of NKX2.1, MUC5AC, and MUC5B
20X
GD130 Fetal Monkey Lung Immunofluorescence and Confocal Imaging Showing Expression of NKX2.1, MUC5AC, and MUC5B
GD130 Fetal Monkey Lung Immunofluorescence and Confocal Imaging Showing Expression of NKX2.1, MUC5AC, and MUC5B
60X
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By |2017-04-28T16:10:39+00:00August 16th, 2016|Categories: Fetal Monkey, GA130|Tags: , , , |0 Comments
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Gestational Day 105 Fetal Monkey NKX2.1, MUC5AC, and MUC5B Confocal Imaging

GD105 Fetal Monkey Lung Immunofluorescence and Confocal Imaging Showing Expression of NKX2.1, MUC5AC, and MUC5B
GD105 Fetal Monkey Lung Immunofluorescence and Confocal Imaging Showing Expression of NKX2.1, MUC5AC, and MUC5B
20X
GD105 Fetal Monkey Lung Immunofluorescence and Confocal Imaging Showing Expression of NKX2.1, MUC5AC, and MUC5B
GD105 Fetal Monkey Lung Immunofluorescence and Confocal Imaging Showing Expression of NKX2.1, MUC5AC, and MUC5B
60X
GD105 Fetal Monkey Lung Immunofluorescence and Confocal Imaging Showing Expression of NKX2.1, MUC5AC, and MUC5B
GD105 Fetal Monkey Lung Immunofluorescence and Confocal Imaging Showing Expression of NKX2.1, MUC5AC, and MUC5B
20X
GD105 Fetal Monkey Lung Immunofluorescence and Confocal Imaging Showing Expression of NKX2.1, MUC5AC, and MUC5B
GD105 Fetal Monkey Lung Immunofluorescence and Confocal Imaging Showing Expression of NKX2.1, MUC5AC, and MUC5B
60X
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By |2017-04-28T16:11:41+00:00August 16th, 2016|Categories: Fetal Monkey, GA 105|Tags: , , , |0 Comments
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20 Month Old Human Lung SCGB1A1, TUBB3, and MUC5AC Confocal Imaging

20 Month Old Human Lung SCGB1A1, TUBB3, and MUC5AC Confocal Imaging

The requested content cannot be found

  Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from the University of Rochester Medical Center

Pryhuber_Lab

Immunofluorescence-SCGB1A1, TUBB3 and MUC5AC

 

Purpose:  Immunofluorescence on slides of frozen sections of human lung  from the HTC for SCGB1A1, TUBB3 and MUC5AC with antigen retrieval.

Day 1

  1. Paraffin sections are placed at 60oC for 2 hours to overnight to melt paraffin.
  2. Paraffin sections are then placed in xylene 3X for 10 minutes each, followed by 3X in 100% ETOH for 3 minutes each, 95% ETOH for 3 minutes each, and 70% ETOH for 3 minutes each. Slides are then placed in 1X PBS for 5 minutes to completely rehydrate tissue.
  3. Antigen retrieval, pH 6.0 (times will vary according to microwave).
  4. 10 mM sodium citrate, pH 6.0, and heat in a microwave at 96oC. *We usually do a series of three runs (6-7 minutes each run) to equal the time/temp because of evaporation (refill coplin jars with dH2O).
  5. Microwave according to instructions on microwave.
  6. Cool on countertop, 15 min.
  7. Rinse with dH2O
  8. 1X PBS, 5 min.
  9. Block in 4% Goat serum/PBS-T, 2 hours at RT.
  10. For rabbit anti-SCGB1A1 (LS-B6822, Lot # 36118, LifeSpan Biosciences) dilute 1:200, for mouse anti-TUBB3 (anti-tubulin β 3, cat. # 801201, Biolegend) dilute 1:200, for mouse anti-MUC5AC (Abcam, AB3649, Lot# GR135303-1) dilute 1:200 in blocking buffer. Spin down in µfuge for 10 minutes and incubate on tissue overnight @ 4oC.

Day 2

  1. Rinse slides in PBS-T 3X, 5 min.
  2. Apply secondary antibody, Goat Alexa Fluor 488 anti-rabbit IgG (A11034, Lot# 1298480,  for anti-SCGB1A1) at 1:200, Donkey Alexa Fluor 568 anti-mouse IgG2A (A21134 for anti-TUBB3) at 1:200, Donkey Alexa Fluor 647 anti-mouse IgG1 (A21240, Lot# 1562685, anti-MUC5AC) at 1:200, in blocking buffer. Spin down in µfuge for 10 min, apply to tissue and incubated at room temperature for 1 hour.
  3. Rinse in PBS-T 3X, 5 min.
  4. Dilute DAPI 1:2000 and apply to slides for 10 min.
  5. Wash in PBS-T 3X, 5 min.
  6. Rinse slides in 0.1M PB or TB, 3X, 5 min.
  7. Add 1 drop of Prolong Gold anti-fade mounting medium (P36930).
  8. Coverslip with Gold Seal Coverslip (Cat# 3422 Electron Microscopy Sciences, 22 X 22 mm).
  9. Allow Prolong Gold to cure overnight at room temp in light sealed box.

 

Tissue Used:
LMH-15-D001-RLL-6C2-7
Age: 20 Months
Gender: Female

 

Appendix
Antigen Retrieval Solution

18ml Solution A- 0.1M Citric Acid (Sigma, C1909) pH 2.5
82ml Reagent B- 0.1M Sodium Citrate (Sigma, S4641 ) pH 8.2
1L dH2O
pH 6.0

Related Experiments

  • 20 Month Old Human Lung SCGB1A1, TUBB3, and MUC5AC Confocal Imaging
    20 Month Old Human Lung SCGB1A1, TUBB3, and MUC5AC Confocal Imaging
  • 4 Year Old Human Lung SCGB1A1, TUBB3, and MUC5AC Confocal Imaging
    4 Year Old Human Lung SCGB1A1, TUBB3, and MUC5AC Confocal Imaging
By |2018-11-12T15:55:27+00:00April 18th, 2016|Categories: 20 Month-Old, Homo sapiens Confocal Imaging|Tags: , , , |0 Comments
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9 Month Old Human Lung GRP, TUBB3, and MUC5AC Confocal Imaging

9 Month Old Human Lung GRP, TUBB3, and MUC5AC Confocal Imaging

The requested content cannot be found

  Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from the University of Rochester Medical Center

Pryhuber_Lab

Immunofluorescence-GRP, TUBB3 and MUC5AC

 

Purpose:  Immunofluorescence on slides of human lung  from the HTC for GRP, TUBB3 and MUC5AC with antigen retrieval.

Day 1

  1. Paraffin sections are placed at 60oC for 2 hours to overnight to melt paraffin.
  2. Paraffin sections are then placed in xylene 3X for 10 minutes each, followed by 3X in 100% ETOH for 3 minutes each, 95% ETOH for 3 minutes each, and 70% ETOH for 3 minutes each. Slides are then placed in 1X PBS for 5 minutes to completely rehydrate tissue.
  3. Antigen retrieval, pH 6.0 (times will vary according to microwave).
  4. 10 mM sodium citrate, pH 6.0, and heat in a microwave at 96oC. *We usually do a series of three runs (6-7 minutes each run) to equal the time/temp because of evaporation (refill coplin jars with dH2O).
  5. Microwave according to instructions on microwave.
  6. Cool on countertop, 15 min.
  7. Rinse with dH2O
  8. 1X PBS, 5 min.
  9. Block in 4% Goat serum/PBS-T, 2 hours at RT.
  10. For rabbit anti-GRP (LS-B6822, Lot # 36118, LifeSpan Biosciences) dilute 1:200, for mouse anti-TUBB3 (anti-tubulin β 3, cat. # 801201, Biolegend) dilute 1:200, for mouse anti-MUC5AC (Abcam, AB3649, Lot# GR135303-1) dilute 1:200 in blocking buffer. Spin down in µfuge for 10 minutes and incubate on tissue overnight @ 4oC.

Day 2

  1. Rinse slides in PBS-T 3X, 5 min.
  2. Apply secondary antibody, Goat Alexa Fluor 488 anti-rabbit IgG (A11034, Lot# 1298480,  for anti-GRP) at 1:200, Donkey Alexa Fluor 568 anti-mouse IgG2A (A21134 for anti-TUBB3) at 1:200, Donkey Alexa Fluor 647 anti-mouse IgG1 (A21240, Lot# 1562685, anti-MUC5AC) at 1:200, in blocking buffer. Spin down in µfuge for 10 min, apply to tissue and incubated at room temperature for 1 hour.
  3. Rinse in PBS-T 3X, 5 min.
  4. Dilute DAPI 1:2000 and apply to slides for 10 min.
  5. Wash in PBS-T 3X, 5 min.
  6. Rinse slides in 0.1M PB or TB, 3X, 5 min.
  7. Add 1 drop of Prolong Gold anti-fade mounting medium (P36930).
  8. Coverslip with Gold Seal Coverslip (Cat# 3422 Electron Microscopy Sciences, 22 X 22 mm).
  9. Allow Prolong Gold to cure overnight at room temp in light sealed box.

 

Tissue Used:
LMH-15-D003-RLL-9B2-12
Age: 9 Months
Gender: Male

 

Appendix
Antigen Retrieval Solution

18ml Solution A- 0.1M Citric Acid (Sigma, C1909) pH 2.5

82ml Reagent B- 0.1M Sodium Citrate (Sigma, S4641 ) pH 8.2

1L dH2O

pH 6.0

Related Experiments

  • 4 Month Human Lung GRP, TUBB3, and MUC5AC Confocal Imaging
    4 Month Human Lung GRP, TUBB3, and MUC5AC Confocal Imaging
  • 9 Month Old Human Lung GRP, TUBB3, and MUC5AC Confocal Imaging
    9 Month Old Human Lung GRP, TUBB3, and MUC5AC Confocal Imaging
  • 4 Year-Old Human Lung GRP, TUBB3, and MUC5AC Confocal Imaging
    4 Year-Old Human Lung GRP, TUBB3, and MUC5AC Confocal Imaging
By |2018-09-18T18:09:33+00:00April 18th, 2016|Categories: 09 Month-Old, Homo sapiens Confocal Imaging|Tags: , , |0 Comments
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4 Year Old Human Lung SCGB1A1, TUBB3, and MUC5AC Confocal Imaging

4 Year Old Human Lung SCGB1A1, TUBB3, and MUC5AC Confocal Imaging

The requested content cannot be found

  Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from the University of Rochester Medical Center

Pryhuber_Lab

Immunofluorescence-SCGB1A1, TUBB3 and MUC5AC

 

Purpose:  Immunofluorescence on slides of paraffin sections of human lung  from the HTC for SCGB1A1, TUBB3 and MUC5AC with antigen retrieval.

 Day 1

  1. Paraffin sections are placed at 60oC for 2 hours to overnight to melt paraffin.
  2. Paraffin sections are then placed in xylene 3X for 10 minutes each, followed by 3X in 100% ETOH for 3 minutes each, 95% ETOH for 3 minutes each, and 70% ETOH for 3 minutes each. Slides are then placed in 1X PBS for 5 minutes to completely rehydrate tissue.
  3. Antigen retrieval, pH 6.0 (times will vary according to microwave).
  4. 10 mM sodium citrate, pH 6.0, and heat in a microwave at 96oC. *We usually do a series of three runs (6-7 minutes each run) to equal the time/temp because of evaporation (refill coplin jars with dH2O).
  5. Microwave according to instructions on microwave.
  6. Cool on countertop, 15 min.
  7. Rinse with dH2O
  8. 1X PBS, 5 min.
  9. Block in 4% Goat serum/PBS-T, 2 hours at RT.
  10. For rabbit anti-SCGB1A1 (LS-B6822, Lot # 36118, LifeSpan Biosciences) dilute 1:200, for mouse anti-TUBB3 (anti-tubulin β 3, cat. # 801201, Biolegend) dilute 1:200, for mouse anti-MUC5AC (Abcam, AB3649, Lot# GR135303-1) dilute 1:200 in blocking buffer. Spin down in µfuge for 10 minutes and incubate on tissue overnight @ 4oC.

Day 2

  1. Rinse slides in PBS-T 3X, 5 min.
  2. Apply secondary antibody, Goat Alexa Fluor 488 anti-rabbit IgG (A11034, Lot# 1298480,  for anti-SCGB1A1) at 1:200, Donkey Alexa Fluor 568 anti-mouse IgG2A (A21134 for anti-TUBB3) at 1:200, Donkey Alexa Fluor 647 anti-mouse IgG1 (A21240, Lot# 1562685, anti-MUC5AC) at 1:200, in blocking buffer. Spin down in µfuge for 10 min, apply to tissue and incubated at room temperature for 1 hour.
  3. Rinse in PBS-T 3X, 5 min.
  4. Dilute DAPI 1:2000 and apply to slides for 10 min.
  5. Wash in PBS-T 3X, 5 min.
  6. Rinse slides in 0.1M PB or TB, 3X, 5 min.
  7. Add 1 drop of Prolong Gold anti-fade mounting medium (P36930).
  8. Coverslip with Gold Seal Coverslip (Cat# 3422 Electron Microscopy Sciences, 22 X 22 mm).
  9. Allow Prolong Gold to cure overnight at room temp in light sealed box.

 

Tissue Used:
LMH-15-D007-RLL-10B2-5
Age: 4 Years
Gender: Male

 

Appendix
Antigen Retrieval Solution

18ml Solution A- 0.1M Citric Acid (Sigma, C1909) pH 2.5
82ml Reagent B- 0.1M Sodium Citrate (Sigma, S4641 ) pH 8.2
1L dH2O
pH 6.0

Related Experiments

  • 20 Month Old Human Lung SCGB1A1, TUBB3, and MUC5AC Confocal Imaging
    20 Month Old Human Lung SCGB1A1, TUBB3, and MUC5AC Confocal Imaging
  • 4 Year Old Human Lung SCGB1A1, TUBB3, and MUC5AC Confocal Imaging
    4 Year Old Human Lung SCGB1A1, TUBB3, and MUC5AC Confocal Imaging
By |2018-11-12T15:53:39+00:00April 18th, 2016|Categories: 04 Year-Old, Homo sapiens Confocal Imaging|Tags: , , , , |0 Comments
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4 Month Human Lung KRT14, MUC5B, and MUC5AC Confocal Imaging

4 Month Human Lung KRT14, MUC5B, and MUC5AC Confocal Imaging

The requested content cannot be found

  Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from the University of Rochester Medical Center

Pryhuber_Lab

Immunofluorescence-KRT14, MUC5B and MUC5AC

 

Purpose:  Immunofluorescence on slides of frozen sections of human lung  from the HTC for KRT14, MUC5B and MUC5AC with antigen retrieval.

 Day 1

  1. Paraffin sections are placed at 60oC for 2 hours to overnight to melt paraffin.
  2. Paraffin sections are then placed in xylene 3X for 10 minutes each, followed by 3X in 100% ETOH for 3 minutes each, 95% ETOH for 3 minutes each, and 70% ETOH for 3 minutes each. Slides are then placed in 1X PBS for 5 minutes to completely rehydrate tissue.
  3. Antigen retrieval, pH 6.0 (times will vary according to microwave).
  4. 10 mM sodium citrate, pH 6.0, and heat in a microwave at 96oC. *We usually do a series of three runs (6-7 minutes each run) to equal the time/temp because of evaporation (refill coplin jars with dH2O).
  5. Microwave according to instructions on microwave.
  6. Cool on countertop, 15 min.
  7. Rinse with dH2O
  8. 1X PBS, 5 min.
  9. Block in 4% Goat serum/PBS-T, 2 hours at RT.
  10. For mouse anti-KRT14 (AB7800, Abcam) dilute 1:100, for rabbit anti-MUC5B (SC-20119, Lot # B1003 Santa Cruz) Dilute 1:100, for mouse anti-MUC5AC (Abcam, AB3649, Lot# GR135303-1) dilute 1:200 in blocking buffer. Spin down in µfuge for 10 minutes and incubate on tissue overnight @ 4oC.

Day 2

  1. Rinse slides in PBS-T 3X, 5 min.
  2. Apply secondary antibody, Goat Alexa Fluor 488 anti-mouse IgG3 (A21151, Lot# 1563682,  for anti-KRT14) at 1:200, Goat Alexa Fluor 555 anti-rabbit IgG (A21428, Lot# 1608466 for anti-MUC5B) at 1:200, Goat Alexa Fluor 647 anti-mouse IgG1 (A21240, Lot# 1562685, anti-MUC5AC) at 1:200, in blocking buffer. Spin down in µfuge for 10 min, apply to tissue and incubated at room temperature for 1 hour.
  3. Rinse in PBS-T 3X, 5 min.
  4. Dilute DAPI 1:2000 and apply to slides for 10 min.
  5. Wash in PBS-T 3X, 5 min.
  6. Rinse slides in 0.1M PB or TB, 3X, 5 min.
  7. Add 1 drop of Prolong Gold anti-fade mounting medium (P36930).
  8. Coverslip with Gold Seal Coverslip (Cat# 3422 Electron Microscopy Sciences, 22 X 22 mm).
  9. Allow Prolong Gold to cure overnight at room temp in light sealed box.

 

Tissue Used:
LMH-16-D024-RLL-6B1.7
Gender: Male
Age: 4 Months

 

Appendix
Antigen Retrieval Solution

18ml Solution A- 0.1M Citric Acid (Sigma, C1909) pH 2.5

82ml Reagent B- 0.1M Sodium Citrate (Sigma, S4641 ) pH 8.2

1L dH2O

pH 6.0

Related Experiments

  • 4 Month Human Lung KRT14, MUC5B, and MUC5AC Confocal Imaging
    4 Month Human Lung KRT14, MUC5B, and MUC5AC Confocal Imaging
By |2018-10-02T19:04:09+00:00April 15th, 2016|Categories: 04 Month-Old, Homo sapiens Confocal Imaging|Tags: , , |0 Comments
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4 Month Human Lung GRP, TUBB3, and MUC5AC Confocal Imaging

4 Month Human Lung GRP, TUBB3, and MUC5AC Confocal Imaging

The requested content cannot be found

  Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from the University of Rochester Medical Center

Pryhuber_Lab

Immunofluorescence: GRP, TUBB3, and MUC5AC 

 

Purpose:  Stain slides of 5µm frozen sections of human lung from HTC and from Pathology for GRP, TUBB3, and MUC5AC with antigen retrieval.

Day 1

  1. Paraffin sections are placed at 60oC for 2 hours to overnight to melt paraffin.
  2. Paraffin sections are then placed in xylene 3X for 10 minutes each, followed by 3X in 100% ETOH for 3 minutes each, 95% ETOH for 3 minutes each, and 70% ETOH for 3 minutes each. Slides are then placed in 1X PBS for 5 minutes to completely rehydrate tissue.
  3. Antigen retrieval, pH 6.0 (times will vary according to microwave).
  4. 10 mM sodium citrate, pH 6.0, and heat in a microwave at 96oC. *We usually do a series of three runs (6-7 minutes each run) to equal the time/temp because of evaporation (refill coplin jars with dH2O).
  5. Microwave according to instructions on microwave.
  6. Cool on countertop, 15 min.
  7. Rinse with dH2O
  8. 1X PBS, 5 min.
  9. Block in 4% Goat serum/PBS-T, 2 hours at RT.
  10. For Rabbit anti-GRP (Immunostar-order from Fisher, Cat# 20073) dilute 1:100, for mouse anti-TUBB3 (anti-tubulin β 3, cat. # 801201, Biolegend) dilute 1:200, for mouse anti-MUC5AC (Abcam, AB3649, Lot# GR135303-1) dilute 1:200 in blocking buffer. Spin down in µfuge for 10 minutes and incubate on tissue overnight @ 4oC.

Day 2

  1. Rinse slides in PBS-T 3X, 5 min.
  2. Apply secondary antibody, Goat Alexa Fluor 488 anti-rabbit IgG (A11034, Lot# 1298480,  for anti-GRP), Goat Alexa Fluor 568 anti-mouse IgG2A (A21134 for anti-TUBB3) at 1:200, Goat Alexa Fluor 647 anti-mouse IgG1 (A21240, Lot# 1562685, anti-MUC5AC) at 1:200, in blocking buffer. Spin down in µfuge for 10 min, apply to tissue and incubated at room temperature for 1 hour.
  3. Rinse in PBS-T 3X, 5 min.
  4. Dilute DAPI 1:2000 and apply to slides for 10 min.
  5. Wash in PBS-T 3X, 5 min.
  6. Rinse slides in 0.1M PB or TB, 3X, 5 min.
  7. Add 1 drop of Prolong Gold anti-fade mounting medium (P36930).
  8. Coverslip with Gold Seal Coverslip (Cat# 3422 Electron Microscopy Sciences, 22 X 22 mm).
  9. Allow Prolong Gold to cure overnight at room temp in light sealed box.

 

Tissue Used:
LMH-16-D024-RLL-6A2.16
Gender: Male
Age: 4 Months

 

Appendix
Antigen Retrieval Solution

18ml Solution A- 0.1M Citric Acid (Sigma, C1909) pH 2.5

82ml Reagent B- 0.1M Sodium Citrate (Sigma, S4641 ) pH 8.2

1L dH2O

pH 6.0

Related Experiments

  • 4 Month Human Lung GRP, TUBB3, and MUC5AC Confocal Imaging
    4 Month Human Lung GRP, TUBB3, and MUC5AC Confocal Imaging
  • 9 Month Old Human Lung GRP, TUBB3, and MUC5AC Confocal Imaging
    9 Month Old Human Lung GRP, TUBB3, and MUC5AC Confocal Imaging
  • 4 Year-Old Human Lung GRP, TUBB3, and MUC5AC Confocal Imaging
    4 Year-Old Human Lung GRP, TUBB3, and MUC5AC Confocal Imaging
By |2018-10-02T18:55:59+00:00April 15th, 2016|Categories: 04 Month-Old, Postnatal Human Lung|Tags: , , |0 Comments
Read More

20 Month Human MUC5AC, NKX2.1, and MUC5B Confocal Imaging

20 Month Human Lung Immunofluorescence and Confocal Imaging Showing Expression of MUC5AC, NKX2.1, MUC5B
20 Month Human Lung Immunofluorescence and Confocal Imaging Showing Expression of MUC5AC, NKX2.1, MUC5B
20X
20 Month Human Lung Immunofluorescence and Confocal Imaging Showing Expression of MUC5AC, NKX2.1, MUC5B
20 Month Human Lung Immunofluorescence and Confocal Imaging Showing Expression of MUC5AC, NKX2.1, MUC5B
60X
20 Month Human Lung Immunofluorescence and Confocal Imaging Showing Expression of MUC5AC, NKX2.1, MUC5B
20 Month Human Lung Immunofluorescence and Confocal Imaging Showing Expression of MUC5AC, NKX2.1, MUC5B
20X
20 Month Human Lung Immunofluorescence and Confocal Imaging Showing Expression of MUC5AC, NKX2.1, MUC5B
20 Month Human Lung Immunofluorescence and Confocal Imaging Showing Expression of MUC5AC, NKX2.1, MUC5B
60X
20 Month Human Lung Immunofluorescence and Confocal Imaging Showing Expression of MUC5AC, NKX2.1, MUC5B
20 Month Human Lung Immunofluorescence and Confocal Imaging Showing Expression of MUC5AC, NKX2.1, MUC5B
20X
20 Month Human Lung Immunofluorescence and Confocal Imaging Showing Expression of MUC5AC, NKX2.1, MUC5B
20 Month Human Lung Immunofluorescence and Confocal Imaging Showing Expression of MUC5AC, NKX2.1, MUC5B
60X
20 Month Human Lung Immunofluorescence and Confocal Imaging Showing Expression of MUC5AC, NKX2.1, MUC5B
20 Month Human Lung Immunofluorescence and Confocal Imaging Showing Expression of MUC5AC, NKX2.1, MUC5B
20X
20 Month Human Lung Immunofluorescence and Confocal Imaging Showing Expression of MUC5AC, NKX2.1, MUC5B
20 Month Human Lung Immunofluorescence and Confocal Imaging Showing Expression of MUC5AC, NKX2.1, MUC5B
60X
20 Month Human Lung Immunofluorescence and Confocal Imaging Showing Expression of MUC5AC, NKX2.1, MUC5B
20 Month Human Lung Immunofluorescence and Confocal Imaging Showing Expression of MUC5AC, NKX2.1, MUC5B
20X
20 Month Human Lung Immunofluorescence and Confocal Imaging Showing Expression of MUC5AC, NKX2.1, MUC5B
20 Month Human Lung Immunofluorescence and Confocal Imaging Showing Expression of MUC5AC, NKX2.1, MUC5B
60X
20 Month Human Lung Immunofluorescence and Confocal Imaging Showing Expression of MUC5AC, NKX2.1, MUC5B
20 Month Human Lung Immunofluorescence and Confocal Imaging Showing Expression of MUC5AC, NKX2.1, MUC5B
20X
20 Month Human Lung Immunofluorescence and Confocal Imaging Showing Expression of MUC5AC, NKX2.1, MUC5B
20 Month Human Lung Immunofluorescence and Confocal Imaging Showing Expression of MUC5AC, NKX2.1, MUC5B
60X
The requested content cannot be found

By |2018-08-17T00:39:17+00:00May 20th, 2015|Categories: 20 Month-Old, Homo sapiens Confocal Imaging|Tags: , , , |0 Comments
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