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Alcian Blue Staining of a 20 Month-Old Human Lung D011-LLL-7B2.12

Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from the University of Rochester Medical Center

Pryhuber_Lab

  1. For Frozen tissue, rinsed slides for 2X in PBS, then Incubated slides for 5 min in 4% PFA/PBS, then rinsed slides 1X in PBS.
  2. Rinsed slides for 3 minutes in running dH2O.
  3. Slides were then incubated in 3% Glacial Acetic Acid (S101, Poly Scientific) for 3 minutes.
  4. Slides were then incubated in filtered Alcian Blue (S111A, Poly Scientific) for 30 minutes.
  5. Wash slides under cold running tap water for 10 minutes.
  6. Incubate for 2 minutes in Nuclear Fast Red (S248, Poly Scientific).
  7. Wash under flowing dH2O until excess Nuclear Fast Red is washed away.
  8. Incubated slides in 90% ETOH for 20 dips, repeat in different 90% ETOH.
  9. Incubated slides in 100% ETOH for 20 dips, repeat in different 100% ETOH.
  10. Washed slides in 2X in Xylene, followed by 6 more washes in xylene.
  11. Coverslip with Permount. (Permount Toulene Solution UN 1294, Cat # SP-15-500 Fisher Scientific).

 

Tissue Used:

LMH-D011-LLL-7B2.12

Gender: Male

Age: 20 Months

By |2017-07-05T15:59:18+00:00July 5th, 2017|Categories: Alcian Blue, Postnatal Human Lung|Tags: , , |0 Comments

Alcian Blue Staining of a 8 Year-Old Human Lung D036-LLL-8B3.11

Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from the University of Rochester Medical Center

Pryhuber_Lab

  1. For Frozen tissue, rinsed slides for 2X in PBS, then Incubated slides for 5 min in 4% PFA/PBS, then rinsed slides 1X in PBS.
  2. Rinsed slides for 3 minutes in running dH2O.
  3. Slides were then incubated in 3% Glacial Acetic Acid (S101, Poly Scientific) for 3 minutes.
  4. Slides were then incubated in filtered Alcian Blue (S111A, Poly Scientific) for 30 minutes.
  5. Wash slides under cold running tap water for 10 minutes.
  6. Incubate for 2 minutes in Nuclear Fast Red (S248, Poly Scientific).
  7. Wash under flowing dH2O until excess Nuclear Fast Red is washed away.
  8. Incubated slides in 90% ETOH for 20 dips, repeat in different 90% ETOH.
  9. Incubated slides in 100% ETOH for 20 dips, repeat in different 100% ETOH.
  10. Washed slides in 2X in Xylene, followed by 6 more washes in xylene.
  11. Coverslip with Permount. (Permount Toulene Solution UN 1294, Cat # SP-15-500 Fisher Scientific).

Tissue Used:

LMH-16-D036-LLL-8B3.11

Gender: Male

Age: 8 Years

By |2019-09-10T18:18:08+00:00July 5th, 2017|Categories: Alcian Blue, Postnatal Human Lung|Tags: , , |0 Comments

Alcian Blue Staining of a 1 Day-Old Human Lung D038-LLL-8A2.45

Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from the University of Rochester Medical Center

Pryhuber_Lab

  1. For Frozen tissue, rinsed slides for 2X in PBS, then Incubated slides for 5 min in 4% PFA/PBS, then rinsed slides 1X in PBS.
  2. Rinsed slides for 3 minutes in running dH2O.
  3. Slides were then incubated in 3% Glacial Acetic Acid (S101, Poly Scientific) for 3 minutes.
  4. Slides were then incubated in filtered Alcian Blue (S111A, Poly Scientific) for 30 minutes.
  5. Wash slides under cold running tap water for 10 minutes.
  6. Incubate for 2 minutes in Nuclear Fast Red (S248, Poly Scientific).
  7. Wash under flowing dH2O until excess Nuclear Fast Red is washed away.
  8. Incubated slides in 90% ETOH for 20 dips, repeat in different 90% ETOH.
  9. Incubated slides in 100% ETOH for 20 dips, repeat in different 100% ETOH.
  10. Washed slides in 2X in Xylene, followed by 6 more washes in xylene.
  11. Coverslip with Permount. (Permount Toulene Solution UN 1294, Cat # SP-15-500 Fisher Scientific).

 

Tissue Used:

LMH-D038-LLL-8A2.45

Gender: Female

Age: 1 Day

By |2017-07-05T14:57:58+00:00July 5th, 2017|Categories: Alcian Blue, Postnatal Human Lung|Tags: , , |0 Comments

Alcian Blue Staining of a 1 Day-Old Human Lung D038-LLL-7A2.19

Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from the University of Rochester Medical Center

Pryhuber_Lab

  1. For Frozen tissue, rinsed slides for 2X in PBS, then Incubated slides for 5 min in 4% PFA/PBS, then rinsed slides 1X in PBS.
  2. Rinsed slides for 3 minutes in running dH2O.
  3. Slides were then incubated in 3% Glacial Acetic Acid (S101, Poly Scientific) for 3 minutes.
  4. Slides were then incubated in filtered Alcian Blue (S111A, Poly Scientific) for 30 minutes.
  5. Wash slides under cold running tap water for 10 minutes.
  6. Incubate for 2 minutes in Nuclear Fast Red (S248, Poly Scientific).
  7. Wash under flowing dH2O until excess Nuclear Fast Red is washed away.
  8. Incubated slides in 90% ETOH for 20 dips, repeat in different 90% ETOH.
  9. Incubated slides in 100% ETOH for 20 dips, repeat in different 100% ETOH.
  10. Washed slides in 2X in Xylene, followed by 6 more washes in xylene.
  11. Coverslip with Permount. (Permount Toulene Solution UN 1294, Cat # SP-15-500 Fisher Scientific).

 

Tissue Used:

LMH-D038-LLL-7A2.19

Gender: Female

Age: 1 Day

By |2017-06-29T15:20:11+00:00June 29th, 2017|Categories: Alcian Blue, Postnatal Human Lung|Tags: , , |0 Comments

Alcian Blue Staining of a 23 Year-Old Human Lung D042-LLL-14B3.12

Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from the University of Rochester Medical Center

Pryhuber_Lab

  1. For Frozen tissue, rinsed slides for 2X in PBS, then Incubated slides for 5 min in 4% PFA/PBS, then rinsed slides 1X in PBS.
  2. Rinsed slides for 3 minutes in running dH2O.
  3. Slides were then incubated in 3% Glacial Acetic Acid (S101, Poly Scientific) for 3 minutes.
  4. Slides were then incubated in filtered Alcian Blue (S111A, Poly Scientific) for 30 minutes.
  5. Wash slides under cold running tap water for 10 minutes.
  6. Incubate for 2 minutes in Nuclear Fast Red (S248, Poly Scientific).
  7. Wash under flowing dH2O until excess Nuclear Fast Red is washed away.
  8. Incubated slides in 90% ETOH for 20 dips, repeat in different 90% ETOH.
  9. Incubated slides in 100% ETOH for 20 dips, repeat in different 100% ETOH.
  10. Washed slides in 2X in Xylene, followed by 6 more washes in xylene.
  11. Coverslip with Permount. (Permount Toulene Solution UN 1294, Cat # SP-15-500 Fisher Scientific).

 

Tissue Used:

LMH-D042-LLL-14B3.12

Gender: Male

Age: 23 Years

By |2019-09-10T18:24:39+00:00June 29th, 2017|Categories: Adult Human Lung, Alcian Blue|Tags: , , |0 Comments

Alcian Blue Staining of a 20 Month-Old Human Lung D008-LLL-7B3.20

Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from the University of Rochester Medical Center

Pryhuber_Lab

  1. For Frozen tissue, rinsed slides for 2X in PBS, then Incubated slides for 5 min in 4% PFA/PBS, then rinsed slides 1X in PBS.
  2. Rinsed slides for 3 minutes in running dH2O.
  3. Slides were then incubated in 3% Glacial Acetic Acid (S101, Poly Scientific) for 3 minutes.
  4. Slides were then incubated in filtered Alcian Blue (S111A, Poly Scientific) for 30 minutes.
  5. Wash slides under cold running tap water for 10 minutes.
  6. Incubate for 2 minutes in Nuclear Fast Red (S248, Poly Scientific).
  7. Wash under flowing dH2O until excess Nuclear Fast Red is washed away.
  8. Incubated slides in 90% ETOH for 20 dips, repeat in different 90% ETOH.
  9. Incubated slides in 100% ETOH for 20 dips, repeat in different 100% ETOH.
  10. Washed slides in 2X in Xylene, followed by 6 more washes in xylene.
  11. Coverslip with Permount. (Permount Toulene Solution UN 1294, Cat # SP-15-500 Fisher Scientific).

 

Tissue Used:

LMH-D008-LLL-7B3.20

Gender: Female

Age: 20 Months

By |2019-09-10T17:22:33+00:00June 29th, 2017|Categories: Alcian Blue, Postnatal Human Lung|Tags: , , |0 Comments

Alcian Blue Staining of a 8 Year-Old Human Lung D036-LLL-7B2.16

Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from the University of Rochester Medical Center

Pryhuber_Lab

  1. For Frozen tissue, rinsed slides for 2X in PBS, then Incubated slides for 5 min in 4% PFA/PBS, then rinsed slides 1X in PBS.
  2. Rinsed slides for 3 minutes in running dH2O.
  3. Slides were then incubated in 3% Glacial Acetic Acid (S101, Poly Scientific) for 3 minutes.
  4. Slides were then incubated in filtered Alcian Blue (S111A, Poly Scientific) for 30 minutes.
  5. Wash slides under cold running tap water for 10 minutes.
  6. Incubate for 2 minutes in Nuclear Fast Red (S248, Poly Scientific).
  7. Wash under flowing dH2O until excess Nuclear Fast Red is washed away.
  8. Incubated slides in 90% ETOH for 20 dips, repeat in different 90% ETOH.
  9. Incubated slides in 100% ETOH for 20 dips, repeat in different 100% ETOH.
  10. Washed slides in 2X in Xylene, followed by 6 more washes in xylene.
  11. Coverslip with Permount. (Permount Toulene Solution UN 1294, Cat # SP-15-500 Fisher Scientific).

Tissue Used:

LMH-16-D036-LLL-7B2.16

Gender: Male

Age: 8 Years

By |2019-09-10T18:17:41+00:00June 29th, 2017|Categories: Alcian Blue, Postnatal Human Lung|Tags: , , |0 Comments

Alcian Blue Staining of a 6 Day-Old Human Lung D044-LLL-9A2.16

Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from the University of Rochester Medical Center

Pryhuber_Lab

  1. For Frozen tissue, rinsed slides for 2X in PBS, then Incubated slides for 5 min in 4% PFA/PBS, then rinsed slides 1X in PBS.
  2. Rinsed slides for 3 minutes in running dH2O.
  3. Slides were then incubated in 3% Glacial Acetic Acid (S101, Poly Scientific) for 3 minutes.
  4. Slides were then incubated in filtered Alcian Blue (S111A, Poly Scientific) for 30 minutes.
  5. Wash slides under cold running tap water for 10 minutes.
  6. Incubate for 2 minutes in Nuclear Fast Red (S248, Poly Scientific).
  7. Wash under flowing dH2O until excess Nuclear Fast Red is washed away.
  8. Incubated slides in 90% ETOH for 20 dips, repeat in different 90% ETOH.
  9. Incubated slides in 100% ETOH for 20 dips, repeat in different 100% ETOH.
  10. Washed slides in 2X in Xylene, followed by 6 more washes in xylene.
  11. Coverslip with Permount. (Permount Toulene Solution UN 1294, Cat # SP-15-500 Fisher Scientific).

 

Tissue Used:

LMH-D044-LLL-9A2.16

Age: 6 Days

By |2019-09-09T18:30:11+00:00June 28th, 2017|Categories: Alcian Blue, Postnatal Human Lung|Tags: , , |0 Comments

Alcian Blue Staining of a 1 Day-Old Human Lung D019-LLL-5B1.15

Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from the University of Rochester Medical Center

Pryhuber_Lab

  1. For Frozen tissue, rinsed slides for 2X in PBS, then Incubated slides for 5 min in 4% PFA/PBS, then rinsed slides 1X in PBS.
  2. Rinsed slides for 3 minutes in running dH2O.
  3. Slides were then incubated in 3% Glacial Acetic Acid (S101, Poly Scientific) for 3 minutes.
  4. Slides were then incubated in filtered Alcian Blue (S111A, Poly Scientific) for 30 minutes.
  5. Wash slides under cold running tap water for 10 minutes.
  6. Incubate for 2 minutes in Nuclear Fast Red (S248, Poly Scientific).
  7. Wash under flowing dH2O until excess Nuclear Fast Red is washed away.
  8. Incubated slides in 90% ETOH for 20 dips, repeat in different 90% ETOH.
  9. Incubated slides in 100% ETOH for 20 dips, repeat in different 100% ETOH.
  10. Washed slides in 2X in Xylene, followed by 6 more washes in xylene.
  11. Coverslip with Permount. (Permount Toulene Solution UN 1294, Cat # SP-15-500 Fisher Scientific).

 

Tissue Used:

LMH-D019-LLL-5B1.15

Gender: Male

Age: 1 Day

By |2019-09-09T16:56:18+00:00June 27th, 2017|Categories: Alcian Blue, Postnatal Human Lung|Tags: , , |0 Comments

Alcian Blue Staining of a 20 Month-Old Human Lung D011-LLL-8B3.27

Donor Tissue Kindly Provided by Dr. Gloria Pryhuber from the University of Rochester Medical Center

Pryhuber_Lab

  1. For Frozen tissue, rinsed slides for 2X in PBS, then Incubated slides for 5 min in 4% PFA/PBS, then rinsed slides 1X in PBS.
  2. Rinsed slides for 3 minutes in running dH2O.
  3. Slides were then incubated in 3% Glacial Acetic Acid (S101, Poly Scientific) for 3 minutes.
  4. Slides were then incubated in filtered Alcian Blue (S111A, Poly Scientific) for 30 minutes.
  5. Wash slides under cold running tap water for 10 minutes.
  6. Incubate for 2 minutes in Nuclear Fast Red (S248, Poly Scientific).
  7. Wash under flowing dH2O until excess Nuclear Fast Red is washed away.
  8. Incubated slides in 90% ETOH for 20 dips, repeat in different 90% ETOH.
  9. Incubated slides in 100% ETOH for 20 dips, repeat in different 100% ETOH.
  10. Washed slides in 2X in Xylene, followed by 6 more washes in xylene.
  11. Coverslip with Permount. (Permount Toulene Solution UN 1294, Cat # SP-15-500 Fisher Scientific).

 

Tissue Used:

LMH-D011-LLL-8B3.27

Gender: Male

Age: 20 Months

By |2017-06-22T16:23:39+00:00June 22nd, 2017|Categories: Alcian Blue, Postnatal Human Lung|Tags: , , |0 Comments